Biofilm Reactor Research Articles

Performance and reaction kinetics of NO absorption by Fe(II)EDTA in a multistage zeolite absorption column-H2-MBfR system

Efficient NOX complexation absorption is a key to biological flue gas denitrification. In this study, the influencing factors, efficiency, and kinetic parameters of Fe(II)EDTA absorption of NO in a multistage zeolite absorption column were evaluated. The response surface methodology (RSM) was used to determine the ideal absorption conditions. Based on these results, a combined process of a multistage zeolite absorption column and a hydrogen-based membrane biofilm reactor (H2-MBfR) was built to verify the efficiency of denitrification. The optimal conditions for achieving maximum efficiency in the absorption of NO by Fe(II)EDTA were obtained as pH of 6.5, Fe(II)/EDTA molar ratio of 1:1.1, and temperature of 30 ℃. The kinetic parameters, including liquid phase mass transfer coefficient (KNO,L) and gas phase mass transfer coefficient (KNO,G), all increased with temperature, while the NO interfacial-area concentration (aNO) exhibited the opposite trend. The reaction for Fe(II)EDTA absorption of NO was a conjugation reaction, and its activation energy was 50.38 kJ·mol-1. More than 90 % of NO in flue gas on 14 days was removed in the combined multistage zeolite absorption column-H2-MBfR process, which provided a sustainable NO treatment solution without secondary pollutants or CO2 emissions and demonstrated its potential for NO removal in industrial applications.

WITHDRAWN: An Investigation of a Hybrid Solar-Mineral Disinfection Technique Using Zeolite and Dead Sea Clay

Effluents from water treatment plants (WTP) can often contain a complex mixture of residual micro-contaminants and organisms, not removed during wastewater treatment. A combined trickling filter, activated sludge treatment, ozonation, membrane filtration, and suspended biofilm reactors have been shown to reduce these contaminants in waste effluent but at high capita cost. This study examined an inexpensive method of purifying water, which combines solar water disinfection with natural mineral clays (SOMIN-DIS). The study involved the addition of two types of mineral clays, Zeolite and Dead Sea minerals, to containers of polluted wastewater, at varying concentrations. The containers were then subjected to different durations of sunlight exposure. Then, samples were taken from all of the containers to quantify the overall numbers of Total Coliform, Pseudomonas aeruginosa (P.aeruginosa), and Escherichia coli (E.coli), utilizing the IDEXX system. The findings indicated that incorporating Dead Sea clay and Zeolite into SODIS-treated wastewater decreased the overall number of harmful microorganisms and shortened the disinfection duration, as compared to using natural minerals and solar water disinfection (SODIS) independently. Additionally, the study determined that the most effective concentration of Dead Sea minerals, resulting in the least amounts of harmful microorganisms and shortest purification duration, was 0.001g/ml. In contrast, the optimum concentration of Zeolite was 0.002g/ml. In general, the addition of Dead Sea and Zeolite minerals to the wastewater increased the inactivation of bacteria under SODIS from 40% to 65%.

Enhanced AHL-mediated quorum sensing accelerates the start-up of biofilm reactors by elevating the fitness of fast-growing bacteria in sludge and biofilm communities

Quorum sensing (QS)-based manipulations emerge as a promising solution for biofilm reactors to overcome challenges from inefficient biofilm formation and lengthy start-ups. However, the ecological mechanisms underlying how QS regulates microbial behaviors and community assembly remain elusive. Herein, by introducing different levels of N-acyl-homoserine lactones, we manipulated the strength of QS during the start-up of moving bed biofilm reactors and compared the dynamics of bacterial communities. We found that enhanced QS elevated the fitness of fast-growing bacteria with high ribosomal RNA operon (rrn) copy numbers in their genomes in both the sludge and biofilm communities. This led to notably increased extracellular substance production, as evidenced by strong positive correlations between community-level rrn copy numbers and extracellular proteins and polysaccharides (Pearson's r = 0.529−0.830, P < 0.001). Network analyses demonstrated that enhanced QS significantly promoted the ecological interactions among taxa, particularly cooperative interactions. Bacterial taxa with higher network degrees were more strongly correlated with extracellular substances, suggesting their crucial roles as public goods in regulating bacterial interactions and shaping network structures. However, the assembly of more cooperative communities in QS-enhanced reactors came at the cost of decreased network stability and modularity. Null model and dissimilarity-overlap curve analysis revealed that enhanced QS strengthened stochastic processes in community assembly and rendered the universal population dynamics more convergent. Additionally, these shaping effects were consistent for both the sludge and biofilm communities, underpinning the planktonic-to-biofilm transition. This work highlights that QS manipulations efficiently drive community assembly and confer specialized functional traits to communities by recruiting taxa with specific life strategies and regulating interspecific interactions. These ecological insights deepen our understanding of the rules governing microbial societies and provide guidance for managing engineering ecosystems.

