Biocomposite Films Research Articles

Development and characterization of peppermint essential oil/rice husk fibre/ corn starch active biocomposite film and its performance on bread preservation

Active packaging with antimicrobial characteristics can inhibit bacterial growth and prolong the shelf life of packaged food products. Numerous attempts have been made to develop biobased eco-friendly polymer packaging films to minimize the utilization of synthetic antimicrobial packaging. However, polymer-based packaging has limited physio-mechanical properties, making it a poor candidate for packaging applications. In this study, an antimicrobial fibre-reinforced biocomposite was fabricated using rice husk fibre (RH) in the starch matrix to enhance the properties of packaging film. Peppermint essential oil (PEO), extracted from peppermint leaves, was incorporated into the film to enable antimicrobial properties. Rice husk fibre was varied from 10% to 50%, PEO ranged from 1% to 6%, and influence on various properties of biocomposite film was analysed. Developed biocomposite films were evaluated using XRD, FT-IR, SEM, water contact angle, and surface roughness. Furthermore, mechanical properties, water solubility, moisture content, colour properties, and optical barrier properties of biocomposite film were also calculated. In mechanical properties, biocomposite film that has 10% rice husk fibres, and 1% PEO showed the maximum tensile strength and elastic modulus of 2.25 MPa, and 57.4 MPa, respectively. In the biodegradability test, the degradation rate was reduced with reinforcing rice husk fibres and incorporation of PEO. The fabricated biocomposite films exhibit a zone of inhibition against Gram-positive bacteria (S. aureus and B. subtilis) as well as Gram-negative bacteria (E. coli and K. pneumoniae). Moreover, fabricated biocomposite was used as bread packaging and compared with synthetic and open packaging for shelf-life analysis. In observation, it was found that it has increased the shelf life of packed bread and reduced the growth of food-borne microbes in bread. This fabricated antimicrobial biocomposite is an eco-friendly packaging film that may reduce the food wastage and environmental impact of packaging materials by utilizing agricultural waste.

THE APPLICATION OF RICE STRAW-BASED PLA/NR BIOCOMPOSITE AS GREEN PLANTING BAGS

This study’s primary objective was to observe the growth of marigold seedling in biodegradable planting bags made from polylactic acid (PLA), natural rubber (NR), and rice straw (RS) film, as well as the biodegradation of the bags during planting. The flexible PLA/NR/RS film was produced using a PLA/NR blend ratio of 70:30 percent by weight (wt%) as a matrix and filled with RS at 3, 5, and 10 wt%. All PLA/NR/RS biocomposite films were produced via an extrusion casting method. To examine the growth efficiency of marigold seedlings in biodegradable planting bags, marigold seedlings were transferred into PLA/NR/RS biocomposite bags. After being planted for 90-days, the stalk diameter and dry mass of the marigold seedlings grown in the PLA/NR/RS biocomposite bags tended to increase with increasing RS content. The biodegradability of all PLA/NR/RS biocomposite bags was measured by how much weight each bag lost after marigold seedlings were planted in it. The amount of rice straw in PLA/NR/RS biocomposite bags was found to affect how much weight they lost. Maximum weight loss was observed when the film contained 10 wt% RS fiber, which corresponded to the lowest PLA molecular weight assessed by gel permeation chromatography (GPC).

Mechanical, UV protection, and antibacterial properties of poly(vinyl alcohol)/marine mucilage biocomposite films

AbstractThe mucilage that accumulated in the Sea of Marmara in 2021 turned into an environmental disaster that threatened not only marine life but also the shipping and tourism industries. However, because of the partially organic structure of marine mucilage, it has the potential to be used as an additive for polymer films. In this study, bioplastic films were prepared from PVA with a series of mucilage content from 0% to 50% by mass, and their properties were investigated. The FT‐IR spectra revealed a shift in hydroxyl peaks, suggesting an interaction between the mucilage and PVA. SEM images showed the formation of crystal forms at the higher mucilage content of 20%, indicating ionic saturation of the PVA matrix. EDX and XRD analyses revealed that the crystal was NaCl coming from the mucilage composition. The tensile strength of the neat PVA film was measured as 9.86 MPa and improved to 14.56 MPa with 5% mucilage content, which is attributed to the ionic cross‐linking. The tensile properties were preserved up to 20% of the mucilage content, and then sudden decrements were observed. The transmittance percentage (T%) of the neat PVA film in UV–Vis spectra (800–200 nm) was detected as 75% at 500 nm and decreased down to 39% with increased mucilage content up to 50%. Furthermore, a rising limit of linearity (LOL) of the spectrums was observed from 350 to 580 nm, with increasing mucilage content from 5% to 50%. The biofilms showed antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria. Hence, a robust and biodegradable film having UV protection and antibacterial activity was developed from the marine mucilage bio‐waste.Highlights Poly(vinyl alcohol)/marine mucilage biocomposite films were prepared. Biocomposite films showed increased UV‐A, UV‐B, and UV‐C protection with increased mucilage content. Enhanced tensile strength of biocomposite films up to 20 wt% mucilage content was determined due to ionic crosslink formed by NaCl salt ions. Biocomposite exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus.

