Bioavailability Of Cd In Soil Research Articles

Assessment of Cd bioavailability using chemical extraction methods, DGT, and biological indicators in soils with different aging times

Total cadmium (Cd) cannot be used to accurately assess the ecological risk of Cd pollution in soil. Currently there is no universally recognized method to evaluate Cd bioavailability in soil. In this study, chemical extraction methods, diffusive gradients in thin films (DGT) and bioindicator methods were used to evaluate Cd bioavailability in soils with the same properties but different aging times. Results indicate that aging decreased the Cd bioavailability in soil and its toxicity to barley. This was primarily due to a decrease in the proportion of ion-exchangeable Cd. Correlation analyses were conducted on the Cd bioavailable content obtained via the soil extraction methods and the toxicity effect of barley. Results showed that the order of the minimum value of the linear regression determination coefficient (R2) of chemical extraction methods and DGT was as follows: DGT-Cd (0.7385, p < 0.05) > total Cd (0.6931, p < 0.05) > acetic acid-Cd (0.6078) > ion-exchangeable Cd (0.5933) > DTPA-Cd (0.5842) > CaCl2-Cd (0.4980) > water-soluble Cd (0.4602). The order of minimum value of R2 of biological indicators of barley was integrated biomarker response (IBR) (0.8501, p < 0.01) > length (0.6492) > dry weight (0.6320) > fresh weight (0.4980) > Cd concentration (0.4602). The root is more suitable for indicating the plant uptake and accumulation of Cd in soil. Meanwhile, the shoot can effectively evaluate the toxic effect of Cd stress on plants. DGT is more suitable to reflect Cd bioavailability to barley compared to chemical extraction methods, Furthermore, it can be used to evaluate stable polluted soil with longer aging time. In the study of the bioavailability of heavy metals in soil, IBR can be used as a reliable reference index to contribute to the comprehensive evaluation of metal bioavailability in addition to considering plant uptake.

Research Advances in Cadmium Uptake, Transport and Resistance in Rice (Oryza sativa L.).

Rice (Oryza sativa L.) is one of the most important food crops, feeding half of the world’s population. However, rice production is affected by cadmium (Cd) toxicity. Due to an increase in Cd-contaminated soil and rice grains, and the serious harm to human health from Cd, research on Cd uptake, transport and resistance in rice has been widely conducted, and many important advances have been made. Rice plants absorb Cd mainly from soil through roots, which is mediated by Cd absorption-related transporters, including OsNramp5, OsNramp1, OsCd1, OsZIP3, OsHIR1,OsIRT1 and OsIRT2. Cd uptake is affected by soil’s environmental factors, such as the concentrations of Cd and some other ions in soil, soil properties, and other factors can affect the bioavailability of Cd in soil. Then, Cd is transported within rice plants mediated by OsZIP6, OsZIP7, OsLCD, OsHMA2, CAL1, OsCCX2, OsLCT1 and OsMTP1, from roots to shoots and from shoots to grains. To resist Cd toxicity, rice has evolved many resistance strategies, including the deposition of Cd in cell walls, vacuolar Cd sequestration, Cd chelation, antioxidation and Cd efflux. In addition, some unresolved scientific questions surrounding Cd uptake, transport and resistance in rice are proposed for further study.

Regrow Napier grass–Chinese milk vetch relay intercropping system: A cleaner production strategy in Cd-contaminated farmland

