6-benzylaminopurine Research Articles

Multiple bud clumps induction of banana cv. Barangan Merah (Musa acuminate Colla.) under in vitro treatment of benzyl amino purine and indole-3 acetic acid

Cultivar Barangan is one of the famous banana plants that has good taste and price. The disadvantages of this banana is susceptible to Fusarium disease. Tissue culture techniques is one ways of plants propagation for this plant. This study is aimed to determine the effective concentration of benzyl amino purines (BAP) and indole-3 acetic acid (IAA) for optimal multiple bud clumps (MBC) induction in explants of Barangan Merah banana. The Research was carried out at the Laboratory of Tissue Culture, Faculty of Agriculture, Universitas Syiah Kuala. The research design was a completely randomized design, 3x2 factorial. BAP concentration was the first factor, i.e. 5, 10 and 15 mg L−1. The second factor was the concentration of IAA, i.e. 0.1 and 0.5 mg L−1. The results of this research showed that 96,29% explants of all treatments survived. Explant contamination was 11,11% occurred by bacteria and brownish exudate that spread to the medium. MBC are significantly increased at 16 days after induction at concentration of BAP 5 mg L−1 + IAA 0.5 mg L−1. All combinations of BAP and IAA concentrations have produced MBC explants (100%) at eighth weeks after induction. The concentration of 5 mg L−1 BAP + 0.5 mg L−1 IAA resulted the highest shoots number. Analysis of variance and Least Significant Different further test showed that the longest of shoot length was founding the concentration of 5 mg L−1 BAP compared to other treatments. Increasing concentrations of BAP 10 mg L−1and 15 mg L−1 resulted in significantly lower shoot length. It can be concluded that taken together the concentration of 5 mg L−1 BAP + 0.5 mg L−1 IAA is the best to effectively multiple bud clumps induction in explants of Barangan Merah banana through tissue culture technique.

Effective Priming Techniques to Enhance Ghaf (Prosopis cineraria L. Druce) Seed Germination for Mass Planting

Seed priming is a cost-effective and efficient pre-sowing technique that promotes seed germination and plant growth under abiotic stress. This study was conducted to investigate the effect of different priming treatments on breaking the dormancy of ghaf (Prosopis cineraria L. Druce) seeds. Furthermore, the effects of storage conditions and seed coating on seed germination were examined. Treatments with hot water, KNO3, KCl, polyethylene glycol (PEG), H2SO4, gibberellin (GA3), cytokinin benzyl adenine (BA), and H2SO4 + BA were investigated. Among all the tested treatments, seeds responded the best to the H2SO4 50% 2 min + BA (200 ppm) priming treatment in terms of germination uniformity and effectiveness. This treatment initiated germination 5 days after sowing and increased the germination percentage from 11.32% in the untreated seed to 83% and 100% in the primed ones after 5 and 20 days, respectively. The germination percentage and primary root length were shown to be more responsive to this combined treatment, followed by the H2SO4 50% 2 min+ BA 100 ppm. This treatment achieved the best result of 100% germination when the seeds were covered in a seed ball. Additionally, the treated seeds could be stored at room temperature or 4 °C for more than 10 days with no negative effect on the germination. Based on these findings, this treatment could be recommended to farmers and plant producers to boost the mass production of ghaf trees.

In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation

Fast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of adult-selected genotypes. This study aimed to evaluate the in vitro cloning of Bambusa vulgaris in different culture systems, sucrose and activated charcoal supplementation by the inter-simple-sequence repeat (ISSR) molecular markers. In vitro bud multiplication and shoot elongation were evaluated in three cultivation systems: semi-solid and liquid culture media, and temporary immersion bioreactor (TIB). The sucrose concentrations, 0 and 30 g L−1 were evaluated in the stages. Both the culture media were supplemented with 2.0 mg L−1 benzylaminopurine (BAP) and 0.5 mg L−1 α-naphthalene acetic acid (NAA). The absence and presence of activated charcoal (100 mg L−1) were evaluated in the in vitro rooting. MS culture medium was supplemented with 2 mg L−1 indole-3-butyric acid (IBA), 1.0 mg L−1 NAA, and 0.5 mg L−1 BAP. Semi-solid culture medium supplemented with 30 g L−1 of sucrose presented superior emission of bud per explant. Liquid culture medium supplemented with 30 g L−1 of sucrose presented the most elongated shoots. Activated charcoal in the culture medium did not influence the adventitious rooting. Micropropagated plants showed genetic fidelity and were clones of the adult selected plant.

