The objective of the article is to clarify the nature and benefits of public-private partnership and analysis of factors influencing its organization and dissemination in innovation. The usefulness of introducing public-private partnership in the innovation sphere is due to the possibility of combining state regulation of innovation development with the potential of private business, which should be considered as a carrier of managerial, human, and financial capital. Society is interested in the organization of public-private partnership in innovation because the result of achieving its strategic goal would solve several socially important problems, which include employment growth, increasing real incomes, spreading innovation, and accelerating socioeconomic progress. Methodology. During the analysis, public-private partnership was considered as a complex open system with the use of systematic analysis methodology in the study, which allowed to emphasize certain aspects of the specific nature of its operation. Results. Public-private partnership in innovation is a form of relationship that arises between the public sector and private business on a medium- or long-term basis and involves the creation of innovation infrastructure, production, and distribution of innovation products on mutually beneficial terms, which is achieved by providing a specific form financing, the common interests of the participants and the distribution of possible risks between them. The organization of public-private partnership, especially in the field of innovation, is influenced by a significant number of factors among which it is useful to outline institutional, legislative, economic, internal, and external, direct, and indirect. The institutional factor determines the nature of institutional policy that affects public-private partnerships. Legislative factors provide for legal regulation of the establishment and further operation of public-private partnership in the field of innovation. The economic factors influencing the organization of public-private partnership include credit, tax (tax holidays, reduction of income tax rates, tax credit), investment and depreciation (reduction of depreciation, increase of depreciation rates) policy. Practical implications. As a result of the analysis, several benefits that both the public sector and private business receive from the spread of public-private partnerships in innovation are outlined. The reflection of institutional, legislative, economic, and other factors of the organization of public-private partnership will allow to consider their influence for the intensification of innovative activity as a basis for increasing the competitiveness of the national economy. Value/originality. The use of systematic analysis allowed to outline the main factors that contribute or conversely the spread of public-private partnership in innovation in Ukraine. Considering their action will minimize the negative manifestations and make full use of the positive aspects, which will be an important factor in enhancing innovative growth on the basis of public-private partnership. Economic and other factors of public-private partnership will allow to take into account their impact to enhance innovation as a basis for increasing the competitiveness of the national economy.