Benthic Research Articles

Brief communication: Testing a portable Bullard-type temperature lance confirms highly spatially heterogeneous sediment temperatures under shallow bodies of water in the Arctic

Abstract. The thermal regime in the sediment column below shallow bodies of water in Arctic permafrost controls benthic habitats and permafrost stability. We present a robust, portable device that measures detailed temperature–depth profiles of the near-surface sediments in less than 1 h. Test campaigns in the Canadian Arctic and on Svalbard have demonstrated its utility in a range of environments during winter and summer. Measured temperatures were spatially heterogeneous, even within single bodies of water. We observed the broadest temperature range in water less than 1 m deep, a zone that is not captured by single measurements in deeper water.

Baseline Inventory of Benthic Macrofauna in German Marine Protected Areas (2020-2022) before Closure for Bottom-Contact Fishing.

The response of benthic habitats and organisms to bottom-contact fishing intensity is investigated in marine protected areas (MPAs) of the German EEZ in the North and Baltic Seas. We examined the current state of macrofauna biodiversity in 2020-2022. Comparative analysis for macrofauna (in- and epifauna) inhabiting nine Natura 2000 MPAs constitutes a baseline to assess the effects of bottom-contact fishing exclusion in the future. Aspects of spatial and temporal variability are briefly summarized and discussed. We provide a species list for each region, including 481 taxa, of which 79 were found in both regions, 183 only in the North Sea, and 219 only in the Baltic Sea. The Baltic Sea dataset surprisingly included higher numbers of taxa and revealed more Red List species. The share of major taxonomic groups (polychaetes, bivalves and amphipods) in species richness showed peculiar commonalities between the two regions. In the North Sea, multivariate analysis of community structure revealed significantly higher within-similarity and stronger separation between the considered MPAs compared to the Baltic MPAs. Salinity, temperature and sediment fractions of sand were responsible for over 60% of the variation in the North Sea macrofauna occurrence data. Salinity, mud fraction and bottom-contact fishing were the most important drivers in the Baltic Sea and, together with other considered environmental drivers, were responsible for 53% of the variation. This study identifies aspects of macrofauna occurrence that may be used to assess (causes of) future changes.

Complex network of trophic interactions in Burdwood Bank, a sub-Antarctic oceanic marine protected area

The world’s oceans designated under marine protection have increased recently. Most marine protected areas (MPAs) target vulnerable, keystone, charismatic, and/or endemic species. In the sub-Antarctic, ocean protection is associated with oceanic islands, except for the MPAs Namuncurá-Burdwood Bank I and II (MPA N-BB; ~53-55°S, ~56-62°W), which are associated with a submarine plateau and a southern deep slope, respectively. We present the first analysis of the predator-prey network for the MPA N-BB, applying a topological network approach to characterise the complexity and structure of the food web and to identify the species’ role. The MPA N-BB food web consists of 1788 interactions and 379 species, with a connectance of 0.01. Almost half of the consumers feed at more than one trophic level (0.48), and the network displays a small-world pattern (short path length, high clustering of compartments). This network pattern suggests that the ecosystem might be vulnerable to perturbations targeting highly connected species, although some properties might provide resilience and resistance, resulting in a rearranged structure that preserves its original functions. Several species arise as being important in trophic structure and functioning and response to perturbations. Generalist species, mainly fishes, play a crucial role in the bentho-pelagic coupling and should be considered as relevant energy transfer agents for the ecosystem. We argue that the diversity of species, including both the benthic and pelagic habitats, is responsible for securing the connectivity within the food web to withstand perturbations, thereby contributing to the structure and stability of the ecosystem.