Genome-wide identification of the soybean cytokinin oxidase/dehydrogenase gene family and its diverse roles in response to multiple abiotic stress.

Cytokinin oxidase/dehydrogenase (CKX) irreversibly degrades cytokinin, regulates growth and development, and helps plants to respond to environmental stress. Although the CKX gene has been well characterized in various plants, its role in soybean remains elusive. Therefore, in this study, the evolutionary relationship, chromosomal location, gene structure, motifs, cis-regulatory elements, collinearity, and gene expression patterns of GmCKXs were analyzed using RNA-seq, quantitative real-time PCR (qRT-PCR), and bioinformatics. We identified 18 GmCKX genes from the soybean genome and grouped them into five clades, each comprising members with similar gene structures and motifs. Cis-acting elements involved in hormones, resistance, and physiological metabolism were detected in the promoter regions of GmCKXs. Synteny analysis indicated that segmental duplication events contributed to the expansion of the soybean CKX family. The expression profiling of the GmCKXs genes using qRT-PCR showed tissue-specific expression patterns. The RNA-seq analysis also indicated that GmCKXs play an important role in response to salt and drought stresses at the seedling stage. The responses of the genes to salt, drought, synthetic cytokinin 6-benzyl aminopurine (6-BA), and the auxin indole-3-acetic acid (IAA) at the germination stage were further evaluated by qRT-PCR. Specifically, the GmCKX14 gene was downregulated in the roots and the radicles at the germination stage. The hormones 6-BA and IAA repressed the expression levels of GmCKX1, GmCKX6, and GmCKX9 genes but upregulated the expression levels of GmCKX10 and GmCKX18 genes. The three abiotic stresses also decreased the zeatin content in soybean radicle but enhanced the activity of the CKX enzymes. Conversely, the 6-BA and IAA treatments enhanced the CKX enzymes' activity but reduced the zeatin content in the radicles. This study, therefore, provides a reference for the functional analysis of GmCKXs in soybean in response to abiotic stresses.

Effect of Coconut Water and Benzyl Adenine with Different Concentrations on Potato Tissue Culture (Solanum tuberosum L.) Var. Granola

This study aimed to determine the effect of giving coconut water and the right concentration of benzyl adenine in MS media for potato tissue culture of Granola variety. The study was carried out from November to January 2022, in the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Pattimura University, Ambon. This study used a 2-factor completely randomized design (CRD); the first factor was the concentration of coconut water 0 with 100 ml/l, and the second factor was the concentration of BA consisting of 0.1,2,3,4 mg/l. The study was conducted with 7 replicates and each replication consisted of 5 explants in each culture bottle, so that there were 350 cultures as the unit of observation. The variables observed in this study included the number of shoots, the number of internodes, the number of branches, and the number of roots per plantlet. The results showed that the concentration of coconut water 0 and 100 ml/l had an effect on the number of internodes and the number of roots at 4 weeks after planting. Benzyl adenine at 0, 1, 2, 3 and 4 mg/l had an effect on the variable number of shoots at 8 WAP and the number of internodes at 2 - 8 WAP; which also applies to the number of roots. Whereas, the interaction between both factors affected the number of internodes at 4, 6, and 8 weeks, which also applies to the number of roots.

