Biological Activity Of Extracts Research Articles

Biological Activity of Extracts from Aromatic Plants as Control Agents against Spoilage Molds Isolated from Sheep Cheese.

The aim of this work was to assess the antifungal and antioxidant activity of essential oils and ethanolic extracts from distilled solid by-products from aromatic plants (Artemisia dracunculus, Hyssopus officinalis, Lavandula stoechas, Origanum vulgare and Satureja montana) against 14 fungi strains isolated from sheep cheese and identified at species level using DNA barcoding based on β-tubulin sequence analysis. In addition, capacity of fungi to produce ochratoxin A, patulin, cyclopiazonic acid and sterigmatocystin was analyzed. Of the isolates, 85.7% belonged to Penicillium (P. commune/biforme, P. crustosum) and 14.3% to Aspergillus (A. puulaauensis and A. jensenii), the first time that these Aspergillus species have been found in sheep’s cheese. All P. commune isolates were producers of cyclopiazonic acid, and the two Aspergillus strains produced sterigmatocystin, but the others did not produce any tested mycotoxin. Among the essential oils tested, oregano, savory and tarragon had a significant antifungal activity against all the isolated strains, but no ethanolic extract showed antifungal activity. By contrast, ethanolic extracts showed great potential as antioxidants. The identification of new molds in cheese will help the dairy industry to know more about those molds affecting the sector, and the use of aromatic plants in the control of fungal spoilage could be a suitable alternative to chemical preservatives used in the agri-food industry.

Identification of bioactive metabolites from corn silk extracts by a combination of metabolite profiling, univariate statistical analysis and chemometrics

Corn silk has been widely used as a nutritional and medicinal supplement due to its pharmacological properties, but there is a lack of studies that correlate the extracts’ chemical composition with their biological activities. Herein, we performed the large-scale chemical characterization of corn silk extracts and used chemometrics to correlate the chemical composition with the biological activities of the extracts. Twenty-two metabolites were identified by High-Performance Liquid Chromatography coupled to Mass Spectrometry (HPLC-MS), whereas twelve were identified by Gas Chromatography coupled to Mass Spectrometry (GC–MS). Chemometrics allowed us to discriminate extracts obtained in different organic solvents from in natura and commercial product samples and to pinpoint potential candidate metabolites for the antioxidant and anti-glioma activities. Two flavone glycosides (7 and 8), along with a O-methylated anthocyanidin (26) seems to be the main contributors for the biological activities of the corn silk extracts.

BIOLOGICAL ACTIVITY OF HYPERICUM PERFORATUM L. (HYPERICACEAE): A REVIEW

Hypericum perforatum L. (St. John's wort) is a medicinal plant that has been intensivly studied by clinicians, pharmacologists, and chemists. It has resulted in the publication of both original articles and a number of, reviews devoted to the general spectrum of the biological activity of its extracts and the separate chemical components of this species. Unlike many other known medicinal plants, the pharmacological study of which is accompanied by the establishment of new (or rediscovered) structures of chemical compounds, the dynamics of the present study of H. perforatum is mostly associated with a detailed study of the mechanisms of its therapeutic effect and less with the search for new components. The aim of this work is to review and analyze the data on the biological activity of extracts and individual components of Hypericum perforatum L. (Hypericaceae), or St. John's wort, published in the scientific literature over the past 10 years. Materials and methods. To collect and analyze the information, such electronic databases as PubMed, Scopus, Web of Science, Google Scholar, and other available resources have been used. The following keywords and word combinations were used for search in the databases for 2010-2020: “ Hypericum perforatum ”, “St. John's wort”, “the biological activity of St. John's wort”, “hypericin”, “hyperforin”. Results. The review provides information on antidepressant, neuroprotective, nootropic, anxiolytic activity, antibacterial, cytotoxic, anti-inflammatory properties, analgesic, hypoglycaemic effects, and other types of activity of H. perforatum extracts, as well as individual compounds (hypericin, hyperforin, amentoflavone, and others) , isolated from this species. It is well known that the secondary metabolites of St. John's wort are naphthodianthrons, flavonoids and other phenolic compounds, several classes of lipophilic substances including phloroglucinol derivatives and terpenoids. Apart from extracts and their fractions, the biological activity of photoreactive naphthodianthrone hypericin and hyperforin (a phloroglucinol derivative) has been studied in detail. This review provides an analysis of published data from 2010 to 2020 on the biological activity of St. John's wort. At the present time H. perforatum is primarily well-known for its antidepressant-like properties, which are confirmed by numerous pharmacological studies and clinical trials. Still there is no consensus on the effective treatment of severe or even moderate depression with St. John's wort. This review also provides information on the neuroprotective, nootropic, antiepileptic, anxiolytic, antimicrobial, antiviral, antiprotozoal, antitumor, cytotoxic, analgesic, anti-inflammatory and other effects of H. perforatum extracts, as well as its individual compounds. Conclusion . Despite the popularity of H. perforatum as a plant with an antidepressant-like activity, intensive research work continues to be carried out to elucidate the molecular mechanisms of the actions of extracts and individual compounds in disorders of the nervous system. Studying its antibacterial, antiviral, and cytotoxic activity may also open up some great prospects, along with determining the possibility of using St. John's wort in metabolic disorders, genitourinary disorders, and other fields of medicine.

