Benchtop Testing Research Articles

Spectral Illumination System Utilizing Spherical Reflection Optics.

Fluorescence imaging microscopy has traditionally been used because of the high specificity that is achievable through fluorescence labeling techniques and optical filtering. When combined with spectral imaging technologies, fluorescence microscopy can allow for quantitative identification of multiple fluorescent labels. We are working to develop a new approach for spectral imaging that samples the fluorescence excitation spectrum and may provide increased signal strength. The enhanced signal strength may be used to provide increased spectral sensitivity and spectral, spatial, and temporal sampling capabilities. A proof of concept excitation scanning system has shown over 10-fold increase in signal to noise ratio compared to emission scanning hyperspectral imaging. Traditional hyperspectral imaging fluorescence microscopy methods often require minutes of acquisition time. We are developing a new configuration that utilizes solid state LEDs to combine multiple illumination wavelengths in a 2-mirror assembly to overcome the temporal limitations of traditional hyperspectral imaging. We have previously reported on the theoretical performance of some of the aspects of this system by using optical ray trace modeling. Here, we present results from prototyping and benchtop testing of the system, including assembly, optical characterization, and data collection. This work required the assembly and characterization of a novel excitation scanning hyperspectral microscopy system, containing 12 LEDs ranging from 365-425 nm, 12 lenses, a spherical mirror, and a flat mirror. This unique approach may reduce the long image acquisition times seen in traditional hyperspectral imaging while maintaining high specificity and sensitivity for multilabel identification and autofluorescence imaging in real time.

3D Printing Applications for Transcatheter Aortic Valve Replacement.

A combination of evolving 3D printing technologies, new 3D printable materials, and multi-disciplinary collaborations have made 3D printing applications for transcatheter aortic valve replacement (TAVR) a promising tool to promote innovation, increase procedural success, and provide a compelling educational tool. This review synthesizes the knowledge via publications and our group's experience in this area that exemplify uses of 3D printing for TAVR. Patient-specific 3D-printed models have been used for TAVR pre-procedural device sizing, benchtop prediction of procedural complications, planning for valve-in-valve and bicuspid aortic valve procedures, and more. Recent publications also demonstrate how 3D printing can be used to test assumptions about why certain complications occur during THV implantation. Finally, new materials and combinations of existing materials are starting to bridge the large divide between current 3D material and cardiac tissue properties. Several studies have demonstrated the utility of 3D printing in understanding challenges of TAVR. Innovative approaches to benchtop testing and multi-material printing have brought us closer to being able to predict how a THV will interact with a specific patient's aortic anatomy. This work to date is likely to open the door for advancements in other areas of structural heart disease, such as interventions involving the mitral valve, tricuspid valve, and left atrial appendage.

Interference and Intrusion in Wireless Sensor Networks

Wireless sensor network (WSN) systems for safety-critical, space and Internet of Things applications have recently begun to adopt open standards and commercial-off-the-shelf equipment, and persistently face challenges of malicious intrusion and spectrum coexistence. These threats are explored through Monte-Carlo simulation and benchtop testing, including matched protocol interference and sophisticated, interactive intrusion attacks. The need for expanding intrusion detection via a more holistic approach, whilst simultaneously improving WSN security, is illustrated. Discussions on WSN security, vulnerabilities, and attacks are also provided.

Oxygen Reduction Reaction Activity of Nanocolumnar Platinum Thin Films by High Pressure Sputtering

