Abstract
During biodiesel storage, chemical reactions may occur, producing sludges. The aim of this study was to characterize the chemical and microbial composition of the sludge found in a biodiesel storage tank. The material was collected in a biodiesel production plant. The sludge chemical characterization was performed by Fourier transform infrared (FTIR) spectroscopy, flame atomic absorption spectroscopy (FAAS), gas chromatography (GC), and the microbial investigation used culture-dependent techniques. The deteriogenic potential of the native microbial community was evaluated using the sludge as a microbial inoculum in a 60 day experiment. The microbial growth, biodiesel degradation, pH alterations, and the detection of esters in the aqueous phase were evaluated. The chemical analysis indicated the sludge composition as fatty acids esters and metallic ions; sterols glycosides were not detected. Seven bacteria and five fungi species were obtained from the sludge. The microbial growth analysis indicated that the native community does not have high biodiesel deteriogenic capacity.
Highlights
The use of biofuels derived from renewable raw materials has been increasingly common in the global energy field
According to these results of the solid phase chemical analysis, the sludge collected was predominantly composed by fatty acid esters
Our experiments demonstrated that the native microbial community from the sludge was not capable to grow under the conditions established, the lack of biomass detected in this treatment may indicate that the microbial population was low in density
Summary
The use of biofuels derived from renewable raw materials has been increasingly common in the global energy field. The national production of biodiesel occurs mainly by transesterification reaction using methanol.[2] The Brazilian National Agency of Petroleum, Natural Gas, and Biofuels (Agência Nacional do Petróleo, Gás Natural e Biocombustíveis, ANP) Resolution No 45/20143 (henceforward called ANP 45) describes the quality requirements for commercialized biodiesel. Because it is commonly composed of fatty acid methyl esters (FAMEs), biodiesel has a higher degradability than diesel. The ANP 45 determines the acceptable limit of concentrations of these substances in biodiesel.[3] In addition, sterols glycosides from the
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