Biochemical Reactions Research Articles

Investigation of biochemical and antioxidant system responses of sugarcane CP48-103 under drought stress induced by polyethylene glycol

Investigation of biochemical and antioxidant system responses of sugarcane CP48-103 under drought stress induced by polyethylene glycol

Characterization of 2-phenanthroate:CoA ligase from the sulfate-reducing, phenanthrene-degrading enrichment culture TRIP.

Polycyclic aromatic hydrocarbons (PAHs) constitute a class of very toxic and persistent pollutants in the environment. However, the anaerobic degradation of three-ring PAHs such as phenanthrene is barely investigated. The initial degradation step starts with a carboxylation followed by a CoA‑thioesterification reaction performed by an aryl-CoA ligase. The formation of a CoA-thioester is an important step in the degradation pathway of aromatic compounds because the CoA-ester is needed for all downstream biochemical reactions in the pathway. Furthermore, we provide biochemical proof for the identification of the first genes for anaerobic phenanthrene degradation. Results presented here provide information about the biochemical and structural properties of the purified 2‑phenanthroate:CoA ligase and expand our knowledge of aryl-CoA ligases.

Purcell gain equalized zero-mode waveguide

We refresh the design of zero-mode waveguides (ZMWs) by introducing metamaterials that makes the zeroth order resonant mode existence. Of particular importance, the resulting electromagnetic field exhibits nearly constant distribution but not a trivial solution of Maxwell’s equation, showing great advantage to equalize the excitation rate of molecules throughout the waveguides. A closed form expression for the wave impedance is derived which is verified by the finite-difference time-domain simulations. Benefitted from the cavity Purcell effect which is lacking in existing ZMWs, fluorescence amplification and lifetime reduction are simultaneously enhanced. A practical design where the excitation volume reduced down to sub-zeptoliter and the fluorescence lifetime shortened to picosecond scale is illustrated. This result makes single molecule real time (SMRT) sensing of biochemical reactions at biophysically relevant concentrations (~ μM) possible, combining off-the-shelf ultrafast lasers.

Monitoring the Dilution of Buffer Solutions with Different pH Values above and below Physiological pH in Very Small Volumes.

The accurate determination of the post-dilution concentration of biological buffers is essential for retaining the necessary properties and effectiveness of the buffer to maintain stable cellular environments and optimal conditions for biochemical reactions. In this work, we introduce a silicon-based impedance chip, which offers a rapid and reagent-free approach for monitoring the buffer concentrations after dilution with deionized (DI) water. The impedance of the impedance chip is measured, and the impedance data are modeled using a multiparameter equivalent circuit model. We investigated six aqueous biological buffers with pH values above and below the physiological pH for most tissues (pH ~ 7.2-7.4) following dilution with DI water by factors of 2.0, 10.0, 20.0, 100.0, and 200.0. The impedance measurement is then performed for the frequency spectrum of 40 Hz to 1 MHz. From the interpretation of the impedance measurement using the multiparameter equivalent circuit model, we report a buffer-sensitive equivalent circuit parameter RAu/Si of the silicon-based impedance chip showing a linear trend on a logarithmic scale with the buffer concentration change after dilution. The parameter RAu/Si is independent of the buffer pH and the added volume. The results demonstrate the efficacy of the silicon-based impedance chip as a versatile tool for precise post-dilution concentration determination of diverse biologically relevant buffers. The presented impedance chip offers rapid, accurate, and reliable monitoring, making it highly suitable for integration into automated liquid-handling systems to enhance the efficiency and precision of biological and chemical processes.

Freezing Functional Nucleic Acids: From Molecular Reactions to Surface Immobilization.

Biochemical reactions are typically slowed down by decreasing temperature. However, accelerated reaction kinetics have been observed for a long time. More recent examples have highlighted the unique role of freezing in fabricating supermaterials, degrading environmental contaminants, and accelerating bioreactions. Functional nucleic acids are DNA or RNA oligonucleotides with versatile properties, including target recognition, catalysis, and molecular co4mputing. In this review, we discuss the current observations and understanding of freezing-facilitated reactions involving functional nucleic acids. Molecular reactions such as ligation/conjugation, cleavage, and hybridization are discussed. Moreover, freezing-induced DNA-nanoparticle conjugations are introduced. Then, we describe our effect in immobilizing DNA on bulk surfaces. Finally, we address some critical questions and research opportunities in the field.

