Berry Extracts Research Articles

In Vitro Inhibitory Potential of Different Anthocyanin-Rich Berry Extracts in Murine CT26 Colon Cancer Cells

Anti-oxidant, -inflammatory, and -carcinogenic activities of bioactive plant constituents, such as anthocyanins, have been widely discussed in literature. However, the potential interaction of anthocyanin-rich extracts with routinely used chemotherapeutics is still not fully elucidated. In the present study, anthocyanin-rich polyphenol extracts of blackberry (BB), bilberry (Bil), black currant (BC), elderberry (EB), and their respective main anthocyanins (cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, cyanidin-3-O-rutinoside, and cyanidin-3-O-sambubioside) were investigated concerning their cytotoxic and DNA-damaging properties in murine CT26 cells either alone or in combination with the chemotherapeutic agent SN-38. BB exerted potent cytotoxic effects, while Bil, BC, and EB only had marginal effects on cell viability. Single anthocyanins comprised of the extracts could not induce comparable effects. Even though the BB extract further pronounced SN-38-induced cytotoxicity and inhibited cell adhesion at 100–200 µg/mL, no effect on DNA damage was observed. In conclusion, anti-carcinogenic properties of the extracts on CT26 cells could be ranked BB >> BC ≥ Bil ≈ EB. Mechanisms underlying the potent cytotoxic effects are still to be elucidated since the induction of DNA damage does not play a role.

CURRENT TRENDS IN THE APPLICATION OF VACCINIUM MYRTILLUS AND ARONIA MELANOCARPA AS NATURAL PRESERVATIVES IN MEAT AND MEAT PRODUCTS: A REVIEW

"In the contemporary food industry, preservation of meat and meat products remains a paramount concern for ensuring safety, quality, and extended shelf life. Against the backdrop of consumer demand for natural and clean-label food additives, this comprehensive review investigates the current trends in utilizing Vaccinium myrtillus (bilberry) and Aronia melanocarpa (chokeberry) extracts as natural preservatives in meat and meat products. The review begins by elucidating the intrinsic antioxidant and antimicrobial properties of these berry extracts, emphasizing their relevance in the preservation of meat products. Recent research highlights their efficacy in reducing lipid oxidation, extending the shelf life of meat products, and inhibiting microbial proliferation. Furthermore, it explores various application methods, including their incorporation into meat matrices and the development of edible coatings, shedding light on innovative techniques and emerging technologies. The practicality of deploying these natural preservatives in meat processing is substantiated through industrial applications and illustrative case studies. Recognizing the promise of these natural preservatives, the review acknowledges the hurdles faced, including formulation optimization, cost-effectiveness, and consumer perceptions. Overcoming these challenges is crucial for their widespread adoption. The review concludes by summarizing key findings and underscores the pivotal role of Vaccinium myrtillus and Aronia melanocarpa in shaping the landscape of meat preservation while ensuring consumer safety and satisfaction."

Evaluation of the therapeutic efficacy of Vitex agnus-castus extract on cisplatin-induced hematotoxicity in female Wistar rats.

Cisplatin (CP) is a preferred drug for cancer treatment but it has dose-dependent side effects. Vitex agnus-castus (VAC) berry extract has antioxidant, free-radical scavenging, and anti-inflammatory activities. This study explored the mitigating effects of VAC extract (VACE) on acute hematotoxicity induced by CP in female Wistar rats. Female Wistar rats (n = 30) were randomly divided into five groups (n = 6/group). The normal control (NC) group received no treatment. The CP control group received CP (7 mg/kg.b.w. ip, single dose) and the drug control group (VACE-650) received VACE (650 mg/kg b.w. oral, daily) for 7 days. Both groups received a single dose of CP (7 mg/kg b.w. ip), followed by 350 and 650 mg/kg.b.w. of VACE daily orally (CPVACE-350 and CPVACE-650 groups, respectively) for 7 days. After a single dose of CP (7 mg/kg b.w.), the red blood cells (RBC), hematocrit (HCT), white blood cells (WBC), and platelets significantly decreased. In the VAC-350 group, the reduction in total WBC count was less than that in the VAC-650 group on the 3rd day. The RBC and HCT values of the VACE groups were better than that of the CP control, but the VACE-350 treatment group showed significant improvement only on the 3rd day. Our findings showed that VACE can mitigate CP-induced damage to peripheral blood cells at lower doses.

