The aim of study is reduction of accumulation of heavy metals in agrocenosis of pulse crops due to the action of biologically active substances in the conditions of the middle Volga forest. An experiment study in order to reveal the effect of pre-sowing seed treatment with biologically active substances on the accumulation of heavy metals by soils and plants in agrocenoses of peas and soybeans was conducted in 2013-2015. The results of pre – sowing treatment of soybean seeds of the Samer 3 breed and peas of the Flagman 12 with the Rizotorfin and Rizotorfin in combination with analogues – Agrica and Humariz biologically active substance are presented. The analysis of heavy metals content in soil and plant samples was performed in the laboratory of the «Samara agrochemical ser-vice» Station by method of atomic adsorption spectroscopy. It is established that the introduction of biologically ac-tive substances into the soil containing heavy metals can reduce the concentration of their mobile forms and restrict access to grain. The content of mobile forms of elements in the soil under soybean growing areas, in comparison with the control, decreases: lead by 1.14 times when treated with Rhizotorphin+Humariz, cadmium – 1.18 times when treated with Rhizotorphin, copper – 1.2 times and cobalt – 2.0 times under the influence of the combination of Rhizotorphin and Agrica. According to the coefficients of biological absorption of pea and soy grains, the studied heavy metals are referred to the elements of biological capture of CАB<1. An effective inactivating effect on heavy metals is provided by pre-sowing treatment of pea and soy seeds with biologically active substances combined with Rhizotorphin+Agrica and Rhizotorphin+Humariz.