Biofertilizer Production Research Articles

Novel cereal bran based low-cost liquid medium for enhanced growth, multifunctional traits and shelf life of consortium biofertilizer containing Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.

The present study was carried out to valorise cereal (rice and wheat) bran for the development of low-cost liquid consortium bioformulation. Different concentrations of bran-based liquid media formulations were evaluated for the growth of consortium biofertilizer cultures (Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.). Among the bran-based formulations, wheat bran-based formulation WB5, exhibited the highest viable cell of 10.68 ± 0.09 Log10 CFU/ml and 12.63 ± 0.04 Log10 CFU/ml for Azotobacter chroococcum and Bacillus subtilis whereas for Pseudomonas sp., rice bran based bioformulation RB5 recorded maximum viability (12.71 ± 0.05 Log10 CFU/ml) after 72 h of incubation. RB51RB1: 1:10 (Rice bran: distilled water)RB2: 1:10 (Rice bran: distilled water)RB3: 1:10 (Rice bran: distilled water)RB4: 1:10 (Rice bran: distilled water)RB5: 5:10 (Rice bran: distilled water)1 and WB52WB1: 1:10 (wheat bran: distilled water)WB2: 2:10 (wheat bran: distilled water)WB3: 3:10 (wheat bran: distilled water)WB4: 4:10 (wheat bran: distilled water)WB5: 5:10 (wheat bran: distilled water)2liquid formulations were further optimized for enhanced shelf life using 5, 10 and 15 mM of trehalose, 0.05 and 0.1% carboxymethyl cellulose, and 0.5 and 1.0% glycerol. Following the peak growth at 72 h of incubation, a gradual decrease in the viable population of consortium biofertilizer cultures was observed in all the liquid formulations. The WB5 and RB5 formulations with 15 mM trehalose and 0.1% CMC, not only recorded significantly highest cell count of consortium biofertilizer cultures, but also maximally supported multi-functional traits i.e., phosphate and zinc solubilization, ammonia and IAA production up to 150 days. Further evaluation of seedling emergence and growth of wheat (PBW 826) under axenic conditions recorded WB5 amended with 15 mM trehalose-based consortium bioformulation to exhibit maximum emergence and growth of wheat seedlings. This low-cost liquid formulation can be used for large-scale biofertilizer production as a cost-effective liquid biofertilizer production technology.

Optimizing the process of sewage sludge collection for bioenergy generation and biofertilizer production

The conversion of sludge from sewage treatment plants (STPs) into bioenergy is a promising approach to sustainable energy generation and effective waste management. This process occurs through anaerobic digestion in the biodigesters, resulting in biogas production. However, given that the sludge comes from various STPs in different cities, identifying the ideal locations requires careful selection due to the complexity of sludge management, associated costs, and transportation logistics. In this context, this work aims to formulate a mathematical optimization model to determine the best locations for the installation of sludge processing centers, considering the minimization of the economic, environmental, and social costs. The model is implemented in MATLAB software, and geospatial analysis of the regions of interest is performed using QGIS software. For model validation, three distinct instances are considered. The results obtained demonstrate significant potential in terms of biogas and electricity production, with the highest values for the third instance being 61,997,501 Nm³/year and 149,275 MWh/year, respectively. The proposed methodology represents a crucial step toward sustainability, as it promotes the generation and use of bioenergy from STP sludge and contributes to improved waste management and the prevention of air, soil, and water pollution.

