UV Filter Benzophenone-3 Research Articles

Is the commonly used UV filter benzophenone-3 a risk factor for the nervous system?

Benzophenone-3 (2-hydroxy-4-methoxybenzophenone, oxybenzone, or BP-3) is one of the most frequently used UV radiation absorbents, which are commonly referred to as sunscreen filters. Its widespread use in industrial applications provides protection against the photodegradation of a wide range of products but at the same time creates the risk of human exposure to benzophenone-3 unbeknownst to the individuals exposed. Topically applied benzophenone-3 penetrates individual skin layers, enters the bloodstream, and is excreted in the urine. In addition, benzophenone-3 easily crosses the placental barrier, which creates the risk of exposure to this substance in the prenatal period. Despite the widespread use and occurrence of benzophenone-3 in the human environment, little knowledge of the mechanisms underlying the effect of benzophenone-3 on the nervous system was available until recently. Only the most recent research, including studies by our group, has enabled the identification of new molecular mechanisms through which benzophenone-3 affects embryonic neuronal cells and the developing mammalian brain. Benzophenone-3 has been shown to induce neurotoxicity and apoptotic processes and inhibit autophagy in embryonic neuronal cells. Benzophenone-3 also alters expression and impairs function of receptors necessary for the proper development and function of the nervous system. The most worrying finding seems to be that benzophenone-3 contributes to an increased risk of developmental abnormalities and/or epigenetically based degeneration of neuronal cells by changing the epigenetic status of neuronal cells.

A green miniaturized aqueous biphasic system prepared with cholinium chloride and a phosphate salt to extract and preconcentrate personal care products in wastewater samples

A miniaturized extraction/preconcentration method based on an aqueous biphasic system (μ-ABS) was developed with reagents commonly used as food additives: cholinium chloride (ChCl) as main extraction phase, K2HPO4 as salting-out agent, and water as the main component (being the sample for analyses). With the aim of obtaining high enrichment factors, miniaturization, and adequate analytical performance, a point in the biphasic region with the lowest amount of ChCl was selected, corresponding to 1.55% (w/w) of ChCl, 59.5% (w/w) of K2HPO4, and 38.95% (w/w) of water. The green μ-ABS (attending to its main elements and performance mode) was used in combination with high-performance liquid chromatography with diode-array detection (HPLC-DAD) for the determination of 9 personal care products in wastewater samples. The μ-ABS-HPLC-DAD method showed high enrichment factors (up to 100), and quantitative extraction efficiencies for those compounds containing OH groups in their structure, which can undergo hydrogen bonding with ChCl. Thus, limits of quantification down to 0.8 µg·L-1 and extraction efficiencies between 66.4 and 108% (concentration levels of 1.3 and 13 µg·L-1) were reached for the group of parabens and the UV-filter benzophenone-3. The method is characterized by the use of non-harmful reagents and the absence of organic solvents in the entire sample preparation procedure, while being simple, low-cost, easily compatible with HPLC, and highly efficient.

Influence of suspended sediment on the bioavailability of benzophenone-3: Focus on accumulation and multi-biological effects in Daphnia magna

The UV-filter benzophenone-3 (BP3) tends to associate with suspended sediment (SPS) due to hydrophobicity, which could alter its toxicological effects on non-target aquatic organisms. In this study, the freshwater cladoceran Daphnia magna (D. magna) was selected as a model organism to investigate the impacts of the source and composition of SPS on the accumulation and multiple toxicological effects (from the molecular level to individual level) of BP3. Among the three components of SPS, amorphous organic carbon (AOC) and minerals promoted the body burden of BP3, while black carbon (BC) inhibited the bioaccumulation. The inhibition effects of BP3 on swimming and feeding behaviors of D. magna were also enhanced due to the presence of AOC and BC. Compared with BP3 exposure alone, higher oxidative stress and neurotoxicity were observed in the presence of SPS containing AOC, BC and minerals, corresponding to that superoxide dismutase, catalase and glutathione-S-transferase activities were further induced, and acetylcholinesterase activity was inhibited. Furthermore, BP3 induced mRNA expression levels of the endocrine system (ecdysone receptor, cytochrome P450 CYP314) and metabolic system (toxicant nuclear receptor HR96, P-glycoprotein), and the presence of SPS containing AOC, BC and minerals exhibited an enhanced effect. Combined with all endpoints, evident relationship was observed between the bioaccumulation level and the response of individual behavior and molecular biomarkers. The results demonstrated that the effects of SPS compositions on bioaccumulation and toxicological effects of organic UV-filters should be considered in aquatic environments.