Numerical modelling of dispersal of Ampelisca (Amphipoda Gammaridae) during their diel migration

The aim of this study is to analyse and model the dispersal of Ampelisca, a benthic genus of amphipod crustaceans, in the fine sand community of the Bay of Morlaix (western English Channel), during their nocturnal migration in the water column. Based on sampling of the suprabenthos (in the water column adjacent to the bottom) and surface plankton on 17 June 1994, Dauvin and Zouhiri (1996) studied the vertical distribution of Ampelisca during a spring swarming reproductive period. Statistical modelling techniques and specific numerical models were combined to simulate the effects of tidal currents, wind direction and speed on Ampelisca dispersal. As an illustration to constant pluriannual seasonal pattern, the dispersion of Ampelisca individuals considered as passive particles was studied at two reproductive periods, on a short night (17th-18th June 1994) during a neap tide, and on long night (7th-8th September 1994) during a spring tide. These models are used to investigate whether Ampelisca can be dispersed beyond the fine sand community in the immediate area of their small-scale benthic habitat (only 6 km²) during their diel vertical migration. According to the results, the speed and direction of currents will determine the trajectory of Ampelisca and the degree of dispersal outside their benthic habitat during a nighttime pelagic sortie. These simulations, particularly during spring tides, are incompatible with population stability, as they appear to be excessively dispersive. Short-term dispersal simulations for twenty minutes in the plankton allow the optimal return of individuals to the fine sand benthic habitats. We hypothesize there is an adaptative advantage for Ampelisca over time to short diel migrations. However, we currently do not know whether each individual makes a single migration, or several migrations in the water column during a night.

Trophic transfer, bioaccumulation and translocation of microplastics in an international listed wetland on the Montreux record

Microplastics (MPs) are concerning emerging pollutants. Here, MPs in four edible aquatic species of different trophic levels (between ∼2 and 4), including fish species Esox lucius (Esocidae: Esocinae); Cyprinus carpio (Cyprinidae: Cyprininae); and Luciobarbus caspius (Cyprinidae: Barbinae); and the swan mussel Anodonta cygnea (Unionidae), were assessed in the Anzali freshwater ecosystem. It is a listed wetland in the Montreux record. MPs were extracted from gastrointestinal tracts (GI), gills, muscles, and skin. All the studied fish and mussels (n = 33) had MPs. MP fibres, fragments and sheets were detected in every GI examined, however, fibres were the only type of MPs in skins, muscles and gills and were the most abundant MP. The MPs found in the fish and mussels were mainly made of nylon (35% of the total MPs), polypropylene-low density polyethylene (30%), and polycarbonate (25%). The average numbers of MPs found in every fish specimen, expressed per wet body mass, had a moderate negative correlation with the condition factor (K) (MP/g - K: Pearson correlation r = −0.413, p = 0.049), and there was no significant relation with the growth factor (b) (r = −0.376; p = 0.068). Importantly, Luciobarbus caspius (with trophic level 2.7–2.8) bioaccumulated MPs and presented a strong correlation between their MP contamination and age (r = 0.916 p < 0.05). Greater gill mass (or related factors) played an important role in the accumulation of MPs, and there was a strong correlation between these factors for Esox lucius and Cyprinus carpio (r = 0.876; r = 0.846; p < 0.05 respectively). The highest MP/g gills (1.91 ± 2.65) were in the filter feeder Anodonta cygnea inhabiting the benthic zone. Esox lucius (piscivorous, trophic level 4.1) was the most contaminated species overall (a total of 83 MPs in 8 individuals, with 0.92 MP/g fish), and their gills where MPs mainly accumulated. Cyprinus carpio was the most contaminated specimen (MPs in specimens), while the number of MPs per mass unit increased with the trophic level. Their feeding and ecological behavior in the aquatic habitat affected the level of accumulation. This work includes evidence of translocation of MPs within the aquatic organisms.