Nanocolumnar platinum thin films (Pt-TFs) were produced by high pressure sputtering (HIPS) and investigated as oxygen reduction reaction electrocatalysts for polymer electrolyte membrane fuel cells. Conventional high-density Pt-TF prepared by low pressure sputtering was also studied for comparison. Pt-TFs were deposited on a microporous layer (MPL)-like surface composed of carbon particles to mimic catalyst-coated gas diffusion electrodes. Electron microscopy imaging revealed that HIPS Pt-TFs developed a cauliflower-like columnar microstructure, which originated from a shadowing effect during HIPS. This shadowing effect is enhanced on the rough surface of the MPL-like carbon that leads to the nano-cauliflower formation. With this approach, we also aimed to relate the catalyst performance obtained by benchtop tests directly to membrane electrode assembly test results. The electrochemically active surface area of Pt-TFs increased from 10 to 19 m2 g−1 with increasing sputter pressure. Specific activity of conventional high-density and nanocolumnar films were similar at ∼600 μA cm−2, which is likely due to their similar crystal grain sizes, >5 nm. On the other hand, mass-specific (MA) activity values increased from ∼0.06 A/mgPt for conventional Pt-TF to ∼0.13 A/mgPt for HIPS Pt-TFs, which is consistent with the columnar microstructure of HIPS films providing a better catalyst utilization compared to conventional Pt-TF.

Modeling and Testing of Fluidic Flexible Matrix Composite Lead–Lag Dampers

A new lead–lag damper concept using a fluidic flexible matrix composite (F2MC) tube is presented in this paper. A model is developed for an articulated rotor blade integrated with an F2MC damper consisting of an F2MC tube, an inertia track, an orifice, and a hydraulic accumulator. Benchtop tests using a 4.5-ft rotor blade demonstrate the performance of a smallscale F2MC damper. The blade–damper system model predictions are verified by comparing experimentally measured and model-predicted frequency response data. In benchtop tests, the model predicts blade damping ratios of up to 0.34 with the F2MC damper. A simplified articulated blade based on the UH-60 rotor is simulated to assess the feasibility of a full-scale F2MC damper. Simulation results predict that the damper can generate blade damping ratios of over 0.30 at low blade lag angles.

Design and Preliminary Validation of a Lower Limb Exoskeleton With Compact and Modular Actuation

Geared motor-driven lower limb exoskeletons (LLEs) are widely researched to assist paraplegic patients with spinal cord injury in recovering locomotion ability. In order to achieve a compact exoskeleton joint design while avoiding increasing the mechanical complexity of the exoskeleton frame, this study presents a design of an LLE with compact and modular actuation. A synchronous drive and a gear drive as transmissions are used to distribute hip and knee actuators, respectively, ensuring a compact axial width. The modular design of joint actuators enables it simple to separately design or select the ergonomic exoskeleton frames. A paradigm of the LLE design is comprehensively provided, including the requirement analysis, mechanical, electrical and control system design, which can be a reference of the early development of the exoskeleton prototype. The performance of the LLE is preliminarily validated by the benchtop tests including the closed-loop speed bandwidth test and the swing test and one healthy human subject experiments including walking with the LLE disenabling all motors and walking assisted by the LLE. The results of the benchtop tests validate that the LLE has enough bandwidth of closed-loop speed, satisfactory repeatability, high precision, good antijamming capability and strong torque capacity. The results of the healthy human subject experiments validate a harmonic interaction and a good integration between the user and the ergonomic mechanical system, good performance of the electrical and control system in joints motion control of the LLE. The prototype of the LLE smoothly and successfully assists the healthy human subject in walking on the level ground. The proposed LLE is promising to be applied to assist paraplegic patients in recovering the walking ability.

A Novel, Easy-to-Use Staple Line Reinforcement for Surgical Staplers.

BackgroundStaple line reinforcement (SLR) is a popular tool used by surgeons to increase staple line strength and improve peri-operative hemostasis. However, currently marketed buttress materials require special attention in attachment to the staple anvil and cartridge and may come loose during typical maneuvering of stapling procedures. We have evaluated a new SLR that has an attachment material that affixes buttress across the entire anvil and cartridge face to prevent slipping, twisting, sliding and/or bunching.MethodsIn benchtop and preclinical testing, the new buttress material (ECHELON ENDOPATH™ Staple Line Reinforcement) was compared to a commercially available SLR for physical characteristics, including strength, absorption, security on the anvil and cartridge during stapler manipulation, impact on the tissue healing response and tissue abrasion. The two SLR’s were also compared to a staple line without buttress for hemostasis.ResultsThe new SLR was 180% stronger initially and maintained a greater strength for up to 14 days of exposure to an in vitro solution (p≤0.001), even though it was lighter and exhibited a faster rate of degradation. The new buttress material maintained complete adherence to the anvil and cartridge throughout tissue manipulation, whereas the commercial product lost substantial coverage in 72% of samples. Both SLR’s provided superior hemostasis to the non-buttress control, with minimal impact on tissue healing or abrasion.ConclusionBecause the new buttress material comes with attachment material affixed across the entire anvil and cartridge face of the stapler and maintains coverage during manipulations, it should be much easier to use. The physical characteristics of the new SLR were as good as or better than current product that requires the buttress to be applied to the cartridge and anvil. In addition, the new SLR is similar in hemostasis to standard products and superior to stapling without the use of buttress. Further research is needed to determine whether these preclinical benefits carry over into a clinical setting.