Occurrence of Bisphenols and Benzophenone UV Filters in White-Tailed Eagles (Haliaeetus albicilla) from Smøla, Norway

There is a growing concern about the occurrence of bisphenols and benzophenone UV filters in natural ecosystems, while data are limited regarding their actual occurrence in wildlife species, especially in raptors. In this study, concentrations of bisphenol and benzophenone UV filter analogues were determined in liver tissue samples (n = 38) from white-tailed eagles (Haliaeetus albicilla) that were found dead in Smøla (2006–2018), which is a Norwegian municipality that holds one of the densest breeding populations of white-tailed eagles in Europe. Bisphenol AF (BPAF; a fluorinated analogue) was the most ubiquitous contaminant since it was detected in 32 liver samples at concentrations ranging from 1.08 to 6.68 ng/g wet weight (w.w.), followed by bisphenol A (BPA, mean 10.4 ng/g w.w.), benzophenone-1 (BzP-1, mean 3.24 ng/g w.w.), and 4-hydroxybenzophenone (4-OH-BzP, mean 0.62 ng/g w.w.). The concentrations found in livers suggested that white-tailed eagles potentially accumulate bisphenols and benzophenone UV filters, which raises concern, as these plastic and personal care product-related emerging contaminants can show endocrine-disrupting properties. The high detection frequency of the fluorinated BPAF warrants further attention as other fluorinated compounds have proven to be extremely persistent and potentially harmful to wildlife.

Distribution, removal efficiencies and environmental risk assessment of benzophenone and salicylate UV filters in WWTPs and surface waters from Romania

This article presents the environmental pollution level with organic UV filters and the effect they pose on aquatic organisms in Romania.

A solidified floating organic drop-dispersive liquid–liquid microextraction based on in situ formed fatty acid-based deep eutectic solvents for the extraction of benzophenone-UV filters from water samples

A method for simultaneous P4,4,4,12Br–DecA deep eutectic solvent formation and UV filter extraction was developed.

Efficient degradation of the organic UV filter benzophenone-3 by Sphingomonas wittichii strain BP14P isolated from WWTP sludge

Benzophenone-3 (BP3) is a widely used organic UV filter present in many environmental compartments. One way BP3 is released into the environment is through effluents from wastewater treatment plants (WWTPs). These plants are possible sources for degradation activity and WWTP sludge may potentially degrade BP3. Our goal was to identify any BP3 degrading microorganism(s) in WWTP sludge and to investigate whether the degradation was co-metabolic. Initial WWTP sludge microcosms spiked with BP3 showed 100% degradation after 20 days. Multiple transfers of these microcosms, while maintaining a strong selective pressure for BP3 degradation capabilities, resulted in the dominance of one bacterial strain. This strain was identified as Sphingomonas wittichii BP14P and was subsequently isolated. It was shown to degrade BP3 in a growth dependent manner. Strain BP14P utilized BP3 as the sole energy and carbon source and completely degraded BP3 after 7 days in minimal media. We tested the capability of BP14P to degrade nine other UV filters, but the degradation ability seemed to be restricted to BP3. However, whether this specificity is due to the lack of degradation genes, cellular transport or low bioavailability of the other UV filters remained unclear. The efficient degradation of BP3 by a group of bacteria well known for their potential for xenobiotic degradation is an important step forward for a complete risk assessment of the long-term environmental impact of BP3.

Free available chlorine initiated Baeyer-Villiger oxidation: A key mechanism for chloroform formation during aqueous chlorination of benzophenone UV filters.

