The main challenges with using biodiesel in CI engines are higher NOx emissions and reduced brake thermal efficiency. These biodiesel-related issues can be handled by concurrently introducing gaseous fuels along with primary fuel into CI engines. This study examines the effects of hydrogen in a dual fuel mode operation with diesel and Calophyllum inophyllum biodiesel blend (B20) at different compression ratios on the engine's performance, combustion, and emission characteristics. A single-cylinder, 4-stroke, variable compression ratio engine operating at 1500 rpm was used for the research. A standard compression ratio (SCR) of 17.5 and a modified compression ratio (MCR) of 15.5 at a constant hydrogen flow rate of 6 L per minute (lpm) were used with diesel and B 20. The addition of hydrogen with diesel and B20 resulted in higher cylinder pressure and heat release rate when compared to pure diesel and B20 operation. This resulted in a shorter delay period (by an average of 9.4 % and 7.1 %) with lower peak pressure than pure diesel and B20 operation. During the dual fuel mode of H2 with B 20 at a modified compression ratio, the maximum heat release rate was decreased and its occurrence was retarded. NOx, UBHC, and CO emissions of the engine were decreased significantly by an average of 20.8% 43.7, %, and 22 % respectively with 37.7 % increase in smoke opacity. The increase in NOx emission with the use of H2 with diesel was reduced by operating H2 with B20 and at a reduced compression ratio. This lower emission was achieved with a compromise of a marginal decrease in engine brake thermal efficiency.