ENVIRONMENTAL SAFETY OF THE TECHNOLOGY OF HANDLING DIGESTATE OF DIFFERENT GENESIS

In the modern world, biogas technology is gaining momentum as an ecological alternative to the production of biofertilizer after anaerobic fermentation of various types of waste. Due to the large amount of organic, nutrient and biologically active substances, biogas digestates are often applied directly to the soil as an organic fertilizer to improve soil quality and increase yield. The purpose of the article is to determine the ecological safety of the digestate obtained after anaerobic fermentation of chicken litter and sewage sludge, as part of a biocomposite for soil purification from heavy metals. The methodological basis of this study are experiments on the phytotoxicity of digestate samples based on the results of the germination of pepper seeds in Petri dishes using aqueous solutions of untreated digestate and digestate treated with phosphogypsum. The expediency of choosing a phosphogypsum treatment method was due not only to the possibility of reducing the presence of pathogens in digestates, but also to obtain a biocomposite capable of adsorbing harmful elements from the soil and feeding it with necessary substances. Using the germination index, the level of phytotoxicity of digestate based on various organic wastes was assessed, the effectiveness of the use of digestate as a biological fertilizer was substantiated. It was determined that digestate based on sewage sludge became an ecologically safe substrate for the germination of pepper seeds. Digestate from chicken litter treated with phosphogypsum on the 20th day of anaerobic fermentation showed a phytotoxic effect on pepper seeds. Indicators of the germination index increased on the 30th day of anaerobic fermentation, which indicates a decrease in the toxicity of the material after the end of the anaerobic fermentation process. In general, a germination index higher than 50% was observed in all samples of untreated digestate and a greater number of samples of treated digestate for both substrates. The article provides recommendations for evaluating the maturity of digestate using the seed germination index as a quick indicator for determining the phytotoxicity of substrates and, accordingly, the level of environmental safety of the material for soil and plants.

Environmental Assessment of Pig Manure Treatment Systems through Life Cycle Assessment: A Mini-Review

The primary aim of this research was to evaluate and compare the environmental impacts, throughout the life cycle, of the main treatment systems employed by the industry, as well as to identify the processes that contribute most to these environmental impacts. To achieve this, a bibliographical search was conducted using the Web of Science Core Collection database platform, utilizing the keywords “life cycle assessment”, “pig”, “treatment”, and “manure” or “slurry”. The search was restricted to publications from the last five years (2019–2023), resulting in a total of 66 publications that were then analyzed according to the functional unit (FU) adopted. For the 10 publications whose FUs were expressed in tons or cubic meters of treated manure, a descriptive and quantitative analysis was carried out. It was found that anaerobic digestion has been the most widely used treatment technology for pig manure over the past five years, according to the LCA methodology. These systems, configured as biogas and biofertilizer production facilities, have proven to be environmentally friendly and could play a crucial role in the energy transition and decarbonization of the energy matrix.

NPK Biofertilizer Production from Banana Peel, Feather and Bone Ashes and their Comparative Advantage to 20:10:10 Inorganic Fertilizer

The possibility of formulating nitrogen, phosphorus and potassium (NPK) biofertilizer from ashes of waste organic materials such as chicken feathers, bone and banana peels is investigated in this study by first subjecting the agricultural waste to 80-250 heat in a muffle furnace to produce ash. Kjeldatherm block digestion unit, UV spectrophotometer, and flame photometers, respectively helped in measuring the concentrations of N, P and K inherent in the fertilizer precursors as well as in 5 formulated blends (i.e., A, B, C, D & E). It was discovered that NPK in the single substrate and blends favorably compared with standard NPK 20:10:10 chemical fertilizer to some extent. Among single material fertilizer sources, feather ash with 0.179:1:0.134 NPK and bone ash with 0.009:1:0.021 NPK had the closest nutrient content with the standard, showing potential promise. On the other hand, Blend E is the same as the standard, followed by Blend A, B, D and C, which are hierarchically close in elemental composition to the standard. The choice of these biofertilizers is dependent on their nutrient compositions, the type of crops to be grown, and the soil mineral requirements. Most importantly, different NPK ratio organic fertilizers produced in this study can competitively be produced in a large scale to address huge costs associated with the NPK 20:10:10 standard commercial fertilizer. Blend E (NPK 20:10:10) can be formulated locally by farmers in rural areas easily using this particular agricultural residue or a host of other confirmed farm wastes.

Important soil microbiota's effects on plants and soils: a comprehensive 30-year systematic literature review.

Clarifying the relationship between soil microorganisms and the plant-soil system is crucial for encouraging the sustainable development of ecosystems, as soil microorganisms serve a variety of functional roles in the plant-soil system. In this work, the influence mechanisms of significant soil microbial groups on the plant-soil system and their applications in environmental remediation over the previous 30 years were reviewed using a systematic literature review (SLR) methodology. The findings demonstrated that: (1) There has been a general upward trend in the number of publications on significant microorganisms, including bacteria, fungi, and archaea. (2) Bacteria and fungi influence soil development and plant growth through organic matter decomposition, nitrogen, phosphorus, and potassium element dissolution, symbiotic relationships, plant growth hormone production, pathogen inhibition, and plant resistance induction. Archaea aid in the growth of plants by breaking down low-molecular-weight organic matter, participating in element cycles, producing plant growth hormones, and suppressing infections. (3) Microorganism principles are utilized in soil remediation, biofertilizer production, denitrification, and phosphorus removal, effectively reducing environmental pollution, preventing soil pathogen invasion, protecting vegetation health, and promoting plant growth. The three important microbial groups collectively regulate the plant-soil ecosystem and help maintain its relative stability. This work systematically summarizes the principles of important microbial groups influence plant-soil systems, providing a theoretical reference for how to control soil microbes in order to restore damaged ecosystems and enhance ecosystem resilience in the future.