Development of multifunctional bioactive food packaging based on silver nanoparticles/grape fruit peel extract reinforced PVA composites

Active food packaging based on bio-wastes has gained considerable attention these days due to their ecofriendly and biodegradable behavior. In this study, multifunctional bio-composite films with remarkable anti-oxidant and anti-microbial attributes were synthesized by incorporating biosynthesized silver nanoparticles (AgNPs) and grape fruit peel extract (GFPE) into polyvinyl alcohol (PVA) matrix. Synthesized films were characterized in terms of Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), Differential scanning calorimetry (DSC) and Dynamic Mechanical Analyzer (DMA). Antioxidant activity of AgNPs-GFPE/PVA films was determined by %DPPH scavenging ability and Ferric reducing antioxidant power assay while antimicrobial activity was measured by agar diffusion method. XRD analysis revealed the semi-crystalline behavior of synthesized composites with characteristics peak at 38° whereas SEM micrographs confirmed the homogeneous dispersion of reinforcement and porous rough surface structure of AgNPs-GFPE/PVA nano-composites. AgNPs-GFPE/PVA nanocomposite exhibited glass transition temperature (Tg) at ∼116 °C and Tm ∼191 °C along with the storage modulus of 685 MPa, revealing significantly enhanced thermal and mechanical stability of composites compared to pure PVA films. The composite films exhibited remarkable antioxidant potential i.e DPPH scavenging activity ∼50 % and reducing power ∼ 0.58. The AgNPs-GFPE/PVA nano-composite films exhibited remarkable antimicrobial activity in response to food borne pathogens. The results designated that the synthesized composite films have prodigious potential of being cheap, eco-friendlier substitute to conventional materials for application in food packaging, which certify the safety and economical life span of packed food.

Cellulose fortified bio-composite film preparation using starch isolated from waste avocado seed: starch properties and film performance

Cellulose fortified bio-composite film preparation using starch isolated from waste avocado seed: starch properties and film performance

Development and Characterization of a Polylactic Acid/Sesame Husk Powder–Based Biocomposite Film for Packaging Application

Development and Characterization of a Polylactic Acid/Sesame Husk Powder–Based Biocomposite Film for Packaging Application

Chitosan/Clay biocomposite films with enhanced physico-chemical, mechanical and antimicrobial properties

Chitosan (CS) is a biopolymer known for its outstanding biocompatibility, biodegradability, and antibacterial properties. On the other hand, Clays are natural inorganic materials and have good rheological and thermal properties. Therefore, it is expected that the biocomposite prepared from both these materials may have numerous intriguing properties. In this work, we report the preparation of chitosan (CS)/bentonite (BNTN) and chitosan (CS)/silica (Si) biocomposite films and characterizations of those via various analytical techniques such as X-ray diffraction (XRD), Scanning Electron Microscope (SEM), Fourier Transform Infrared (FT-IR) Spectroscopy, Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Ultraviolet/Visible (UV/Vis) Spectroscopy and Universal Testing Machine (UTM). Swelling tests of prepared biocomposite films were also carried out in distilled water to evaluate their property towards packaging applications. The synthesized biocomposite films exhibit appreciable antimicrobial activity against Escherichia coli and Bacillus subtilis bacteria using the agar well diffusion method. Additionally, it has been noted that compared to the films of the other two biocomposites, the CS/BNTN biocomposite films showed greater tensile strength, swelling capacity and antimicrobial activity.

