Behavior In Context Research Articles

How Likely Is it that I Would Act the Same Way: Modeling Moral Judgment During Uncertainty.

Moral rules come with exceptions, and moral judgments come with uncertainty. For instance, stealing is wrong and generally punished. Yet, it could be the case that the thief is stealing food for their family. Such information about the thief's context could flip admonishment to praise. To varying degrees, this type of uncertainty regarding the context of another person's behavior is ever-present in moral judgment. Hence, we propose a model of how people evaluate others' behavior: We argue that individuals principally judge the righteousness of another person's behavior by assessing the likelihood that they would act the same way if they were in the person's shoes. That is, if you see another person steal, you will consider the contexts where you too would steal and assess the likelihood that any of these contexts are true, given the available information. This idea can be formalized as a Bayesian model that treats moral judgment as probabilistic reasoning. We tested this model across four studies (N=601) involving either fictional moral vignettes or economic games. The studies yielded converging evidence showing that the proposed model better predicts moral judgment under uncertainty than traditional theories that emphasize social norms or perceived harm/utility. Overall, the present studies support a new model of moral judgment with the potential to unite research on social judgment, decision-making, and probabilistic reasoning. Beyond this specific model, the present studies also more generally speak to how individuals parse uncertainty by integrating across different possibilities.

Higher-Order Constructs of Injunctive and Descriptive Norm of Consumer Purchase Behaviour of Pirated Music Compact Discs

This study explores the higher-order constructs of injunctive and descriptive norm within the context of consumer purchase behavior of pirated music in Tanzania. Grounded in the Theory of Planned Behavior, the research aims to understand how subjective norm influence the decision-making processes of Tanzanian consumers regarding pirated music. A structured questionnaire was administered to a sample of 491 respondents, and data were analyzed using Smart PLS to test the relationships between the variables. These results have practical implications for marketers, business owners, and music promoters seeking to enhance CD sales among music users in Tanzania. Injunctive norm, which reflect perceived social pressures to conform to what is deemed acceptable behavior, and descriptive norm, which reflect perceptions of how others behave, were investigated as independent variables. Consumer behavior regarding the purchase of pirated music was the dependent variable. The findings reveal that both injunctive and descriptive norm have a significant impact on consumer behavior, affirming the strong influence of subjective norm on piracy-related decision- making. Based on these results, recommendations were made to Tanzanian consumers and policymakers. For consumers, the emphasis was placed on increasing awareness of the ethical and legal implications of purchasing pirated music. For policymakers, strategies were suggested to strengthen anti-piracy campaigns and to promote positive behavioral changes through normative interventions. These insights provide a valuable contribution to the understanding of consumer behavior in emerging markets, where social norms play a critical role in shaping illegal consumption patterns.

The ecology of opportunity: connecting socioecological health and school achievement in Alabama

ABSTRACT This study investigates the association between school-level achievement and the relative health of the social environment in which children are embedded, to gain a more comprehensive understanding of how behavioural, social, physical, and socio-emotional contexts may holistically impact learning. While research has recognised how specific aspects of a child’s environment can impact learning, what is less understood is how multiple facets of socioecological conditions may holistically contribute to learning advantages or disadvantages. To address this, we combine seven years and over 9,000 observations of school-level achievement scores from the U.S. state of Alabama with a comprehensive scale of “socioecological health” – a measure that includes multiple indicators such as nutrition access, housing quality, neighbourhood violence, social supports, income inequality, and mental health access. We then employ a three-level hierarchical linear modelling approach to assess the association between socioecological health and school achievement. Results suggest that (1) higher levels of socioecological health are associated with higher levels of school achievement; (2) effects are strongest for older students and rural students; and (3) healthy behaviours, social supports, and positive physical environment correspond with higher school achievement. These results highlight the breadth to which social and environmental determinants may influence learning opportunities and underscores the need for a more holistic and inclusive approach to educational leadership and social policy.

