A research analysis of nitrogen metabolism in black-and-white cattle was carried out in the conditions of the Kuzbass region. Healthy animals at the age of 2nd lactation with a productivity level from 7300 to 10500 kg were selected for the experimental group. Laboratory studies were carried out at the Department of Veterinary Genetics and Biotechnology of the Novosibirsk State Agrarian University. Indicators of nitrogen metabolism in blood serum were assessed photometrically on a biochemical semi-automatic analyzer Photometer 5010 V5+. Reagent kits from Vector-Best and Olvex-Diagnosticum were used. When biometric data processing, standard descriptive statistics techniques and robust indicators were used. The Anderson-Darling test was used to assess the normality of data distribution. .When calculating correlation relationships, the Pearson coefficient was used. Calculations were performed in the “RStudio” programming environment. The obtained average values of nitrogen metabolism indicators were within the physiological norm. The strongest positive associations were observed between urea levels and uric acid, and urea and creatinine. No connection was found between other indicators of nitrogen metabolism. There were no differences in milk yield between cows with high and low levels of nitrogen metabolism. Graphs of the range of nitrogen content in black-and-white cows during lactation are presented. Calculations of reference indicators based on data from Kuzbass dairy cattle make it possible to more accurately assess the clinical condition of animals and timely detect deviations from established standard values. It is also necessary to take into account reference limits when monitoring the quality and completeness of cattle.
Read full abstract