URINARY TRACT INFECTIONS AND TOTAL PROSTATE SPECIFIC ANTIGEN TPSA

Introduction: The urinary system consists of four main organs: the kidneys, ureters, bladder, and urethra, and these organs work to filter blood, remove waste, form urine, and eliminate urine from the body. In recent years there have been encouraging strides in understanding and studying the pathogenesis of urinary tract infections (UTIs) and total prostate specific antigen-TPSA. Purpose of the study: The purpose of the research will focus on biochemical markers such as: urea, creatinine, uric acid as urine degradation products and identification of parameters of diseases of the urogenital tract. Urinary status parameters will also be analyzed such as: proteinuria, hematuria, glycosuria, bilirubin, urobilinogen, pH, etc., parameters which are present during diseases and infections of the urogenital tract. The purpose of the paper will also analyze the bacteriological and virological parameters such as: ureoplasma, mycoplasma, chlamydia, toxoplasma and cytomegalovirus, which are identifying parameters for infections of the urogenital tract in different age groups and genders. This work will also consist in the measurement of total prostate specific antigen-TPSA as a main marker for the identification of prostate diseases. Material and method: In the research, a total of 50 male patients will be taken to analyze the urinary condition. Urine analyzes will be performed by collecting urine in sterile cups and using the urine microscopy method and the urine strip test using the chromatographic method, where these analyzes were performed according to the European guideline standards manual (2023). Vitek is an identification system that can identify bacteria and yeast. This test uses the biochemical reactions and nutrient utilization of the microorganism to make the identification of the bacteria and the antibiogram. The test requires that a sufficient amount of growth be obtained during a specified growth period of 18 – 70 hours. Patients of the study group for Total Prostate Specific Antigen (TPSA) were analyzed through the blood serum in ng/mL of the patients with the fluorescent immunoassay test method with Vidas Biomerie. Also 20 patients to analyze the total prostate specific antigen TPSA with serum and will be analyzed with fluorescent enzymatic immunoassay methods (Vidas). SPSS – Software package version 20.0 Numerical series were analyzed using central tendency measures (average, median, minimum values, maximum values), as well as distribution measures (standard deviation); To determine the regularity of the frequency distribution of the examined variables, the Shapiro-Wilk W Test was used; Non-parametric tests for two independent parameters were used to test the significance and difference between some numerical parameters with irregular frequency distribution (Mann Whitney U test); To determine statistical significance, a two-way analysis was used with a significance level (means) of p<0.05. Results: From the results, it was found that urinary infections are frequent and appear during the attack with bacteria and viruses such as ureoplasma, mycoplasma, chlamydia and cytomegaloviruses, which are identifying parameters for infections of the urogenital tract in different age groups of the male gender. Conclusion: From the analyzes performed on patients of different age groups, we came to the conclusion that: • The parameters of the urinary system will be able to give a real picture of infectious diseases of the urinary tract. • Biochemical markers can identify the functional state of the urinary tract. • Antibiogram and cytomegaloviruses - microbiological and virological status can give an insight into the type of urinary infections. • The prostate-specific antigen TPSA can be taken as the main marker for the identification of tumoral disease of the prostate gland.

Identification of Microbiota Causing Bromhidrosis in the Axillary

Bromhidrosis is a clinical condition defined by an uncomfortable or unpleasant body odor. Since the apocrine glands are only active during puberty, most bromhidrosis occurs in the apocrine glands in the armpit and occurs when apocrine gland secretions approach the skin's surface, where they undergo various biochemical reactions caused by the presence of microbiota in the axillary skin, resulting in odor in the axilla. This research aims to identify microbiota species in the axilla that can cause bromhidrosis. A laboratory experimental research design with purposive sampling is used in this study. In this study, 60 samples were collected from students aged 15 to 18 years old at a high school in Medan, and the samples were analyzed at the University of North Sumatra Hospital's Microbiology Laboratory. All samples were taken from the right axillary skin swab, a Gram stain was performed, and the identified microbiota isolates were identified. The results of skin smear culture revealed a Gram-positive cocci group in 59 samples (98.3%) and a Gram-positive bacilli group in only one sample (1.7%). The microbiota responsible for bromhidrosis was identified as Staphylococcus hominis spp. hominins (30.4%), Staphylococcus epidermidis (27.8%), Staphylococcus aureus (16.5%), Kocuria kristinae and Staphylococcus haemolyticus (6.3%), Staphylococcus lugdunensis and Staphylococcus saprophyticus. The most common microbiota that causes bromhidrosis in the axilla is Staphylococcis hominis spp. Hominis, Staphylococcus epidermidis, and Staphylococcus aureus.