Production of a Rich Fertilizer Base for Plants from Waste Organic Residues by Microbial Formulation Technology.

This review explores different methods of sustainably introducing nutrients from agro-industrial waste into the soil. The focus is on sustainable agriculture and how the soil system can be modified by introducing secondary raw materials and beneficial microorganisms. Soil is a nexus between plants and microorganisms that must be balanced. The article emphasizes the importance of maintaining the microbiological balance when supplying nutrients. This review is focused on the possible techniques involved in the production of biofertilizers and their mode of application into the soil system and on plants. We addressed several advantages concerning the use of beneficial microorganisms in waste management by microbial formulation techniques. Not only the advantages but several limitations and challenges were also discussed in regard to the large scale production of microbial products. Therefore, the proper treatment of industrial waste is essential so that we can preserve the environment and human safety and also achieve sustainable agriculture.

Genomic insights and anti-phytopathogenic potential of siderophore metabolome of endolithic Nocardia mangyaensis NH1

Actinobacteria are one of the predominant groups that successfully colonize and survive in various aquatic, terrestrial and rhizhospheric ecosystems. Among actinobacteria, Nocardia is one of the most important agricultural and industrial bacteria. Screening and isolation of Nocardia related bacteria from extreme habitats such as endolithic environments are beneficial for practical applications in agricultural and environmental biotechnology. In this work, bioinformatics analysis revealed that a novel strain Nocardia mangyaensis NH1 has the capacity to produce structurally varied bioactive compounds, which encoded by non-ribosomal peptide synthases (NRPS), polyketide synthase (PKS), and post-translationally modified peptides (RiPPs). Among NRPS, five gene clusters have a sequence homology with clusters encoding for siderophore synthesis. We also show that N. mangyaensis NH1 accumulates both catechol- and hydroxamate-type siderophores simultaneously under iron-deficient conditions. Untargeted LC–MS/MS analysis revealed a variety of metabolites, including siderophores, lipopeptides, cyclic peptides, and indole-3-acetic acid (IAA) in the culture medium of N. mangyaensis NH1 grown under iron deficiency. We demonstrate that four CAS (chrome azurol S)-positive fractions display variable affinity to metals, with a high Fe3+ chelating capability. Additionally, three of these fractions exhibit antioxidant activity. A combination of iron scavenging metabolites produced by N. mangyaensis NH1 showed antifungal activity against several plant pathogenic fungi. We have shown that the pure culture of N. mangyaensis NH1 and its metabolites have no adverse impact on Arabidopsis seedlings. The ability of N. mangyaensis NH1 to produce siderophores with antifungal, metal-chelating, and antioxidant properties, when supplemented with phytohormones, has the potential to improve the release of macro- and micronutrients, increase soil fertility, promote plant growth and development, and enable the production of biofertilizers across diverse soil systems.

