ABSTRACT For future construction of implantable tissue structures, the development of 3-dimensional fluidic microsystems is necessary. In the current work we have developed a unique technology for 3-dimensional microfluidic environments with potential for tissue engineering applications. The microfluidic devices were fabricated out of biodegradable and biocompatible elastomers, such as poly (glycerol-sebacate) and poly (erythritol-sebacate), due to their transparency and thermoset properties suitable for microfabrication technologies. The three-dimensionality of the microfluidic bioreactors was imparted by a porous poly L-lactic acid membrane prepared via electrospinning. Thus obtained membrane has a high surface area-to-volume ratio and is intended to mimic the extracellular matrix that provides physical support and spatial organization. The membrane was cut and placed in between two micro-patterned microfluidic layers during the alignment process, and subsequently bonded to achieve the final bioreactor. The patency of the device network was evaluated and the bond between the microfluidic layers was sufficiently strong to allow perfusion flow rates in the range 0.1–300 μl/min. The herein developed technology comes as solution to the two-dimensionality issue that exists in most microfluidic devices and can relate to a variety of tissue engineering applications.