Antioxidant and Geroprotective Properties of the Extract of Mountain Ash (<i>Sorbus aucuparia</i> L.) Fruits

Plant polyphenols are characterized by a wide range of biological activities, including antioxidant properties, and have a high geroprotective potential. The purpose of this work was to investigate the effect of the extract of rowan berries (Sorbus aucuparia L.) on the lifespan and stress resistance of Drosophila melanogaster with the identification of possible mechanisms of its biological activity. It has been established that the ethanol extract of S. aucuparia berries, the main components of which are rutin and cyanidin-3-rutinoside, has a pronounced antioxidant activity in vitro. At the same time, treatment with rowan berry extract increased the r-esistance of D. melanogaster males to starvation, but reduced resistance to hyperthermia. In females, the e-xtract reduced resistance to oxidative stress but increased resistance to hyperthermia. The effects of rowan berry extract on longevity depended both on its concentration and on the sex of fruit flies. In response to treatment with rowan berry extract, D. melanogaster males and females showed slight differences in the background level of expression of cellular stress response genes, including heat shock genes (hsp27, hsp68, hsp83), oxidative stress resistance genes (hif1, nrf2, sod1), circadian rhythm genes (clk, per), and the longevity gene sirt1, which may explain the differences in the observed effects.

Chemical Profiling of Nitraria roborowskii Kom. by UPLC-Q-Orbitrap-MS and Their Hypolipidemic Effects in Vivo.

The Nitraria roborowskii Kom. (NRK) berries, as fruits of the genus Nitraria of the Zygophyllceae family, have been widely used as folk medicine. Modern pharmacological research has demonstrated that Nitraria berries had hypolipidemic, hypoglycemic, and immunomodulatory effects. However, more research needs to be reported on the chemical composition and biological activity of NRK. Hence, the phenolic compounds in the NRK berries were comprehensively analyzed and characterized by Ultra Performance Liquid Chromatography-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS) in this study. In total, 52 phenolics were identified, and all were reported for the first time. In addition, the hypolipidemic efficacy of NRK berries extract was studied in the hyperlipidemic mouse model. After treatment, the high dose group of NRK substantially reversed total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels. Through lipidomics technology, 27 potential biomarkers were characterized. And there was a significant callback at 25 of them after NRK treatment by using statistical analysis methods. Pathway analysis results demonstrated that NRK might exert therapeutic effects by regulating glycerophospholipid and glycerolipid metabolism pathways. This study could provide firsthand information on NRK berries for their phenolic compounds and potential application in preventing and treating hyperlipidemia.

Flavonoid-Rich Sambucus nigra Berry Extract Enhances Nrf2/HO-1 Signaling Pathway Activation and Exerts Antiulcerative Effects In Vivo.

Sambucus nigra (SN) berry extract is characterized by high antioxidant and anti-inflammatory activity. The current study aimed to investigate the effect of SN berry extract against indomethacin (IND)-induced gastric ulcer in rats and the mechanism involved. SN berry extract alleviated IND-induced gastric ulcers, as shown by assessing pathological manifestations in the gastric mucosa. These protective effects are attributed to attenuated oxidative damage to the gastric mucosa, correlated to increased activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), enhanced glutathione (GSH) levels, total antioxidant capacity (TAC), and upregulation of the Nrf2/HO-1 cascade. Moreover, oxidative stress markers, including malondialdehyde (MDA) and total oxidant status (TOS), were downregulated in SN-extract-treated animals. Furthermore, SN berry extract suppressed gastric mucosal inflammation by downregulating interleukin (IL)-33, IL-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) levels, and attenuating myeloperoxidase (MPO) activity. The protective effects of SN berry extract were similar to those exerted by esomeprazole (ESO), an acid-secretion-suppressive drug. In conclusion, SN berry extract has antiulcerative effects, alleviating oxidative stress and inflammation.