Testing the applicability of the Modelling-Ongrowing Fish farms-Monitoring B (MOM-B) investigation system for assessing benthic habitat quality in the manila clam Ruditapes philippinarum aquaculture areas

The coastal aquaculture areas have been subject to a variety of anthropogenic pressures in recent studies, and reasonable environmental quality assessment is essential for both ecological conservation and production practices. However, there are significant differences between the results produced from various environmental quality assessment regarding the focus of the evaluation and the fundamental methodology. Furthermore, many of these methods are very specific and difficult to adapt to general applications. Here, we utilized the Modelling-Ongrowing fish farms-Monitoring B investigation system (MOM-B), we assessed the benthic habitat quality of benthic bivalve aquaculture areas in the Xiaoqing River estuary located in Laizhou Bay, China. The aim was to validate the accuracy and practicality of this system. The biological, chemical, and sensory parameters of the MOM-B system, temperature, chlorophyll a (Chl-a), food availability, and planktonic larvae were evaluated throughout the investigation area during the summer of 2021. The MOM-B results indicated that the benthic habitat quality in the survey area was good and lightly disturbed, but the quality in the middle tide area began to deteriorate in August, the hottest month of the summer. Environmental factors indicated that the combined effects of high temperatures and fine sedimentation had led to increased environmental stress in the middle tide area. Food availability and population recruitment also suggested that the benthic habitat quality was better in the high tide and low tide areas than in the middle tide area, and more favorable for the survival of Manila clams. The accuracy, sensitivity, and discriminatory ability of the MOM-B system were demonstrated by environmental and biological indicators. This work showed that the MOM-B system is a practical, simple, and sensitive environmental assessment tool that is easy to implement in estuarine and benthic bivalve aquaculture areas. It can be used for long-term continuous monitoring and as an early warning tool for benthic habitat quality.

Distribution of toxigenic cyanobacteria in Alpine lakes and rivers as revealed by molecular screening

The increasing frequency of cyanobacteria blooms in waterbodies caused by ecosystem eutrophication could endanger human health. This risk can be mitigated by effective monitoring incorporating molecular methods. To date, most molecular studies on toxigenic cyanobacteria have been limited to microcystins (MCs), disregarding other cyanotoxins, to freshwater planktic habitats while ignoring benthic habitats, and to limited geographic areas (usually one or a few specific waterbodies). In this study, we used PCR-based methods including PCR product sequencing and chemical-analytical methods (LC-MS/MS) to screen many plankton (n = 123) and biofilm samples (n = 113) originating from 29 Alpine lakes and 18 rivers for their cyanotoxin production potential. Both mcyE (indicating MC synthesis) and anaC (indicating anatoxin (ATX) synthesis) gene fragments were able to qualitatively predict MC or ATX occurrence. The abundance of mcyE gene fragments was significantly related to MC concentrations in plankton samples (R2 = 0.61). mcyE gene fragments indicative of MC synthesis were most abundant in planktic samples (65 %) and were assigned to the genera Planktothrix and Microcystis. However, mcyE rarely occurred in biofilms of lakes and rivers, i.e., 4 % and 5 %, respectively, and were assigned to Microcystis, Planktothrix, and Nostoc. In contrast, anaC gene fragments occurred frequently in planktic samples (14 % assigned to Tychonema, Phormidium (Microcoleus), and Oscillatoria), but also in biofilms of lakes (49 %) and rivers (18 %) and were assigned to the genera Phormidium, Oscillatoria, and Nostocales. The cyrJ gene fragment indicating cylindrospermopsin synthesis occurred only once in plankton (assigned to Dolichospermum), while saxitoxin synthesis potential was not detected. For plankton samples, monomictic and less eutrophic conditions were positively related to mcyE/MC occurrence frequency, while oligomictic conditions were related to anaC/ATX frequency. The anaC/ATX frequency in biofilm was related to the lake habitats generally showing higher biodiversity as revealed from metabarcoding in a parallel study.