Morphological and Developmental Responses of Macro Cuttings of Vernonia Amygdalina Del. to Synthetic Growth Hormones

The morphological and developmental responses of Vernonia amygdalina macro shoot cutting to plant growth hormonal solution of 6-benzyl amino purine (BAP) and naphthalene acetic acid (NAA) were investigated in the laboratory. The concentration levels of 2.5, 5.0; 7.5 and 10.0mg/l were used alongside a control treatment (0mg/i). The growth parameters examined were shoot length, root length and leaf number. The protein and carbohydrate contents of the plants were also assessed. The result of this study showed that the root length and leaf number of the species were significantly (P=0.05) increased with increase in the concentration of plant growth regulators. Similarly, the carbohydrate and protein contents in treatment with plant growth regulators were comparatively higher than those of the control. This study suggests that plants growth regulators can be utilized to enhance growth and development activities in Vernonia amygdalina Del. and that plant growth regulators such as benzyl amino purine and naphthalene acetic acid aided to maintain an appropriate shoot to root ratio and could be used to induce rooting in stem cuttings.

Determination of optimal plant growth regulators for breaking seed dormancy and micropropagation of Sorbus aucuparia L.

Sorbus aucuparia L. is an important forest tree used in the reforestation of high-altitude mountainous areas, which carries significant pharmaceutical, industrial, ornamental, food, and ecological properties. However, the seed propagation and micropropagation of mature trees of S. aucuparia L., presents various difficulties which are integral aspects for the re-propagation and breeding of novel cultivars. In this study, we isolated the mature embryo-containing seeds of selected varieties of S. aucuparia L. from their seed coat and used them directly as explants in vitro to investigate and determine the optimal dose of cytokinin in breaking seed dormancy and micropropagation. 3 mg/L of benzyl adenine (BA) in addition to different concentrations and combinations of kinetin (0.5, 1, and 2 mg/L), indole-3-butyric acid (IBA) (0, 1, 0.5, and 1 mg/L), and 1-naphthalene acetic acid (NAA) (0, 1, 0.5, and 1 mg/L) were applied within a Murashige and Skoog (1962) (MS) medium at germination inhibition, shoot elongation, and shoot proliferation. In the MS mediums containing BA and kinetin, germination was achieved at the end of 1 week, and shoot proliferation was achieved at the end of 3 weeks. The most successful germination (96%), tallest shoot length (mean 5.1 cm), most shoot proliferation (mean 7.2 pieces), and number of nodes (mean 9.7 pieces) were identified in the MS containing 3 mg/L BA and 1 mg/L kinetin. Direct root formation with shoot elongation occurred in 25% of explants which germinated in the MS medium. For shoots propagated without roots in this medium, 62% of these achieved rooting at the highest dose of 1 mg/L indole-3-acetic acid (IAA) using a two-stage rooting method. Rooted shoots were successfully transferred to an ex vitro medium. These results provide a basis for breaking seed dormancy of selected Sorbus L. genotypes quicker, leading to more effective clonal production. Keywords: Sorbus aucuparia L., rowanberry, tissue culture, seed dormancy, micropropagation, in vitro

Effect of Foliar Applications of Amino Acids, Benzyl Adenine and Nano-Fertilizers on Yield Quantity and Quality of Grapevine cv. ‘Thompson Seedless’

This study was carried out on a private vineyard, located in Sulaymaniyah governorate, Kurdistan Region-Iraq, during the growing season (2021) to investigate the effect of foliar applications of amino acids (Tecamin Max) (AA) at (0, 5, 10 mL/L), benzyl adenine (BA) at (0, 100, 200 mg/L), Nano-NPK fertilizer at (0, 2, 4 g/L) and their interactions on the productivity, physical and chemical fruit characteristics of grape cv. ‘Thompson Seedless’. The results confirmed that the highest concentrations of all applicants; AA, BA and Nano-NPK either solely or as a triple combination, recorded the higher values of the number of clusters per vine, cluster weight, total yield per vine, total sugars%, total soluble solids%, vitamin C, total carotene and total phenol contents in the berries. Triple interaction effects of AA + BA + Nano-NPK also resulted in many significant differences, in which 10 mL/L AA + 200 mg/L BA + 4 g/L Nano-NPK obtained the superior significant values of the number of clusters per vine (61.33), cluster weight (1.25 kg), total yield per vine (77.73 kg), total sugars (29.18%), and total soluble solids (29.9%). However, the highest mean value of vitamin C (3.63 mg/g f.w.) resulted from foliar application of 5 mL/L AA, both control and 100 mg/L BA and 2 g/L Nano-NPK, whereas the triple interactions of 10 mL/L AA+ 100 mg/L BA + 4 g/L Nano-NPK, and 5 mL/L AA + 0 mg/L BA + 4 g/L Nano-NPK gave the significant superior values of total carotene (3.01 mg/kg) and total phenol (1.46%) contents, respectively.