Electric Conductivity Probes to Study Change in Degree of Saturation - Bench Top Laboratory Tests

Sand characteristics such as liquefaction susceptibility can be affected as a result of change in degree of saturation of sand. New liquefaction mitigation technique by inducing partial saturation in sands is introduced by Yegian et al in 2007[1]. This technique requires to monitor changes in degree of saturation of sand. By nature, changes in degree of saturation of sand can lead in changes in its electric conductivity. Electric conductivity is the property of a material that represents its ability to conduct electric current. Fully saturated sand can conduct electric current better than sand with lower degree of saturation. Therefore, the change in measured electric conductivity can be used to calculate the change in degree of saturation of sand. In 1942, Gus Archie [2] expressed that the electric conductivity of soil is a function of its porosity, degree of saturation, tortuosity and electric conductivity of pore fluid. Using Archie’s law electrical conductivity can be related to the degree of saturation in sands. Typically, electric conductivity probes and meters are instruments which are used to measure electric conductivity. Using electrical conductivity probes, sets of bench top tests were conducted on Ottawa sand to study the relation between degree of saturation and electric conductivity in sand. Partial saturation in sands were created by pouring dry sand into sodium percarbonate solution with a known initial concentration. By nature, sodium percarbonate in water, generates oxygen gas bubbles in time. The changes in electric conductivity in the specimen were measured using electric conductivity meters and probes. In addition, changes in degree of saturation of the specimen were measured using soil phase relations equations. Measured electric conductivity data and calculated degree of saturations were correlated to explore relation between electric conductivity and degree of saturation. This paper presents results of bench top tests, and suggests a relationship between, final degree of saturation of sand and initial concentration of sodium percarbonate solution

Skin-interfaced soft microfluidic systems with modular and reusable electronics for in situ capacitive sensing of sweat loss, rate and conductivity.

Important insights into human health can be obtained through the non-invasive collection and detailed analysis of sweat, a biofluid that contains a wide range of essential biomarkers. Skin-interfaced microfluidic platforms, characterized by soft materials and thin geometries, offer a collection of capabilities for in situ capture, storage, and analysis of sweat and its constituents. In ambulatory uses cases, the ability to provide real-time feedback on sweat loss, rate and content, without visual inspection of the device, can be important. This paper introduces a low-profile skin-interfaced system that couples disposable microfluidic sampling devices with reusable 'stick-on' electrodes and wireless readout electronics that remain isolated from the sweat. An ultra-thin capping layer on the microfluidic platform permits high-sensitivity, contactless capacitive measurements of both sweat loss and sweat conductivity. This architecture avoids the potential for corrosion of the sensing components and eliminates the need for cleaning/sterilizing the electronics, thereby resulting in a cost-effective platform that is simple to use. Optimized electrode designs follow from a combination of extensive benchtop testing, analytical calculations and FEA simulations for two sensing configurations: (1) sweat rate and loss, and (2) sweat conductivity, which contains information about electrolyte content. Both configurations couple to a flexible, wireless electronics platform that digitizes and transmits information to Bluetooth-enabled devices. On-body field testing during physical exercise validates the performance of the system in scenarios of practical relevance to human health and performance.

