Sorghum is one of the staple food crops in Ethiopia. However, several grainmould fungi challenge sorghum production. Graina mould fungi invasion of sorghum caryopses was studied on potato dextrose agar (PDA) and Blotter techniques of ISTA for seventy-two sorghum grain samples collected in Assosa and Metekel Zones of Benishangul Gumuz regional state, Western Ethiopia. Thirty-two fungal species were identified and grouped into nineteen genera from sorghum grain sampled in Assosa and Metekel zones during 2018 and 2019. The most frequently recovered and predominant fungal genera were Fusarium, Epicoccum, Exserohilum, Cladosporium, and Aspergillus. The results revealed that F. moniliforme, E. nigrum, E. turcicum, C. lunata, C. sphaerospermum, F. solani, A. alternata, and Cylindrocarpon spp. were the most frequent and predominate fungal species associated with sorghum grain mould in the region. The PDA was found to be the best medium for isolating mycoflora of sorghum caryopses.