The construction of a microalgal-bacterial biofilm reactor for enhanced swine wastewater treatment

Abstract

Traditional biological wastewater treatment methods face challenges in treating swine wastewater with a low carbon-to‑nitrogen ratio. Microalgae show great potential in low-cost swine wastewater treatment for nutrient recovery. This study investigated the effects of altering the carbon/nitrogen/phosphorus (C:N:P) ratio in swine wastewater and immobilizing microalgae for enhancing treatment using Desmodesmus sp. A C:N:P proportion of 106:16:1 was determined to be optimal for treatment. The implementation of an inclined plate bioreactor facilitated microalgal adherence, forming a biofilm that improved treatment efficiency. The biofilm exhibited removal rates of 92 % for ammonia nitrogen (NH4+-N), 98 % for total phosphorus (TP), and 99 % for chemical oxygen demand (COD). The harvested biomass from the biofilm was 7.6 times higher than that from the suspended system with a high content (63 %) of fatty acids (C16:0, C18:0, C18:1n9c and C18:2n6c) suitable for biodiesel production. Proteobacteria were dominant in the biofilm, while Firmicutes and Bacteroidetes contributed to effective organic matter and nitrogen removal. These findings present a promising technology for cost-effective swine wastewater treatment and resource recovery.