A Proof of Concept Study of Using Machine-Learning in Artificial Aortic Valve Design: From Leaflet Design to Stress Analysis.

Artificial heart valves, used to replace diseased human heart valves, are life-saving medical devices. Currently, at the device development stage, new artificial valves are primarily assessed through time-consuming and expensive benchtop tests or animal implantation studies. Computational stress analysis using the finite element (FE) method presents an attractive alternative to physical testing. However, FE computational analysis requires a complex process of numeric modeling and simulation, as well as in-depth engineering expertise. In this proof of concept study, our objective was to develop machine learning (ML) techniques that can estimate the stress and deformation of a transcatheter aortic valve (TAV) from a given set of TAV leaflet design parameters. Two deep neural networks were developed and compared: the autoencoder-based ML-models and the direct ML-models. The ML-models were evaluated through Monte Carlo cross validation. From the results, both proposed deep neural networks could accurately estimate the deformed geometry of the TAV leaflets and the associated stress distributions within a second, with the direct ML-models (ML-model-d) having slightly larger errors. In conclusion, although this is a proof-of-concept study, the proposed ML approaches have demonstrated great potential to serve as a fast and reliable tool for future TAV design.

Pilot Program in Clinical Microbiology Laboratory Simulation for MLS Students

Medical Laboratory Science (MLS) educational programs face the challenge of placing students for clinical practica/rotations. In a non-medical university, this has been particularly true for clinical microbiology practica as local healthcare systems centralize microbiology, serology, and molecular testing services with smaller hospitals housing “stat labs” only. Encouraged by the success of a immunohematology simulation practicum implemented over 15 years ago, in the summer 2018 faculty piloted a clinical microbiology simulation lab to satisfy entry-level practical competency requirements. The pilot program consisted of three weeks of on-campus lab simulation, followed by a two-week hospital clinical experience, as opposed to five weeks in the clinical setting in the traditional practicum. In the lab simulation, students completed clinical microbiology evaluations on all clinical specimen types. Identification techniques included bench top tests, automated identification and antibiotic susceptibility testing. Students utilized rapid kit tests and performed quality control. Upon completion of the students’ practicum final exam and microbiology section of the comprehensive program exit exam, faculty evaluated student learning using two-sample t-tests to establish if statistically significant differences existed in the scores achieved by students enrolled in the pilot versus the traditional model. We hypothesized that no such differences would exist. There were no statistically significant differences between the post-practicum exam results achieved by both groups (p

Development and analysis of synoptic evaluation strategy for research platforms for Cochlear Implants and hearing aids

Cochlear Implants—Hearing Aids(CI/HA) offer opportunities for researchers wishing to advance algorithm development, since the existing commercial CIs/HAs are closed/sealed for customization, due to intellectual property and safety requirements. In general, Research Platforms (RPs), such as CCi-MOBILE (developed at CRSS-CILab, UTDallas), emulate the functionality of CIs/HAs, and allow researchers to insert their customized sub-algorithms for either benchtop (soundbooth) or field testing. To date, little formalized studies have explored RP performance effectiveness or a formal safety test paradigm. Here, a two phase comprehensive evaluation strategy is proposed that involves acoustical signal processing and user specified electrical stimulation configuration for CCi-MOBILE. While the full evaluation strategy is tedious (+407 h of audio testing: Acoustical test {380 h}, Stimulation parameter test {27 h}), an agile reduced safety test and researcher performance evaluation paradigm is developed by sub-sampling the comprehensive test and system evaluation parameter space. The synoptic safety and performance evaluation paradigm is evaluated to selectively prioritize test conditions based on: (i) acoustical diversity and electrical current level characteristics and (ii) CI stimulation configuration test conditions using physical limits of RF pulses. The proposed synoptic safety and performance evaluation paradigm is shown to be efficient, effective, and ensures reliability of each potentially deployed RP in the field.