Consumer skepticism towards organic beauty products Cross-country research

Based on the attitude–behaviour–context (ABC) theory, the present study addressed consumer skepticism as one of the inhibitors of organic purchasing behaviour. More specifically, it investigated the role of environmental concern and environmental knowledge as a mediator, in the organic cosmetics background. Data gathered from consumers in Tunisia (N = 736), Italy (N = 720) and France (N = 715) and analysed using a structural equation modelling approach. The findings revealed that green skepticism is a strong inhibitor towards adoption of organic cosmetic products among consumers in the three countries. On the other hand, findings revealed that environmental knowledge and environmental concern fully mediate the relationship between green skepticism and organic purchasing behaviour. The ultimate goal is to provide valuable insights for business leaders, policymakers and marketers fully understand consumers’ resistance to organic cosmetic products. Further, it offers a comprehensive framework to support strategies to reduce consumers’ skepticism towards organic products, in different market segments.

The psychosocial background of dental care avoidance: A qualitative study.

Dental care avoidance affects individuals' oral health status. There is limited literature examining the context of dental avoidance behavior. The aim of the study is to examine attitudes toward dental care services among adults. Overall, 16 patients participated in semi-structured interviews. The interviews were analyzed using inductive thematic analysis. Through independent reviews of the themes that comprise the patients' experience, a consensus was established among the researchers. Ten major themes were identified that encapsulate patients' attitudes toward dental care attendance: Effect of environment and equipment, Fear and anesthesia, Procrastination, Dissatisfaction with the dental care system, Expectations of dentists, Quality of dental care, Edentulousness and social status, Financial involvement, Aesthetics, and Dentists' behavior. Compliance with oral health maintenance can be enhanced by specific training for professionals and dentistry students and by fine tuning the dental care system. This is the first comprehensive study in Hungary investigating attitudes among dentist patients toward dental care. The results suggest that there are many factors that may affect dental care attendance. Thus, these findings make a potentially fundamental contribution to the improvement of dental care and support practitioners in enriching their understanding of dental avoidance behavior.

8302 A Novel Tool to Map Estrogen Responsiveness Within Estrogen Sensitive Hypothalamic Neurons in Mice

Abstract Disclosure: A.L. Cara: None. A.L. Hall: None. K.K. Soma: None. J. van Veen: None. S.M. Correa: None. Identification of hormone sensitive cells has been traditionally evaluated by hormone receptor expression. Receptor positive cells can be labeled via expression of mRNA transcript via in situ hybridization, presence of protein via immunohistology, or via conditionally-dependent Cre recombinase driven reporter expression. However, these methods used to label and manipulate cells only indicate which cells are capable of sensing hormones, and not necessarily which cells are responding to a hormonal signal in a specific physiologic or behavioral context. Furthermore, broad labeling of all receptor positive cells may not accurately reflect the heterogeneous nature of certain cell populations, particularly in the brain. The hypothalamus is a brain region with well characterized hormone receptor expression, including estrogen receptors, such as ERα (encoded by the Esr1 gene). Estrogens have a broad array of physiologic and behavioral functions which are mediated through hypothalamic nuclei, including thermoregulation, metabolism, and reproduction. Neuronal populations mediating these functions have been characterized, targeted, and manipulated based on ERα expression, but the dynamic response to estrogens in these highly heterogeneous populations has yet to be uncovered. We developed a custom reporter virus that fluorescently labels estrogen-sensitive and responsive cells. The virus contains a flip-excision switch (FLEX) to drive Cre-dependent expression of a FusionRed fluorescent reporter, eight tandem estrogen response elements (ERE), and a destabilized enhanced green fluorescent protein (EGFP) with a 2-hour half-life. The Cre-dependent expression of FusionRed identifies all Esr1-Cre cells at the time of transduction, while the ERE-EGFP identifies a subset of Esr1-Cre+ cells that are actively responding to estrogens via nuclear receptor signaling. We stereotactically injected the ERE-EGFP virus to distinct hypothalamic nuclei of Esr1-Cre female mice. Following transduction, mice were sacrificed during different stages of the estrous cycle. Spatial analysis of ERE-EGFP expression revealed dynamic changes across the estrous cycle, with responses differing based on hypothalamic nucleus. Reporter expression dynamics were compared to estrogen levels measured by LC-MS/MS in ovary, serum, and microdissected brain tissue. A separate group of mice were ovariectomized and given estradiol benzoate (EB) or vehicle control, and neurons were flow sorted for bulk RNA sequencing. We discovered transcriptomic differences in FusionRed+ cells with high or low ERE-EGFP expression, including upregulation of estrogen target genes (e.g., Pgr, encoding progesterone receptor) with EB treatment in ERE-EGFP+ cells. In summary, this new viral reporter reveals divergent responses to estrogens across subsets of estrogen sensitive neurons, brain regions, and hormonal states. Presentation: 6/2/2024

Dance displays in gibbons: biological and linguistic perspectives on structured, intentional, and rhythmic body movement.