Effect of Shading Ratio, Benzyl Adenine and Organic Fertilizer ( Horti Boost 10) on the Content of Elements and some Chemical Compounds of Myrtle Plants (Myrtus communis L.)

The study was conducted in the fields of the Department of Horticulture and Garden Engineering / College of Agriculture / University of Kirkuk / Shwaro District, located at latitude (35.53°) and longitude (44.38°) for the city of Kirkuk for a period of ten months from June 2021 until April 2022, for seedlings of myrtle plant of the age The seedlings were homogeneous in size and height (2 ± 42 cm), and the study included the effect of three factors on the myrtle plant, the first factor was the percentage of shading (50% inside the wooden canopy and shading with green saran clip 75%), and the second factor was three levels of benzyl growth regulator Adenine at concentrations (0, 35, 70) mg.l-1, by two sprays, the first in October and the second in November, and the third factor was three levels of Horti boost10 organic fertilizer at concentrations (0, 1, 2) ml. Liter-1, the implementation of this study followed the design of randomized complete blocks RCBD with a factorial experiment with three replications and the number of seedlings was (2 x 3 x 3 x 3 x 2 = 108) seedlings. The results were analyzed using the SAS program and the averages were compared using the test Duncan’s polynomial at the 5% probability level, and the results showed that the effect of shading ratio at the intensity was 50% It led to a significant increase in the leaves content of nitrogen 2.76%, potassium 1.95%, protein 1.55%, respectively, and there were no significant differences between the averages when using benzyl adenine at different concentrations in the experiment. And when spraying with organic fertilizer, a significant increase was observed in most of the chemical elements, where the spraying at a concentration of 2 ml.l-1 was significantly superior to the leaf content of nitrogen, phosphorous, potassium, protein, and carbohydrate (3.18%, 0.134%, 1.93%, 1.80%, and 10.60 %) Straight.

Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques

Blood clotting has become one of the most dangerous side effects associated with Corona virus, as well as the high level of cholesterol and triglycerides in the blood. Therefore, it has become necessary to use medicinal plants that are biologically safe and containing anti-clotting compound. Feijoa sellowiana represents a prolific source diverse compounds that may have thrombolytic activity. Therefore, the main research point is the production and scaling up of a target contents that have anticoagulants by using biotechnological techniques; calli production, and bioreactors and assessed their activity through in-vivo study. Murashige and Skoog (MS) medium enriched with varying concentrations of benzyl adenine (BA) and naphthalene acetic acid (NAA) was used to cultivate calli and cell suspension cultures from F. sellowiana seeds. Bioreactors were employed to boost active constituent's production. Moreover, the bioreactor physical factors such as effect of controlled or uncontrolled pH medium were investigated. The leaves of the main plant were extracted by ethanol 70% and polar and non-polar extracts were also prepared. The ethanol extract of calli and cells resulting from bioreactors were also prepared. All prepared extracts were subjected to chemical analysis by HPLC, in-vitro antioxidant assays, in-vivo anticoagulant activity and histopathological examination. Calli and cell suspension cultures were produced by using MS medium fortified with 1 mg/L BA+ 0.1 mg/L NAA. It was found that culturing of cell cultures in a bioreactor with uncontrolled pH and aeration at the value of 0.5 L/min gave the maximum and economical fresh and dry weights of the plants. After evaluation of all extracts; it was found that the calli ethanol extract for each plant was the highest value of total phenolic and total flavonoid contents either quantitatively or qualitatively. All extracts of Feijoa had antioxidant activity. The IC50 of the DPPH of Feijoa calli extract was 13.45 μg/mL, it was also confirmed by FRAP and ABTs values. Feijoa calli extract decreased platelet aggregation by suppression of thrombin, extended aPTT, PT, bleeding and clotting times. It was safer than warfarin medication. From these findings the authors can conclude that Feijoa had highly anticoagulant activity and the calli production achieved the goal of the enhancement of the phenolic constituent and thus their activity.