Effects of microorganisms on nitrogen and phosphorus removal from wastewater

Nitrogen and phosphorus are key to plant growth. The balance of nitrogen and phosphorus is crucial in nature. Excessive nitrogen and phosphorus content in wastewater has caused great harm to the environment. Traditional nitrogen and phosphorus removal methods can no longer meet the treatment standards for nitrogen and phosphorus. This article introduces the hazards of nitrogen and phosphorus, and points out the harm to nature and human beings if they are not up to standard in wastewater treatment plants. Through the analysis and discussion of several methods, the advantages of microbial nitrogen and phosphorus removal are explained. This article further summarizes several representative new microorganisms and microbial processes through the types and mechanisms of microbial nitrogen and phosphorus removal. Anammox bacteria can accept other electron pools to increase nitrogen removal efficiency. Denitrifying phosphorus-removing bacteria can achieve energy saving and carbon reducing effects through endogenous denitrification. Besides, the enhanced biological phosphorus removal process can allow denitrifying phosphorus accumulating bacteria to absorb phosphorus in an anaerobic, anoxic, aerobic alternating environment. The moving bed biofilm reactor (MBBR) abandons the disadvantages of the traditional biofilm method, while retaining the advantages of the traditional biofilm method such as shock load resistance and low sludge production. These new microorganisms and new microbial processes have become important applications in future nitrogen and phosphorus removal processes due to their advantages of high efficiency, low energy, and no secondary pollution. This article is conducive to the promotion of microbial-based wastewater treatment and can provide suggestions for the sustainable development of wastewater treatment.

Experimental investigation of simultaneous nitrification-denitrification and phosphorus removal in pilot-scale sequencing batch moving bed biofilm reactors (SB-MBBRs)

Sequencing batch moving bed biofilm reactors have been widely used in commercial wastewater treatment facilities for organic carbon and nitrogen removal. However, these reactors can remove low phosphorus (P) levels. Therefore, this study investigated the potential of SB-MBBRs for maximizing simultaneous nitrification-denitrification and P removal (SNDPR) potential from P-rich municipal wastewater impacted by industrial discharges. A series of experiments were carried out to investigate the effect of external volatile fatty acid (VFA) dosing, airflow rate, and temperature on SNDPR using pilot-scale SBMBBRs. Stable and robust SNDPR was achieved with an optimum acetic acid supply of 150 mg SCOD/L, at 20 oC and 2.5 L air/min. A low airflow rate (AFR) and high-temperature conditions affected P release and uptake kinetics. Efficient PHA storage, dissolved oxygen (DO) transfer (outer layer), DO diffusion limitation (inner layer) of biofilm, and conversion of NH4-N to NO2-N/NO3-N enhanced SNDPR in the two pilot SB-MBBRs.

Superior mainstream partial nitritation in an acidic membrane-aerated biofilm reactor