Auckland Council Food Scraps Project

This research project delves into the complexities of Auckland City Council's food scraps collection rollout, spanning from kerbside collection to the Anaerobic Digestion Facility in Reporoa, Rotorua; where collected food scraps are transformed into environmentally friendly fertilisers and renewable gases. The project comprises two key components: assisting in the creation of a dashboard to display weekly and monthly collection and disposal data to monitor ongoing participation rates and collected tonnage requirements are met; and assisting with the continuation of the food scraps rollout initiative in wider Auckland regions and help increase consumer knowledge and involvement. This is in conjunction of aligning with Auckland Council's aim for zero waste by 2040. The initiative aims to keep food scraps out of landfills, minimising harmful greenhouse gas emissions and return valuable nutrients to the land through biofertiliser production. This project required me to learn about Auckland's circular economy solution which emphasises prevention of food waste, supporting redistribution initiatives, and promoting home and community composting. As part of helping with the rollout and helping create an easy way for residents to separate their food scraps from general rubbish, individuals have gained a raised awareness of wastage, leading to considered meal planning and cost savings. The government's commitment to nationwide food scraps services by 2030 underscores the urgency of sustainable waste management practices which it why it was crucial for me to be familiar with the whole process and sectors within the rollout to assist Auckland Council to meet this commitment. This project has also meant that I was able to understand the whole process of the Anaerobic Digestion Facility's innovative technology which processes organic material into biogas and nutrient-liquid fertiliser, contributing to New Zealand's sustainability goals. Through comprehensive monitoring and filtration systems, the facilities uphold environmental standards while pioneering large-scale food scraps processing in the country.
 Supervised by: Sarah Nicholls
 Scholarship Project Funded by: Auckland City Council

Food waste-based bio-fertilizers production by bio-based fermenters and their potential impact on the environment

Increasing food waste is creating a global waste (and management) crisis. Globally, ∼1.6 billion tons of food is wasted annually, worth ∼$1.2 trillion. By reducing this waste or by turning it into valuable products, numerous economic advantages can be realized, including improved food security, lower production costs, biodegradable products, environmental sustainability, and cleaner solutions to the growing world's waste and garbage management. The appropriate handling of these detrimental materials can significantly reduce the risks to human health. Food waste is available in biodegradable forms and, with the potential to speed up microbial metabolism effectively, has immense potential in improving bio-based fertilizer generation. Synthetic inorganic fertilizers severely affect human health, the environment, and soil fertility, thus requiring immediate consideration. To address these problems, agricultural farming is moving towards manufacturing bio-based fertilizers via utilizing natural bioresources. Food waste-based bio-fertilizers could help increase yields, nutrients, and organic matter and mitigate synthetic fertilizers' adverse effects. These are presented and discussed in the review.

Increasing Concentrations of Arthrospira maxima Sonicated Biomass Yields Enhanced Growth in Basil (Ocimum basilicum, Lamiaceae) Seedlings

The continued increase in human populations and use of chemical fertilizers remain a threat to the health and stability of human–ecological systems worldwide. To ameliorate this problem and achieve long-term food security, a variety of ecofriendly technologies have been developed, including the production of cyanobacteria-based biofertilizers. This technology can be optimized through experiments that assess how plant growth is enhanced under different biofertilizer concentrations (g L−1). In this study, the biofertilizer capabilities of various concentrations of sonicated biomass (0, 2.5, 5, 10, 20, and 40 g L−1) derived from the cyanobacteria Arthrospira maxima on the growth of basil (Ocimum basilicum, Lamiaceae) were assessed, comparing their effectiveness with that of a positive control, a commercial biofertilizer (OptiMar Algas Marinas®) administered at 4 mL L−1. Generally, increased concentrations led to enhanced growth parameters; however, discernible differences from the negative control (0 g L−1) were often observed only when concentrations exceeded 5 g L−1. Surprisingly, the negative and positive controls often yielded similar results. A chemical composition analysis of A. maxima revealed high concentrations of the phytohormones, macronutrients, and essential amino acids that likely explain how our A. maxima sample enhanced growth in basil. Further research is required to determine how other crop plants respond to different concentrations of A. maxima. Additionally, assessing the feasibility of creating an economically accessible product with a higher concentration of A. maxima is crucial for practical applications.

Conventional and Advanced Wastewater Treatment Techniques; Development of Sustainable Environment and Production of Biofertilizers

Conventional and Advanced Wastewater Treatment Techniques; Development of Sustainable Environment and Production of Biofertilizers