Preparation and characterization of potato starch-based composite films reinforced by modified banana fibers and its application in packaging of grapes

The current study focuses on the preparation and characterization of potato starch-based biocomposite films by reinforcing them with banana fiber. The banana fibers were modified using ultrasonication and cellulase enzyme, individually and in combination. Both native and modified banana fibers underwent physical, morphological, FTIR, and crystallinity analyses. The resulting biocomposite films, created by incorporating native and treated banana fibers, were then evaluated for their mechanical, thermal, barrier, and biodegradable properties. The findings indicated that combining ultrasound with enzyme treatment of banana fibers in the potato starch matrix led to a substantial reduction in water-sorption and water-vapor permeability (0.156 g mm m−2 h−1 kPa−1) of the packaging films. Additionally, the mechanical properties (5.02 MPa-Tensile strength, 4.27 MPa-Sealability) of the films significantly improved with the inclusion of modified banana fibers. FTIR analysis revealed similar spectra for all modified samples, along with enhanced crystallinity. Moreover, the thermal stability of the developed films was enhanced by the incorporation of modified banana fibers. Scanning electron microscopy showed that the modified fibers exhibited smooth surfaces and an even distribution of spaces compared with the native fibers. The biocomposite films demonstrated biodegradation within 42 days. Furthermore, the packaging application was tested with grapes, which showed that the films could maintain storability for up to 8 days. Overall, these results suggest a promising eco-friendly method for producing packaging films with biocompatible, biodegradable, and non-toxic properties.

Development and Characterization of Sugar Palm (Arenga Pinnata (Wurmb. Merr)) Fiber Reinforced Cassava (Manihot esculenta) Starch Biopolymer Composites

ABSTRACT The study aims to develop a biopolymer composite made of sugar palm fiber and cassava starch that may degrade over time and to investigate the physical and structural characteristics of biocomposite films. Sugar palm fiber (SPF) was cast into a biocomposite with cassava starch (CS) as the matrix and fructose as the plasticizer using the casting process. The thermoplastic CS composite sheet was loaded with different amounts of SPF (0%, 5%, 10%, 15%, and 20% dry starch). The addition of SPF considerably improved the physical and thermal properties. It enhanced the thickness of the material while lowering its density, water content, and biodegradability. However, there was no significant increase in the tensile properties of the composite films. The filler had been absorbed into the matrix, according to SEM micrographs. With a higher concentration of SPF, the SPF/CS films had a more heterogeneous surface. It is indeed possible that the addition of SPF changed the film characteristics of cassava starch, potentially compromising the performance of the films. Overall, the findings of this study concentrate on enlightening about biopolymer composite film and highlighting its great potential for the food packaging market.

Effects of filler type and content on mechanical, thermal, and physical properties of carrageenan biocomposite films

This study investigates the influence of various fillers on the properties of carrageenan, a natural polymer derived from red seaweed. Despite its potential for enhanced biocomposite film development, carrageenan faces challenges related to strength. The incorporation of nanoclay into the carrageenan film resulted in a significant increase in film thickness from 0.026 to 0.068 mm. The UV light transmission value for the carrageenan film alone was measured at 30.9 %, whereas films containing 5 wt% of Tetraethyl orthosilicate (TEOS), 3-Aminopropyltriethoxysilane (APTES), and nanoclay exhibited reduced transmission values of 23 %, 18 %, and 1 %, respectively. Notably, the tensile strength of the unfilled carrageenan film was 38.4 MPa, which increased to 38.6, 57, and 60 MPa upon the addition of 3 wt% of nanoclay, APTES, and TEOS fillers, respectively. All fillers contributed to improved tensile strength, with TEOS demonstrating the highest enhancement. The optimal filler content was determined to be 3 wt%. Regarding thermal properties, films containing TEOS displayed higher thermal stability compared to those with APTES, while films incorporating nanoclay exhibited the lowest stability. Findings provide insights into the effects of different fillers on the mechanical, physical and thermal properties of carrageenan films, supporting the development of improved biocomposite materials suitable for application in food packaging.