Shortcut nitrogen removal holds significant economic appeal for mainstream wastewater treatment. Nevertheless, it is too difficult to achieve the stable suppression of nitrite-oxidizing bacteria (NOB), and simultaneously maintain the activity of ammonia-oxidizing bacteria (AOB). This study proposes to overcome this challenge by employing the novel acid-tolerant AOB, namely “Candidatus Nitrosoglobus”, in a membrane-aerated biofilm reactor (MABR). Superior partial nitritation was demonstrated in low-strength wastewater from two aspects. First, the long-term operation (256 days) under the acidic pH range of 5.0 to 5.2 showed the successful NOB washout by the in situ free nitrous acid (FNA) of approximately 1 mg N/L. This was evidenced by the stable nitrite accumulation ratio (NAR) close to 100 % and the disappearance of NOB shown by 16S rRNA gene amplicon sequencing and fluorescence in situ hybridization. Second, oxygen was sufficiently supplied in the MABR, leading to an unprecedentedly high ammonia oxidation rate (AOR) at 2.4 ± 0.1 kg N/(m3 d) at a short hydraulic retention time (HRT) of a mere 30 min. Due to the counter diffusion of substrates, the present acidic MABR displayed a significantly higher apparent oxygen affinity (0.36 ± 0.03 mg O2/L), a marginally lower apparent ammonia affinity (14.9 ± 1.9 mg N/L), and a heightened sensitivity to FNA and pH variations, compared with counterparts determined by flocculant acid-tolerant AOB. Beyond supporting the potential application of shortcut nitrogen removal in mainstream wastewater, this study also offers the attractive prospect of intensifying wastewater treatment by markedly reducing the HRT of the aerobic unit.

Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat.

Reducing production costs, known as scaling, is a significant obstacle in the advancement of cultivated meat. The cultivation process hinges on several key components, e.g., cells, media, scaffolds, and bioreactors. This study demonstrates an innovative approach, departing from traditional stainless steel or glass bioreactors, by integrating food-grade plant-based scaffolds and thermoplastic film bioreactors. While thermoplastic films are commonly used for constructing fluidic systems, conventional welding methods are cost-prohibitive and lack rapid prototyping capabilities, thus inflating research and development expenses. The developed laser welding technique facilitates contamination-free and leakproof sealing of polyethylene films, enabling the efficient fabrication of macrofluidic systems with various designs and dimensions. By incorporating food-grade plant-based scaffolds, such as rice seeded with bovine mesenchymal stem cells, into these bioreactors, this study demonstrates sterile cell proliferation on scaffolds within macrofluidic systems. This approach not only reduces bioreactor prototyping and construction costs but also addresses the need for scalable solutions in both research and industrial settings. Integrating single-use bioreactors with minimal shear forces and incorporating macro carriers such as puffed rice may further enhance biomass production in a scaled-out model. The use of food-grade plant-based scaffolds aligns with sustainable practices in tissue engineering and cultured-meat production, emphasizing its suitability for diverse applications.

Resource-Efficient Nitrogen Removal from Source-Separated Urine with Partial Nitritation/Anammox in a Membrane-Aerated Biofilm Reactor

Resource-Efficient Nitrogen Removal from Source-Separated Urine with Partial Nitritation/Anammox in a Membrane-Aerated Biofilm Reactor

Downflow sponge biofilm reactors for polluted raw water treatment: Performance optimisation, kinetics, and microbial community

Water outages caused by elevated ammonium (NH4+-N) levels are a prevalent problem faced by conventional raw water treatment plants in developing countries. A treatment solution requires a short hydraulic retention time (HRT) to overcome nitrification rate limitation in oligotrophic conditions. In this study, the performance of polluted raw water treatment using a green downflow sponge biofilm (DSB) technology was evaluated. We operated two DSB reactors, DSB-1 and DSB-2 under different NH4+-N concentration ranges (DSB-1: 3.2–5.0 mg L−1; DSB-2: 1.7–2.6 mg L−1) over 360 days and monitored their performance under short HRT (60 min, 30 min, 20 min, and 15 min). The experimental results revealed vertical segregation of organic removal in the upper reactor depths and nitrification in the lower depths. Under the shortest HRT of 15 min, both DSB reactors achieved stable NH4+-N and chemical oxygen demand removal (≥95%) and produced minimal effluent nitrite (NO2−-N). DSB system could facilitate complete NH4+-N oxidation to nitrate (NO3−-N) without external aeration energy requirement. The 16S rRNA sequencing data revealed that nitrifying bacteria Nitrosomonas and Nitrospira in the reactor were stratified. Putative comammox bacteria with high ammonia affinity was successfully enriched in DSB-2 operating at a lower NH4+-N loading rate, which is advantageous in oligotrophic treatment. This study suggests that a high hydraulic rate DSB system with efficient ammonia removal could incorporate ammonia treatment capability into polluted raw water treatment process and ensure safe water supply in many developing countries.