Chemical Screening and Biological Activities of Extracts from Cistus munbyi Pomel

Background: Cistus munbyi Pomel is a well-known medicinal plant in Western Algeria for its several applications in medicine and food. This endemic species from North Africa is poorly studied among the genus of Cistus, which gives the opportunity to carry out original pharmacological studies to value this medicinal plant. Objective: To investigate the antioxidant and the antimicrobial activities of Cistus munbyi, an endemic medicinal plant from Algeria. Methods: The antioxidant properties of C. munbyi crudes extracts were evaluated using the DPPH radical-scavenging activity tests. While their antimicrobial activity was studied by the agar diffusion method and MICs determination against eighteen bacterial and fungal strains, some of which were clinical. Results: The obtained results showed that aqueous and ethanol extracts possess a strong scavenging activity against DPPH radicals with EC50 values estimated at 1.12 and 8.31 μg/mL, respectively. On the other hand, extracts from C. munbyi revealed homogeneous and good antimicrobial activity, in which the inhibition zone diameters observed were ranging from 08 to 19 mm, and the MICs values were included from 0.312 to 5.000 mg/mL. The aqueous extract was less active except against Proteus mirabilis and Staphylococcus aureus. Conclusion: The findings of this study indicate that C. munbyipossesses a strong antioxidant activity and an interesting antimicrobial potential, which allows justifying its uses by the local population in several applications. Therefore, C. munbyi could be a good source of natural antioxidants and antimicrobial agents.

Artemisia abrotanum L. (Southern Wormwood)-History, Current Knowledge on the Chemistry, Biological Activity, Traditional Use and Possible New Pharmaceutical and Cosmetological Applications.

Artemisia abrotanum L. (southern wormwood) is a plant species with an important position in the history of European and Asian medicine. It is a species famous as a medicinal plant in Central Asia, Asia Minor, and in South-East and Central Europe. The raw materials obtained from this species are Abrotani herba and Abrotani folium. In the traditional European medicine, they have been used successfully most of all in liver and biliary tract diseases, in parasitic diseases in children and as antipyretic medication. In the official European medicine, this plant species is recommended by the French Pharmacopoeia for use in homeopathy. In many European countries, it is used traditionally in allopathy. The latest studies on the biological activity of extracts from the aboveground parts of the plant and/or the leaves, and/or the essential oil have provided evidence of other possible applications related to their antibacterial, antifungal, antioxidant, anticancer, and antiallergic properties. The latest studies have also focused on the repellent activity of the essential oil of this species and the possibility to use it in the prevention of diseases in which insects are the vectors. The main substances obtained from the plant that are responsible for this activity are: the essential oil, coumarins, phenolic acids, and flavonoids. Some of the latest investigations emphasize the large differences in the composition of the essential oil, determined by the geographical (climatic) origin of the plant. A. abrotanum is recommended by the European Cosmetic Ingredients Database (CosIng) as a source of valuable cosmetic ingredients. Additionally, the leaves of this species possess a well-established position in the food industry. This plant species is also the object of biotechnological studies.