MICROALGAS: UMA ESTRATÉGIA SUSTENTÁVEL NA TRANSFORMAÇÃO E OBTENÇÃO DE COMPOSTOS ORGÂNICOS

MICROALGAE: A SUSTAINABLE STRATEGY IN THE TRANSFORMATION AND OBTAINING OF ORGANIC COMPOUNDS. The present review presents the biotechnological potential of microalgae, highlighting its applications in biofuels, bioremediation, production of bioactive compounds, biocatalysts, and biofertilizers. Microalgae are photosynthetic microorganisms that combine characteristics of plants and heterotrophic microorganisms, such as yeasts, bacteria, and fungi. They can be considered biotechnological agents of extreme versatility and metabolic adaptability. Its photosynthetic efficiency can absorb large volumes of anthropogenic CO2 and convert it into biomass rich in compounds of high biological and energetic value, enabling the development of sustainable bioremediation processes and the production of third-generation biofuels and biofertilizers. In addition, its biomass has aroused interest in obtaining compounds with biological action, such as carotenoids, mycosporine-like amino acids, polyunsaturated fatty acids, sterols, phycocolloids, carbohydrates, and proteins. The biotechnological potential of microalgae does not end with the production and extraction of its biomass. Its dynamic and adaptive metabolism allows its use in the biotransformation of xenobiotic substrates and the production of compounds of chemical and pharmaceutical interest. In this perspective, microalgae have application qualities as a biotechnological platform in developing synergistic processes between the environment, society, and industry, thus contributing to the expansion and use of Green Chemistry and strengthening the circular economy.

Biofertilizers production using plant growth-promoting bacteria from cassava peels and plantain leaves to evaluate the effects on growth parameters of beans (Phaseolus vulgaris L.) and Groundnut (Arachis hypogaea L.) seeds

Biofertilizers production using plant growth-promoting bacteria from cassava peels and plantain leaves to evaluate the effects on growth parameters of beans (Phaseolus vulgaris L.) and Groundnut (Arachis hypogaea L.) seeds

Biofertilizers Production and Climate Changes on Environmental Prospective Applications for some Nanoparticles Produced from some Microbial Isolates

Biofertilizers Production and Climate Changes on Environmental Prospective Applications for some Nanoparticles Produced from some Microbial Isolates

ТЕОРЕТИЧЕСКИЕ АСПЕКТЫ ПРОЦЕССА ПОЛУЧЕНИЯ БИОГАЗА ПРИ АНАЭРОБНОЙ ФЕРМЕНТАЦИИ ОРГАНИЧЕСКИХ ОТХОДОВ

The biogas extraction process's theoretical description during carbon containing agricultural waste's anaerobic digestion is considered. This proposed installation's work for biogas and organic fertilizers production during waste's anaerobic digestion is described. These anaerobic methods' advantage as biological treatment's unlike classical aerobic methods, when all bacteria work under the same conditions, with properly organized anaerobic processes is made, the separated bacteria in different physico-chemical conditions working. This makes it possible the anaerobic digestion process in productivity terms optimizing, managing and, ultimately, the waste processing quality improving. Based on the proposed biogas installation, the equations for the biogas yield determining are obtained. The of pig manure biogas output's theoretical results with crushed corn grain's addition are presented. This biogas installation's calculation method on the biogas output in dependence on empirical and technical parameters' design is based. The aim is biogas and biofertilizer's production during pig manure fermentation intensity increasing. The proposed biogas installation in psychophilic (3-25°C), mesophilic (25-40°C) and thermophilic (40-55°C) temperature conditions can operating. The substrate is heated by biogas through a vortex tube passing. The organic substances' anaerobic processing leads the biodegradable substances' main part into CO2, H2O and NH3 decompositing. Bacteria such as Bacillus, Micrococcus, Pseudomonas and Clostridium play a key role in this process by enzymatic hydrolysis and acid's formation carrying out. The theoretical calculations show that the highest biogas producing with a content of solid particles in the fertilizer of no more than 8,8-10% and substrate's effective mixing is achieved. The organic waste processing into biogas is justify and preferable. This product (biogas) everywhere can be used, both on individual agricultural firms and in general in agriculture, where it will be the most effective using.

Improved agro-industrial waste utilization in biogas and fertilizer production ensuring CO2 sequestration