Rapid morphosedimentary development of a shallow subtropical embayment in response to extreme episodic flooding

Variability in supply of terrestrial sediments to the coastal zone impacts sediment transport processes and ecological dependencies. This study investigated surface sediment distribution and transportation within a 2020 km2 shallow embayment following a period of flood induced sediment supply. Extensive field sampling of 221 common sites between 2015 and the 2019 follow-up survey presented, identified significant reduction in fine sediment fractions, evident through a 367 km2 decrease in surface mud coverage. Wind-wave sediment transport processes controlled the spatial distribution of fine sediments, exporting 23 million tonnes of mud from the shallow sedimentation zones. In a broader context, sensitive benthic habitats such as seagrass meadows exhibited signs habitat recolonisation coinciding with areas of reduced mud content. Despite these promising short-term outlooks, the developed fine sediment budget suggests ongoing terrestrial sedimentation will exhaust key sediment sinks within Moreton Bay, effectively reducing the system's ability to buffer future sedimentation events.

An experimental study of benthic habitat selection in yellow-phase American eels (Anguilla rostrata)

An experimental study of benthic habitat selection in yellow-phase American eels (Anguilla rostrata)

Deep seafloor hydrothermal vent communities buried by volcanic ash from the 2022 Hunga eruption

Mass mortality of marine animals due to volcanic ash deposition is present in the fossil record but has rarely been documented in real time. Here, using remotely-operated vehicle video footage and analysis of ash collected at the seafloor, we describe the devastating effect of the record-breaking 2022 Hunga submarine volcanic eruption on endangered and vulnerable snail and mussel species that previously thrived at nearby deep-sea hydrothermal vents. In contrast to grazing, scavenging, filter-feeding, and predatory vent taxa, we observed mass mortality, likely due to smothering during burial by thick ash deposits, of the foundation species, which rely on symbiotic chemosynthetic bacteria for the bulk of their nutrition. This is important for our broad understanding of the natural disturbance of marine ecosystems by volcanic eruptions and for predicting the effects of anthropogenic disturbance, like deep-sea mining, on these unique seafloor habitats.

Using luminescence dating to constrain lake sediment records: A new age model for the 1.38 Ma lake Malawi drill core, Eastern Africa

The 2005 Lake Malawi deep scientific drill core is the longest and most continuous high-resolution record from the continental tropics, extending to ∼1.38 Ma. While extensive sets of paleoclimate proxy data have been generated from this core, a gap in directly dated sediment, between ∼74 ka and ∼590 ka (28–167 m below lake floor), has limited the understanding of climatic drivers in this system. Previous age models fill the gap in direct dates by tuning to the global δ18O stack and interpreting paleomagnetic excursions, but these methods and the resulting models remain disputed. We fill this gap in chronology using luminescence dating, with 31 samples collected at ∼4 m resolution in this section of the core. Luminescence dating can sometimes be limited by relatively large uncertainties (typically ∼7%) and difficulty estimating the water content history of samples. We overcome these limitations by employing a high sampling density and using the sediment record to understand changes in water content during the burial period. This yields a vastly improved and robust age model that indicates changes in sedimentation rates not discernible in prior age models.

Machine learning for non-experts: A more accessible and simpler approach to automatic benthic habitat classification

Automating identification of benthic habitats from imagery, with Machine Learning (ML), is necessary to contribute efficiently and effectively to marine spatial planning. A promising method is to adapt pre-trained general convolutional neural networks (CNNs) to a new classification task (transfer learning). However, this is often inaccessible to a non-specialist, requiring large investments in computational resources and time (for user comprehension and model training). In this paper, we demonstrate a simpler transfer learning framework for classifying broad deep-sea benthic habitats. Specifically, we take an ‘off-the-shelf’ CNN (VGG16) and use it to extract features (pixel patterns) from benthic images (without further training). The default outputs of VGG16 are then fed in to a Support Vector Machine (SVM), a classical and simpler method than deep networks. For comparison, we also train the remaining classification layers of VGG16 using stochastic gradient descent. The discriminative power of these approaches is demonstrated on three benthic datasets (574–8353 images) from Norwegian waters; each using a unique imaging platform. Benthic habitats are broadly classified as Soft Substrate (sands, muds), Hard Substrate (gravels, cobbles and boulders) and Reef (Desmophyllum pertusum). We found that the relatively simplicity of the SVM classifier did not compromise performance. Results were competitive with the CNN classifier and consistently high, with test accuracy ranging from 0.87 to 0.95 (average = 0.9 (±0.04)) across datasets, somewhat increasing with dataset size. Impressively, these results were achieved 2.4–5× faster than CNN training and had significantly less dependency on high-specification hardware. Our suggested approach maximises conceptual and practical simplicity, representing a realistic baseline for novice users when approaching benthic habitat classification. This method has wide potential. It allows automated image grouping to aid annotation or further model selection, as well as screening of old-datasets. It is especially suited to offshore scenarios as it can provide quick, albeit crude, insights into habitat presence, allowing adaptation of sampling protocols in near real-time.