Chloroform, a regulated disinfection by-product in water, is often generated during chlorination disinfection treatment. However, the formation of chloroform is heavily dependent on the molecular structures of precursors. Moreover, compounds containing ketone moiety are ubiquitous in water environments. However, it is unclear if they can generate chloroform during chlorination. In this study, 14 benzophenones (BPs), efficient and widely used UV filters, with different substituents were selected to explore chloroform formation during chlorination. All 14 BPs generated chloroform, with yields dependent on their molecular structures and operational conditions. Compounds 2,2',4,4'-tetrahydroxy-BP and benzophenone produced the highest and lowest chloroform of 0.313 and 0.013g/g, respectively, corresponding to the fastest and slowest formation rate constants of 1.41×10-1 and 2.71×10-2 min-1. Alkaline conditions and high chlorine dosages were favorable to chloroform formation. Three reactions played key roles in chloroform formation from BPs: (1) chlorine initiated Baeyer-Villiger oxidation converted ketone moieties of BP molecules into esters; (2) the esters further underwent hydrolysis and formed phenolic and benzoic products; and (3) benzoic acids underwent decarboxylation and hydrolysis to form phenolic products. Subsequently, these phenolic products could further generate chloroform in the chlorination system. More importantly, BPs could generate chloroform in the ambient water matrices during practical chlorination treatment. This work emphasized the critical role of Baeyer-Villiger oxidation for chloroform formation, implying that pollutants containing aromatic ketone moieties generate chloroform during chlorination disinfection, and their potential risk should therefore be reviewed.

Behavior of UV Filters, UV Blockers and Pharmaceuticals in High Rate Algal Ponds Treating Urban Wastewater

The present study evaluated the efficiency of a high rate algal pond (HRAP) at pilot scale to remove pharmaceuticals and personal care products (PPCPs) from urban wastewater, including UV-filters and parabens (10), benzotriazoles (4), antibiotics (15), anti-inflammatories (3) and other pharmaceuticals (3). A total of 35 compounds were targeted, of which 21 were detected in the influent wastewater to the HRAP. Removals (RE%) for pharmaceuticals were variable, with efficient eliminations for atenolol (84%) and sulfathiazole (100%), whereas the anti-inflammatories naproxen and ketoprofen were only partially removed <50%. Benzotriazoles showed elimination rates similar to those of conventional WWTPs, with RE% ranging from no elimination to 51% for the UV filter benzophenone-3 (BP3) and 100% for 4-methylbenzilidenecamphor (4MBC). Hazard quotients (HQs) were estimated for those compounds not fully eliminated in the HRAP, as well as the cumulative ecotoxicity in the resulting effluent. The majority of the compounds yielded HQs < 0.1, meaning that no environmental risk would be derived from their discharge. Overall, these results clearly indicate that HRAPs are a reliable, green and cost-effective alternative to intensive wastewater treatment, yielding promising results removing these contaminants.

Bisphenol A and benzophenone-3 exposure alters milk protein expression and its transcriptional regulation during functional differentiation of the mammary gland in vitro

The plastic monomer and plasticizer bisphenol A (BPA), and the UV-filter benzophenone-3 (BP3) have been shown to have estrogenic activities that could alter mammary gland development. Our aim was to analyze whether BPA or BP3 direct exposure affects the functional differentiation of the mammary gland using an in vitro model. Mammary organoids were obtained and isolated from 8 week-old virgin female C57BL/6 mice and were differentiated on Matrigel with medium containing lactogenic hormones and exposed to: a) vehicle (0.01% ethanol); b) 1 × 10−9 M or 1 × 10−6 M BPA; or c) 1 × 10−12 M, 1 × 10−9 M or 1 × 10−6 M BP3 for 72 h. The mRNA and protein expression of estrogen receptor alpha (ESR1) and progesterone receptor (PR) were assessed. In addition, mRNA levels of PR-B isoform, glucocorticoid receptor (GR), prolactin receptor (PRLR) and Stat5a, and protein expression of pStat5a/b were evaluated at 72 h. The mRNA and protein expression of milk proteins and their DNA methylation status were also analyzed. Although mRNA level of PRLR and GR was similar between treatments, mRNA expression of ESR1, total PR, PR-B and Stat5a was increased in organoids exposed to 1 × 10−9 M BPA and 1 × 10−12 M BP3. Total PR expression was also increased with 1 × 10−6 M BPA. Nuclear ESR1 and PR expression was observed in all treated organoids; whereas nuclear pStat5a/b alveolar cells was observed only in organoids exposed to 1 × 10−9 M BPA and 1 × 10−12 M BP3. The beta-casein mRNA level was increased in both BPA concentrations and 1 × 10−12 M BP3, which was associated with hypomethylation of its promoter. The beta-casein protein expression was only increased with 1 × 10−9 M BPA or 1 × 10−12 M BP3. In contrast, BPA exposure decreased alpha-lactalbumin mRNA expression and increased DNA methylation level in different methylation-sensitive sites of the gene. Also, 1 × 10−9 M BPA decreased alpha-lactalbumin protein expression. Our results demonstrate that BPA or BP3 exposure alters milk protein synthesis and its transcriptional regulation during mammary gland differentiation in vitro.