A novel membrane-aerated biofilm reactor using flexible and adjustable plate membrane modules: Treatment of wastewater containing anionic surfactants

Membrane-aerated biofilm reactors (MABRs) are a promising biofilm technology for wastewater treatment; however, achieving high pollutant removal in MABR systems with a low specific membrane surface area, low air-based aeration pressure and flexible aeration control remains a substantial challenge. In this study, a flexible and adjustable plate membrane module was developed and applied in an air-based plate MABR (PMABR) for treating anionic surfactant wastewater. The plate membrane module achieved high oxygen mass transfer under a low air-based membrane aeration pressure. When the influent linear alkylbenzene sulfonate (LAS) load was 0.559±0.010 kg/m3·d and a gradient-pressure membrane aeration scheme of (8,7,6) kPa was adopted, the LAS removal rate reached 99.2±0.1%, and the LAS removal load per unit membrane area reached 14.08±0.25 g/m2·d. The bacteria participating in the degradation of LAS were mainly Parvibaculum, Pseudomonas, Desulfovibrio, Flavobacterium and Holophagaceae. The dominant microbial communities of different biofilms in the PMABR played an important role in ω-oxidation, β-oxidation, benzene ring breaking and sulfonic acid group shedding in the degradation of LAS. The developed plate membrane module and PMABR provide a clean, energy-saving and flexible technical solution for MABR applications in wastewater treatment.

Dissolved oxygen dependence of Microbe-Derived dissolved organic nitrogen dynamics during membrane biofilm-based linear alkylbenzene sulfonate mineralization

Oxygen-based membrane biofilm reactor (O2-MBfR) can achieve bubble-free efficient linear alkylbenzene sulfonate (LAS) mineralization. However, effluent microbially derived dissolved organic nitrogen (mDON) reduces recycled water quality and induces water eutrophication. How dissolved oxygen (DO) impacts the dynamics of mDON during LAS biodegradation is unknown. Here, we explored the fate of mDON releasing, molecular characteristics and formation metabolisms in the O2-MBfR responding to DO dynamics using Fourier transform ion cyclotron resonance mass spectrometry and metagenomic analyses. Results showed that DO presents a negative impact (λ = -0.242, P < 0.001) on mDON production, but can positively influence mDON reduction through influencing total organic nitrogen (TDN) removal. DO of 0.3 mg/L led to the lowest concentration of mDON with the least number of organic molecular formulas and high proportion of stable N2– and N3-containing organics. Much lower (0.0 mg/L) or higher (0.6 mg/L) DO concentrations favored dissimilatory-based ammonia formation and assimilatory nitrogen fixation that enhancing mDON formation. Firmocutes were the dominant bacteria that contributing to mDON formation. TDN (λ = 0.844, P < 0.001) and pH (λ = 0.248, P < 0.01) directly affected the effluent mDON concentration. The relatively low effluent mDON under anoxic condition suggested that DO controlling can be an effective strategy to minimize bioavailable mDON discharges in the O2-MBfR, which showed great implication potential for high-quality reclaimed water production and eutrophication control.

Simultaneous removal of ammonia nitrogen, calcium and cadmium in a biofilm reactor based on microbial-induced calcium precipitation: Optimization of conditions, mechanism and community biological response

With the enhancement of environmental governance regulations, the discharge requirements for reverse osmosis wastewater have become increasingly stringent. This study proposes an innovative approach utilizing heterotrophic nitrification and aerobic denitrification (HNAD)-based biomineralization technology, combined with coconut palm silk loaded biochar, to offer a novel solution for resource-efficient and eco-friendly treatment of reverse osmosis wastewater. Zobellella denitrificans sp. LX16 were loaded onto modified coir silk and showed removal efficiencies of up to 97.38, 94.58, 86.24, and 100% for NH4+-N (65 mg L−1), COD (900 mg L−1), Ca2+ (180 mg L−1), and Cd2+ (25 mg L−1). Analysis of the metabolites of microorganisms reveals that coconut palm silk loaded with deciduous biochar (BCPS) not only exerts a protective effect on microorganisms, but also enhances their growth, metabolism, and electron transfer capabilities. Characterization of precipitation phenomena elucidated the mechanism of Cd2+ removal via ion exchange, precipitation, and adsorption. Employing high-throughput and KEGG functional analyses has confirmed the biota environmental response strategies and the identification of key genes like HNAD.