Implications of Heavy Metal Accumulation in Fish Feed, Water, Sediment, and Different Fish Species in a Polyculture System.

Heavy metal bioaccumulation in organisms is primarily a result of dietary uptake. The current study examines the concentrations of heavy metals (Pb, Cd, Cr, and Cu) in fish feed, water, sediment, and three fish species (Catla catla, Labeo rohita, and Cyprinus carpio) from different feeding zones in a polyculture pond system. Furthermore, associated human health risks were also evaluated. The fish samples (n = 25 for each species) were collected from 10 different fish ponds in the Kohat district, Pakistan. Heavy metals were determined using an atomic absorption spectrometer. Results revealed higher concentrations of heavy metals in sediment, followed by water. However, the concentration of heavy metals in fish feed was lower than the standard limits. In the case of fish, the bottom feeder (C. carpio) notably exhibited higher (P < 0.05) levels of heavy metals than the column feeder (L. rohita) and surface feeder (C. catla) fish. Moreover, in the liver of all fish species, the bioaccumulation of heavy metals was higher, followed by the gills. Principal component analysis (PCA) demonstrated a strong correlation of heavy metals in C. carpio gills, flesh, feed, and pond water, while the heavy metals in the liver correlated with the detected metals in sediment. The human health risk analysis shows that bottom feeder fish had higher estimated daily intake (EDI), target hazard quotient (THQ), and hazard index (HI) values (> 1). Consequently, the exposed population may experience adverse health effects. The findings of this study suggest that the bottom feeder (C. carpio) bioaccumulates a higher concentration of heavy metals than column (L. rohita) and surface feeder (C. catla) in the polyculture system.

Effects of macroalgae and sea urchin grazing pressure on zoantharians growth under laboratory conditions

In the context of ocean warming, thermophilic organisms such as zoantharians are expanding and altering shallow benthic habitats. Here, a four-month laboratory experiment was performed to examine the influence of three types of macroalgae morphotypes common in the Canary Islands (turf algae, Lobophora spp., and crustose coralline algae) on the growth of two zoantharian species, Palythoa caribaeorum and Zoanthus pulchellus. Additionally, the grazing effects of echinoids Diadema africanum and Paracentrotus lividus were assessed as facilitators of substrate colonization by means of controlling macroalgae cover. Colony and algal coverages were measured at the beginning, middle and end of the experiment, and increments were calculated. Results indicated a general decrease in zoantharian colony sizes in contact with different algal types in the absence of sea urchins. However, P. caribaeorum colonies showed significant growth in the presence of D. africanum, highlighting the ecological importance of sea urchins in zoantharian population proliferation and subsequent community modification. This study represents the first investigation into zoantharian-macroalgae interactions under controlled conditions.