The cultivation methods determine the production and remediation capacity of crops in heavy metal-contaminated farmland. This study investigated the effects of seedling establishment methods and relay intercropping with Chinese milk vetch (Astragalus sinicus L., CMV) on the production and remediation of Napier grass (Pennisetum purpureum Schum, NG) in cadmium (Cd)-contaminated farmland in southern China. The results showed that the regrow NG (RNG) had higher aerial part yield because of its well-developed roots and larger tiller numbers, which increased the Cd uptake. CMV mulching promoted the stubble seedling's growth by increasing the soil temperature during the overwintering period. The CMV return to field increased the soil dissolved organic carbon (DOC), enhanced the bioavailability of Cd in soil, and promoted the Cd uptake of NG. The CMV application also alleviated the total nitrogen decline of soil. The CMV–RNG relay intercropping system (CMV–RNG) produced larger aerial part yield and Cd uptake, mowing NG once (in December), reaching 60.60 t ha−1 (DW) and 29.09 g ha−1, respectively, and August was the suitable month to mow for higher yield and Cd uptake. Meanwhile, the Cd concentration in NG aerial parts was relatively low (0.16–0.59 mg kg−1), making it safe to be used as fodder. Thus, the CMV–RNG system is a cleaner production strategy in Cd-contaminated farmland.

Application of cotton straw biochar and compound Bacillus biofertilizer decrease the bioavailability of soil cd through impacting soil bacteria

BackgroundCd seriously threatens soil environment, remedying Cd in farmland and clearing the response of soil environment to modifiers in Cd-contaminated soils is necessary. In this study, the effects of cotton straw biochar and compound Bacillus biofertilizer used as modifiers on the biochemical properties, enzyme activity, and microbial diversity in Cd-contaminated soils (1, 2, and 4 mg·kg−1) were investigated.ResultsThe results showed that both cotton straw biochar and compound Bacillus biofertilizer could improve the soil chemical characteristics, including the increase of soil C/N ratio, electrical conductance (EC) and pH, and the most important decrease of soil available Cd content by 60.24% and 74.34%, respectively (P < 0.05). On the other hand, adding cotton straw biochar and compound Bacillus biofertilizer in Cd stressed soil also improved soil biological characteristics. Among them, cotton straw biochar mainly through increasing soil alkaline phosphatase activity and improve bacteria abundance, compound Bacillus biofertilizer by increasing soil invertase, alkaline phosphatase, catalase, and urease activity increased bacterial community diversity. On the whole, the decrease of soil available Cd was mainly caused by the increase of soil pH, C/N, urease and alkaline phosphatase activities, and the relative abundance of Acidobacteria and Proteobacteria.ConclusionsIn summary, the applications of cotton straw biochar and compound Bacillus biofertilizer could decrease soil available Cd concentration, increase soil bacterial community diversity and functions metabolism, and reduce the damage of Cd stress, compared with cotton straw biochar, compound Bacillus biofertilizer was more effective in immobilizing Cd and improving soil environmental quality.

Effects of exogenous additives on wheat Cd accumulation, soil Cd availability and physicochemical properties in Cd-contaminated agricultural soils: A meta-analysis

Cadmium (Cd) contamination in wheat is a serious issue. The application of exogenous additives can effectively inhibit Cd bioavailability in soil and decrease Cd accumulation in wheat. However, a comprehensive and quantitative analysis of how additives affect wheat Cd accumulation, wheat yield, soil Cd availability, and soil properties is lacking. We conducted a meta-analysis of 65 peer-reviewed papers published before April 2021 to investigate how additives application affects Cd accumulation in wheat and soil Cd availability.The results indicated that most additives application decreased the diethylenetriaminepentaacetic acid extractable-Cd content (5.27–56.33%) in the soil, and wheat grain and root Cd concentrations (0.03–129.87% and 0.42–52.84%, respectively); the pH values of wheat-grown soil and the properties of the additives affected the reduction percentage. Overall, most wheat-grown soils were calcareous soil (42 peer-reviewed papers); in calcareous soil, the magnitude of the Cd reduction in wheat grain was the highest under treatments with clay minerals (129.87%) due to clay modification, followed by composite (75.36%) and phosphorus materials (73.55%). Moreover, most additives application increased wheat grain yield by 0.03–51.84%, which was attributed to the positive effects of additives on wheat antioxidant capacity, photosynthesis, respiration, and nutrient uptake. Additives application increased the pH value of acidic wheat soil, and positively affected the electrical conductivity, cation exchange capacity, and organic carbon content of the wheat grown soil. In addition, regression analysis showed that soil available Cd was negatively correlated with the pH value with additives application in acidic soil (r2 = 0.43), while a non-significant correlation was observed in neutral and calcareous wheat soils (r2 = 0.017 and 0.016, respectively). The results of this study can assist in the selection, modification, and utilisation of additives to remediate Cd-contaminated wheat soils.

