Beji Village Research Articles

INFEKSI CACING Hymenolepis nana DAN Hymenolepis diminuta PADA TIKUS DAN CECURUT DI AREA PEMUKIMAN KABUPATEN BANYUMAS

The occurrence of zoonotic helminths in rats and shrews constitute serious public health risks as these animals commonly cohabit with humans, and are natural reservoirs of some helminth infections of public health importance. This study aimed to determine the prevalence of Hymenolepis diminuta and H. nana in rats and shrew and analyze the different presence of zoonotic helminth’s eggs based on species, sex and sexual maturity of rats and shrew in Banyumas District. Using cross-sectional design, this observational study was conducted on 5-14 May 2014. The trapped rats and shrews were screened for the two zoonotic helminths from the caecum of rats and shrews by the simple floatation technique. Collected data were analyzed descriptively. Results showed that out of 55 rats and shrew in Beji village 20.00 % were infected with H. diminuta and 9.09 % with H. nana, whereas out of 49 rats and shrew in Kedung Pring village 18.37 % were infected with H. nana and no H. dimunta infection were found. Infection of H. diminuta and H. nana in rats was considered to be of immense public health significance in human population who easily get infected as these animals commonly cohabit with humans

Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest