Biological Activated Carbon Filtration Research Articles

Risk assessment and inactivation of invertebrate-internalized bacteria in pilot-scale biological activated carbon filtration

It is well documented that invertebrates can ingest and transport pathogenic bacteria, thus protecting the bacteria against disinfection in the laboratory. However, the risk assessment of and corresponding disinfection methods for natural invertebrate-internalized bacteria in biological activated carbon (BAC) filtration systems remain poorly understood. In this study, the risk of natural invertebrate-internalized bacteria was comprehensively assessed and methods to inactivate these bacteria were compared in a pilot-scale BAC filtration column study lasting one year. Seven groups of invertebrates dominated by rotifers and crustaceans were detected in the filtration column, five of which were collected for quantitative/qualitative identification of the bacteria they internalized. The community composition of internalized bacteria was analyzed via polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) coupled with sequence analysis of 16S rRNA gene fragments. Results showed that the average numbers of internalized bacteria per organism ranged from 160 to 6000, which increased exponentially with invertebrate body length. Some of the invertebrate-internalized bacteria were identified as opportunistic human pathogens, but no direct human pathogens were detected. A model was developed to calculate the residual bacteria concentration. Using this model, it was determined that an average of 800–100,000 CFU/m3 internalized bacteria would be protected and then released into the distribution mains after chlorination of 50 mg/L·min, with rotifers and copepods the dominant sources. Ozonation was more effective than both chlorination and UV radiation for inactivating the invertebrate-internalized bacteria.

Transformation and fate of dissolved organic nitrogen in drinking water supply system: A full scale case study from Yixing, China

The transformation of dissolved organic nitrogen (DON) in the drinking water treatment plants could be closely associated with nitrogenous disinfection by-product (N-DBP) formation. In this study, we have assessed the molecular transformation of DON and its impact on N-DBP formation in a full scale drinking water treatment plant. Based on the result of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) analysis, DON compounds with low molecular weight (<1 kDa) were classified as CHON, CHON2 and CHON3 according to the number of nitrogen atoms. Via the analytical window of van Krevelen diagrams, we found that the molecular structural features of CHON, CHON2 and CHON3 were not altered before the chlorination process. In detail, the CHON2 and CHON3 compositions were concentrated on the regions assigned to a lignin-structure while CHON compositions were also distributed in other compounds including proteins, carbohydrates and tannin. Furthermore, CHON formation was more difficult to be removed before the V-filter process. For N-DBP, chlorine-containing DON (Cl-DON) composition was likely to be removed through flocculation and sedimentation processes, whereas N-nitrosamine compounds were removed in V-filter and biological activated carbon filter processes. The health risks of aromatic structure N-nitrosamines due to the pre-chlorination of the raw water should be further studied.

Impact of temperature and dissolved oxygen level on the population dynamics of naidids and their reproduction in biological activated carbon filters: a life table demographic study

Abstract Aquatic macro-organisms, such as naidids, propagate excessively in biological activated carbon (BAC) filters. This has become a troublesome problem for drinking water plants. For successful control of naidid contamination risk, it is necessary to determine the population dynamics under different environmental conditions within drinking water plants, with special emphasis on BAC filters. In this study, field studies of naidid distribution in a drinking water plant were conducted, and the effects of temperature and dissolved oxygen (DO) on naidid population dynamics were investigated using the life table method. The results indicated that naidid pollution in the water plant occurred seasonally and was induced by the excessive propagation of naidids in the BAC filters. Increased temperature and DO increased the naidid intrinsic rate of natural increase and decreased the naidid population doubling time. The life table method was also used to acquire the reproductive parameters of naidids in BAC filters based on simulative experiments. These results indicated that naidids can reproduce asexually in BAC filters, and the population doubling time was 12.60 days.

Temperature-dependent mechanisms of DOM removal by biological activated carbon filters

Physical and chemical adsorption by aged biological active carbon (BAC) filters were observed for some organic matter fractions, and may represent important removal mechanisms during periods of low microbial activity.

Growth competition between ammonia-oxidizing archaea and bacteria for ammonium and urea in a biological activated carbon filter used for drinking water treatment

By applying time-course DNA-SIP, the substrate niche separation of AOA and AOB was revealed. AOA had higher autotrophic growth activity for a low concentration of ammonium, while AOB directly incorporated and hydrolyzed urea.

Effect of biological activated carbon filter depth and backwashing process on transformation of biofilm community

The biological activated carbon (BAC) is a popular advanced water treatment to the provision of safe water supply. A bench–scale device was designed to gain a better insight into microbial diversity and community structure of BAC biofilm by using high–throughput sequencing method. Both samples of BAC biofilm (the first, third and fifth month) and water (inlet water and outlet water of carbon filter, outlet water of backwashing) were analyzed to evaluate the impact of carbon filter depth, running time and backwash process. The results showed that the microbial diversity of biofilm decreased generally with the increase of carbon filter depth and biofilm reached a steady–state at the top layer of BAC after three months’ running. Proteobacteria (71.02%–95.61%) was found to be dominant bacteria both in biofilms and water samples. As one of opportunistic pathogen, the Pseudomonas aeruginosa in the outlet water of device (1.20%) was about eight times higher than that in the inlet water of device (0.16%) at the genus level after five–month operation. To maintain the safety of drinking water, the backwash used in this test could significantly remove Sphingobacteria (from 8.69% to 5.09%, p<0.05) of carbon biofilm. After backwashing, the operational taxonomic units (OTUs) number and the Shannon index decreased significantly (p<0.05) at the bottom of carbon column and we found the Proteobacteria increased by about 10% in all biofilm samples from different filter depth. This study reveals the transformation of BAC biofilm with the impact of running time and backwashing.

Effects of the inclusion of biological activated carbon on membrane fouling in combined process of ozonation, coagulation and ceramic membrane filtration for water reclamation

We investigated the effects of the inclusion of biological activated carbon (BAC) on membrane fouling in combined process of ozonation, coagulation and ceramic membrane filtration (O3 + PACl + CMF) for treating secondary effluent. Inclusion of BAC between ozonation and coagulation reduced membrane permeability. The normalized flux decreased to 90% of the initial value after 305 h of operation in O3 + PACl + CMF, while it decreased to 20% in combined process of ozonation, BAC, coagulation and ceramic membrane filtration. BAC not only decreased residual ozone that is helpful to mitigate ceramic membrane fouling, but also released microorganisms. In addition, BAC doubled the integrated fluorescence intensity of soluble microbial products (SMP), which cause irreversible fouling. The SMP produced and accumulated by microorganisms on the BAC bed likely flowed into the BAC effluent with the microorganisms. The proportion of SMP in the extracted foulant increased from 25% without BAC to 31% with BAC. Moreover, the inclusion of BAC nearly doubled the concentration of protein in the extracted foulant to 13 g/m2 and quadrupled that of carbohydrate to 6 g/m2. BAC was effective in improving the quality of ceramic membrane permeates and reducing health risk associated with formaldehyde and N-nitrosodimethylamine. However, the release of SMP from BAC accelerated membrane fouling in subsequent ceramic membrane filtration.

Effects of water quality changes on performance of biological activated carbon (BAC) filtration

Biological activated carbon (BAC) filtration is an important treatment step in the production of drinking water especially if drinking water is produced from surface water. The performance and processes within a BAC filter have been of interest for researchers since the 1980’s, mainly because of its ability to remove natural organic matter known as disinfection precursors. A malfunction of one of the pre-treatment steps might affect the feed water quality into the BAC filters. The main objective of this study was to determine the immediate response of the BAC filters to a rapid change in feed water quality. It was shown that with the studied setup it was possible to compare the effect of different pre-treatment steps and subsequent different water qualities on the performance of the BAC filters on the long term adaptation. However, especially the immediate response was not studied in detail before. All filters were able to mitigate a sudden change in feed water quality, either through improved adsorption or increased activity of the biomass on the filter. As a result of this resilience against sudden changes, it is therefore concluded that there is no direct need for very stringent on-line monitoring and continuous adjustments of the feed water quality of the BAC filters. The addition of phosphate resulted in the lowest dissolved organic carbon (DOC) concentration in the effluent of the BAC filters. In this study the influence of intact cells in the feed water on the performance of the BAC filters was shown to be limited.

Characteristics of Bacterial Communities in Biological Filters of Full-Scale Drinking Water Treatment Plants

The taxonomic and functional characteristics of bacterial communities in the pre-chlorinated rapid filters and ozonated biological activated carbon (BAC) filters were compared using Illumina MiSeq sequencing of the 16S rRNA gene and community-level physiological profiling (CLPP) based on sole-carbon-source utilization patterns. Both the rapid filters and BAC filters were dominated by Rhizobiales within α-proteobacteria, but other abundant orders and genera were significantly different in both types of filter. Firmicutes were abundant only in the intermediate chlorinated rapid filter, while Acidobacteria were abundant only in the BAC filters. Bacterial communities in the rapid filter showed high utilization of carbohydrates, while those in the BAC filters showed high utilization of polymers and carboxylic acids. These different characteristics of the bacterial communities could be related to the different substrates in the influents, filling materials, and residual disinfectants. Chlorination and ozonation inactivated the existing bacteria in the influent and formed different bacterial communities, which could be resistant to the oxidants and effectively utilize different substrates produced by the oxidant, including Phreatobacter in the rapid filters and Hyphomicrobium in the BAC filters. Bradyrhizobium and Leptothrix, which could utilize compounds adsorbed on the GAC, were abundant in the BAC filters. Ozonation increased taxonomic diversity but decreased functional diversity of the bacterial communities in the BAC filters. This study provides some new insights into the effects of oxidation processes and filling materials on the bacterial community structure in the biological filters of drinking water treatment plants.

Removal of perfluoroalkyl and polyfluoroalkyl substances in potable reuse systems

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a group of persistent contaminants that have been identified throughout the aquatic environment. In this study, ten targeted perfluoroalkyl acids (PFAAs), three targeted PFAA precursors, and non-targeted PFAA precursors were monitored in four full- and pilot-scale potable reuse plants at each stage of advanced treatment. Non-targeted PFAA precursors were quantified by applying a total oxidizable precursor assay in which PFAA precursors are oxidized by hydroxyl radicals to targeted PFAAs. Two of the potable reuse systems had membrane-based treatments with reverse osmosis and UV-advanced oxidation (RO-UV/AOP) and two used ozone, biological activated carbon filtration and granular activated carbon adsorption (O3-BAC-GAC). The total targeted PFAAs in the four tertiary effluents, the influent sources for the potable reuse systems, ranged from 52 to 227 ng/L with non-targeted PFAA precursors accounting for 30–67% of total PFASs on a molar basis. The RO-UV/AOP treatment trains reduced PFAAs and PFAA precursors to below their method reporting limits through the barrier provided by RO. The O3-BAC-GAC based treatment trains reduced, but did not completely remove PFAAs or PFAA precursors and the PFASs present in the product water were primarily shorter-chain PFAAs, some of which lack human health guidance values for drinking water. The relative fraction of targeted shorter-chain PFAAs increased after each treatment step indicating that there was preferential removal of the PFAA precursors and longer-chain PFAAs. This study provides new insight on the concentrations and treatment of PFAA precursors through potable reuse treatment systems.

Removal of precursors of typical nitrogenous disinfection byproducts in ozonation integrated with biological activated carbon (O3/BAC)

The O3/BAC process has been widely used in drinking water treatment to improve the removal of dissolved organic matters (DOMs), including the precursors of nitrogenous disinfection byproducts (N-DBPs). In this study, the removal of N-DBP precursors by biological activated carbon (BAC) filters with different usage time of granular activated carbon (GAC) was investigated. Results showed that the BAC filter with 6 years of usage time of GAC (old BAC filter) had a poor performance in the removal of precursors of N-DBPs such as dichloroacetonitrile (DCAN; an average of only 4.7%), dichloroacetamide (DCAcAm), and trichloronitromethane (TCNM) when compared with the BAC filter with 1 year of usage time of GAC (new BAC filter). Particularly, the organic fraction >10 kDa and the percentage of autochthonous substances were increased in the effluent of the old BAC filter. The red shift of the fluorescence peaks was evident in the excitation–emission matrix spectrum of the effluent from the old BAC filter. The abiotic adsorption of precursors by the old BAC filter was less. In addition, less amino acids and polysaccharides were removed, but more amino sugars and proteins were produced because of microbial metabolism. The metabolism strength of the attached biofilm decreased with increased operation time of the BAC filter. The relative abundance of Sphingomonas significantly decreased in the biofilm of the old BAC filter. The diversity of microbial community in the old BAC filter was higher, but the equitability was lower than those of the new BAC filter. The less removal of N-DBP precursors by the old BAC filter was attributed to the changes in abiotic adsorption capacity and microbial metabolism properties, in which soluble microbial products played an important role.