Seagrass mapping across the coast of Tanzania

Seagrasses are marine flowering plants found in both temperate and tropical coastal environments, that offer vital ecosystem services and adaptations. However, due to their proximity to the shore, they are heavily impacted by human activities and climate change, endangering seagrasses and their services. To manage seagrass meadows effectively and sustainably, detailed monitoring and data on distribution and extent are essential. Unfortunately, past under-prioritization of reliable baseline data impedes understanding of ecosystem dynamics and rapid response to declines. This study employs satellite imagery, coupled with ground truthing, to comprehensively report seagrass coverage along Tanzania's mainland coast and Zanzibar islands. By utilizing machine-learning technologies, the study achieves 84.2% accuracy, and found that Tanzania has 2817 km2 of seagrass coverage, which is five times higher than previously reported. In addition, the results also provide coverage of additional benthic habitats, such as corals, rocks/rubble, and sand. This study provides the first comprehensive overview of seagrass coverage in Tanzania and will function as a valuable tool and baseline for understanding and managing these habitats. The findings suggest that seagrasses in Tanzania are still relatively abundant, but due to a number of threats, including pollution, coastal development, and climate change, these habitats require improvement in management strategies including regular monitoring to understand their status and trends in their area coverage. The study highlights the significance of remote sensing and machine learning in mapping and monitoring coastal habitats across extensive geographical areas. We aspire for the presented results to be utilized among stakeholders and researchers to develop more effective conservation measures for seagrasses in Tanzania and the Western Indian Ocean.

A high-gain circularly polarized magnetoelectric dipole antenna array with metallic radiating structure for millimeter-wave applications

A high-gain circularly polarized (CP) magnetoelectric (ME) dipole antenna with metallic radiating structure is presented. The proposed CP antenna element consists of two pairs of metallic posts with a supporting metallic plate, a metallic air-filled elliptical cavity, and a feeding slot on the bottom substrate integrated waveguide (SIW). The two pairs of modified rectangular metallic posts combined with the gaps between them act as radiating ME-dipoles which are surrounded by a metallic air-filled elliptical cavity. The above-mentioned radiating structures are fed by a modified cross-shaped coupling slot etched on the bottom feeding SIW to generate CP radiation, where loading the elliptical metallic cavity significantly improve the CP performance of the antenna element. Then, a 4 × 4 antenna array with full-corporate feeding network is designed, fabricated, and measured, which has an impedance bandwidth of 18.9 % from 37.4 to 45.2 GHz and a 3-dB axial ratio (AR) bandwidth of 14.2 % from 36 to 41.5 GHz. Thanks to the all-metal radiating structures, a maximum measured gain of 20.4 dBic has been achieved. The measured and simulated results are in good agreement.

Aging in zebrafish is associated with reduced locomotor activity and strain dependent changes in bottom dwelling and thigmotaxis.

Aging is associated with a wide range of physiological and behavioral changes in many species. Zebrafish, like humans, rodents, and birds, exhibits gradual senescence, and thus may be a useful model organism for identifying evolutionarily conserved mechanisms related to aging. Here, we compared behavior in the novel tank test of young (6-month-old) and middle aged (12-month-old) zebrafish from two strains (TL and TU) and both sexes. We find that this modest age difference results in a reduction in locomotor activity in male fish. We also found that background strain modulated the effects of age on predator avoidance behaviors related to anxiety: older female TL fish increased bottom dwelling whereas older male TU fish decreased thigmotaxis. Although there were no consistent effects of age on either short-term (within session) or long-term (next day) habituation to the novel tank, strain affected the habituation response. TL fish tended to increase their distance from the bottom of the tank whereas TU fish had no changes in bottom distance but instead tended to increase thigmotaxis. Our findings support the use of zebrafish for the study of how age affects locomotion and how genetics interacts with age and sex to alter exploratory and emotional behaviors in response to novelty.