Leveraging high-throughput hyperspectral imaging technology to detect cadmium stress in two leafy green crops and accelerate soil remediation efforts

Cadmium (Cd) is a toxic metal that can accumulate in soils and negatively impact crop as well as human health. Amendments like biochar have potential to address these challenges by reducing Cd bioavailability in soil, though reliance on post-harvest wet chemical methods to quantify Cd uptake have slowed efforts to identify the most effective amendments. Hyperspectral imaging (HSI) is a novel technology that could overcome this limitation by quantifying symptoms of Cd stress while plants are still growing. The goals of this study were to: 1) determine whether HSI can detect Cd stress in two distinct leafy green crops, 2) quantify whether a locally sourced biochar derived from hardwoods can reduce Cd stress and uptake in these crops, and 3) identify vegetative indices (VIs) that best quantify changes in plant stress responses. Experiments were conducted in a tightly controlled automated phenotyping facility that allowed all environmental factors to be kept constant except Cd concentration (0, 5 10 and 15 mg kg−1). Symptoms of Cd stress were stronger in basil (Ocimum basilicum) than kale (Brassica oleracea), and were easier to detect using HSI. Several VIs detected Cd stress in basil, but only the anthocyanin reflectance index (ARI) detected all levels of Cd stress in both crop species. The biochar amendment did reduce Cd uptake, especially at low Cd concentrations in kale which took up more Cd than basil. Again, the ARI index was the most effective in quantifying changes in plant stress mediated by the biochar. These results indicate that the biochar evaluated in this study has potential to reduce Cd bioavailability in soil, and HSI could be further developed to identify rates that can best achieve this benefit. The technology also may be helping in elucidating mechanisms mediating how biochar can influence plant growth and stress responses.

The Combined Effect of Pseudomonas stutzeri and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (Lactuca sativa) to Cadmium Stress

Agricultural activities lead to the accumulation of cadmium (Cd) in the soil. It is necessary to identify effective and economical ways to reduce the soil Cd bioavailability. To achieve this, three bacterial strains, Pseudomonas stutzeri, P. koreensis, and P. fluorescens, were tested for tolerance and biosorption of different concentrations of Cd (0, 5, 10, 15, 20, and 25 mg L−1). During the 2020 and 2021 seasons, a pot experiment was conducted using four different soil amendments (control, biochar, P. stutzeri, and a combination) under four levels of Cd (0, 40, 80, and 120 mg kg−1) and assessing the effect on growth parameters, physiological modifications, antioxidant enzymes, and Cd accumulation in lettuce plants (Lactuca sativa cv. Balady). In vitro, the results showed that P. stutzeri was the most tolerant of Cd. Our findings in pot trials showed that T4 (biochar + P. stutzeri) was a more efficient treatment in terms of the growth parameters, with 452.00 g plant−1 was recorded for fresh weight, 40.10 g plant−1 for dry weight, 18.89 cm plant−1 for plant height, 6.03 cm2 for leaf area, and 20.48 for the number of leaves plant−1, while in terms of physiological characteristics, we recorded 1.29 mg g−1 FW, 0.35 μg g−1 FW, and 3.69 μg g−1 FW for total chlorophyll, carotenoids, and total soluble sugar, respectively; this was also reflected in the number of antioxidant enzymes and intensity of soil biological activities in soil treated with 120 mg kg−1 Cd compared with the control and other treatments in the first season. A similar trend was observed in the second season. Additionally, significantly lower Cd was observed in both the root (67%) and shoots (78%). Therefore, a combined application of biochar and P. stutzeri could be used as an alternative to mitigate Cd toxicity.