Pirfenidone targeted mechanisms for alleviating methotrexate-induced testiculopathy in Wistar rats.

Testicular injury and affected spermatogenesis are major complications of methotrexate (MTX) use. Oxidative stress is one contributing process leading to inflammation and apoptosis induction. Pirfenidone (PFD) is a well-known anti-fibrotic drug prescribed for interstitial lung fibrosis, in addition to anti-inflammatory, antioxidative, and antiapoptotic capabilities. The study aimed to explore the potential protection afforded by PFD in a rat model of MTX-induced testiculopathy. The experimental design included four groups, each containing seven adult Wistar rats: control, PFD (500mg/kg/day, orally)-, MTX (0.5mg/kg, intraperitoneal, twice weekly)-, and PFD/MTX-treated groups. Treatment continued for 4weeks. Blood and testicular samples were harvested for biochemical, histological, immunohistochemical, and polymerase chain reaction (PCR) analyses. Also, the testicular damage and spermatogenic activity were graded by the testicular injury and Johnsen scoring system, respectively. PFD positively affected the serum testosterone (TST) level, reduced the testicular inflammatory mediators [tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β)], reduced the testicular oxidative burden, increased superoxide dismutase (SOD), and protected the testicular histological structure. In addition, antifibrotic effects, anti-caspase-3, and PCNA enhancement activity were recorded. PFD exhibited a protective potential and mitigated the MTX-induced testiculopathy via suppression of testicular oxidative stress, inflammation, fibrosis, and apoptosis and retaining the testicular proliferative efficacy as confirmed by histological, immunohistochemical, and biochemical methods.

ВПЛИВ МІКОТОКСИНУ Т-2 НА ІХТІОЛОГІЧНІ ПОКАЗНИКИ КОРОПОВИХ РИБ

Mycotoxins are particularly dangerous for both animals and humans. Their toxic effects manifest even in ultra-minimal doses, which are often impossible to detect even with modern methods. The response mechanism of hydrobionts to toxic pollution in the aquatic environment is revealed through biochemical and physiological reactions aimed at restoring damaged functions. Increased human impact on aquatic ecosystems has exacerbated the survival challenges for aquatic organisms under stressful conditions. A study was conducted to investigate the impact of mycotoxin T-2 on ichthyological indicators in carp fish. Even ultra-low doses of mycotoxins can have toxic effects, and their presence is often difficult to detect with modern methods. Changes in morphometric indicators and metabolic processes are integral components of complex nonspecific reactions that occur in response to any stress factors. During long-term exposure or at high intensity, serious and irreversible damage can occur, potentially leading to various pathologies or even death of the organism. Based on this, the aim of the study was to examine the effect of mycotoxin T-2 on the ichthyological indicators of carp fish. A laboratory experiment showed that exposure to mycotoxin T-2 led to a slight increase in the fatness ratio of fish, which could be explained by tissue swelling. A similar trend was observed regarding the liver index in carp. In the case of crucians, the liver index remained unchanged. Additionally, no significant changes were found in the indicators of body height, head height, and compactness. This can be attributed to the relatively short two-week experimental period, which may not be sufficient to detect differences in morphological parameters. During the experiment, when mycotoxin T-2 was introduced into laboratory aquariums, changes were detected in the protein metabolism components in the blood of fish. Total protein levels decreased by 29% in carp and 32% in crucian carp exposed to mycotoxin T-2. The study of ALT and AST activity in the blood under the influence of mycotoxin revealed an increase in the activity of aminotransferases in both carp and crucian carp. From the blood, free amino acids are transferred to the liver, where in response to ALT and AST, their transamination increases, explaining the heightened activity of these enzymes.