Green interconnected network structure of chitosan-microcrystalline cellulose-lignin biopolymer film for active packaging applications

As an excellent alternative to petroleum-based food packaging materials, a novel green hybrid composite film with an excellent interconnected network structure was successfully fabricated by integrating chitosan (chi), microcrystalline cellulose (MCC), and lignin nanoparticles (LNP), including the desired amount of plasticizer glycerol (gly). Overall, 36 combinations were developed and investigated for superior biocomposite film formation. Among the various concentration ratios, the 40:35:25 chi-MCC-gly film provided well-organized film formation, good physicochemical properties, mechanical stability, efficient water contact angle, reduced water solubility, and lower water vapor permeability (11.43 ± 0.55 × 10−11 g.m−1.s−1.Pa−1). The performance of the chi-MCC-gly film further enhanced by the homogeneous incorporation of ∼100 nm LNP. With 1 % LNP addition, the tensile strength of the film increased (28.09 MPa, 47.10 % increase) and the water vapor permeability reached a minimum of 11.43 × 10−11 g.m−1.s−1.Pa−1, which proved the impact of LNP in composite films. Moreover, the films showed excellent resistance to thermal shrinkage even at 100 °C and exhibited nearly 100 % UV blocking efficiency at higher LNP concentrations. Interestingly, the green composite films extended the shelf life of freshly cut cherry tomatoes to seven days without spoilage. Overall, the facile synthesis of strong, insoluble, UV-blocking, and thermally stable green composite films realized for food packaging applications.

Biocomposite films from banana flour/halloysite nanoclay/carvacrol: Preparation, characterization, and application on capsicum (Capsicum annuum) fruits

In this work, banana flour (BF)-based composite films were formulated by loading halloysite nanoparticles (HS) and essential oil carvacrol (CR). HS nanoclay was selected since it has abilities to improve the mechanical and water vapor barrier properties of the prepared films. CR was used since it demonstrates powerful antimicrobial activity against foodborne pathogens. Results reveal successful incorporation of HS and CR in the composite films that display an improved melting temperature (Tm) and rough surface. The water solubility of the films significantly reduced whereas the contact angle significantly improved with the impregnation of both HS and CR. The XRD diffractograms indicated pre-dominant amorphous characteristics of all the prepared films. These changes reflected well on the mechanical properties, wherein the tensile strength and Elastic modulus increased by ∼47% and ∼206%, respectively. The presence of HS and CR also reduced the films' water vapor transmission rate by about 62%. In addition, as a case study, BF/HS/CR dispersions were coated on a capsicum fruit to test their protecting ability. The incorporation of HS and CR in the BF-based biopolymeric matrix resulted in a significant reduction in the %weight loss, from 50.96 ± 2.40% (control: without coating) to 37.42 ± 3.75% (with the coating), along with an improvement in the firmness (N) from 5.04 ± 0.92 N (control: without coating) to 7.47 ± 0.25 N (with the coating), during 12 days of storage. The outcome promises BF/HS/CR based films and dispersions as natural food packaging materials to extend the shelf life of food systems.

The impact of esterified nanofibrillated cellulose content on the properties of thermoplastic starch/PBAT biocomposite films through ball-milling

To enhance the mechanical properties and interfacial compatibility of thermoplastic starch (TPS) highly filled poly(butylene adipate co-terephthalate) (PBAT) composite films, esterified NFC was innovatively fabricated and introduced into the composite system. The influences of NFC content and ball-milling treatment were thoroughly investigated. Interestingly, the amphiphilic esterified NFC provided a “bridge-like” effect between TPS and PBAT interfaces, which significantly improved the interfacial compatibility and mechanical properties. Notably, the tensile properties of the composite films reached their maximums at a 7 wt% NFC content, displaying a tensile strength of 6.2 MPa and an elastic modulus of 263 MPa. These values corresponded to a 59 % and 180 % increase, respectively, compared to the composition without NFC. More importantly, ball-milling contributed to uniform dispersion and surface activation of NFC, preventing starch retrogradation, and enhancing the tensile strength and elastic modulus by 30.3 % and 56.6 %, respectively. Additionally, the film exhibited excellent UV-blocking, foldable, writable, and transparent performance. These findings provide valuable data supporting the expanded applications of starch-based composite films.