Various Biological Activities of Extracts from Deodeok (Codonopsis lanceolata Trautv.) Buds

Ji Yeong Kim, Byung Soon Hwang, Su Hyun Kwon, Mi Jang, Gi Chang Kim, Hae Ju Kang, and In Guk Hwang. Journal of the Korean Society of Food Science and Nutrition 2021;50:10-5. https://doi.org/10.3746/jkfn.2021.50.1.10

Root Extracts of Two Cultivars of Paeonia Species: Lipid Composition and Biological Effects on Different Cell Lines: Preliminary Results.

The roots of two cultivars of Paeonia, namely Paeonia officinalis “Rubra Plena” and Paeonia “Pink Hawaiian Coral”, have been extracted with chloroform. The composition of the lipid fraction, analyzed by GC–MS technique, revealed the absence of paeonol and the presence of phenol, benzoic acid, fatty acid—and some sterol—derivatives. The chloroformic extracts have been tested on normal and several cancer cell lines but showed antiproliferative activity only on the ovarian carcinoma and the osteosarcoma. The biological activity of extracts was investigated mainly by confocal microscopy, flow cytometry and quantum phase imaging. The results indicated that the root extracts induced a hyperpolarization of mitochondria and an increase in reactive oxygen species levels, without inducing cell death. These effects are associated to an increased doubling time and a retarded confluence.

Phytochemical Analysis and Evaluation of Antioxidant and Biological Activities of Extracts from Three Clauseneae Plants in Northern Thailand.

This study established the DNA barcoding sequences (matK and rbcL) of three plant species identified in the tribe Clauseneae, namely Clausena excavata, C. harmandiana and Murraya koenigii. The total phenolic and total flavonoid contents, together with the biological activities of the derived essential oils and methanol extracts, were also investigated. Herein, the success of obtaining sequences of these plant using two different barcode genes matK and rbcL were 62.5% and 100%, respectively. Both regions were discriminated by around 700 base pairs and these had resemblance with those of the Clausenae materials earlier deposited in Genbank at a 99–100% degree of identity. Additionally, the use of matK DNA sequences could positively confirm the identity as monophyletic. The highest total phenolic and total flavonoid content values (p < 0.05) were observed in the methanol extract of M. koenigii at 43.50 mg GAE/g extract and 66.13 mg QE/g extract, respectively. Furthermore, anethole was detected as the dominant compound in C. excavata (86.72%) and C. harmandiana (46.09%). Moreover, anethole (26.02%) and caryophyllene (21.15%) were identified as the major phytochemical compounds of M. koenigii. In terms of the biological properties, the M. koenigii methanol extract was found to display the greatest amount of antioxidant activity (DPPH; IC50 95.54 µg/mL, ABTS value 118.12 mg GAE/g extract, FRAP value 48.15 mg GAE/g extract), and also revealed the highest α-glucosidase and antihypertensive inhibitory activities with percent inhibition values of 84.55 and 84.95. Notably, no adverse effects on human peripheral blood mononuclear cells were observed with regard to all of the plant extracts. Furthermore, M. koenigii methanol extract exhibited promise against human lung cancer cells almost at 80% after 24 h and 90% over 48 h.

Biological Activities of Extracts from Leaf of Angelica gigas Nakai

Biological Activities of Extracts from Leaf of Angelica gigas Nakai

Toxic action of substances from male fern Dryopteris filix-mas (L.) Schott (1834) on free-living soil nematode Caenorhabditis elegans Maupas (1900)

The study of biological activity of extracts from roots and rhizomes of male fern Dryopteris filix-mas was carried out in experiments with soil nematode Caenorhabditis elegans. The toxicity of extracts of D. filix-mas roots and rhizomes obtained by different methods varied over a wide range. Crude extract of male fern roots and rhizomes in concentration range 62.5–250 µg/ml had a weak toxic action on C. elegans organism by inducing death of 26.0–43.3% nematodes. The toxicity of relatively high concentrations (500 and 250 µg/ml) of water-ethanolic extracts might be compared with such of crude extract in concentrations 62.5–250 µg/ml. Biological activity of filicinic acids ethers was found very high: concentrations of crude extract of D. filix-mas roots and rhizomes and concentrations of filicinic acids ethers with similar toxicity for C. elegans organism were 40-fold different. Substances isolated from D. filix-mas roots and rhizomes have nematicidal activity and may be used for development of drugs to treat helminthiasis of humans and animals.

Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

Biological activities of extracts from Tongue fern (Pyrrosia lingua)

Biological activities of extracts from Tongue fern (<i>Pyrrosia lingua</i>)

Selective enrichment of ailanthone from leaves of ailanthus altissima by tandem reverse phase/molecularly imprinted solid phase extraction

The biological activity of extracts from Ailanthus altissima is mainly due to the presence of ailanthone, a compound belonging to the quassinoid class. Recently, attention has been paid to its strong cytostatic activity. However, the extraction of ailanthone is based on very long and demanding procedures, which keep the price of the commercial product very high. Thus, the development of selective adsorbents for the purification of ailanthone from A. altissima leaves extracts could help in reduce the costs of production. In this work, we describe the rational design of a molecularly imprinted polymer selective for ailanthone based on the screening of a 96-members not-imprinted polymeric library to rapidly identify pre-polymerization mixtures able to generate MIPs with enhanced binding properties. A 4-vinylpyridine-co-trimethylolpropane trimethacrylate polymer showed high binding towards ailanthone. It was used to prepare an imprinted polymer with acetonitrile showed interesting binding affinity (Keq = 18.3 × 103 L mol−1), high imprinting factor (IF = 3.8) and fast binding kinetics (kass = 0.390 min−1, kdis = 0.021 mol L−1 min−1). The imprinted polymer was used to develop a successful purification protocol of extracts from Ailanthus altissima leaves. The purification was based on the combination of a preliminary clean-up of Soxhlet extracts onto a reverse phase-C18 cartridge and the subsequent isolation of ailanthone by a molecularly imprinted solid phase extraction. This approach allowed efficiently purifying the ailanthone contained in aqueous or methanolic Soxhlet extracts with high yields compared to the quantities reported in literature (water: 0.756 ± 0.027 mg g−1; methanol: 0.770 ± 0.030 mg g−1). Moreover, it allows processing sample volumes up to 15 mL without significant losses of the target compound.

Antiparasitic activity of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides

Parasitoses are very common throughout the world, generating serious consequences for public health. Leishmaniosis and giardiasis are examples of fairly recurrent, but neglected diseases. Several higher plants have demonstrated promising activity against the parasites. The aim of this study was to evaluate the biological activity of extracts, fractions and isolated compounds from the leaves and stems of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides (Myrtaceae) against Leishmania and Giardia. XTT and the fluorimetric method were used to for this evaluation, respectively. Cytotoxicity was evaluated against HeLa cells. The results demonstrated that chloroform fractions of E. matosii and pinostrobin presented the most pronounced antiparasitic activity, with the CLF-stems being the most effective against Leishmania amazonensis and Leishmania braziliensis. Pinostrobin also presented activity against G. lamblia. Therefore, E. mattosii stems and pinostrobin may be considered possible targets for the continuity of studies against other parasites.

Chemical Composition and Biological Activities of Extracts from Pomelo Peel By-Products under Enzyme and Ultrasound-Assisted Extractions

Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) were popular methods used to extract bioactive compounds from citrus peels, by-products of fruit processing industry. In this study, the total phenolic content (TPC), total flavonoid content (TFC), naringin and hesperidin contents, and antioxidant and antimicrobial activities of the extracts from pomelo peels using the combined enzyme and ultrasound-assisted extraction (E-UAE) or ultrasound and enzyme-assisted extraction (U-EAE) technique were investigated and compared with those extracted using the EAE and UAE. The optimal EAE conditions were as follows: enzyme concentration of 2%, water-solid ratio of 40 ml/g, incubation temperature of 50°C, and extraction time of 60 min, whereas the optimal UAE conditions were ultrasonic energy of 40 kHz, water-solid ratio of 40 ml/g at room temperature, and extraction time of 60 min. The results indicate that the total phenolics, total flavonoids, naringin, and hesperidin contents of the extracts significantly increased in the following order of the extraction techniques: UAE &lt; EAE &lt; U-EAE &lt; E-UAE (p&lt;0.05). The combined E-UAE technique was the most effective technique for bioactive compound extraction with the highest antioxidant and antimicrobial activities. The results also indicate that chemical composition and antioxidant and antimicrobial activities of the extracts were different depending on the pomelo species.

