<i>Lonicera caerulea</i> L. Comparative metabolomics study of polyphenolic composition by tandem mass spectrometry of four varieties of Far Eastern honeysuckle

Abstract

Relevance. The article presents a comparative metabolomic study of blue honeysuckle (L. caerulea) berries, varieties Volhova, Tomichka, Goluboe vereteno, Amfora, presented in the collection of the Far Eastern Experimental Station of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.Methods. To determine the complex of secondary metabolites in extracts of blue honeysuckle berries. used the method of tandem mass spectrometry. Fractional maceration was used to obtain highly concentrated extracts. In extracts of L. caerulea berries, 75 compounds of the polyphenol group were reliably identified by tandem mass spectrometry.Results. Based on the results of the qualitative identification of the component composition of blue honeysuckle berry extracts, a Venn diagram was constructed showing the similarity and difference in polyphenol groups of various L. caerulea varieties. Berries of L. caerulea contain a large amount of biologically active substances, in particular, they have a large number of compounds with antioxidant activity. The isolated polyphenol complex included compounds of the following groups: flavones, flavonols, flavan-3-ols, flavanones, anthocyanins, stilbenes, coumarins, phenolic acids. Also, for the first time in the extracts of L. caerulea berries, 30 chemical compounds of the polyphenol group were identified (flavones Pentahydroxydimethoxyflavone, Cirsiliol, jaceosidin, Sophoraisoflavone A, Dihydroxy tetramethoxyflavone, Chrysoeriol-O-hexoside, Formononetin-7-O-glucoside, flavon-3-ols (epi)-Afzelechin, (epi)-Catechin gallate, stilbenes Pinosylvin, Resveratrol, Dihydroresveratrol, coumarins Fraxetin, Umbelliferone, etc.), not previously mentioned in studies on L. caerulea.