The global food crisis approach encourages finding new sources of replenishment of the phosphorus deficit in agriculture, making the unconventional sources of phosphorus particularly relevant. Despite numerous studies of phosphorus recovery methods, the need to increase the content of nutrients in the organo-mineral fertilizers obtained via anaerobic digestion of sewage activated sludge from municipal wastewater still exists. The greatest success in this area has been achieved by the biotechnologies which ensure the simultaneous production of biogas and biofertilizers. The present study proposes a sustainable technology for anaerobic digestion with the simultaneous capture of carbon dioxide and sodium carbonate production. It involves pre-comminution of the organo-mineral substrate (vegetal waste, activated sludge, phosphorus-containing component, and cheese whey) facilitating anaerobic digestion, and extraction of CO2 from biogas by absorption with a 10 % NaOH solution. Biomethanation reaction was found to have the first order with the activation energy of 24.8 kJ/mol, whereas the carbon dioxide absorption degree is proportional to the CO2 content in biogas. The results showed good potential for increasing the efficiency of both phosphorus recovery from municipal wastewater and anaerobic digestion to produce valuable products, including high-purity bio-methane (96 vol. % CH4), concentrated (∑NPKCa 53 %) organo-mineral fertilizer from digestate and soda ash. Obtained fertilizer increased potato yield by 35 wt. %. The study demonstrates a novel concept of carbon dioxide cycle looping in biogas production, as well as reducing greenhouse gas emissions by converting up to 98.7 % of CO2 into an additional marketable product – sodium carbonate, which contains 99 % Na2CO3.

Cyanobacteria and microalgae as potential sources of biofertilizers: a review

Cyanobacteria and microalgae represent promising sources for sustainable production of biofertilizers and biostimulants, which can improve crop yield and quality and contribute to food security. However, despite their potential, their exploration remains incomplete, hindered by technical and economic challenges that arise when attempting to scale up production. The primary focus of this review is to delve into the active chemical compounds responsible for the biofertilizing and biostimulating roles of cyanobacteria and microalgae. In addition, it explores the essential unit operations involved in transforming their biomass into potential bioproducts. Moreover, this review highlights studies that have employed cyanobacteria and microalgae as sources of biofertilizer in various crops, describing their mode of action and application. By integrating cyanobacteria and microalgae processing with other advanced biotechnological, the viability of these products for sustainable agriculture can be significantly enhanced.

An Assessment of the Elemental Compositions of Neem (Azadirachta indica A. Juss) Products for Biofertilizer Production

The use of soil-nutrients enhancers is crucial to crop and vegetable farmers. However, with increasing costs of mineral fertilizers, seeking alternatives in the form of biofertilizers may help to alleviate farmers concerns. Neem (Azadirachta indica A. Juss) products - seeds, tree bark, and leaves - have been documented in the literature to have soil enriching properties. This study examines the elemental compositions of neem tree bark, leaves, and mixture (leaves and bark) to suggest the most suitable for biofertilizer production. Fresh neem leaves (young and old leaves were discarded) and tree bark (harvested 0.4 – 1.70 m above ground) were harvested. chopped into bits, air-dried under the shade for 7 days, milled into powder using a mortar and pestle, sieved (with a 1.7 mm sieve) and packed into air tight nylon bags for moisture content and elemental composition determinations. The relevant test methods were used to determine the elemental compositions of the samples of neem leaves, tree bark, and mixture of bark and leaves. The results revealed that there was no statistically significant difference between the samples at p>0.05.This suggests that any of the samples can serve as a good source for biofertilizer production. Since neem leaves had the highest nutritional elements by composition and relatively easier to obtain, this suggests that neem leaves can be recommended as a green fertilizer to support crop and vegetable growth. However, its use as a straight fertilizer may require further investigations.

Exploring the potential of sugarcane vinasse for biogas and biofertilizer Production: A catalyst for advancing the bioeconomy

The present research looks into the possibilities of biogas obtained from anaerobic fermentation of biogas vinasse and how it may be used in a variety of applications. The utilization of vinasse biogas (VBG) for electricity production is investigated, and its cost and energy potential are described. Although vinasse is a harmful pollutant if released into the environment without proper treatment, it may be of use as a source of water and nutrients if the right ingredients are added. Yet, the high price of purification has cast doubt on its practicality. Bioproduct and vinasse management developments and trends were examined. Several studies point towards the effective utilization of VBG for producing biofuels, but it's also important to consider that agriculture was the starting point of the sucrose-alcohol cycle. Vinasse is rich in nutrients and minerals, making it an ideal fertilizer. The present study seeks to provide a paradigm in which expensive and import-dependent power plants are complemented by auxiliary biogas facilities, resulting in overall advantages. The study also shows that significantly more energy may be generated and that higher volumes of fossil-based fuels may be replaced through efficient employment of vinasse. The study offers fresh insights into how to facilitate the evolution of a low-carbon society by integrating vinasse biogas capability with specific conventional fuel utilization.