Organic Acid Excretion in Root Exudates as a Mechanism of Cadmium Uptake in a Sonchus Asper-Zea Mays Intercropping System.

This study assessed how the Sonchus asper L.-Zea mays intercropping influenced the plant Cd level, as well as the organic acid (low molecular mass) types and concentrations in root secretions, which was accomplished by conducting pot experiments with soil that was collected from a farmland surrounding a mining area. The results showed that the oxalic and citric acids were the prevailing organic acids in the plant root secretions, and that the intercropping led to prominently elevated root exudate concentrations of the two acids for S. asper by 43.8 and 75.4%, respectively, while decreased such concentrations by 18.5 and 18.7% for Z. mays, compared to monoculture. According to observations, the root exudate concentrations of citric and oxalic acids were significantly positively related to the available rhizosphere soil Cd and plant Cd. The results indicate that changes in root exudation of oxalic acid and citric acid result in different available Cd contents of the rhizosphere soil, thereby affecting the bioavailability of soil Cd, which increases Cd uptake and accumulation in S. asper but inhibits Cd accumulation in Z. mays.

Microbial induced carbonate precipitation contributes to the fates of Cd and Se in Cd-contaminated seleniferous soils

Bioremediation based on microbial induced carbonate precipitation (MICP) was conducted in Cd-contaminated seleniferous soils with objective to investigate effects of MICP on the fates of Cd and Se in soils. Results showed that soil indigenous microorganisms could induce MICP process to stabilize Cd and mobilize Se without inputting exogenous urease-producing strain. After remediation, soluble Cd (SOL-Cd) and exchangeable Cd (EXC-Cd) concentrations were decreased respectively by 59.8% and 9.4%, the labile Cd measured by the diffusive gradients in thin-films technique (DGT) was decreased by 14.2%. The MICP stabilized Cd mainly by increasing soil pH and co-precipitating Cd during the formation of calcium carbonate. Compared with chemical extraction method, DGT technique performs better in reflecting Cd bioavailability in soils remediated with MICP since this technique could eliminate the interference of Ca2+. The increase in pH resulted in Se conversion from nonlabile fraction to soluble and exchangeable fractions, thus improving Se bioavailability. And Se in soil solution could adsorb to or co-precipitate with the insoluble calcium carbonate during MICP, which would partly weaken Se bioavailability. Taken together, MICP had positive effects on the migration of Se. In conclusion, MICP could stabilize Cd and improve Se availability simultaneously in Cd-contaminated seleniferous soils.

Influence of light-irradiated Noccaea caerulescens on the characteristics of dissolved organic matter in its rhizospheric soil during phytoremediation.

It has been observed that suitable light irradiation can improve the phytoremediation efficiency of various plants by enhancing their growth rate and metal uptake capacity. However, the mechanisms underlying the effects of light irradiation on metal mobilization and translocation in soils have rarely been reported. This experiment was conducted to evaluate the variation in dissolved organic matter (DOM) in the rhizosphere of Noccaea caerulescens (formerly Thlaspi caerulescens J. & C. Presl) when irradiated with different combinations of red (0, 25, 50, 90, and 100% red) and blue light. N. caerulescens was induced to secrete significantly more DOM, relative to the control, into its rhizosphere after being irradiated with pure red light and other red light combinations; this increased the bioavailability of soil Cd. Moreover, the concentrations and proportions of the hydrophilic DOM fractions, particularly hydrophilic acid, which exhibited a high affinity for Cd, increased with increasing ratios of the red light. Furthermore, DOM secreted because of the light irradiation treatments exhibited a significantly higher Cd extraction ability compared with that of the untreated control; this consequently increased the Cd uptake capacity of N. caerulescens. The results demonstrated that the secretion of more DOM, particularly hydrophilic acid, plays a pivotal role in improving the phytoremediation efficiency of N. caerulescens.