Three anthocyanin-rich berry extracts regulate the in vitro digestibility of corn starch: Physicochemical properties, structure and α-amylase

This study aimed to compare the regulatory effects of blue honeysuckle anthocyanins (BHA), blueberry anthocyanins (BBA), and blackcurrant anthocyanins (BCA) on the in vitro digestibility of corn starch in terms of starch physicochemical properties and structure, as well as α-amylase inhibition. The results revealed that adding all three anthocyanins lowered digestibility in the following order: BHA > BCA > BBA. The terminal digestibility (C∞) decreased from 73.84 % to 57.3 % with the addition of 10 % BHA, while the resistant starch (RS) content increased from 4.39 % to 48.82 %. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis indicated that anthocyanins and starch interacted through noncovalent bonds. Differential scanning calorimetry (DSC) analysis showed that the gelatinization enthalpy was dramatically lowered in all three anthocyanin groups, with 10 % BHA producing a 38.58 % drop. Rheological property analysis showed that anthocyanins increased the apparent viscosity and modulus with starch. The interaction between anthocyanin and α-amylase was mainly through the formation of hydrogen bonds and hydrophobic forces. This research provides theoretical guidance for developing low glycemic index (GI) anthocyanin starch-based foods.

Fruit Smoothies Enriched in a Honeysuckle Berry Extract-An Innovative Product with Health-Promoting Properties.

Smoothies are claimed to be an effective way of promoting fruit and vegetable consumption. They are a rich source of bioactive compounds and provide numerous health benefits. Strawberries and apples are among the most popular smoothie ingredients. Additionally, chokeberry presents antibacterial, antiviral and anti-inflammatory properties. Another interesting fruit with a wide range of health benefits is the honeysuckle berry. In this study, a dry extract from the mentioned fruit was combined to produce a smoothie enriched in bioactive compounds of unique health-promoting properties. The smoothies were rich in anthocyanins, flavonols, phenolic acids, flavan-3-ols and iridoids. Smoothies with higher concentrations of a polyphenol-iridoid honeysuckle berry extract (0.50%) were the products of a greater content of bioactive compounds and higher antioxidant activity compared to those with no extract or a lower amount (0.25%). However, the sensory evaluation showed that, according to customers, the least attractive smoothies are those with the greatest amounts of the honeysuckle berry extract. Therefore, the correct balance between taste and bioactivity should be sought in order to obtain an innovative product showing characteristics of functional food.

PB0006 Investigation of the Antiplatelet, Antioxidant and Anti-Inflammatory Potency of Berries Extracts

PB0006 Investigation of the Antiplatelet, Antioxidant and Anti-Inflammatory Potency of Berries Extracts

Technology for processing blueberries into concentrated extracts

Relevance. The production of food products using fruit and berry raw materials is one of the main directions in the creation of products with high nutritional value. The greatest prospects in this direction as ingredients are concentrated fruit and berry extracts due to the convenience of storage and the simplicity of the method of adding to products. The development of technologies for obtaining concentrated extracts of a functional orientation from fruit and berry raw materials, which ensure the safety of substances useful for the body, has recently become more and more relevant.Methods. The objects of the study were dried berries and pomace of blueberries, extracts obtained from them. As the main indicators in the objects, the content of vitamin C, bioflavonoids, tannins, organic acids, etc. was determined by generally accepted standard methods. The antioxidant activity of the extracts was determined by the kinetic method.Results. A technology for the production of extracts from fruit and berry raw materials has been developed, which ensures minimal influence of negative factors on the complex of biologically active substances of fruit and berry raw materials during processing and their high safety, which makes it possible to obtain extracts of functional orientation and high antioxidant activity.

A-047 Role of the Gut Microbiota in the Efficacy of Dietary Intervention With Flavonoid-Containing Foods in Obesity