Synthesis, characterization, and in-vivo application of non-thermal plasma-modified talipot starch-Aloe vera gel biocomposite films for enhancing the shelf life of guava fruits

Modification of talipot starch by non-thermal plasma (NTP) treatment and addition of Aloe vera (AV) gel enhanced the crosslinking of filmogenic solution and improved biofilm properties. Both, NTP-modified talipot starch film (MTSF) and AV gel-incorporated modified talipot starch film (AV-MTSF) showed surface protuberances and roughness compared to native talipot starch film (NTSF) and AV gel-incorporated talipot starch film (AV-NTSF). The incorporation of AV gel increased the relative crystallinity and also altered the moisture content, thickness, and opacity of the films. AV-NTSF and AV-MTSF showed decreased water vapor and oxygen permeability, solubility, and elongation at break, and increased tensile strength compared to NTSF and MTSF. Furthermore, AV gel-incorporated films with comparatively good barrier properties showed enhanced control over the increase in pH and total soluble solids, reduced weight loss, firmness, titratable acidity, and ascorbic acid upon in-vivo guava coating application, which induced protectiveness against the desiccation and deterioration of guava fruits.

Polysaccharides based biopolymers for biomedical applications: A review

AbstractPolysaccharides are a class of natural biopolymers that have attracted significant attention due to their unique properties, which make them attractive for use in a wide range of applications. These biopolymers have an important role in our daily life, and designing biomaterials for biomedical applications requires careful consideration of factors such as biocompatibility, biodegradability, renewability, affordability, and availability, all of which can be achieved with polysaccharides. This review paper focuses on the use of polysaccharides in biomedical applications and provides an in‐depth analysis of the potential applications of arabinoxylan, a type of hemicellulose, in various fields, including drug delivery, infection control, tissue engineering, wound healing, and others. Arabinoxylan, which is derived from plants, is the most abundant and important class of hemicellulose and has a diverse role as a catalyst due to its biodegradability, biocompatibility, and renewable nature. Polysaccharide‐based biopolymers have applications in tissue engineering, wound covers, drug delivery systems, food preservation, and biocomposite films, making them an emerging excipient in pharmaceutical research. This review paper highlights the potential of polysaccharide‐based biopolymers in biomedical science and suggests that continued research and development will lead to broader and more innovative applications in the field. The use of polysaccharide‐based biopolymers has the potential to significantly advance healthcare and improve patient outcomes.

Ionic liquid, ultrasound-assisted synthesis of lignin nanoparticles for barrier-enhanced all-cellulose nanocomposite films

The primary purpose of this work was to develop novel all-cellulose nanocomposite (ACNC) films by following a green approach that uses an ionic liquid as solvent and requires no additional reducing agents or stabilizers. In this way, biodegradable, UV-blocking ACNC films were obtained by partially dissolving cellulose to entrap lignin nanoparticles (LNP) within. Lignin particles in proportions of 3–7 wt% were modified by sonication in an ionic liquid (IL) to obtain lignin-IL dispersions. The influence of the LNP-IL medium on the chemical, physical, and morphological properties of the resulting nanocomposites was examined, and the properties compared with those of an all-cellulose composite (ACC) film and untreated paper. The TEM technique revealed the formation of unevenly spherical LNPs as small as 5.133 ± 0.003 nm, and XRD spectroscopy a transition from cellulose I to II and an increase in the proportion of non-crystalline cellulose as a result of partial dissolution and regeneration. In addition, SEM images confirmed the deposition of LNPs onto the surface of the regenerated cellulose matrix. The incorporation of LNPs considerably enhanced the UV-blocking, oxygen and water-barrier, biodegradation, antioxidant and antibacterial properties of the films. Moreover, LNPs in proportions of 3 and 5 wt% increased tensile strength, and a proportion of 7 wt% allowed the films to block 97% of light at 280 nm and increased antioxidant activity by 68% relative to the control sample. Interestingly, a 7 wt% LNP content in the films decreased antibacterial activity against Staphylococcus aureus and Escherichia coli by about 42.85% and 63.88%, respectively. The new, multifunctional biocomposite films are suitable for various uses in cellulose-based food packaging.

Structure-property relationship in edible pectin-alginate/orange peel biocomposite films

Biodegradable plastics can help tackle plastic packaging waste, which cause serious consequences for the environment, however, current bio-based materials often lack strength, have poor thermal and barrier properties, and are expensive. This study proposes a solution: biodegradable, edible pectin-alginate films reinforced by abundant and inexpensive orange peel powder (OPP). This study stands out for its comprehensive analysis of mechanical, thermal, barrier, morphological, and biodegradable properties by adjusting the type, amount, and size of OPP in the formulation. The resulting fully biodegradable films with 2% CaCl2, 0.7 g/g glycerol, and 30% finely ground OPP not only matched the quality of commercial packaging films in mechanical properties, but also exhibited promising qualities in terms of thermal, morphological, and water barrier properties for packaging applications. Made from natural, edible, and vegan materials, these films offer an eco-friendly alternative to non-biodegradable plastics, enhancing biocomposite properties and also providing a sustainable solution to organic waste.