Consumer behavior and exposure to parabens, bisphenols, triclosan, dichlorophenols, and benzophenone-3: Results from a crowdsourced biomonitoring study.

Nearly all Americans have detectable concentrations of endocrine disrupting chemicals from consumer products in their bodies, and expert panels recommend reducing exposures. To inform exposure reduction, we investigated whether consumers who are trying to avoid certain chemicals in consumer products have lower exposures than those who are not. We also aimed to make exposure biomonitoring more widely available. We enrolled 726 participants in a crowdsourced biomonitoring study. We targeted phenolic compounds-specifically parabens, bisphenol A (BPA) and analogs bisphenol F (BPF) and bisphenol S (BPS), the UV filter benzophenone-3, the anti-microbial triclosan, 2,4-dichlorophenol, and 2,5-dichlorophenol-and collected survey data on consumer products, cleaning habits, and efforts to avoid related chemicals. We investigated associations between 68 self-reported exposure behaviors and urine concentrations of ten chemicals, and evaluated whether associations were modified by intention to avoid exposures. A large majority (87%) of participants reported taking steps to limit exposure to specific chemicals, and, overall, participants achieved lower concentrations than the general U.S. population for parabens, BPA, triclosan, and benzophenone-3 but not BPF and BPS. Participants who reported avoiding all four ingredient groups-parabens, triclosan, bisphenols, and fragrances-were twice as likely as others to be in the lowest quartile of cumulative exposure. Avoiding certain products and reading ingredient labels to avoid chemicals was most effective for parabens, triclosan, and benzophenone-3. Avoiding BPA was not effective for reducing bisphenol exposures. Avoiding certain chemicals in products was generally associated with reduced exposure for chemicals listed on labels. Greater ingredient transparency will help consumers who read labels to reduce their exposure to a wider range of potentially harmful chemicals. In order to more equitably address public health, labeling policies should be complemented by regulations that exclude harmful chemicals from consumer products.

Bioremediation of emerging micropollutants in irrigation water. The alternative of microalgae-based treatments

The present study evaluated the efficiency of a semi-closed horizontal tubular photobioreactor (PBR) at demonstrative scale to remove a total of 35 target compounds, including benzotriazoles, benzophenones, antibiotics and different pharmaceuticals present in irrigation water in a peri-urban rural area. This water run through an open channel and was a mixture of reclaimed wastewater from a nearby wastewater treatment plant (WWTP) and run-off from the different agricultural fields in the area. Most of the compounds studied are usually not fully eliminated during conventional wastewater treatment, which justifies the need to investigate alternative treatment strategies. A total of 21 of these compounds were detected in the irrigation water. Benzotriazoles were only partially removed after the microalgae treatment, with elimination rates similar to those of conventional WWTPs. The UV filter benzophenone-3 (BP3) showed variable removals, ranging from no elimination to 51%, whereas 4-methylbenzilidenecamphor (4MBC) was completely eliminated. Regarding pharmaceuticals, average removals were higher, in the range of 60–100%, with the exception of the antibiotics sulfamethoxazole (46%) and sulfapyridine, which was not removed. Despite the low biomass productivity of the PBR, parameters such as the size of the reactors, the specific mixed cultures developed and the high temperatures and pH in the closed system may account for the overall good results, The efficiency and sustainability of these systems make them a solid, feasible treatment choice.