Female crested gibbons (genus Nomascus)perform conspicuous sequences of twitching movements involving the rump and extremities. However, these dances have attracted little scientific attention and their structure and meaning remain largely obscure. Here we analyse close-range video recordings of captive crested gibbons, extracting descriptions of dance in four species (N. annamensis, N. gabriellae, N. leucogenys and N. siki). In addition, we report results from a survey amongst relevant professionals clarifying behavioural contexts of dance in captive and wild crested gibbons. Our results demonstrate that dances in Nomascus represent a common and intentional form of visual communication restricted to sexually mature females. Whilst primarily used as a proceptive signal to solicit copulation, dances occur in a wide range of contexts related to arousal and/or frustration in captivity. A linguistically informed view of this sequential behaviour demonstrates that movement withindances is organizedin groups andfollows an isochronous rhythm-patterns not described for visual displays in other non-human primates. We argue that applying the concept of dance to gibbons allows us to expand our understanding of communication in non-human primates and todevelop hypotheses on the rules and regularities characterising it. We propose that crestedgibbon dances likely evolved from less elaborate rhythmic proceptive signals, similar to those found in siamangs. Although dance displays in humans and crested gibbons share a number of key characteristics, they cannot be assumed to be homologous. Nevertheless, gibbon dances represent a strikingmodel behaviour to investigate the use of complex gestural signals in hominoid primates.

Neural circuit mechanisms underlying context-specific halting in Drosophila

Walking is a complex motor programme involving coordinated and distributed activity across the brain and the spinal cord. Halting appropriately at the correct time is a critical component of walking control. Despite progress in identifying neurons driving halting1–6, the underlying neural circuit mechanisms responsible for overruling the competing walking state remain unclear. Here, using connectome-informed models7–9 and functional studies, we explain two fundamental mechanisms by which Drosophila implement context-appropriate halting. The first mechanism (‘walk-OFF’) relies on GABAergic neurons that inhibit specific descending walking commands in the brain, whereas the second mechanism (‘brake’) relies on excitatory cholinergic neurons in the nerve cord that lead to an active arrest of stepping movements. We show that two neurons that deploy the walk-OFF mechanism inhibit distinct populations of walking-promotion neurons, leading to differential halting of forward walking or turning. The brake neurons, by constrast, override all walking commands by simultaneously inhibiting descending walking-promotion neurons and increasing the resistance at the leg joints. We characterized two behavioural contexts in which the distinct halting mechanisms were used by the animal in a mutually exclusive manner: the walk-OFF mechanism was engaged for halting during feeding and the brake mechanism was engaged for halting and stability during grooming.

Discrimination between the facial gestures of vocalising and non-vocalising lemurs and small apes using deep learning

Facial expression studies in animal communication are essential. However, manual inspection methods are only practical for small datasets. Deep learning techniques can help discriminate facial configurations associated with vocalisations over large datasets. We extracted and labelled frames of different primate species, trained deep-learning models to identify key points on their faces, and computed distances between them to identify facial gestures. We used machine learning algorithms to classify vocalised and non-vocalised gestures across different species. The algorithms showed higher-than-chance correct classification rates, with some exceeding 90 %. Our work employs deep learning to map primate facial gestures and offers an innovative application of pose estimation systems. Our approach facilitates the investigation of facial repertoire across primate species and behavioural contexts, enabling comparative research in primate communication.

Prehatch Calls and Coordinated Birth in Turtles.

Hatching synchronisation is widespread in oviparous taxa. It has been demonstrated that many species use sounds to coordinate synchronous hatching, being widespread among archosaurs (birds and crocodilians). Recent studies have shown that some turtle species produce vocalisations from within the egg, but the role of this behaviour in synchronising hatch is untested. The small amount of information about sound production by turtle embryos, limited to a handful of closely related species, precludes any inferences based on differences in their ecology, reproductive behaviour and phylogenetic context. With the goal to investigate if coordinated synchronous behaviour is mediated by within-egg vocalisations in turtles, we recorded clutches from six different turtle species. The selected animals present different ecological and reproductive niches and belong to distinct phylogenetic lineages at the family level. We aimed to understand: (1) what is the phylogenetic distribution of within-egg vocal behaviour among turtles; (2) if asynchronous turtle species vocalise from within the egg; (3) if clutch size influences synchronous behaviour and (4) if within-egg turtle calls follow any phylogenetic signal. The new evidence provides light to the current knowledge about synchronous behaviour and within-egg calls, challenging previous hypothesis that within-egg sounds are accidentally produced as side-effects of other behaviours.