Compliant Hybrid Gas Bearing Using Integral Hermetically Sealed Squeeze Film Dampers

AbstractThis paper focuses on an integral gas-film lubricated bearing concept developed to enable the oil-free operation of super-critical carbon dioxide (sCO2) turbomachinery. The externally pressurized tilting pad bearing concept possesses a flexible bearing support with an integral hermetically sealed squeeze film damper. Unlike the past concepts using modular hermetic squeeze film dampers presented, the bearing design in this work utilizes advanced manufacturing methods to yield an integral single piece design in efforts to reduce space envelope, cost, and improve overall design reliability. The paper advances a detailed description of the bearing design and identification of bearing support force coefficients. Nonrotating benchtop tests show the influence of vibration amplitude, frequency, and damper cavity pressurization on force coefficients for two different viscosity fluids. Results indicate an increase in stiffness and a decrease in damping when increasing the frequency of excitation. Damper cavity pressurization was shown to eliminate squeeze film cavitation for the vibration amplitudes and frequency range in the study. Additionally, the paper advances a transient fluid–structure interaction (FSI) analysis aimed at gaining insight on the interaction of flexible elements bounding a hermetic fluid volume experiencing sinusoidal vibratory motion. The analysis considers an idealized damper model with and without a vibration transmission post while varying diaphragm modulus of elasticity for three excitation frequencies. Computational results were able to capture the increase in stiffness and the decrease in damping and show that the flexibility of the bounding elements influence the damper cavity volume change and phase ultimately affecting dynamic cavity pressures and force coefficients.

Pilot study of the effect of gastrointestinal diets on fecal occult blood testing in cats.

The aim of this study was to assess the methodology of guaiac-based fecal occult blood (FOB) testing in healthy cats, and to examine the effect of feeding selected gastrointestinal diets (canned and dry) on FOB results. Stool obtained from three cats was mixed with incremental quantities of EDTA whole blood. FOB tests were performed in triplicate and interpreted at 5 mins, and at 24, 48 and 72 h post-test set-up. Ten cats were fed their normal diets, a dry hydrolyzed protein diet, a dry gastrointestinal diet and then transitioned back to their normal diet over a 7-week period. A subsequent study was repeated with similar hydrolyzed protein and gastrointestinal canned diets. Two fecal samples were tested per cat each week using human point-of-care guaiac tests. Ten microliters of whole feline blood could be reliably detected in feline stool at all time points evaluated. There was no evidence of an association between the dry hydrolyzed and dry gastrointestinal diets and a positive FOB test result (P = 0.33). Cats fed a canned gastrointestinal diet had a significantly higher probability of producing a positive FOB result than when on their normal diet (P <0.01). Feeding the canned hydrolyzed diet was not associated with an increased probability of a positive FOB test vs the normal diet (P = 0.94). Small amounts of blood in feline stool can be reliably and repeatedly detected as early as 5 mins post-FOB test set-up, making this a user-friendly bench-top screening test in a veterinary setting. Cats fed a single dry hydrolyzed, dry gastroenteric or canned hydrolyzed diet in this study had a low chance of false-positive FOB test results, while those fed the canned gastrointestinal diet had a significantly greater likelihood of producing false-positive results.

Self-Supported Nanocolumnar Platinum Thin Film Catalysts for Oxygen Reduction Reaction