In situ cadmium removal from paddy soils by a reusable remediation device and its health risk assessment in rice

Passivation is widely used in the remediation of cadmium (Cd) polluted soils. However, this technique could only reduce the bioavailability of soil Cd rather than remove Cd from the soils. Herein, it is crucial to develop an efficient in-situ technique to remove soil Cd, and further to uncover its effect and mechanism on crops and soil environment. In this study, a soil pot experiment was employed to investigate the effects of a well-designed reusable device on in situ Cd removal from the two paddy soils (red soil and brown soil), rice (Oryza sativa L) growth, and the associated health risk of Cd. The device contained three typical soil Cd amendments including cation exchange resin (CER), biochar (BC), and steel slag (SS). Each of the soil amendments was combined with slow-release fertilizer (SRF) or conventional compound fertilizers (CF). The results indicated that the device with CER, BC, and SS decreased soil Cd by 12.6%, 16.2%, and 19.8%, respectively. Thus, it reduced the rice Cd accumulation by 30.6%, 16.1%, and 29%, and increased the rice yield by 70.3%, 42.6%, and 55.4%, respectively. This could be stemmed from the improvement of photosynthesis and the reduction of oxidative damage, as well as the increase in the relative abundance of soil beneficial microbial community. Moreover, the grey correlation degrees and human health risk index (HRI) indicated that the in-situ removal device could diminish the health risk of Cd in rice. The findings reported in this work could provide a new solution to remediate heavy metal contaminated soils.

Reducing cadmium in rice using metallothionein surface-engineered bacteria WH16-1-MT

Cadmium (Cd) accumulation in rice grains poses a health risk for humans. In this study, a bacterium, Alishewanella sp. WH16-1-MT, was engineered to express metallothionein on the cell surface. Compared with the parental WH16-1 strain, Cd2+ adsorption efficiency of WH16-1-MT in medium was increased from 1.2 to 2.6 mg/kg dry weight. The WH16-1-MT strain was then incubated with rice in moderately Cd-contaminated paddy soil. Compared with WH16-1, inoculation with WH16-1-MT increased plant height, panicle length and thousand-kernel weight, and decreased the levels of ascorbic acid and glutathione and the activity of peroxidase. Compared with WH16-1, WH16-1-MT inoculation significantly reduced the concentrations of Cd in brown rice, husks, roots and shoots by 44.0 %, 45.5 %, 36.1 % and 47.2 %, respectively. Moreover, inoculation with WH16-1-MT reduced the bioavailability of Cd in soil, with the total Cd proportion in oxidizable and residual states increased from 29 % to 32 %. Microbiome analysis demonstrated that the addition of WH16-1-MT did not significantly alter the original bacterial abundance and community structure in soil. These results indicate that WH16-1-MT can be used as a novel microbial treatment approach to reduce Cd in rice grown in moderately Cd-contaminated paddy soil.

Novel agricultural waste-based materials decrease the uptake and accumulation of cadmium by rice (Oryza sativa L.) in contaminated paddy soils

Heavy metal pollution in paddy fields has caused widespread concerns due to the threat to food safety. The present study used low-cost sugarcane bagasse (SB) and two sugarcane bagasse materials modified with citric-acid (SSB) and citric-acid/Fe3O4 (MSB) to investigate their effects on the bioavailability of Cd in soil and Cd accumulations in rice in a pot experiment. The three organic amendments significantly decreased the Cd accumulation in plants by limiting its mobilization in soil. The MSB and SSB but not SB increased the soil pH and immobilized the Cd in soil significantly during the 120-day experiment. The amendments decreased Cd bioavailability through transforming to the stable fraction throughout the whole growth stage. The functional groups in the amendments (-OH, –COOH, C–O, -COO- and Fe–O) and precipitates [Cd(NO2)2K(NO2)2, Cd(OH)2 and Cd75Zn25Fe2O4] played active roles in Cd immobilization. Moreover, the three organic materials increased the content of Fe–Mn plaque on rice roots, which prevented its transport from soil to rice roots further. We also found that Fe competed with Cd for transporters and reduced potential Cd uptake and translocation in rice tissues. The addition of MSB and SB but not SSB inhibited the rice growth compared to the unamended control, indicating the potential of SSB in situ remediation. These results provide valuable information to use organic amendments for Cd passivation in soil and food safety.