Abstract Background Flavonoids are an active component of plant-based diets that have been investigated for their beneficial antioxidant properties in high fat diet induced obesity animal models and human intervention trials. Certain bacterial members of the gut microbiota have been reported that transform flavonoids into monophenolic acids. Our goal is to characterize the microbiome link between a flavonoid-rich diet and improved metabolic parameters characteristic of obesity. We hypothesize that flavonoid supplemented diets are most effective in presence of microbiota capable of monophenolic acids formation, and that these bacterial metabolites are a main bioactive compound responsible for the improved metabolic parameters. Methods To test our hypothesis, males and females C57BL/6 mice were fed a high fat control diet, or the same diet supplemented with 1% berry extract, or 1% monophenolic acid for 12 weeks. Separate experimental groups received either regular drinking water or antibiotic water to suppress their gut microbial community. We monitored metabolic parameters, including body weight, lean and fat mass, glucose and insulin tolerance, histological assessment of liver cell, quantification of liver injury biomarkers, and transcriptional changes in metabolically active peripheral tissues. Results For insulin tolerance test, mice on high fat diet with regular water, or antibiotic water control mice had a comparable increase in insulin resistance, demonstrated by the increase in glucose levels after injecting insulin. Similarly, the groups of mice on high fat diet with 1% monophenolic acid and regular water, or antibiotic water, had improved insulin sensitivity, presented as a decrease in glucose levels after insulin injection. Consistent with our hypothesis, mice on high fat diet supplemented with 1% berry extract had an improved insulin sensitivity, and this effect was dependent on an intact microbiome. This suggests that the gut microbiota are required to metabolize the berry extract to produce bioactive metabolites that improve insulin tolerance. We also observed a significant decrease in fasting blood glucose, an improvement in the expression for genes involved in lipid and glucose metabolism, and a noticeable reduction in hepatic steatosis in both 1% monophenolic acid and 1% berry extract with high fat diet group, compared to the high fat diet control group. Conclusion By feeding the mice a flavonoid rich diet, with the presence of an intact gut microbiota, we can improve the metabolic parameters associated with metabolic diseases. Our work will open the door for new benign approaches for treatment, such as dietary flavonoid supplementation with concomitant gut microbial probiotic therapy.

Comparative Analysis of Chemical Profile and Biological Activity of Juniperus communis L. Berry Extracts.

Researchers are looking for the most effective ways to extract the bioactive substances of Juniperus communis L. berries, which are capable of displaying the greatest range of biological activity, namely antimicrobial potential "against phytopathogens", antioxidant activity and nematocidal activity. This study provides detailed information on the chemical activity, group composition and biological activity of the extracts of juniper berries of 1- and 2-year maturity (JB1 and JB2), which were obtained by using different solvents (pentane, chloroform, acetone, methanol and 70% ethanol) under various extraction conditions (maceration and ultrasound-assisted maceration (US)). Seventy percent ethanol and acetone extracts of juniper berries were analyzed via gas chromatography-mass spectrometry, and they contained monoterpenes, sesquiterpenes, polysaccharides, steroids, fatty acid esters and bicyclic monoterpenes. The antimicrobial activity was higher in the berries of 1-year maturity, while the acetone extract obtained via ultrasound-assisted maceration was the most bioactive in relation to the phytopathogens. Depending on the extraction method and the choice of solvent, the antioxidant activity with the use of US decreased by 1.5-1.9 times compared to the extracts obtained via maceration. An analysis of the nematocidal activity showed that the sensitivity to the action of extracts in Caenorhabditis elegans was significantly higher than in Caenorhabditis briggsae, particularly for the acetone extract obtained from the juniper berries of 1-year maturity.

<i>Lonicera caerulea</i> L. Comparative metabolomics study of polyphenolic composition by tandem mass spectrometry of four varieties of Far Eastern honeysuckle

Relevance. The article presents a comparative metabolomic study of blue honeysuckle (L. caerulea) berries, varieties Volhova, Tomichka, Goluboe vereteno, Amfora, presented in the collection of the Far Eastern Experimental Station of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.Methods. To determine the complex of secondary metabolites in extracts of blue honeysuckle berries. used the method of tandem mass spectrometry. Fractional maceration was used to obtain highly concentrated extracts. In extracts of L. caerulea berries, 75 compounds of the polyphenol group were reliably identified by tandem mass spectrometry.Results. Based on the results of the qualitative identification of the component composition of blue honeysuckle berry extracts, a Venn diagram was constructed showing the similarity and difference in polyphenol groups of various L. caerulea varieties. Berries of L. caerulea contain a large amount of biologically active substances, in particular, they have a large number of compounds with antioxidant activity. The isolated polyphenol complex included compounds of the following groups: flavones, flavonols, flavan-3-ols, flavanones, anthocyanins, stilbenes, coumarins, phenolic acids. Also, for the first time in the extracts of L. caerulea berries, 30 chemical compounds of the polyphenol group were identified (flavones Pentahydroxydimethoxyflavone, Cirsiliol, jaceosidin, Sophoraisoflavone A, Dihydroxy tetramethoxyflavone, Chrysoeriol-O-hexoside, Formononetin-7-O-glucoside, flavon-3-ols (epi)-Afzelechin, (epi)-Catechin gallate, stilbenes Pinosylvin, Resveratrol, Dihydroresveratrol, coumarins Fraxetin, Umbelliferone, etc.), not previously mentioned in studies on L. caerulea.