A peptide alleviated oxidative damages in the L02 cells and mice liver

The liver is vitally metabolic for a multitude of biochemical reactions. Consequently, it generates many free radicals and reactive oxygen species, rendering it more susceptible to oxidative stress-induced damage. Oxidative stress represents a pivotal factor in the pathogenesis of liver diseases. We screened some antioxidant peptides previously. Here we investigated whether the peptides could attenuate oxidative damage with APPH in L02 cells. The results showed that one of the peptides, sequence FETLMPLWGNK, could decrease the excessive reactive oxygen species, increase antioxidant enzyme activity and protect mitochondrial function, reduce the ratio of apoptosis and S phase cycle arrest, and improve the survival rate of L02 cells damaged by APPH compared to cells of the control group. Then the peptide was evaluated in mice that CCl4 injured. We found that CCl4-injured mice had significantly increased serum inflammatory factors and liver injury markers, a large number of inflammatory cell infiltration, and local necrosis in the liver. The peptide could reduce inflammation, and improve liver pathological changes. This phenomenon may be associated with the activation of the Nrf2 signaling pathway. Concurrently, the peptide protects the liver by regulating the expression of proteins related to the mitochondrial apoptosis pathway (p53, Bax, Bcl-2, and Caspase3) and mitophagy-related proteins (PINK1, Parkin, and AMPKα). Therefore, the results indicated that the peptide is an active substance with antioxidant activity and anti-inflammatory effects.

Biological effect of dual-frequency laser pulse radiation during pre-sowing treatment of rice seeds at early stages of development under stressful environmental conditions

The biological effect of two-frequency laser pulsed radiation produced on copper vapor during pre-sowing treatment of rice seeds of Veles and Leader varieties at early stages of development in extreme stressful environmental conditions has been studied. The copper vapor laser used in the experiment had the following output characteristics: wavelengths of 510.6 nm (green radiation line) and 578.2 nm (yellow radiation line), pulse duration of 15 ns, repetition frequency of 10 kHz, total pulse power of 10 kW, energy-power ratio between the green and yellow radiation lines 3/1 The interaction of laser radiation with wavelengths of 510.6 and 578.2 nm in the nonlinear medium of the seed caused the formation of additional wavelengths: a total wavelength of 271 nm (ultraviolet radiation) and a difference wavelength of 4.37 microns. Exposure to each of the four wavelengths in its spectral range could lead to the initiation of at least four radiation-induced biochemical reactions. It is shown that pre-sowing treatment of rice seeds with two-frequency laser pulsed radiation for 5–20 seconds had a stimulating effect on the growth and development of rice (the greatest effect was when exposed to 5–10 seconds), and also increased its stability when grown in extreme stressful environmental conditions (moisture deficiency, depleted soil) at early stages of development.

A machine learning framework for extracting information from biological pathway images in the literature

There have been significant advances in literature mining, allowing for the extraction of target information from the literature. However, biological literature often includes biological pathway images that are difficult to extract in an easily editable format. To address this challenge, this study aims to develop a machine learning framework called the “Extraction of Biological Pathway Information” (EBPI). The framework automates the search for relevant publications, extracts biological pathway information from images within the literature, including genes, enzymes, and metabolites, and generates the output in a tabular format. For this, this framework determines the direction of biochemical reactions, and detects and classifies texts within biological pathway images. Performance of EBPI was evaluated by comparing the extracted pathway information with manually curated pathway maps. EBPI will be useful for extracting biological pathway information from the literature in a high-throughput manner, and can be used for pathway studies, including metabolic engineering.

Role of biochar as a greener catalyst in biofuel production: Production, activation, and potential utilization – A review

BackgroundBiochar is an affordable carbonaceous product produced through a combination of thermochemical conversion processes using organic feedstock in the absence or constrained supply of oxygen. Biochar-based catalysts are promising in numerous catalytic applications for sustainable bioenergy production. MethodsHydrothermal carbonization, gasification, and pyrolysis are conventional biochar production techniques. The biochar must be activated to improve its physiochemical characteristics. The activation is generally classified into three types: physical, chemical, and impregnation. The biochar-based catalysts are categorized into different types, such as pristine, heteroatom-doped, metal-loaded, magnetic, and nanometallic oxide/hydroxide biochar. Significant findingsThese biochar-based catalysts effectively catalyze various biochemical reactions and are extensively used in biofuel production. This article extensively explores various biochar production and activation strategies and their applications as a catalyst for biofuel production. Activated biochar with improved surface properties has a high surface area and adaptable surface chemistry, making it a sustainable alternative to conventional catalysts. Biochar from renewable biomass has advantages in waste management, carbon sequestration, and reduced greenhouse gas emissions. Further research and innovation using nanomaterials, artificial intelligence, and machine learning are needed to utilize biochar as a sustainable and potential green catalyst for advanced biofuel production.