Comparison of nitrification performance in SBR and SBBR with response to NaCl salinity shock: Microbial structure and functional genes

Ammonia removal by nitrifiers at the extremely high salinity poses a great challenge for saline wastewater treatment. Sequencing batch reactor (SBR) was conducted with a stepwise increase of salinity from 10 to 40 g-NaCl·L−1, while sequencing batch biofilm reactor (SBBR) with one-step salinity enhancement, their nitrification performance, microbial structure and interaction were evaluated. Both SBR and SBBR can achieve high-efficiency nitrification (98% ammonia removal) at 40 g-NaCl·L−1. However, SBBR showed more stable nitrification performance than SBR at 40 g-NaCl·L−1 after a shorter adaptation period of 4–15 d compared to previous studies. High-throughput sequencing and metagenomic analysis demonstrated that the abundance and capability of conventional ammonia-oxidizing bacteria (Nitrosomonas) were suppressed in SBBR relative to SBR. Gelidibacter, Anaerolineales were the predominant genus in SBBR, which were not found in SBR. NorB and nosZ responsible for reducing NO to N2O and reducing N2O to N2 respectively had s strong synergistic effect in SBBR. This study will provide a valuable reference for the startup of nitrification process within a short period of time under the extremely high NaCl salinity.

Computational modeling of microalgal biofilm growth in heterogeneous rotating algal biofilm reactors (RABRs) for wastewater treatment

Rotating algal biofilm reactors (RABRs) are innovative systems designed to cultivate microalgae biofilms efficiently. In this paper, we have developed a novel mathematical model to accurately capture the growth dynamics of algae biofilms within RABR. By considering the spatial heterogeneity of the RABR, we introduce a PDE-based model that addresses the spatial variations across the substratum, enabling a more accurate simulation of biofilm growth in RABRs. The photosynthesis process is modeled through reactive kinetics, driving the growth of the algae biofilm. To analyze the system's behavior, we employ finite difference numerical methods to solve the complex PDE model. We then conduct extensive numerical simulations to understand algae biofilm growth in the RABR environment under various operational factors and environmental conditions. One primary focus in these simulations is to investigate the impact of various harvesting strategies, harvesting frequencies, light intensity, and light exposure on the overall biomass productivity of the algae biofilm. The numerical results provide valuable insights into optimizing algae biofilm growth and designing harvesting techniques in RABR systems. Our proposed novel mathematical model provides an effective platform for the theoretical investigation and design of RABRs for wastewater treatment.

Innovative inbuilt moving bed biofilm reactor for nitrogen removal applied in household aquarium.

An innovative inbuilt moving bed biofilm reactor (MBBR) was created to protect fish from nitrogen in a household aquarium. During the 90 experimental days, the ammonia nitrogen (NH4+-N) concentration in the aquarium with the inbuilt MBBR was always below 0.5 mg/L, which would not threaten the fish. Concurrently, nitrite and nitrate nitrogen concentrations were always below 0.05 mg/L and 4.5 mg/L, respectively. However, the blank contrast aquarium accumulated 1.985 mg/L NH4+-N on the 16th day, which caused the fish to die. The suspended biofilms could achieve the specific NH4+-N removal rate of 45.43 g/m3/d. Biofilms presented sparsely with filamentous structures and showed certain degrees of roughness. The bacterial communities of the suspended biofilms and the sediment were statistically different (p < 0.05), reflected in denitrifying and nitrifying bacteria. In particular, the relative abundance of Nitrospira reached 1.4%, while the genus was barely found in sediments. The suspended biofilms showed potentials for nitrification function with the predicted sequence numbers of ammonia monooxygenase [1.14.99.39] and hydroxylamine dehydrogenase [EC:1.7.2.6] of 220 and 221, while the values of the sediment were only 5 and 1. This study created an efficient NH4+-N removal inbuilt MBBR for household aquariums and explored its mechanism to afford a basis for its utilization.