Nanocolumnar platinum thin films (Pt-TFs) were produced by high pressure sputtering (HIPS) and investigated as oxygen reduction reaction electrocatalysts for polymer electrolyte membrane fuel cells. Conventional high-density Pt-TF by low pressure sputtering was also studied for comparison. Pt-TFs were deposited on a microporous layer (MPL)-like surface composed of carbon particles in order to mimic the catalyst coated gas diffusion layer in a membrane electrode assembly (MEA). Scanning electron microscopy and transmission electron microscopy images revealed that HIPS Pt-TFs developed a cauliflower-like columnar microstructures, which originated from a shadowing effect. At high working gas pressure conditions of HIPS, sputtered Pt atoms go through several gas phase collisions, lose their directionality, and can arrive at the substrate surface at oblique angles that results in the formation of columnar islands that shadow the gaps among them from incident flux of atoms. This shadowing effect is believed to be enhanced on the rough surface of MPL-like carbon particles that leads to the formation of nano-cauliflower structures. Electrochemical characterization of the samples was performed by cyclic voltammetry and rotating disk electrode measurements on Pt-TF/MPL-like-layer/glassy-carbon-insert samples in aqueous perchloric acid electrolyte. With this approach, we also aimed to relate the catalyst performance obtained by benchtop tests directly to MEA results. Electrochemically active surface area (ECSA) of PT-TFs were found to be ranging from 10 m2/g to 19 m2/g with increasing sputter pressure, which was determined from the charge for hydrogen adsorption and desorption in the cyclic voltammograms. In addition, carbon monoxide (CO)-stripping of Pt-TF coated gas diffusion electrodes were performed in a fuel cell station and similar ECSA values were recorded (ranging from 11 to 15 m2/g). This indicated that successful mimicking of a gas diffusion electrode in an MEA can be achieved by utilizing an MPL-like substrate surface for benchtop tests in aqueous electrolyte environment as opposed to directly depositing Pt-TFs on glassy carbon inserts. Specific activity (SA) values for conventional high-density and nanocolumnar films were similar that was approximately ~600 μA/cm2, which is believed to correspond to grain sizes of >5 nm as obtained from TEM and XRD analysis. On the other hand, mass-specific (MA) activity values increased from 0.06 A/mgPt for conventional Pt-TF to 0.13 A/mgPt for HIPS Pt-TFs. Higher MA agrees with the columnar microstructure of HIPS thin films that increases the surface to volume ratio of the Pt layer that provides a better catalyst utilization compared to conventional Pt-TFs.

An Elbow Exoskeleton for Upper Limb Rehabilitation With Series Elastic Actuator and Cable-Driven Differential

Movement impairments resulting from neurologic injuries, such as stroke, can be treated with robotic exoskeletons that assist with movement retraining. Exoskeleton designs benefit from low impedance and accurate torque control. We designed a 2 degree-of-freedom tethered exoskeleton that can provide independent torque control on elbow flexion/extension and forearm supination/pronation. Two identical series elastic actuators (SEAs) are used to actuate the exoskeleton. The two SEAs are coupled through a novel cable-driven differential. The exoskeleton is compact and lightweight, with a mass of 0.9 kg. Applied RMS torque errors were less than 0.19 Nm. Benchtop tests demonstrated a torque rise time of approximately 0.1 s, a torque control bandwidth of 3.7 Hz and an impedance of less than 0.03 Nm/deg at 1 Hz. The controller can simulate a stable maximum wall stiffness of 0.45 Nm/deg. The overall performance is adequate for robotic therapy applications and the novelty of the design is discussed.

Understanding How High-Protein Bar Formulations Impact Their Mechanical and Wear Behaviors Using Response Surface Analysis.

High-protein bars are popular snack items that can have significant processing issues like sticking, clogging, and cold flow. These issues are primarily problematic during formulation development because current predictive testing is reliant on highly empirical bench tests or pilot plant testing, which is expensive and time-consuming. Wear testing, which has been used in the medical field to evaluate the lifetime of soft materials used in joint replacements, may have promise in evaluating food processing ability. Wear and rheological testing were used to better understand high-protein bar processing ability. The objective of this study was to determine bench-level instrumental tests that would be able to predict processing ability for a given formulation. Two response surface designs were used for formulations of model bar systems comprising whey protein isolate (WPI), high fructose corn syrup (HFCS), and either canola oil or vegetable shortening. Ingredient formulation affected processing ability, wear behaviors, and rheological behaviors. Formulations with high ratios of WPI to HFCS and either shortening or oil exhibited good processing ability, lower wear rates, and increased elastic-type behavior, indicating that processing ability is related to formulation. The results of this study indicated that material mechanical and wear behaviors were related to processing ability; both were controlled by formulation. Because it was shown to be a good indicator of high-protein bar processing ability, wear testing of food has potential significance in benchtop testing. PRACTICAL APPLICATION: Understanding of how high-protein bar formulations impact their mechanical behaviors can help streamline their formulation development and scaling from bench to industrial production. Processing ability may be predicted with a quick wear test, providing a rapid testing method that requires little sample for evaluating bar formulations.