Fabrication of Graphene Oxide Reinforced Biocomposite: Recycling of Postconsumed Footwear Leather

The increasing concerns about solid waste disposal have led to the development of innovative strategies for repurposing waste materials. This paper describes a simple solution casting process for recycling postconsumed footwear leather fiber (PCF) into a biocomposite film reinforced with graphene oxide (GO) and polyvinylpyrrolidone (PVP). PVP was utilized as a compatibilizer to strengthen the interfacial bonding of GO and leather fiber via π–π interactions. UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and scanning electron microscopy were used to examine the material dispersibility bonding between GO and PCF, structural properties, thermal properties, and surface morphology of the biocomposite films, respectively. Compared to pure PCF film, the oxygen transmission rate of the prepared biocomposite films is elevated by 64% as well as the biodegradability rate is intensified up to 60%. In addition, the film’s tensile strengths are raised by 216%, while their elongation at break is increased by 164.64% as compared with PCF. The versatility of these eco-friendly and biodegradable composite films extends to its possible applications in packaging and interior design. The outcomes of the research reveal the viability of manufacturing affordable and sustainable biocomposites through the utilization of waste leather from consumed footwear.

Carboxymethyl chitosan-gelatin-mesoporous silica nanoparticles containing Myrtus communis L. extract as a novel transparent film wound dressing

Wound healing and health care requirements have attracted more attention, and the need to develop new drug-containing dressings to accelerate wound healing is required. Carboxymethyl chitosan (CMCS)/gelatin-based films with mesoporous silica nanoparticles (MSNs) containing the Myrtus communis L. (Myrtle) aqueous extract were designed to answer this demand. Myrtle aqueous extract included total phenolic content and good free radical scavenging ability in vitro assay. The infrared spectroscopy characterized the functional groups of myrtle extract and biocomposite films. It was found that mesoporous silica nanoparticles increased the tensile strength of the flexible dressings, which is essential in therapeutic uses. MSNs influenced swelling ratio, oxygen, and water vapor permeability that indicates the CMCS/Gelatin/Myrtle/5 % MSNs wound dressing can absorb wound exudates and preserve skin moisture. Also, these biocompatible nanoparticles reduced the cytotoxicity of fibroblast cells due to the decelerated drug release. Correspondingly, silica nanoparticles affected the extract release rate and could accumulate and release the extract prolonged in CMCS/Gelatin/Myrtle/5 % MSNs models. Finally, histological analysis showed collagen growth and fibroblast migration in wounds treated with CMCS/Gelatin/Myrtle/5 % MSNs, causing proper wound contraction and accelerating wound healing in mice models. The results suggest that CMCS/Gelatin/Myrtle/5 % MSNs films have a beneficial application as wound dressings.

Preparation and Characterization of Microcrystalline Cellulose/Polylactic Acid Biocomposite Films and Its Application in Lanzhou Lily (Lilium davidii var. unicolor) Bulbs Preservation

Green biodegradable bio-based films have gained interest in replacing petroleum-derived plastic packaging materials as a new preservation strategy for fruits and vegetables to alleviate environmental pressures. In this study, we aimed to develop novel biodegradable composite films based on microcrystalline cellulose (MCC) reinforced polylactic acid (PLA). Our results demonstrated that the addition of 3% MCC to PLA could improve its tensile strength. Scanning electron microscopy analysis revealed that MCC dispersed well in PLA at lower content while agglomerated at higher content. It was discovered that all four types of MCC/PLA biocomposite films could retard the color change of Lanzhou lily bulbs, accompanied by maintaining favorable total soluble solid, total sugar, total polyphenols, and flavonoid content, inhibiting the activities of phenylalanine ammonia-lyase and the content of malondialdehyde during storage. Moreover, the preservation effect of MCC/PLA biocomposite films on Lanzhou lily bulbs was evaluated using a membership function, and the SSS MCC/PLA biocomposite film demonstrated a favorable fresh-keeping effect. In conclusion, four types of MCC from different biomass materials added to PLA-based products can be beneficial in improving the attractive properties of biocomposite films. These films are expected to replace petroleum-derived plastics as a new packaging material for preserving Lanzhou lily bulbs.