Supplying silicon alters microbial community and reduces soil cadmium bioavailability to promote health wheat growth and yield

Soil amendments of black bone (BB), biochar (BC), silicon fertilizer (SI), and leaf fertilizer (LF) play vital roles in decreasing cadmium (Cd) availability, thereby supporting healthy plant growth and food security in agroecosystems. However, the effect of their additions on soil microbial community and the resulting soil Cd bioavailability, plant Cd uptake and health growth are still unknown. Therefore, in this study, BB, BC, SI, and LF were selected to evaluate Cd amelioration in wheat grown in Cd-contaminated soils. The results showed that relative to the control, all amendments significantly decreased both soil Cd bioavailability and its uptake in plant tissues, promoting healthy wheat growth and yield. This induced-decrease effect in seeds was the most obvious, wherein the effect was the highest in SI (52.54%), followed by LF (43.31%), and lowest in BC (35.24%) and BB (31.98%). Moreover, the induced decrease in soil Cd bioavailability was the highest in SI (29.56%), followed by BC (28.85%), lowest in LF (17.55%), and BB (15.30%). The significant effect in SI likely resulted from a significant increase in both the soil bioavailable Si and microbial community (Acidobacteria and Thaumarchaeota), which significantly decreased soil Cd bioavailability towards plant roots. In particular, a co-occurrence network analysis indicated that soil microbes played a substantial role in wheat yield under Si amendment. Therefore, supplying Si alters the soil microbial community, positively and significantly interacting with soil bioavailable Si and decreasing Cd bioavailability in soils, thereby sustaining healthy crop development and food quality.

Biomineralization of Cd2+ and inhibition on rhizobacterial Cd mobilization function by Bacillus Cereus to improve safety of maize grains

Reducing cadmium (Cd) bioavailability and rhizobacterial Cd mobilization functions in the rhizosphere via the inoculation of screened microbial inoculum is an environmental-friendly strategy to improve safety of crop grains. In this study, Bacillus Cereus, a model Cd resistant strain, was selected to explore its effects on Cd bioavailability and uptake, bacterial metabolic functions related to Cd mobilization. Results indicated that inoculation of Bacillus Cereus in maize roots of sand pot with water-soluble Cd (0.06–0.15 mg/kg) and soil pot with high Cd-contaminated soil (total Cd: 2.33 mg/kg; Cd extracted by NH4NO3: 38.6 μg/kg) could decrease water-soluble Cd ion concentration by 7.7–30.1% and Cd extracted with NH4NO3 solution by 7.8–22.5%, inducing Cd concentrations in maize grains reduced by 10.6–39.9% and 17.4–38.6%, respectively. Even for a single inoculation in soil, Cd concentration in maize grains still satisfy food safety requirements (Cd content: 0.1 mg/kg dry weight) due to its successful colonization on root surface of maize. Bacillus Cereus could enrich more plant growth promotion bacteria (PGPB) and down-regulate the expression of genes related to bacterial motility, membrane transports, carbon and nitrogen metabolism in the rhizosphere soil, decreasing Cd bioavailability in soil. Approximately 80% Cd2+ in media was transferred into intracellular, meanwhile Cd salts (sulfide and/or phosphate) were produced in Bacillus Cereus through biomineralization process. Overall, this study could provide a feasible method for improving safety of maize grains via the inoculation of Bacillus Cereus under Cd pollution.