Advanced biological activated carbon filter for removing pharmaceutically active compounds from treated wastewater

Through their release of effluents, conventional wastewater treatment plants (WWTPs) represent a major pollution point sources for pharmaceutically active compounds (PhACs) in water bodies. The combination of a biological activated carbon (BAC) filter coupled with an ultrafiltration (UF) unit was evaluated as an advanced treatment for PhACs removal at pilot scale. The BAC-UF pilot plant was monitored for one year. The biological activity of the biofilm that developed on the granular activated carbon (GAC) particles and the contribution of this biofilm to the overall removal of PhACs were evaluated. Two different phases were observed during the long-term monitoring of PhACs removal. During the first 9200 bed volumes (BV; i.e., before GAC saturation), 89, 78, 83 and 79% of beta-blockers, psychiatric drugs, antibiotics and a mix of other therapeutic groups were removed, respectively. The second phase was characterized by deterioration of the overall performances during the period between 9200 and 13,800 BV. To quantify the respective contribution of adsorption and biodegradation, a lab-scale setup was operated for four months and highlighted the essential role played by GAC in biofiltration units. Physical adsorption was indeed the main removal mechanism. Nevertheless, a significant contribution due to biological activity was detected for some PhACs. The biofilm contributed to the removal of 22, 25, 30, 32 and 35% of ciprofloxacin, bezafibrate, ofloxacin, azithromycin and sulfamethoxazole, respectively.

Leakage of soluble microbial products from biological activated carbon filtration in drinking water treatment plants and its influence on health risks

The application of ozone-biological activated carbon (O3-BAC) as an advanced treatment method in drinking water treatment plants (DWTPs) can help to remove organic micropollutants and further decrease the dissolved organic carbon (DOC) level in finished water. With the increase attention to microbial safety of drinking water, a pre-positioned O3-BAC followed by a sand filter has been implanted into DWTP located in Shanghai, China to increase the biostability of effluents. The results showed that BAC had high removal efficiencies of UV254, DOC and disinfection by-product formation potential (DBPFP). The removal efficiencies between pre- and post-positioned BAC filtrations were similar. Based on the analyses of fluorescence excitation–emission matrix spectrophotometry (FEEM), the generation and leakage of soluble microbial products (SMPs) were found in both two BAC filtrations on account of the increased fluorescence intensities and fluorescence regional integration (FRI) distribution of protein-like organics, as well as the enhanced biological index (BIX). The leakage of SMPs produced by metabolism of microbes during BAC process resulted in increased DBPFP yield and carcinogenic factor per unit of DOC (CF/DOC). Although BAC filtration reduced the DBPFP and CF, there still was high health risk of effluents for the production of SMPs. Therefore, the health risks for SMPs generated by BAC filtration in drinking water advanced treatment process should be addressed, especially with that at high temperature.

Organic matter removal in bio-filters to achieve biologically stable water downstream of the process

Abstract Organic removal contaminants reclamation has increased over the past years as it has many advantages over other treatments. Product water from water treatment plants can be chemically complex resulting in physical and biological changes during transportation in the distribution systems. The general aim of this study was to evaluate the effectiveness of biofilters for reducing the concentration of organic matter in order to produce biologically stable water, avoiding biofouling formation downstream of the process units. Enhanced coagulation and media filters of expanded clay, sand, and biological activated carbon (BAC) have been assessed. PH and coagulant dose have been optimized to achieve maximum turbidity and organic removal. Filtration stages along the operation have been monitored, measuring parameters such DOC (Dissolved Organic Carbon), UVabs (ultraviolet absorbance), BDOC (Biodegradable Organic Carbon) and AOC (Assimilable Organic Carbon). Once the biological stage was achieved and the organic removal was constant and steady along the process units, the BDOC analysis showed evidence of the outcomes of each filtration system, with BAC filters in conjunction with enhanced coagulation giving outstanding performance.

Changes in Dissolved Organic Matter Composition and Disinfection Byproduct Precursors in Advanced Drinking Water Treatment Processes.

Molecular changes in dissolved organic matter (DOM) from treatment processes at two drinking water treatment plants in Japan were investigated using unknown screening analysis by Orbitrap mass spectrometry. DOM formulas with carbon, hydrogen and oxygen (CHO-DOM) were the most abundant class in water samples, and over half of them were commonly found at both plants. Among the treatment processes, ozonation induced the most drastic changes to DOM. Mass peak intensities of less saturated CHO-DOM (positive (oxygen subtracted double bond equivalent per carbon (DBE-O)/C)) decreased by ozonation, while more saturated oxidation byproducts (negative (DBE-O)/C) increased and new oxidation byproducts (OBPs) were detected. By Kendrick mass analysis, ozone reactions preferred less saturated CHO-DOM in the same alkylation families and produced more saturated alkylation families of OBPs. Following ozonation, biological activated carbon filtration effectively removed <300 Da CHO-DOM, including OBPs. Following chlorination, over 50 chlorinated formulas of disinfection byproducts (DBPs) were found in chlorinated water samples where at least half were unknown. Putative precursors of these DBPs were determined based on electrophilic substitutions and addition reactions. Ozonation demonstrated better decomposition of addition reaction-type precursors than electrophilic substitution-type precursors; over half of both precursor types decreased during biological activated carbon filtration.