Comparative Efficacy of Continuous Ceftazidime Infusion vs. Intermittent Bolus against In Vitro Ceftazidime-Susceptible and -Resistant Pseudomonas aeruginosa Biofilm.

Background: As the anti-biofilm pharmacokinetic/pharmacodynamic (PK/PD) properties of antibiotics are not well-defined, we have evaluated the PK/PD indices for different regimens of ceftazidime (CAZ; with/without colistin) against Pseudomonas aeruginosa biofilm. Methods: We have used the Center for Disease Control and Prevention Biofilm Reactor with two susceptible (PAO1 and HUB-PAS) and one resistant (HUB-XDR) strains of P. aeruginosa. The regimens were CAZ monotherapies (mimicking a human dose of 2 g/8 h, CAZ-IB; 6 g/daily as continuous infusion at 50 mg/L, CAZ-CI50; and 9 g/daily at 70 mg/L, CAZ-CI70) and CAZ-colistin combinations. Efficacy was correlated with the CAZ PK/PD parameters. Results: CAZ-CI70 was the most effective monotherapy against CAZ-susceptible strains (Δlog CFU/mL 54-0 h = -4.15 ± 0.59 and -3.05 ± 0.5 for HUB-PAS and PAO1, respectively; p ≤ 0.007 vs. other monotherapies), and adding colistin improved the efficacy over CAZ monotherapy. CAZ monotherapies were ineffective against the HUB-XDR strain, and CAZ-CI50 plus colistin achieved higher efficacy than CAZ-IB with colistin. The PK/PD index that correlated best with anti-biofilm efficacy was fAUC0-24h/MIC (r2 = 0.78). Conclusions: CAZ exhibited dose-dependent anti-biofilm killing against P. aeruginosa, which was better explained by the fAUC0-24h/MIC index. CAZ-CI provided benefits compared to CAZ-IB, particularly when using higher doses and together with colistin. CAZ monotherapies were ineffective against the CAZ-resistant strain, independently of the optimized strategy and only CAZ-CI plus colistin appeared useful for clinical practice.

Insights into feasibility and microbial characterizations on simultaneous elimination of dissolved methane from anaerobic effluents and nitrate/nitrite reduction in a conventional anoxic reactor with magnetite

Discovery of nitrate/nitrite-dependent anaerobic methane oxidation (DAMO) challenges the conventional biological treatment processes, since it provides a possibility of simultaneously mitigating dissolved methane emissions from anaerobic effluents and reducing additional carbon sources for denitrification. Due to the slow growth of specialized DAMO microbes, this possibility has been just practiced with biofilms in membrane biofilm reactors or granular sludge in membrane bioreactors. In this study, simultaneous elimination of dissolved methane from anaerobic effluents and nitrate/nitrite reduction was achieved in a conventional anoxic reactor with magnetite. Calculations of electron flow balance showed that, with magnetite the eliminated dissolved methane was almost entirely used for nitrate/nitrite reduction, while without magnetite approximately 52 % of eliminated dissolved methane was converted to unknown organics. Metagenomic sequencing showed that, when dissolved methane served as an electron donor, the abundance of genes for reverse methanogenesis and denitrification dramatically increased, indicating that anaerobic oxidation of methane (AOM) coupled to nitrate/nitrite reduction occurred. Magnetite increased the abundance of genes encoding the key enzymes involved in whole reverse methanogenesis and Nir and Nor involved in denitrification, compared to that without magnetite. Analysis of microbial communities showed that, AOM coupled to nitrate/nitrite reduction was proceeded by syntrophic consortia comprised of methane oxidizers, Methanolinea and Methanobacterium, and nitrate/nitrite reducers, Armatimonadetes_gp5 and Thauera. With magnetite syntrophic consortia exchanged electrons more effectively than that without magnetite, further supporting the microbial growth.