Bioaccumulation potential of bisphenols and benzophenone UV filters: A multiresidue approach in raptor tissues

Environmental exposure to bisphenols and benzophenone UV filters has received considerable attention due to the ubiquitous occurrence of these contaminants in the environment and their potential adverse health effects. The occurrence of bisphenols and benzophenone UV filters is well established in human populations, but data is scarce for wildlife, and especially for raptors (birds of prey, falcons and owls). In this study, concentrations of eight bisphenols and five benzophenone UV filters were determined in six raptor tissues, including muscle, kidney, liver, brain, preen gland (uropygial gland) and adipose. The tissue samples (n = 44) were taken from dead raptor species (1997–2011), including Eurasian sparrowhawks (Accipiter nisus, n = 2) and long-eared owls (Asio otus, n = 2), both from France, and white-tailed eagles (Haliaeetus albicilla, n = 16) from Greenland. Overall, six bisphenols and four benzophenone UV filters were found in the samples. Bisphenol A (BPA), bisphenol F (BPF), benzophenone-8 (BzP-8) and 4-hydroxybenzophenone (4-OH-BzP) were the most abundant contaminants, accounting for median concentrations of 67.5, 3.01, 27.1 and 9.70 ng/g wet weight (w.w.), respectively. The potential role of the preen gland as a major excretory organ for bisphenols and benzophenone UV filters was suggested since the median sum concentration of the two contaminant classes in the white-tailed eagle tissues showed higher bioaccumulation potential in the preen gland (5.86 ng/g w.w.) than the liver (2.92) and kidney (0.71). The concentrations of these contaminants in the tissues of the three raptor species indicated a pattern of increasing detection rates and median concentrations with an increase of the species size and their expected trophic position. To the best of our knowledge, this is the first peer-reviewed study to document multiresidues of both contaminant classes in raptor tissues.

Dermal exposure to the UV filter benzophenone-3 during early pregnancy affects fetal growth and sex ratio of the progeny in mice.

The aim of this study was to analyze whether dermal exposure to benzophenone 3 (BP-3) during pregnancy affects critical parameters of pregnancy, and whether this exposure may affect the outcome of a second pregnancy in mice. Pregnant mice were exposed to 50-mg BP-3/kg body weight/day or olive oil (vehicle) from gestation day (gd) 0 to gd6 by dermal exposure. High-frequency ultrasound imaging was used to follow up fetal and placental growth in vivo. Blood flow parameters in uterine and umbilical arteries were analyzed by Doppler measurements. Mice were killed at gd5, gd10, and gd14 on the first pregnancy, and at gd10 and 14 on the second pregnancy. The weight of the first and second progenies was recorded, and sex ratio was analyzed. BP-3 levels were analyzed in serum and amniotic fluid. BP-3 reduced the fetal weight at gd14 and feto-placenta index of first pregnancy, with 16.13% of fetuses under the 5th percentile; arteria uterina parameters showed altered pattern at gd10. BP-3 was detected in serum 4h after the exposure at gd6, and in amniotic fluid at gd14. Offspring weight of first progeny was lower in BP-3 group. Placenta weights of BP-3 group were decreased in second pregnancy. First and second progenies of mothers exposed to BP-3 showed a higher percentage of females (female sex ratio). Dermal exposure to low dose of BP-3 during early pregnancy resulted in an intrauterine growth restriction (IUGR) phenotype, disturbed sex ratio and alterations in the growth curve of the offspring in mouse model.

Development of an analytical methodology based on fabric phase sorptive extraction followed by gas chromatography-tandem mass spectrometry to determine UV filters in environmental and recreational waters