In Vitro and In Vivo Anti-Aging Effect of Coffee Berry Nanoliposomes.

Encapsulation of bioactive compounds in the liposome system provides several advantages, such as enhancing the stability and lowering the toxicity of active compounds. Coffee berry extract (CBE) has previously been established to have in vitro anti-aging properties and to retard the aging of human skin. The purposes of this study were to encapsulate CBE in nanoliposomes and to assess its stability and in vitro anti-aging potential in human dermal fibroblasts (HDF), as well as in healthy human skin. In the HDF model, anti-aging potential was determined by nitric oxide (NO) and collagenase inhibition assays and a superoxide dismutase (SOD) activity assay, whereas in healthy human skin (in vivo), the skin elasticity and brightness were examined. First, liposomal CBE (L-CBE) was created with a particle size of 117.33 ± 2.91 nm, a polydispersity index (PDI) of 0.36 ± 0.03, and a zeta potential of -56.13 ± 1.87 mV. The percentages of encapsulation efficacy (%EE) and loading efficacy (%LE) were 71.26 ± 3.12% and 2.18 ± 0.18%, respectively. After undergoing a 12-week stability test, the L-CBE retained more phenolic content than the free CBE when stored at 4 °C, room temperature, and 45 °C. Compared to free CBE, the L-CBE demonstrated a more consistent, elevated, and prolonged release of phenolics from the lipid system. In human dermal fibroblasts, L-CBE showed lower toxicity, and at its maximum nontoxic concentration (10 mg/mL), it exhibited slightly higher anti-aging effects than CBE, including NO inhibition, enhanced SOD activity, and anti-collagenase activities. In clinical trials (30 volunteer subjects), none of the participants' skin was irritated when the L-CBE, the CBE, or base creams were applied. After 2 weeks of application, the L-CBE and CBE creams both demonstrated an improvement in skin elasticity and a reduction in melanin levels, and after 4 weeks, L-CBE cream showed a significantly greater improvement in skin elasticity and lightening. The results demonstrate that the encapsulation of the CBE in liposomal systems could increase its stability and skin penetration, reduce its toxicity, and maintain its anti-aging effect, which is powerful enough to be exploited in anti-aging and whitening agents for application in cosmetics and cosmeceuticals.

Comparative Histological Study on the Protective and Therapeutic Role of Açai Berry Extract on Experimentally Induced Fundic Gastric Mucosal Injury in Adult Male Albino Rat

Comparative Histological Study on the Protective and Therapeutic Role of Açai Berry Extract on Experimentally Induced Fundic Gastric Mucosal Injury in Adult Male Albino Rat

Picrotoximaesins B-D, new picrotoxane-type sesquiterpenoids from the berries of Maesobotrya dusenii (Pax) Hutch

The phytochemical investigation of the methanolic berry extract of Maesobotrya dusenii has led to the isolation and characterization of three novel picrotoxane-type sesquiterpenoids named picrotoximaesins B-D (2–4) alongside with five known compounds. The structures of the compounds were determined by analyses of their spectroscopic (1D and 2D NMR) and spectrometric (HRMS) data in conjunction with literature records. The relative configurations were established by the biosynthetic pathway of picrotoxane-type sesquiterpene, comparison with analogues described in the literature and by using their NOESY spectra.

진생베리 추출물 음용과 자발적운동이 항산화 및 세포사멸에 미치는 영향

진생베리 추출물 음용과 자발적운동이 항산화 및 세포사멸에 미치는 영향

Investigation into Polyphenol Profile and Biological Activities of Enriched Persimmon/Apple Smoothies during Storage.