Phytochemical Analysis, Multi-Element Composition and Biological Activities of Extracts and Lupenone from Albizia inundata (Mart.) Barneby &amp; J.W.Grimes

In this study, antimicrobial activity of extracts and lupenone from A. inundata (Fabaceae, Mimosoideae), were tested. In addition, the multi-element composition of the leaves, bark and stems (heartwood) of A. inundata was evaluated using inductively coupled plasma optical emission spectrometry (ICP OES). Lupenone was isolated in the petroleum ether extract of leaves, for the first time. Biological assays were conducted to evaluate the antimicrobial (antibacterial, antifungal and antiparasitic) of extracts, their fractions and lupenone. The results showed antimicrobial and antileishmanial activity of these compounds, which may be useful for obtaining a chemical composition and biological activity database on Albizia species. For multi-element composition, we found the mean concentrations (minimum-maximum) of Al, Ca, Cr, Cu, Fe, K, Mg, Mn, Ni, P, V and Zn in the samples ranged between 0.06 and 265.41 µg g-1. Concentration ranges of Cu, Fe, Mn and Zn are large, which makes these samples as additional sources of these macronutrients.

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF METABOLITES OF THE GENUS ALHAGI

In the review summarized the scientific literature on geographical distribution, the degree of knowledge of the chemical composition and biological activity of extracts and isolated individual compounds of the genus Alhagi Tourn. ex Adans. of the world flora. The structures of the isolated 300 compounds are presented belonging to the alkaloids, terpenoids, carbohydrates, hydrocarbons, lipids, and phenolic compounds. In quantitative terms, the phenolic compounds dominate, among which flavonoids belonging to the group flavone, flavonol, flavanone, isoflavone, isoflavonolignan and flavan-3-ols groups are prevails. Among them, to the chemotoxonomic markers can be include flavonols narcissin and its aglycone isorhamnetin, which are produced in major quantities in practically all species of the genus Alhagi. It was revealed that the qualitative composition of the metabolites of the same species of this genus depends on the ecological-geographical and soil-climatic conditions of their growth place. The data on the biological activity of extracts and isolated individual metabolites are considered. The main biologically active substances of the genus Alhagi are phenolic compounds. The presented information in the review shows that the plants of the genus Alhagi are promising for the creation of new pharmaceuticals. It was noted that in present time are being conducted in-depth preclinical pharmacological studies of the antioxidant preparation "Yantacin", the anti-inflammatory "Alkakhin", the biologically active complex "Alkhidin", and the antioxidant and antibacterial preparation "Zhantharid". Cited in the review information may be used as reference literature by phytochemists, biologists, and pharmacologists.

Total Phenolic and Flavonoid Content and Biological Activities of Extracts and Isolated Compounds of Cytisus villosus Pourr.

