In order to produce a clean and trouble-free biodiesel that will represent true alternative to fossil diesel, this work evaluates the performance of a multi feedstock plant for biodiesel production. The performance evaluation of the developed plant was conducted using used frying oil and palm olein oil using acid and alkali transesterification process respectively. Prior to that, the feedstock characterization in terms of moisture content and the amount of free fatty acid in the used and unused oils were determined. The biodiesels produced were characterized and its properties compared in respect of the American Society for Testing and Materials (ASTM D6751) and EN 14214 limits for biodiesel. Also, emissions of some pollutants from the diesel engine were measured at 2000 rpm using the AUTOplus gas analyzer for diesel and blends of biodiesel. Results obtained indicates that the developed plant can transesterify used and unused vegetable oil to biodiesel and can also undertake both alkali-catalysed and acid-catalysed transesterification.