Unveiling the antiurolithiatic potentiality of two benzene sulfonamide derivatives against ethylene glycol-induced renal calculi

ObjectiveOxidative stress and inflammation play crucial roles in the onset of kidney injury and crystal formation caused by hyperoxaluria. Indapamide is a potent medication for treating renal calculi, but it has severe side effects such as hypokalemia, hypercalcemia, and hyperuricemia. Therefore, it is advisable to explore alternative treatments that do not have these side effects. The study aimed to reveal the antiurolithiatic potential of two benzene sulfonamide derivatives (SBCl and SBF; A and B, respectively) against ethylene glycol-induced kidney stones. MethodsThe rats were divided into two main groups: the first group consisted of 20 rats with induced kidney stones, and the second group included 15 control rats. This division enabled a comparative analysis between rats with kidney stones and those without, offering insights into the effects of kidney stone induction on various physiological parameters and biochemical markers. The effectiveness of benzene sulfonamide derivatives (compounds A and B) was assessed in rats with induced kidney stones. The treatment was given orally by gavage for 21 days, administered every 48h after inducing kidney stones with 0.12ml of 5% ethylene glycol (EG). ResultsThe influence of compounds A and B on electrolytes, biochemical, antioxidant, and inflammatory reactions in induced kidneys underscores their potential therapeutic advantages in alleviating the advancement of kidney stone disease and related complications. ConclusionBoth compounds were found to possess equal effectiveness in inhibiting the complications of stone formation. However, SBCl-EG showed superior antioxidant and inflammatory parameters effects compared to SBF-EG. Our study's findings underscore the potential benefits of derivatives in treating nephrolithiasis and related oxidative disorders, highlighting their superior effects on antioxidant and inflammatory responses compared to standard treatments.

Resilience of weighted networks with dynamical behavior against multi-node removal.

In many real-world networks, interactions between nodes are weighted to reflect their strength, such as predator-prey interactions in the ecological network and passenger numbers in airline networks. These weighted networks are prone to cascading effects caused by minor perturbations, which can lead to catastrophic outcomes. This vulnerability highlights the importance of studying weighted network resilience to prevent system collapses. However, due to many variables and weight parameters coupled together, predicting the behavior of such a system governed by a multi-dimensional rate equation is challenging. To address this, we propose a dimension reduction technique that simplifies a multi-dimensional system into a one-dimensional state space. We applied this methodology to explore the impact of weights on the resilience of four dynamics whose weights are assigned by three weight assignment methods. The four dynamical systems are the biochemical dynamical system (B), the epidemic dynamical system (E), the regulatory dynamical system (R), and the birth-death dynamical system (BD). The results show that regardless of the weight distribution, for B, the weights are negatively correlated with the activities of the network, while for E, R, and BD, there is a positive correlation between the weights and the activities of the network. Interestingly, for B, R, and BD, the change in the weights of the system has little impact on the resilience of the system. However, for the E system, the greater the weights the more resilient the system. This study not only simplifies the complexity inherent in weighted networks but also enhances our understanding of their resilience and response to perturbations.

Self-organization in computation and chemistry: Return to AlChemy.

How do complex adaptive systems, such as life, emerge from simple constituent parts? In the 1990s, Walter Fontana and Leo Buss proposed a novel modeling approach to this question, based on a formal model of computation known as the λ calculus. The model demonstrated how simple rules, embedded in a combinatorially large space of possibilities, could yield complex, dynamically stable organizations, reminiscent of biochemical reaction networks. Here, we revisit this classic model, called AlChemy, which has been understudied over the past 30 years. We reproduce the original results and study the robustness of those results using the greater computing resources available today. Our analysis reveals several unanticipated features of the system, demonstrating a surprising mix of dynamical robustness and fragility. Specifically, we find that complex, stable organizations emerge more frequently than previously expected, that these organizations are robust against collapse into trivial fixed points, but that these stable organizations cannot be easily combined into higher order entities. We also study the role played by the random generators used in the model, characterizing the initial distribution of objects produced by two random expression generators, and their consequences on the results. Finally, we provide a constructive proof that shows how an extension of the model, based on the typed λ calculus, could simulate transitions between arbitrary states in any possible chemical reaction network, thus indicating a concrete connection between AlChemy and chemical reaction networks. We conclude with a discussion of possible applications of AlChemy to self-organization in modern programming languages and quantitative approaches to the origin of life.