Combining multisurface model and Gouy–Chapman–Stern model to predict cadmium uptake by cabbage (Brassica Chinensis L.) in soils

Cadmium is an extremely toxic substance known to cause serious health problems. The uptake of Cd in plants is critically affected by dissolved Cd in soil porewater, controlled by soil physicochemical properties. Rhizo-availability of Cd is assumed, as the Cd fraction is found on the plasma membrane of surface root cells. Based on the theory of Cd transformation in soil–crop systems, we established a novel combined mechanistic model related to soil, soil solutions, and crops. The combined model comprises a multisurface model (MSMs; solid adsorbent and porewater) and the Gouy–Chapman–Stern model (GCS; porewater and root surface). The results suggested that in mildly contaminated soil samples, optimum prediction was achieved when DTPA-extractable Cd was used as input variable (R2 = 0.723). Our approach was superior to single-step model calculation (MSMs: R2 = 0.613; GCS: R2 = 0.629) and prediction based on extractable soil Cd (R2 = 0.281). Introducing DTPA extraction expanded the range of model applications at different soil pHs. Our proposed mechanism model was based on soil physicochemical properties for Cd migration from soil to cabbage. Our model showed promise in predicting Cd bioavailability in soil with a wide pH range and evaluating soil risk near the standard Cd safety level.

Impact of Biochar on Soil Properties, Pore Water Properties, and Available Cadmium

Some effects of biochar on soil properties (such as pore water DOC) are not very clear. The changes of soil properties [cation exchange capacity (CEC)], pore water properties [pH, dissolved organic carbon (DOC), and Cd concentration (CPW-Cd)], Cd concentration measured by diffusive gradients in thin films (CDGT-Cd), and available Cd content (Cd in weak acid extractable state and reducible state, CBCR-Cd) determined by the BCR sequential extraction procedure over time after biochar addition were studied by soil incubation and potted corn experiments with five soils from a mining area. The results showed increases of 20.3%-64.6% in CEC and 0.34-1.02 in pH (both p < 0.05) in the soil incubation after adding biochar. The DOC concentration was reduced by 8.2%-33.2% (p < 0.05). CPW-Cd, CDGT-Cd, and CBCR-Cd decreased by 14.2%-47.2%, 15.3%-47.9%, and 22.3%-61.4%, respectively. During the corn cultivation phase, CEC increased by 5.1%-29.0%, and DOC concentration decreased by 10.4%-41.3% (p < 0.05). CPW-Cd, CDGT-Cd, and CBCR-Cd decreased by 5.9%-22.4%, 7.2%-25.1%, and 10.5%-64.8%, respectively. Biochar effectively increased the biomass of corn roots and reduced the concentration of Cd in the roots. Biochar altered the properties of soil and pore water, reduced the bioavailability of Cd in soil, and mitigated the harm to corn caused by Cd.

Cd immobilization and soil quality under Fe–modified biochar in weakly alkaline soil

Cost–effective and environment–friendly implementation techniques are critical to the success of remediation in large–scale cadmium (Cd) contaminated agricultural soil. Field experiments were conducted to investigate the effect of Fe–modified biochar on Cd bioavailability in soils and uptake by maize (Zea mays L.), soil aggregate distribution and stability, and microbial community composition in weakly alkaline Cd–contaminated soil. Results showed that Fe–modified biochar optimized the structure and stability of soil aggregates. Moreover, the content of soil organic carbon increased by 6.59%–20.36% when compared with the control groups. However, DTPA–Cd concentration under the treatment of Fe–modified biochar was suffered by 37.74%–41.65% reduction in contrast with CK, and the significant decrease (P < 0.05) was obtained at 0.5% Fe–modified biochar. Moreover, sequential extraction procedures showed that the acid soluble and reducible states of Cd was converted into oxidizable and residual form. The addition of Fe–modified biochar inhibited Cd accumulation in maize, being 41.31%–76.64% (Zhengdan 958), 38.19%–70.95% (Liyu 86) and 52.30%–59.95% (Sanbei 218) reduction, respectively, in contrast with CK. The activity of catalase, urease and alkaline phosphatase in soil increased gradually with the addition of Fe–modified biochar. The enhancement in the number of soil bacterial OTUs and the values of Shannon, Chao1, ACE index indicated that Fe–modified biochar promoted the richness and diversity of bacterial communities. Therefore, the improvements of soil environment and biological quality indicated that Fe–modified biochar should be an alternative agent on remediation of Cd–contaminated soils.

