Phosphate (PO43-) ions are an essential trace element in the human body and play a vital role in biological and ecological systems. However, excess consumption of PO43- ions causes various serious health issues and becomes hazardous to public safety. Thus, selective tracking and quantification of PO43- ions is a significant concern to the scientific communities. In the present article, we have introduced a chromo-fluorogenic probe APES, which instantly shows a characteristic color-fluorimetric change towards PO43- ions. APES shows an instant greenish-yellow fluorescence at 530 nm and a yellow-to-orange naked-eye visible colorimetric change in the presence of PO43- ions. The detection and quantification limit of PO43- ions employing APES is estimated as 1.5 µM and 5.0 µM respectively. The interaction affinity of APES towards PO43- ions is found by the Benesi-Hildebrand equation as 0.42 × 105 M-2 in a 20 % water-DMSO medium. Also, our modeled probe is biologically significant as it can detect and quantify PO43- ions within the range of 50% water medium. A super handy paper-strips-based detection tool also be fabricated utilizing APES which could be employed to track trace amounts of PO43- ions. The mechanism involved in the detection of PO43- ions is elucidated through 1H NMR and density functional theoretical analysis. Also to deeply entrust the selectivity with quantification capability and practical usability of our probe APES, a real sample analysis is performed to explore the fruitful real-time analysis by experimenting with different sources of water.