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study.

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L-1), Naphthaleneacetic Acid (NAA) (1 mg L-1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L-1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L-1) and NAA (1 mg L-1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L-1) and NAA (0.25, 0.50, and 1 mg L-1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L-1 of BAP and 1 mg L-1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L-1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

In vitro propagation of Geranium wallichianum: An indigenously threatened medicinal plant in Pakistan

Geranium wallichianum is a well-known medicinal plant and has numerous ethnobotanical usages but its population in Pakistan is severely reducing due to overexploitation and over-harvesting. Moreover, the conventional propagation systems are not enough to sustain the population and meet the ever-increasing demand for this plant. Therefore, an efficient propagation system is necessary to conserve and increase the population of this plant. To control browning, citric acid, ascorbic acid and activated charcoal were added to Murashige and Skoog (MS) medium. The 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA) were used in different concentrations to induce the callus while shoot proliferation and root induction were achieved with media supplemented with 6-benzyl aminopurine (BAP), and indole-3-butyric acid (IBA), respectively. Callus was successfully induced in 5.1 days in a medium containing 15 ​μM NAA while the maximum percentage of callus induction (77.78%) was obtained from petiole segments in a medium containing 10 ​μM NAA. Benzyl aminopurine at a concentration of 15 ​μM produced 1.33 shoots per nodal segment compared with 1.0 in the control. Indole butyric acid at a concentration of 10 ​μM increased root length (2.67 ​cm). The results showed a decrease in the browning intensity and an increase in the percentage of survivals in both leaf and petiole cultures (38.88%) and (61.11%) at 0.3 ​g/L activated charcoal compared to control (0% and 5.5%), respectively. Activated charcoal also reduced the total phenolic content of the leaf segment (9.51 ​mg/g) and petiole segment (2.06 ​mg/g). The in vitro produced rooted plantlets were successfully acclimatized in small pots containing peatmoss.

Improvement of callogenesis and somatic embryogenesis by selecting optimal hormonal balance in Sarnav and Desiree potato varieties

Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of embryogenesis is widely used in improving plant materials and creating new biotechnological varieties. In our research, the optimum level of 1-naphthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) was determined in the formation of callus tissue in the local Sarnav potato variety, and the Desiree variety taken as a control. Leaf and stem explants were used in the processes of callus tissue formation and somatic embryogenesis of both varieties. We tried various plant growth regulators for callus formation and somatic embryogenesis in different ratios. The 1 mg/L NAA and 1.5 mg/L BAP were established as the best option for callus formation resulting in 92 and 100% development of callus in internodes of Desiree and Sarnav varieties, respectively. The 0.1 mg/L BAP and 0.1 mg/L gibberellic acid (GA3) resulted in almost 80% development of somatic embryogenesis in both varieties and were found as the most optimum option. These results showed that regenerated plants can be obtained from the Sarnav potato variety by somatic embryogenesis. This method can be applied to the Sarnav variety for genetic transformation studies.