Trophic stability and change across a sea ice cover gradient on the western Antarctic Peninsula

The western Antarctic Peninsula (AP) is experiencing significant changes to sea ice cover, altering the macroalgal cover and potentially affecting the foundation of benthic food webs. We used fatty acid signatures as dietary and physiological trophic biomarkers to test the hypothesis that a gradient of 36-88% mean annual ice cover would affect the trophic ecology of fleshy macroalgae and diverse benthic invertebrate consumers along the western AP. We used SCUBA to collect organisms from benthic rocky nearshore habitats, 5-35 m depth, at 15 study sites during April-May of 2019. There were no consistent ecosystem-scale differences in the nutritionally important polyunsaturated fatty acids or other univariate fatty acid summary categories in either the seaweeds or invertebrates across the ice gradient, but we did find site-level differences in the multivariate fatty acid signatures of all seaweeds and invertebrates. Ice cover was a significant driver of the fatty acid signatures of 5 invertebrates, including 3 sessile (an anemone, a sponge, and a tunicate) and 2 mobile consumers (a sea star and a sea urchin). The multivariate fatty acid signatures of 2 other sea stars and a limpet were not affected by the ice gradient. These results indicate that the trophic ecology and resource assimilation of sessile consumers that are more connected to the macroalgal-derived food web will be more sensitive than mobile consumers to impending changes to annual ice and macroalgal cover along the western AP.

The paleoredox context of early eukaryotic evolution: insights from the Tonian Mackenzie Mountains Supergroup, Canada.

Tonian (ca. 1000-720 Ma) marine environments are hypothesised to have experienced major redox changes coinciding with the evolution and diversification of multicellular eukaryotes. In particular, the earliest Tonian stratigraphic record features the colonisation of benthic habitats by multicellular macroscopic algae, which would have been powerful ecosystem engineers that contributed to the oxygenation of the oceans and the reorganisation of biogeochemical cycles. However, the paleoredox context of this expansion of macroalgal habitats in Tonian nearshore marine environments remains uncertain due to limited well-preserved fossils and stratigraphy. As such, the interdependent relationship between early complex life and ocean redox state is unclear. An assemblage of macrofossils including the chlorophyte macroalga Archaeochaeta guncho was recently discovered in the lower Mackenzie Mountains Supergroup in Yukon (Canada), which archives marine sedimentation from ca. 950-775 Ma, permitting investigation into environmental evolution coincident with eukaryotic ecosystem evolution and expansion. Here we present multi-proxy geochemical data from the lower Mackenzie Mountains Supergroup to constrain the paleoredox environment within which these large benthic macroalgae thrived. Two transects show evidence for basin-wide anoxic (ferruginous) oceanic conditions (i.e., high FeHR/FeT, low Fepy/FeHR), with muted redox-sensitive trace metal enrichments and possible seasonal variability. However, the weathering of sulfide minerals in the studied samples may obscure geochemical signatures of euxinic conditions. These results suggest that macroalgae colonized shallow environments in an ocean that remained dominantly anoxic with limited evidence for oxygenation until ca. 850 Ma. Collectively, these geochemical results provide novel insights into the environmental conditions surrounding the evolution and expansion of benthic macroalgae and the eventual dominance of oxygenated oceanic conditions required for the later emergence of animals.

Diet composition and variation in four commonly landed and threatened shark species in Maharashtra, India

The diets of Carcharhinus limbatus, Scoliodon laticaudus, Chiloscyllium arabicum and Ch. Griesum from the west coast of India were investigated. We compared trophic resource use and overlap across and within benthic and pelagic habitat guilds, between the two sympatric species belonging to family Hemiscyliidae, and between the life stages of S. laticaudus. It was observed that there was a significant difference in the diet composition of benthic and pelagic shark species. There was no difference in the diet of the two sympatric Chiloscyllium species. S. laticaudus showed an ontogenetic dietary niche shift from an invertebrate-rich to an ichthyophagous diet. Specifically, C. limbatus showed an ichthyophagous diet while S. laticaudus showed a diet consisting of both benthic and pelagic prey. C. arabicum, C. griesum, and S. laticaudus were less dependent on teleosts and displayed similar diet composition. The study also found that several of the prey species were commercially important fish species caught in the fisheries of Malvan. Thus a change in the relative abundance of either prey or predators due to fishing pressure could influence coastal food webs, leading to impacts both on ecosystems and fisheries.