A novel method based on fabric phase sorptive extraction (FPSE) followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) has been validated for the simultaneous determination of 11 UV filters (ethylhexyl salicylate, benzyl salicylate, homosalate, benzophenone-3, isoamylmethoxycinnamate, 4-methylbenzylidenecamphor, methyl anthranilate, etocrylene, 2-ethylhexylmethoxycinnamate, 2-ethylhexyl p-dimethylaminobenzoate, and octocrylene), in natural and recreational waters. Major experimental parameters affecting FPSE procedure have been optimized to obtain the highest extraction efficiency. Different types and sizes of sol-gel coated FPSE media, sample volume, extraction time, and type and volume of desorption solvent were evaluated. The optimal conditions involved the use of a (2.0 × 2.5) cm2 FPSE device with PDMS based coating for the extraction of 20 mL of water for 20 min. The quantitative desorption of the target compounds was performed with 0.5–1 mL of ethyl acetate. The method was satisfactorily validated in terms of linearity, precision, repeatability and reproducibility. Recovery studies were performed at different concentration levels in real water matrices to show its suitability, obtaining mean values about 90% and satisfactory precision. LODs were at the low ng L−1 in all cases. Finally, the validated FPSE-GC-MS/MS method was applied to different real samples, including environmental water (lake, river, seawater) and recreational water (swimming-pool), where 8 out of the 11 studied compounds were detected at concentrations between 0.12-123 μg L−1. FPSE is proposed as an efficient and simple alternative to other extraction and microextraction techniques for the analysis of UV filters in waters. Since no matrix effects were observed, quantification could be carried out by conventional calibration with standard solutions, without the need to perform the complete FPSE procedure, thus allowing a higher throughput in comparison with other microextraction techniques.

Modeling the fate of UV filters in subsurface: Co-metabolic degradation and the role of biomass in sorption processes

Ultraviolet filters (UVFs) are emerging organic compounds found in most water systems. They are constituents of personal care products, as well as industrial ones. The concentration of UVFs in the water bodies in space and time is mostly determined by degradation and sorption, both processes being determinant of their bioavailability and toxicity to ecosystems and humans. UVFs are a wide group of compounds, with different sorption behavior expected depending on the individual chemical properties (pKa,Koc,Kow). The goal of this work is framed in the context of improving our understanding of the sorption processes of UVFs occurring in the aquifer; that is, to evaluate the role of biomass growth, solid organic matter (SOM) and redox conditions in the characterization of sorption of a set of UVFs. We constructed a conceptual and a numerical model to evaluate the fate of selected UV filters, focused on both sorption and degradation. The models were validated with published data by Liu et al. (2013), consisting in a suite of batch experiments evaluating the fate of a cocktail of UVs under different redox conditions. The compounds evaluated included ionic UV filters (Benzophenone-3; 2-(3-t-butyl-2-hydroxy-5-methylphenyl)5-chloro-benzotriazole; 2-(2′-hydroxy-5′-octylphenyl)-benzotriazole) and neutral ones (octyl 4-methoxycinnamatte; and octocrylene).

Biomonitoring of exposure to bisphenols, benzophenones, triclosan, and triclocarban in pet dogs and cats

Similar to humans, pet animals are exposed to environmental contaminants through multiple sources and pathways. Although a few studies have demonstrated exposure of cats and dogs to environmental chemicals, little is known about exposure to bisphenols, benzophenone UV filters, and antibacterial agents. In this study, we measured three bisphenols, three benzophenone-type UV filters, triclosan (TCS), and triclocarban (TCC) in dog (n = 50) and cat urine (n = 50) collected from New York State, USA. Among bisphenols, BPS was found at the highest concentrations (mean ± SD: 3.2 ± 8.5 ng/mL in dogs and 8.85 ± 30.0 ng/mL in cats) with detection frequencies of 96% in dogs and 78% in cats. Among benzophenones, BP-3 (oxybenzone) was the dominant compound in pet urine, followed by BP-1 and BP-8. TCS was found at concentrations higher than those of TCC in both cat and dog urine. There were no significant differences in bisphenol concentrations between sexes or age groups, both in dogs and cats. The calculated hazard quotients (HQ) suggested that the current exposure levels of BPS and BP-3 in pets were 2–5 orders of magnitude below the tentative threshold values available for humans.

Acute exposure to environmentally relevant concentrations of benzophenone-3 induced genotoxicity in Poecilia reticulata