Polyethylene microplastics increase cadmium uptake in lettuce (Lactuca sativa L.) by altering the soil microenvironment

Little research has focused on the combined pollution of microplastics (MPs) and heavy metals in soil, especially the mechanism of their interaction. We conducted a 45-day microcosm experiment to test the hypothesis that polyethylene (PE) MPs and cadmium (Cd) had a joint toxicity to lettuce fitness. The effects of MPs at different addition ratios on Cd bioavailability and soil properties were also investigated in the microenvironment of three levels of Cd-contaminated soils. The results showed that the 10% MPs had an adverse impact on the plant biomass and significantly decreased soil pH and cation exchange capacity (CEC), but significantly increased soil dissolved organic carbon (DOC). The presence of MPs increased the soil Cd bioavailability and plant Cd concentrations and accumulations across all three levels of Cd-contaminated soils, which potentially aggregated the combined toxicity. The amounts of the bacterial 16SRNA and the fungal ITSRNA genes displayed a hormesis effect in response to the MP addition ratios while the abundance of Cd resistance genes cadA and czcA increased across all three Cd levels. The regression path analysis indicated that MPs affected shoot Cd concentrations by altering soil properties, which directly and indirectly contributed to the alteration mechanism, while the soil pH, DOC, and Cd bioavailability played core roles. The results suggest that the co-exposure of PE MPs in heavy metal-contaminated soil may therefore increase the toxicity, uptake, accumulation, and bioavailability of heavy metals by altering the properties of the soil microenvironment, which deserves further research.

Short-Term Aging of Pod-Derived Biochar Reduces Soil Cadmium Mobility and Ameliorates Cadmium Toxicity to Soil Enzymes and Tomato.

Contamination of agricultural soil with cadmium (Cd) has become a global concern because of its adverse effects on ecohealth and food safety. Soil amendment with biochar has become one of the phytotechnologies to reduce soil metal phyto-availability and its potential risks along the food chain. Biochar, derived from cocoa pod, was evaluated in soil Cd fractions (exchangeable, reducible, oxidizable, and residual) by modified Commission of the European Communities Bureau of Reference sequential extraction and its efficacy to ameliorate Cd toxicity to soil enzymes and leaf bioactive compounds. A pot experiment was conducted using Cd-spiked soil at 10 mg/kg with tomato (Solanum lycopersicum L.) at a biochar application rate of 1 and 3% (w/w) for 6 wk. The addition of biochar significantly reduced (p < 0.05) the exchangeable, reducible, and residual fractions by at least approximately 23%, with a consequential decrease in Cd root uptake and transport within tomato tissues. The activity of soil enzymes (catalase, dehydrogenase, alkaline phosphatase, and urease) was affected by Cd toxicity. However, with the exception of dehydrogenase, biochar application significantly enhanced the activity of these enzymes, especially at the 3% (w/w) rate. As for the secondary metabolites we studied, Cd toxicity was observed for glutathione, terpenoids, and total phenols. However, the biochar application rate of 1% (w/w) significantly ameliorated the effects of toxicity on the secondary metabolites. In conclusion, biochar demonstrated the potential to act as a soil amendment for Cd immobilization and thereby reduce the bioavailability of Cd in soil, mitigating food security risks. Environ Toxicol Chem 2021;40:3306-3316. © 2020 SETAC.