Traits Underlying Experimentally Evolved Dispersal Behavior in Tribolium castaneum

AbstractDispersal is an important behavior in many animals, with profound effects on individual fitness and the evolutionary trajectories of populations. This is especially true within taxa with particular life-history strategies, for example those that exploit ephemeral habitat. Further, dispersal is commonly seen to be part of behavioral syndromes - suites of traits that covary across behavioral contexts. The red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae), is a major post-harvest crop pest responsible for large losses through the infestation of stored grain. In this system dispersal is known to have a strong genetic basis and differential artificial selection on dispersal traits produces strong phenotypic divergence. However, it is unknown which traits are able to rapidly evolve to produce these results, or which behavioral components underlie differences in dispersal. Using replicate lines of T. castaneum previously selected for divergent dispersal behavior, we test for correlated activity and movement patterns, morphology and substrate surface use. We find robustly repeatable associations between the dispersal phenotype and higher activity, straighter paths, larger body size (but not relative leg length) and increased tendency to remain at the surface of fodder. Together our results suggest that dispersal is part of a syndrome of traits in T. castaneum, and must be treated as such when considering the evolution of dispersal in this system, and in attempting to predict and control its spread.

Aggressive Behavior in Older Preschoolers: Implications, Causes, and Prevention Strategies

Aggressive behavior in older preschoolers has long been a nuisance to kindergarten teachers and parents, and it is a common yet difficult problem in society. This study recorded the proportion, context, and reasons for aggressive behavior in older preschoolers at the Tianfu New Area Experimental Kindergarten in Chengdu. Combining the physical and mental development patterns of older preschoolers, methods such as observation records and situational analysis were used. Through real confrontation, observation, analysis, and exploration, effective ways to reduce and correct aggressive behavior in older preschoolers were found, with the aim of helping teachers cope with such behaviors.

Spatial maps in piriform cortex during olfactory navigation.

Odors are a fundamental part of the sensory environment used by animals to inform behaviors such as foraging and navigation1,2. Primary olfactory (piriform) cortex is thought to be dedicated to encoding odor identity3-8. Here, using neural ensemble recordings in freely moving rats performing a novel odor-cued spatial choice task, we show that posterior piriform cortex neurons also carry a robust spatial map of the environment. Piriform spatial maps were stable across behavioral contexts independent of olfactory drive or reward availability, and the accuracy of spatial information carried by individual neurons depended on the strength of their functional coupling to the hippocampal theta rhythm. Ensembles of piriform neurons concurrently represented odor identity as well as spatial locations of animals, forming an "olfactory-place map". Our results reveal a previously unknown function for piriform cortex in spatial cognition and suggest that it is well-suited to form odor-place associations and guide olfactory cued spatial navigation.

Finite Element Modeling of Beam-to-Column Steel Timber Composite Joints with Different Parameters

This study presents a comprehensive three-dimensional finite element modeling and parametric analysis of composite beam-to-column joints in steel–timber composite structures. The investigation encompassed a variety of shear connector configurations, end plate designs, and bolt dimensions, aiming to elucidate their respective influences on the structural performance and behavior of these joints. Through meticulous numerical simulation, this research sought to enhance the understanding of the interactions and load transfer mechanisms within composite connections, thereby contributing to the optimization of design practices in the field of structural engineering. The load–displacement relationship for timber–steel composite joints subjected to monotonic loading was investigated using ABAQUS 6.14 software. This study systematically analyzed the effects of various parameters, including different configurations of shear connectors, end plate thicknesses, and bolt dimensions, on the overall performance of the joints. Through this comprehensive numerical analysis, the research aimed to provide deeper insights into the mechanical behavior and structural integrity of these composite connections under the applied loading conditions. A non-linear finite element model of timber was developed and verified with the results of the experiment in this study. The findings are discussed in detail, highlighting the intricate relationships between the selected parameters and their respective effects on the performance and overall stability of the composite connections. This thorough evaluation aimed to enhance the understanding of how these variables interact within the context of composite joint design and behavior. Finally, design recommendations for composite structures, such as the dimensions of the bolt, end plate thickness, and different sizes of shear connectors are provided.

