The present study evaluates the sequential integration of two advanced biological treatment methods viz., sequencing batch reactor (SBR) and bioelectrochemical treatment systems (BET) for the treatment of real-field petrochemical wastewater (PCW). Initially two SBR reactors were operated in aerobic (SBRAe) and anoxic (SBRAx) microenvironments with an organic loading rate (OLR) of 9.68kg COD/m3-day. Relatively, SBRAx showed higher substrate degradation (3.34kg COD/m3-day) compared to SBRAe (2.9kg COD/m3-day). To further improve treatment efficiency, the effluents from SBR process were fed to BET reactors. BETAx depicted higher SDR (1.92kg COD/m3-day) with simultaneous power generation (17.12mW/m2) followed by BETAe (1.80kg COD/m3-day; 14.25mW/m2). Integrating both the processes documented significant improvement in COD removal efficiency due to the flexibility of combining multiple microenvironments sequentially. Results were supported with GC–MS and FTIR, which confirmed the increment in biodegradability of wastewater.