Screening potential toxicity of currently used herbicides in the freshwater amphipod Gammarus fossarum based on multi-level biomarker responses to field-realistic exposures

Abstract

Herbicides are widely used to control weeds and maximize crop growth. Because of agricultural runoff, these chemicals are potentially hazardous to aquatic wildlife. However, their ecotoxicity and resulting disturbance in individual performance remain scarcely documented in freshwater crustaceans. This study aimed to screen the potential toxicity of currently used herbicides in the ecosystem engineer Gammarus fossarum using multi-level biomarkers. In microcosms, gammarids were exposed for 72 h to 12 herbicides individually (quinmerac, mesotrione, bentazone, isoproturon, chlortoluron, metazachlor, chloridazone, diflufenican, flufenacet, aclonifen, prosulfocarb and metolachlor) at a field-realistic concentration (i.e. 10 μg/L). The sublethal effects were assessed by monitoring several biochemical, physiological and behavioural traits. In exposed gammarids, alterations in behavioural activities were observed, i.e. increased locomotion and respiration as a general trend. Moreover, biochemical biomarkers suggested herbicide-dependent disruptions in moulting, antioxidant responses and cell integrity. Integrating multi-metric variations through statistical analyses allowed us to identify herbicide clusters likely to trigger common sets of biological responses. Depressed antioxidant defence at the cell level and impaired respiration at the individual level were the predominant toxic effects of herbicides, related to their hydrophobic feature. Furthermore, establishing relationships between sublethal alterations in gammarids and acute lethality or chronic toxicity values defined for regulatory purposes supports the relevance of these alterations as early warnings of toxicity. Our findings demonstrate that currently used herbicides have unexpected toxicological effects in a non-target wild animal, with possible long-term alterations in population dynamics and associated ecological functions, which constitute promising diagnostic tools for risk assessment in agricultural areas.