Optimizing Ligand-Receptor Binding Thermodynamics and Kinetics: The Role of Terahertz Wave Modulation in Molecular Recognition

Precision control over ligand-receptor recognition is critical in biochemistry and pharmacology. Traditional methods that alter reaction environments have limitations in fine-tuning the thermodynamic and kinetic aspects of biochemical reactions within biological systems. The advent of terahertz wave technology represents a significant breakthrough, providing a refined approach to modulating ligand-receptor interactions. This perspective explores the cutting-edge potential of terahertz waves in refining ligand-receptor recognition, featuring their innovative application in modulating neuronal functions. The capabilities of terahertz technology to selectively influence molecular interactions are discussed, highlighting its transformative potential for advancing therapeutic strategies and deepening our understanding of biological mechanisms.

Decomposition of Hydrogen Peroxide in Presence of DimethylglyoximatoNickel Complexes as Catalysts: Catalase-Like Activity

The development in coordination chemistry in recent years raise hopes that synthetically produced metal complexes could mimic many biochemical systems widely found in nature. There is a certain analogy between nature and organometallic systems. A large number of biological metal complexes are known, including oxygen carriers like hemoglobin in the blood, which contains a ferrous ion; respiratory enzymes; those involved in protein hydrolysis; and vitamin B12, which is only active in the presence of cobalt in the trivalent state. The Nickel (III), in addition to Fe-S clusters, was an essential component in hydrogenases. Since then, nickel (III) complexes have been used as models for studying the catalytic function of certain enzymes (hydrogenases). In this context, a study on the catalytic ability of dimethylglyoximato-nickel complexes as peroxiredoxases in the dismutation or oxidation of hydrogen peroxide was conducted. The results were discussed, commented upon, and a reaction mechanism was proposed. The results seem encouraging, regarding the effect of the complexation on catalase- like activity.

The Physical Sensation of Precordial Pain as a Signal of Psychiatric Relevance of Anguish

Anguish is a sensation of precordial chest tightness or oppression that has an emotional origin. Unlike anxiety which is focused on the future, anguish is experienced in the present as a state of pain and mental agony. Furthermore, distress is supposed to have different autonomic, physiological and biochemical reactions when compared to anxiety and depression. The main objective of this work is to investigate whether depression is more related to anguish than anxiety, and also to identify which variables or symptoms are most predictive of the state of anguish. A binomial logistic regression model was adjusted and statistically significant evidence was found that depression may be more related to distress than anxiety. An important depression diagnostic variable (MINI Depression) was selected in the model (at the 10% level) and its interpretation corroborates the central hypothesis of the study mentioned above. Correspondence analysis also points to clues in the direction of the research hypothesis. As for the second objective, under the same logistic model, the following variables were shown to be related to the state of distress: Gender, Reduced Hamilton Score, BSI Somatization, BSI Hostility, BSI Obsession Compulsion, Age and MINI Depression.

Isolation and Identification of Highly Sb-Resistant Rhodotorula glutinis Strain J5 and its Mechanism of Resistance to Sb(III).

Sb-resistant strains can detoxify antimony through metabolic mechanisms such as oxidation and affect the migration, transformation, and ultimate fate of antimony in the environment. In this study, a strain of Sb-resistant fungi, Rhodotorula glutinis sp. Strain J5, was isolated from Xikuangshan mine and its growth characteristics, gene expression differences, and functional annotation under Sb(III) stress were further investigated to reveal the mechanism of resistance to Sb(III). We identified strain J5 as belonging to the Rhodotorula glutinis species optimally growing at pH 5.0 and at 28°C of temperature. According to gene annotation and differential expression, the resistance mechanism of Strain J5 includes: reducing the endocytosis of antimony by aquaporin AQP8 and transmembrane transporter pst, enhancing the efflux of Sb(III) by the gene expression of acr2, acr3 and ABC, improving the oxidation of Sb(III) by iron-sulfur protein and Superoxide dismutase (SOD), glutathione (GSH) and cysteine (Cys) chelation, methylation of methyltransferase and N-methyltransferase, accelerating cell damage repair and EPS synthesis and other biochemical reaction mechanisms. FT-IR analysis shows that the -OH, -COOH, -NH, -PO, C-O, and other active groups of Strain J5 can be complexed with Sb(III), resulting in chemical adsorption. Strain J5 displays significant resistance to Sb(III) with the MIC of 1300mg/L, playing a crucial role in the global biochemical transformation of antimony and its potential application in soil microbial remediation.