Smoothies are becoming an increasingly popular product as a healthy alternative to snacks. The consumer expects from this product that, apart from its nutritional value, it will also be qualitatively stable during storage. Therefore, in this study, original smoothies obtained with persimmon fruit puree and apple juice (Dk/Md) enriched with Arbutus unedo fruits, Myrtus communis purple berry extract, Acca sellowiana, and Crocus sativus petal juice were evaluated for their polyphenol composition, antioxidant activity, and inhibition on targeted digestive enzymes, over six months of storage. The amount of polyphenols evaluated by UPLC-PDA analysis decreased in six months from 23.5% for both Dk/Md and enriched C. sativus smoothies to 42.5% for enriched A. sellowiana, with anthocyanins the most sensitive compounds (71.7-100% loss). Values of antioxidant assays generally strongly decreased during the first three months (up to ca. 60%) and to a lesser extent in the following three months (0.4-27%). In addition, inhibitory activity on α-amylase, α-glucosidase, and pancreatic lipase, especially on the last two enzymes, was negatively affected by time storage. The outcome of this study indicates that persimmon fruit is a good option for producing smoothies, and enrichment with other plant extracts can enhance the bioactive compound content and biological activities. It is recommended that appropriate storage strategies to preserve the properties of those smoothies should be developed.

Berry Extracts and Their Bioactive Compounds Mitigate LPS and DNFB-Mediated Dendritic Cell Activation and Induction of Antigen Specific T-Cell Effector Responses.

Berries have gained widespread recognition for their abundant natural antioxidant, anti-inflammatory, and immunomodulatory properties. However, there has been limited research conducted thus far to investigate the role of the active constituents of berries in alleviating contact hypersensitivity (CHS), the most prevalent occupational dermatological disease. Our study involved an ex vivo investigation aimed at evaluating the impact of black raspberry extract (BRB-E) and various natural compounds found in berries, such as protocatechuic acid (PCA), proanthocyanidins (PANT), ellagic acid (EA), and kaempferol (KMP), on mitigating the pathogenicity of CHS. We examined the efficacy of these natural compounds on the activation of dendritic cells (DCs) triggered by 2,4-dinitrofluorobenzene (DNFB) and lipopolysaccharide (LPS). Specifically, we measured the expression of activation markers CD40, CD80, CD83, and CD86 and the production of proinflammatory cytokines, including Interleukin (IL)-12, IL-6, TNF-α, and IL-10, to gain further insights. Potential mechanisms through which these phytochemicals could alleviate CHS were also investigated by investigating the role of phospho-ERK. Subsequently, DCs were co-cultured with T-cells specific to the OVA323-339 peptide to examine the specific T-cell effector responses resulting from these interactions. Our findings demonstrated that BRB-E, PCA, PANT, and EA, but not KMP, inhibited phosphorylation of ERK in LPS-activated DCs. At higher doses, EA significantly reduced expression of all the activation markers studied in DNFB- and LPS-stimulated DCs. All compounds tested reduced the level of IL-6 in DNFB-stimulated DCs in Flt3L as well as in GM-CSF-derived DCs. However, levels of IL-12 were reduced by all the tested compounds in LPS-stimulated Flt3L-derived BMDCs. PCA, PANT, EA, and KMP inhibited the activated DC-mediated Interferon (IFN)-γ and IL-17 production by T-cells. Interestingly, PANT, EA, and KMP significantly reduced T-cell proliferation and the associated IL-2 production. Our study provides evidence for differential effects of berry extracts and natural compounds on DNFB and LPS-activated DCs revealing potential novel approaches for mitigating CHS.

Investigation of Antioxidant and Cytotoxicity Activities of Chocolate Fortified with Muscadine Grape Pomace.

Muscadine grape pomace and mixed products with chocolate extracts from three muscadine genotypes exhibiting different berry skin colors (black and bronze) were investigated for total phenolic content (TPC), total flavonoid content (TFC), DPPH, FRAP antioxidant activity, and anticancer activity using MDA-MB-468 (MM-468; African American) breast cancer cells. Muscadine berry extracts and mixed products showed cytotoxicity activities of up to 70% against MM-468 breast cancer cells. Cell growth inhibition was higher in 'macerated Floriana' with an IC50 value of 20.70 ± 2.43 followed by 'Alachua' with an IC50 value of 22.25 ± 2.47. TPC and TFC in macerated MGP powder were (1.4 ± 0.14 and 0.45 ± 0.01 GAE/g FW, respectively), which was significantly higher than those in cocoa powder. Data analysis showed a high association between DPPH, FRAP antioxidant activities, and TPC content and a positive high correlation between anticancer activity and antioxidant capacity and between TPC and anticancer activity. The anticancer and antioxidant effects of muscadine grape pomace and chocolate extracts are attributed to the TPC of extracts, which showed a stronger positive correlation with growth inhibition of African American breast cancer cells. This study would be of great value for food industries as well as other manufacturers who are interested in new food blends.