Abstract Bat population changes are poorly understood, making it difficult to establish management priorities. In 2017, a summer mist-netting survey previously conducted in 2004 at Fort Indiantown Gap was replicated to evaluate temporal changes in the local bat community. The authors hypothesized that captures of species most affected by white-nose syndrome (WNS) would decline between survey periods, and that community structure would shift. The results showed a 100% decline in captures of northern long-eared Myotis (Myotis septentrionalis) and little brown Myotis (M. lucifugus), no change in captures of eastern red bats (Lasiurus borealis), a 276% increase in captures of big brown bats (Eptesicus fuscus), and a considerable shift in community structure. These results are consistent with expected effects of WNS on Myotis populations. Increases in big brown bat populations may result from ecological release after the decline of Myotis species, or from local or large-scale habitat change over time.