Performance of ozonation and biological activated carbon in eliminating sulfonamides and sulfonamide-resistant bacteria: A pilot-scale study

Removal of sulfonamides (SAs) by an integrated ozonation and biological activated carbon filtration (O3-BAC) process, as an advanced drinking water treatment process, was evaluated in a pilot-scale study. Furthermore, the effects of the O3-BAC process on sulfonamide-resistant bacteria (SRB) and on the removal of total organic carbon, UV254, and turbidity were investigated. Results indicate that ozonation could effectively remove SAs from water at an appropriate ozone dose with an improvement of water quality; however, BAC filtration would lead to an increase of SRB. In addition, the performance of the integrated process in eliminating disinfection byproducts formation potential (DBPsFP) in chlorination was evaluated. The results show ozonation would result in an increase of DBPsFP, while subsequent BAC filtration could effectively reduce DBPsFP to the safety-standard level of water quality. Overall, effective removal was achieved by the O3-BAC process in terms of the reduction of SAs but not of SRB. Subsequent disinfection is significant and biological safety should be evaluated further to ensure the effectiveness of the integrated process and the security of water quality.

Effect of sedimentation time on the granulometric composition of suspended solids in the backwash water from biological activated carbon filters

The paper presents the results of analyzes of the granulometric composition of suspended solids in backwash water from biological activated carbon (BAC) filters and its changes during sedimentation. Backwash water samples were taken during backwashing of two pilot filters after different filtration time. It was found that regardless of the concentration of suspended solids in the collected backwash water, particle sizes vs. their percentage volume contributions for all samples were similar. Particle sizes were in the range of 2–100 μm. However, the two-hour sedimentation for most of the samples proved to be effective (total suspended solids removal up to 93%), which is caused by self-coagulation of the sample.

Removal of tryptophan in drinking water using biological activated carbon filter

Abstract Tryptophan (Trp), an important nitrogenous organic compound commonly present in water sources and posing a serious threat to human health, was selected as the main object in the removal trial by utilizing a laboratory-scale biological activated carbon (BAC) column. The laboratory-scale BAC column was divided in a three-part composition: upper layer (UL) (0–20 cm), middle layer (ML) (20–40 cm) and bottom layer (BL) (40–60 cm). The removal efficiencies of Trp in the three layers were 45.4%, 86.4% and 43.2%, respectively, while the adsorption of granular activated carbon (GAC) for Trp did not show a similar tendency (the average adsorption yields were 10.98 ± 1.17 mg/g, 7.45 ± 0.80 mg/g and 3.32 ± 0.39 mg/g, respectively), which indicated that the biodegradation of microorganisms attached to the GAC played an important role. Furthermore, a high-throughput quantitative polymerase chain reaction (HT-qPCR) was utilized to determine the relative abundance of the first eight bacterial genera in the three BAC layers and results revealed the relative abundance of Aquincola, Pseudomonas and Ensifer were highest in the ML.

Influence of the type of activated carbon on invertebrate leakage in biological activated carbon filter

Influence of the type of activated carbon on invertebrate leakage in biological activated carbon filter

Optimization of the precursor removal of dichloroacetonitrile (DCAN), an emerging nitrogenous disinfection by-product, in an up-flow BAC filter

The process parameters of the up-flow biological activated carbon filter (UBACF) were optimized in a pilot-scale trial for controlling the precursor of dichloroacetonitrile (DCAN), an emerging nitrogenous disinfection by-product. The experiments were performed using a central composite design (CCD) with the response surface methodology (RSM). The results showed that the removal efficiencies of formation potentials (FP) of DCAN increased from 28.9% to 64.4% with the optimized ozone dose, expansion rate of BAC and backwashing cycle, being scheduled to 1.52 mg/L, 27% and 9.5 d, respectively. Excitation and emission matrix (EEM) spectra indicated that the fluorescence peaks of aromatic protein (AP) and soluble microbial products-like (SMPs)-like region were weakened significantly in the effluent of improved process (IP) with optimization, which were main precursors of DCAN. The bacterial community before and after the optimization of UBACF was determined using the high-throughput sequencing technology. The class and genus of microorganism demonstrated that the IP had a more diverse microbial community and more even distribution of species in BAC filter. It was favor of the growth of Alphaproteobacteria, Bacilli and Betaproteobacteria attached to the BAC particles, which could biodegrade effectively the precursors of DCAN.