Comparative Study Between the Growth Regulators Benzyl Adenine (BAP) and Thydizoronate (TDZ) and Nano Iron in the Growth and Multiplication of Citrumelo Shoots in Vitro

Research was conducted at Al-Qasim Green University in the Plant Tissue Culture Laboratory in the Department of Horticulture and Landscaping in the College of Agriculture from November 2021 to May 2022 with the aim of comparing two types of cytokinins: BAP at a concentration of 2.0 mg/L-1 and TDZ at a concentration of 0.2 mg/L-1 and two concentrations Iron (2.5-5 mg/L-1). To know their effect on the average of shoot multiplication in vitro of the root and its content of protein and macro (N.K) and Micro (iron) elements. The research was implemented as a factorial experiment using a complete random design (CRD) with two factors: cytokinin and nano iron. The results indicated a superiority of 0.2 mg.L-1 TDZ in each of the Multiplication shoots (4.667 shoots/explant ) and the content of shoots of iron (54.66 mg.kg-1), while BAP at a concentration of 2.0 mg/L-1 was excelled on the content of shoots of nitrogen, potassium and protein (1.778%, 1.947%.) respectively. As for nano iron at a concentration of 2.5 mg. L-1 was significantly excelled in the average of multiplication shoots (5 shoots/explant ), nitrogen (1.765%) and protein (10.98%). Specifically, the 5 mg dose. Potassium and iron (1.692% and 55.0 mg.kg-1) were most abundant in L-1.

Effect of Explants Type and Plant Growth Regulators on the Stimulation and Multiplication of Vegetative Branches of Trigonella foenum-graecum L. in Vitro

The study included the use of plant tissue culture technology in the use of plant parts and different combinations in the emergence and multiplication of farms of the vegetative branches of the fenugreek plant(1,2,3)mg.l-1 BA, and the second stage was carried out by cultivating the growths resulting from the nodes grown in the previous stage on the MS medium, which was prepared with different concentrations (0, 1, 2, 3) mg.l-1 BA and (0.0,0.1,0.2,0.4)mg.L-1 NAA. The study’s results showed that the concentration of 2 mg.L-1 BA achieved a response rate of 80% compared to the peaks that did not respond. The results also showed that the same concentration of benzyl adenine achieved the highest rate in the number and length of branches, and the number of leaves reached 8.69.plant-1, 4.00 cm, 26.33 leaves.plant) respectively, compared with the control treatment that achieved the lowest rate, and the concentration 0.2 mg.l-1 NAA excelled in achieving the highest rate of the number of branches and leaves reached (7.49 branches. plant-1 and 21.50 leaves.plant-1) respectively. In comparison, the concentration of 0.4 mg.l-1 NAA excelled in achieving the highest average length of branches, reaching 3.92 cm.

Organogenesis of corn plants (Zea mays L.) at various concentrations of auxin and cytokinin plant growth regulators in vitro

This study aimed to determine the effect of auxin and cytokinin concentrations as plant growth regulators (PGR) on the organogenesis of corn plants in vitro. This research was conducted from June to September 2021 in the plant tissue culture laboratory of Cokroaminoto University, Palopo City, South Sulawesi, Indonesia. The research method was a completely randomized design (CRD) consisting of 15 experimental units, i.e., P0 (control), P1 (2 ml NAA (naphthaleneacetic acid) + 1 ml BAP (benzylaminopurine)), P2 (2 ml NAA + 1.5 ml BAP), P3 (2 ml NAA + 2 ml BAP), and P4 (2 ml NAA + 2.5 ml BAP). The results revealed that the administration of auxin and cytokinin plant growth regulators had a significant effect on the parameters of germination age and plantlet weight, but it had no significant effect on the parameters of plant height, number of roots, root length, and number of leaves. The effective concentration of auxin and cytokinin growth regulators on the organogenesis of corn plants was P2 (2 ml NAA + 1.5 ml BAP) on the parameters of 11 cm plant height, 7 root strands, 1 leaf, and 1 gram plantlet weight. This was due to the influence of the given concentration of PGR, which plays multiple important roles in plant development.