The organic UV filter benzophenone-3 (BP-3), widely used in the commercial formulations of sunscreens and personal care products, is considered an emerging pollutant and has been associated with several human and environmental health concerns. However, knowledge about their mode of action and ecotoxicity on aquatic biota is scarce. In this scenario, the objective of this work was to evaluate the genotoxic, mutagenic, and erythrotoxicity effects of BP-3 in the guppy Poecilia reticulata after acute exposure. Adult females of P. reticulata were exposed to three non-lethal and environmentally relevant concentrations of BP-3 (10, 100, and 1000 ng L−1) during 96 h of exposure, and the somatic parameter [Fulton condition factor (K)], genotoxicity (comet assay), mutagenicity [micronucleus (MN) and erythrocyte nuclear abnormalities (ENA) tests] and erythrotoxicity parameters (such as total cell area and nucleus-cytoplasmic ratio) were analyzed. Results showed that the general physiological condition (K value) of fish was not affected by acute exposure to BP-3. However, BP-3 induced DNA damage at 100 and 1000 ng L−1 and increased the frequency of total ENA at 1000 ng L−1, specially lobed nucleus, when compared to control group, indicating its genotoxic and mutagenic effects. Furthermore, the BP-3 did not induce significant changes in the total cell area and nucleus-cytoplasmic ratio. In summary, results showed that the BP-3 at environmentally relevant concentration was genotoxic to freshwater fish P. reticulata, confirming its environmental risk.

Phototoxic risk assessment on benzophenone UV filters: In vitro assessment and a theoretical model

Benzophenones (BPs), filtering out both UVA and UVB rays, are widely used in a great variety of sunscreens and personal care products. However, they have not been extensively studied for the mechanisms of UV-absorbing toxicity. In this study, we used CPZ (chlorpromazine) as a positive control and SDS (sodium dodecyl sulfate) as a negative control, and the phototoxic of BP-1, BP-3 and BP-4 were investigated in vitro assays using three cell types under different UV exposure conditions. This was followed by setting up a theoretical model, which was adopted to predict and compare the phototoxicity. It was found that Balb/c 3T3 (Balb/c 3T3 fibroblast cell lines) showed sensitivity to UVA+ and UVB+ exposure, while the HS68 (human HS68 fibroblast cell lines) to UVA+ and the HaCaT (human HaCaT keratinocyte cell lines) to UVB+. The test compound, BP-1, was detected to be phototoxic at UVA+ conditions, but BP-3 and BP-4 were discovered to be non-phototoxic at UVA+ conditions. This demonstrated that BP-1, BP-3 and BP-4 remained low-risk chemicals under UVB+ condition. The theoretical calculation of the energy gap (EGAP) showed BP-1(EGAP) > BP-3(EGAP) > BP-4(EGAP).

Degradation of UV-filter Benzophenon-3 in aqueous solution using TiO2 coated on quartz tubes.

BackgroundBenzophenone-3 (BP-3), one of the emerging pollutants, is commercially synthesized as UV filter used in cosmetics and other personal care products and its occurrence in the aquatic environment has widely been reported. The goal of this study was to enhance an AOP method for degradation of UV filter Benzophenone-3 in aqueous solutions.MethodIn this study, sol-gel method was applied to synthesis TiO2 nanoparticles. Subsequently, the nanoparticles were successfully coated on quartz tubes. The synthesized catalyst was characterized using XRD, FE-SEM and EDX analysis. Then, the efficiency of photocatalytic process using TiO2 coated quartz tubes for BP-3 degradation from synthetic and real aqueous solution was assessed.ResultThe optimum contact time and solution pH for the highest BP-3 degradation in the synthetic solution were found at 15 min and 10, respectively. The maximum degradation (98%) of BP-3 by photocatalytic process was observed at 1 mg/L initial BP-3 concentration using 225 cm2 of catalyst surface area. Among the three applied kinetic models, the experimental data were found to follow the first-order equation more closely with the rate constant of 0.2, 0.048 and 0.035 1/min for 1, 3 and 5 mg/L of initial BP-3 concentration, respectively. In order to investigate the potential of this process for real effluent, the treatment of swimming pool water and wastewater treatment plant was examined and BP-3 degradation close to 88% and 32.1 was achieved, respectively.ConclusionBased on the obtained data, the photocatalytic process could successfully be applied for water treatment in swimming pools and other effluent containing BP-3 with low turbidity. The advantage of this study is that the synthesized catalyst can be used repeatedly needless to remove catalyst from the treated solution. In addition, AOPs can effectively eliminate organic compounds in aqueous phase, rather than transferring pollutants into another phase. The limitation of this study is that in solution with high turbidity photocatalytic degradation can be hampered and pre- treatment is needed to reduce turbidity.