Methodology for Hemodynamic Assessment of a Three-Dimensional Printed Patient-Specific Vascular Test Device

Assessing hemodynamics in vasculature is important for the development of cardiovascular diagnostic parameters and evaluation of medical devices. Benchtop experiments are a safe and comprehensive preclinical method for testing new diagnostic endpoints and devices within a controlled environment. Recent advances in three-dimensional (3D) printing have enhanced benchtop tests by allowing generation of patient-specific and pathophysiologic conditions. We used 3D printing, coupled with image processing and computer-aided design (CAD), to develop a patient-specific vascular test device from clinical data. The proximal pulmonary artery (PA) tree including the main, left, and right pulmonary arteries, with a stenosis within the left PA was selected as a representative anatomy for developing the vascular test device. Three test devices representing clinically relevant stenosis severities, 90%, 80%, and 70% area stenosis, were evaluated at different cardiac outputs (COs). A mock circulatory loop (MCL) generating pathophysiologic pulmonary pressure and flow was used to evaluate the hemodynamics within the devices. The dimensionless pressure drop–velocity ratio characteristic curves for the three stenosis severities were obtained. At a fixed CO, the dimensionless pressure drop increased nonlinearly with an increase in (a) the velocity ratio for a fixed stenosis severity and (b) the stenosis severity at a specific velocity ratio. The dimensionless pressure drop observed in vivo was similar (within 1%) to that measured in moderate area stenosis of 70% because both flows were viscous dominated. The hemodynamics of the 3D printed test device can be used for evaluating diagnostic endpoints and medical devices in a preclinical setting under realistic conditions.

Robust Longitudinal Ankle Edema Assessment Using Wearable Bioimpedance Spectroscopy.

We present a robust methodology for tracking ankle edema longitudinally based on bioimpedance spectroscopy (BIS). We designed a miniaturized BIS measurement system and employed a novel calibration method that enables accurate, high-resolution measurements with substantially lower power consumption than conventional approaches. Using this state-of-the-art wearable BIS measurement system, we developed a differential measurement technique for robust assessment of ankle edema. This technique addresses many of the major challenges in longitudinal BIS-based edema assessment, including day-to-day variability in electrode placement, positional/postural variability, and intersubject variability. We first evaluated the hardware in bench-top testing, and determined the error of the bioimpedance measurements to be 0.4 Ω for the real components and 0.54 Ω for the imaginary components with a resolution of 0.2 Ω. We then validated the hardware and differential measurement technique in: 1) an ex vivo, fresh-frozen, cadaveric limb model, and 2) a cohort of 11 human subjects for proof of concept (eight healthy controls and five subjects with recently acquired acute unilateral ankle injury). The hardware design, with novel calibration methodology, and differential measurement technique can potentially enable long-term quantification of ankle edema throughout the course of rehabilitation following acute ankle injuries. This could lead to better-informed decision making regarding readiness to return to activities and/or tailoring of rehabilitation activities to an individual's changing needs.

Development of a Nanofabricated Sensor for Monitoring Intraocular Pressure via Ocular Tissue Strain.

As the number of individuals developing glaucoma is increasing, researchers and ophthalmologists are exploring new approaches to monitor intraocular pressure, which is a critical measurement for glaucoma detection. Current monitoring methods, such as implantable pressure sensors and wearable contact lenses with sensors, are being explored in eye research clinics. However, these systems currently lack in providing 24 hours data through a practical platform for large-scale use. This paper presents a novel method that provides constant measurements of the scleral strain, which is correlated with the change of intraocular pressure, using a nanofabricated discrete resistor array implant sensor. A preliminary bench-top test was performed using the sensor, and it showed that the nanofabricated 1.6 mm by 2.7 mm resistor array exhibits discrete sensing levels at increments of 41 ohms as a fixture needle traversed approximately half of the array. Though the nanosensor is in the prototype developing stage, it promises a new modality for constant, remote, and around the clock glaucoma monitoring.