Multiplication, morphogenesis and foliar abscision of Schinopsis brasiliensis Engl. in vitro

The objective was to evaluate plant growth regulators and ethylene inhibitors on the development and leaf abscission of Schinopsis brasiliensis Engl. Zeatin (ZEA) was evaluated in concentrations combined with concentrations of indolacetic acid (IAA), naphthalene acetic acid (NAA) and indolbutyric acid (IBA). ZEA and 6-benzylamino purine (BAP) were evaluated in concentrations plus a control. Ethylene inhibitors, silver nitrate and cobalt chloride were evaluated in four concentrations. The addition of 0.2 μL-1 of NAA to 0.4 μL-1 of ZEA promotes a greater number of baraúna sprouts. At concentrations of 5 and 10 μM, cobalt chloride is more efficient than silver nitrate for reducing leaf abscission in baraúna. IAA is the most suitable auxin to be associated with ZEA for higher shoot length and number of buds. Silver nitrate from a concentration of 20 μM completely avoids leaf abscission while cobalt chloride has a maximum reduction in abscission at a concentration of 40 μM.

Effect of BA on Bud Grafting in Grapefruit

Experiments were conducted at Shambat Research Station,Agricultural Research Corporation, Khartoum North, to test the effect ofconcentrations and application methods of benzyladenine (BA), as a pre-budding treatment, on scion bud-break and shoot elongation of grapefruit(Citrus paradisi Macf. cv. “Redblush”). Nine- to twelve- months old sourorange (Citrus aurantium L.) seedlings were used as rootstock. Twoapplication methods of BA (foliar and soaking) were evaluated. Theresults showed that increasing BA concentration increased all parametersmeasured relative to the controls, irrespective of the application method.The highest percentage of bud-break, the largest number of leaves, thelongest scion shoots and the most growth rating values were obtainedwith soaking in 400 mg BA/litre treatment, compared with the control orthe lower or higher BA concentrations tested or plants treated with thefoliar application method. No significant differences were obtainedamong BA concentrations tested or application methods used on scionshoot length. However, soak application method gave high values for allparameters measured compared with the soak application method.Treatment duration had no effect on the efficiency of BA on bud break.

Effect of Cultivar and Nutrient Medium on In Vitro Shoot Formation on Capitulum Explants of Gerbera (Gerbera jamesonii Bolus)

The objective of this study was to examine the shoot formationcapacity of the capitulum explants of some gerbera cultivars in differentnutrient media. Immature capitulum explants of the gerbera cultivarsAmeretto, Red Bull, Ruby Red and Yanara were cultured on Pierikmedium (P) with different concentrations of benzyladenine (BA) andRadice and Marconi medium (RM). The main P and RM media were usedeither in liquid form using cotton wool as support or solidified with agar.There was a significant interaction between the different media and thegerbera cultivars in all the parameters measured. The gerbera cultivarsshowed different responses to various media compositions with regard toshoot formation on the capitulum explant.There was a general increase invitrification rate with the increase in the BA concentration in the medium.Shoots formed in liquid media showed a high vitrification rate.

Kultur In Vitro Pisang Cavendish (Musa acuminata L.) Pada Media Dengan Konsentrasi Berbeda Ekstrak Jambu Batu Dan Benzyl Amino Purine

In vitro culture requires suitable media, the addition of BAP as a cytokinin is considered to accelerate the growth of Cavendish bananas. Constraints of browning in explants are also often found in the growth of banana explants. The addition of guava extract as an organic antioxidant and BAP is expected to suppress browning and improve the growth of Cavendish banana explants in vitro. The study was aimed to obtain the best concentration of guava extract (Psidium guajava) and Benzyl Amino Purine for the growth of Cavendish banana (Musa acuminata) explants in vitro. This study used a completely randomized design (CRD) with a factorial pattern of 4 x 3 with 4 replications. Treatment of guava extract added to the media (0 g/l, 1 g/l, 2 g/l, 3 g/l) and BAP (3 ppm, 6 ppm, 9 ppm). The data were analyzed using variance with the F test and continued with Duncan's Multiple Distance Test with 5% significance level. The results showed that the best result for the number of shoots were in the treatment of 1 g/l guava extract + BAP 3 ppm. In the number of leaves and high shoots the best result were in the treatment of 0 g/l guava extract + BAP 6 ppm