The aim of this study was to evaluate the total phenolic and flavonoid content, and the in vitro antioxidant, anti-inflammatory, antibacterial, antifungal, antimalarial, cytotoxicity, and antiprotozoal activities of the Algerian plant Cytisus villosus Pourr. (Syn. Cytisus triflorus L’Hérit.). Additionally, the radioligand displacement affinity on opioid and cannabinoid receptors was assessed for the extracts and isolated pure compounds. The hydro alcoholic extract of the aerial part of C. villosus was partitioned with chloroform (CHCl3), ethyl acetate (EtOAc), and n-butanol (n-BuOH). The phenolic content of the C. villosus extracts was evaluated using a modified Folin–Ciocalteau method. The total flavonoid content was measured spectrometrically using the aluminum chloride colorimetric assay. The known flavonoids genistein (1), chrysin (2), chrysin-7-O-β-d-glucopyranoside (3), and 2″-O-α-l-rhamnosylorientin (4) were isolated. The antioxidant activities of the extracts and isolated compounds were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant activity (CAA) assays. The plant extracts showed moderate antioxidant activity. EtOAc and n-BuOH extracts showed moderate anti-inflammatory activity through the inhibition of induced nitric oxide synthase (iNOS) with IC50 values of 48 and 90 µg/mL, respectively. The isolated pure compounds 1 and 3 showed good inhibition of Inducible nitric oxide synthase (iNOS) with IC50 values of 9 and 20 µg/mL, respectively. Compounds 1 and 2 exhibited lower inhibition of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) with IC50 values of 28 and 38 µg/mL, respectively. Furthermore, the extracts and isolated pure compounds have been shown to exhibit low affinity for cannabinoid and opioid receptors. Finally, n-BuOH extract was a potent inhibitor of Trypanosoma brucei with IC50 value of 7.99 µg/mL and IC90 value of 12.61 µg/mL. The extracts and isolated compounds showed no antimicrobial, antimalarial nor antileishmanial activities. No cytotoxic effect was observed on cancer cell lines. The results highlight this species as a promising source of anti-inflammatory and antitrypanosomal agents.

Antioxidant, iron chelating, lipase inhibition activities and metabolite’s prediction of hyrdoethanolic leaf extract of Conocarpus erectus

The current study was conducted to evaluate the antioxidant, Fe-chelating and lipase inhibition activities of hydroethanolic leaf extracts of Conocarpus erectus. The proton magnetic resonance spectroscopy (1H-NMR) was used to identify the major types of primary and secondary metabolites in extract. The 60% ethanolic extract was found to be the most effective fraction regarding antioxidant, Fe chelating and lipase inhibitory properties. The 60% ethanolic leaf extract exhibited total antioxidant power of 223.0±3.27 mg ASE/g PE, β-carotene bleaching inhibition of 81.39±2.11%, Fe-chelating of 68.21±1.17% and pancreatic lipase inhibition of 50.60±1.47%, respectively. The 1H-NMR -based prediction of metabolites provided the information for presence of polyphenolic secondary metabolites and organic acids which might be responsible for the biological activities of extract. These findings of this work may be extended for metabolite characterization, in vivo trials and toxicity determination to get benefits for functional food development with antioxidative and antiobesity attributes.

Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

Annona nutans (Annonaceae) is a plant species found in Bolivia, Paraguay, Argentina, and the Brazilian Cerrado, specifically in the states of Mato Grosso and Mato Grosso do Sul (Brazil). Its common names are Araticû-Mi and Araticû-Ñu. The research contributions regarding the chemical composition and biological activities of extracts from A. nutans are rare, with only four articles being found in the literature. Therefore, the present study evaluated the anti-inflammatory and antinociceptive activities of the hydromethanolic fraction (FHMeOH) using carrageenan-induced paw edema and hot-plate tests. In addition, the antioxidant activity was evaluated by DPPH radical scavenging, total phenolic, flavonoid and tannin content assays and quantification of the major metabolites by LC-MS were performed. Oral treatment with the FHMeOH (at a dose of 300 mg.kg-1) significantly reduced paw edema 2 h and 4 h after the inflammatory stimulus. The intraperitoneal (i.p.) treatment with the FHMeOH (50 and 100 mg.kg-1) proved to be most effective, and the inhibition of acute inflammation was still visible 6 h after carrageenan injection. At doses of 50 and 100 mg.kg-1 (i.p.), FHMeOH exhibits central antinociceptive effects by increasing the latency of the reaction in the hot-plate model. The FHMeOH showed antioxidant potential, and the metabolites quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apiofuranosyl-(1→2)-galactopyranoside, and chlorogenic acid were identified and quantified by LC-MS. Our results indicate, for the first time, that FHMeOH has anti-inflammatory, antinociceptive and antioxidant potential, and it is a promising source of studies for new herbal medicines