Objective: To determine the frequency of Hypovitaminosis D in kids by increasing pains and to find out the relationships among biochemical biomarkers and serum vitamin D concentration levels. Study Design: An exploratory cross-sectional investigation was conducted. Methodology: Growing pains were diagnosed in 100 outpatient children aged 5–12 with diffuse lower leg pains. The study excluded infants with arthritis, systemic diseases, organic pain, rheumatologic diseases, or rickets symptoms. We measured total inorganic phosphate, calcium, alkaline phosphatase, and vitamin D3 (25-hydroxycholecalciferol). Group A had enough vitamin D3 (> 75 nmol/L), Group B had 50–75, and Group C had levels below 50. Group rates mattered at 0.05 in the chi-square test. Results: 9-year-olds and 60% women participated. 7% of infants with growth pains had enough vitamin D. 95% of vitamin D-deficient babies had high alkaline phosphatase. Conclusion: Growth pains may cause hypovitaminosis D. Youngster's Vitamin D levels should be tested for unusual leg pain. Molecular clues cannot diagnose hypovitaminosis D.