Bioreduction of chromate in a syngas-based membrane biofilm reactor

This study leveraged synthesis gas (syngas), a renewable resource attainable through the gasification of biowaste, to achieve efficient chromate removal from water. To enhance syngas transfer efficiency, a membrane biofilm reactor (MBfR) was employed. Long-term reactor operation showed a stable and high-level chromate removal efficiency > 95%, yielding harmless Cr(III) precipitates, as visualised by scanning electron microscopy and energy dispersive X-ray analysis. Corresponding to the short hydraulic retention time of 0.25 days, a high chromate removal rate of 80 µmol/L/d was attained. In addition to chromate reduction, in situ production of volatile fatty acids (VFAs) by gas fermentation was observed. Three sets of in situ batch tests and two groups of ex situ batch tests jointly unravelled the mechanisms, showing that biological chromate reduction was primarily driven by VFAs produced from in situ syngas fermentation, whereas hydrogen originally present in the syngas played a minor role. 16 S rRNA gene amplicon sequencing has confirmed the enrichment of syngas-fermenting bacteria (such as Sporomusa), who performed in situ gas fermentation leading to the synthesis of VFAs, and organics-utilising bacteria (such as Aquitalea), who utilised VFAs to drive chromate reduction. These findings, combined with batch assays, elucidate the pathways orchestrating synergistic interactions between fermentative microbial cohorts and chromate-reducing microorganisms. The findings facilitate the development of cost-effective strategies for groundwater and drinking water remediation and present an alternative application scenario for syngas.

The construction of a microalgal-bacterial biofilm reactor for enhanced swine wastewater treatment

Traditional biological wastewater treatment methods face challenges in treating swine wastewater with a low carbon-to‑nitrogen ratio. Microalgae show great potential in low-cost swine wastewater treatment for nutrient recovery. This study investigated the effects of altering the carbon/nitrogen/phosphorus (C:N:P) ratio in swine wastewater and immobilizing microalgae for enhancing treatment using Desmodesmus sp. A C:N:P proportion of 106:16:1 was determined to be optimal for treatment. The implementation of an inclined plate bioreactor facilitated microalgal adherence, forming a biofilm that improved treatment efficiency. The biofilm exhibited removal rates of 92 % for ammonia nitrogen (NH4+-N), 98 % for total phosphorus (TP), and 99 % for chemical oxygen demand (COD). The harvested biomass from the biofilm was 7.6 times higher than that from the suspended system with a high content (63 %) of fatty acids (C16:0, C18:0, C18:1n9c and C18:2n6c) suitable for biodiesel production. Proteobacteria were dominant in the biofilm, while Firmicutes and Bacteroidetes contributed to effective organic matter and nitrogen removal. These findings present a promising technology for cost-effective swine wastewater treatment and resource recovery.

Nitrogen removal from urea effluent in an anammox sequencing batch biofilm reactor with valorised dishwashing scrubber as biocarrier

BackgroundThis study aimed for efficient nitrogen removal from urea wastewater in an anammox SBBR with valorised dishwashing scrubber as biocarrier. MethodsThe study (225 d) comprised three discrete phases: Phase I [1 – 26 d; Inf. NH4+-N (Simulated ww)], Phase II [27 – 131 d; Inf. NH4+-N (Real ww) + Inf. NH4+-N (Simulated ww)] and Phase III [132 – 225 d; Inf. NH4+-N (Real ww)] in which the reactor was supplemented with varied ratios (0 – 7) of influent NH4+-N reflecting varying combinations of real and simulated wastewater. Significant findingsThe reactor exhibited average total nitrogen removal efficiency of approximately 83 %, 81 %, and 80 % in the subsequent phases I, II, and III, respectively at varied nitrogen loading rates (100 – 232 g N m−3 d−1). The 16S rRNA gene sequencing revealed significant enrichment of Candidatus Kuenenia (from 41.69 % to 48.11 %) on the 225th day which was primarily attributed to significant decline in Nitrospira from 23.9 % to 1.24 % (due to nitrite oxidizing bacteria inhibition on account of high free ammonia concentrations in the reactor) and Nitrosomonas from 4.32 % to 0.01 %. Additionally, genera that were not initially present (on the 1st day) including Stenotrophobacter (17.34 %), Sphingobium (8.41 %), Rhodococcus (5.88%) and Ohtaekwangia (5.84 %) were observed to emerge by the end of the experiment (225 d).