Kinematically distinct saccades are used in a context-dependent manner by larval zebrafish

Saccades are rapid eye movements that are used by all species with good vision. In this study, we set out to characterize the complete repertoire of larval zebrafish horizontal saccades to gain insight into their contributions to visually guided behavior and underlying neural control. We identified five saccade types, defined by systematic differences in kinematics and binocular coordination, which were differentially expressed across a variety of behavioral contexts. Conjugate saccades formed a large group that serves at least four functions. These include fast phases of the optokinetic nystagmus, visual scanning in stationary animals, and shifting gaze in coordination with body turns. In addition, we discovered a previously undescribed pattern of eye-body coordination in which small conjugate saccades partially oppose head rotation to maintain gaze during forward locomotion. Convergent saccades were coordinated with body movements to foveate prey targets during hunting. Detailed kinematic analysis showed that conjugate and convergent saccades differed in the millisecond coordination of the eyes and body and followed distinct velocity main sequence relationships. This challenges the prevailing view that all horizontal saccades are controlled by a common brainstem circuit and instead indicates saccade-type-specific neural control.

The relationship between morningness-eveningness and naturalness bias

ABSTRACT The naturalness bias in which people perceive natural items to be safer, healthier, and better than synthetic alternatives has been found to be associated with numerous individual difference variables (e.g. connectedness to nature and religiosity). However, no research has examined the role of morningness-eveningness in influencing preferences for naturalness. Here, we propose that evening individuals may exhibit a weaker preference for naturalness compared to morning individuals due to their greater exposure to artificial lighting, technology, and stimuli. To systematically test our theoretical perspective, we conducted three complementary and high-powered studies. In an online survey (Study 1), student participants with a stronger evening orientation displayed a diminished preference for natural drugs compared to those with a morning orientation. Using a sample of community adults, Study 2 replicated the findings of Study 1 in a real-world, behavioral context. Study 3 examined the relationship between morningness-eveningness and preference for naturalness within the domain of beverages. The results revealed that individuals with an evening-orientation had decreased odds of selecting natural water without minerals. Taken together, the findings suggest that an individual’s diurnal preference toward eveningness may have implications for their bias toward and perception of naturalness across various domains.

Vocalisation in wild-living mountain gazelles (Gazella gazella): structure and context of acoustical signals

Abstract Describing vocalisations of species in the wild is an important step to understanding their function. A wild-living population of mountain gazelles (Gazella gazella) was monitored in Israel, using camera traps, thereby providing a first detailed description of the acoustic repertoire. We described six acoustical signals that were either reported by previous authors or that were hitherto not reported. Acoustic signals were categorised according to behavioural context as alarm calls, courtship calls or threat calls and — if possible — characterised by four acoustic variables, i.e., duration, maximum amplitude frequency, three power quartiles and fundamental frequency. Vocalisations were illustrated as spectrograms supplemented by full video sequences to show the acoustical signal in its environmental, social, and behavioural context. Given the rather inconspicuous character of many acoustic signals, we proposed further in-depth studies of vocalisation in mountain gazelles and other Antilopine species to unravel new insights into their behaviour and social organisation.

Examining the Attitude–Behavior Gap in EU Ecolabel Adoption: A Mediation Path Analysis

The EU Ecolabel, established in 1992, aims to promote sustainable consumption, yet a significant gap persists between consumers’ positive environmental attitudes and their actual purchasing behavior of ecolabeled products. This study examines the attitude–behavior gap in EU Ecolabel adoption across Europe, leveraging data from the 2023 Eurobarometer survey with 26,630 valid responses from all EU member states. Using mediation path analysis, the research investigates the factors mediating the relationship between environmental knowledge and purchasing behavior, including environmental attitude, ecolabel trust, and environmental concern. The study applies an integrated theoretical framework combining the Knowledge–Attitude–Behavior model and Attitude–Behavior–Context theory to explain the complexities of consumer behavior towards ecolabeled products. The findings reveal the interplay of direct and indirect effects among ecolabel knowledge, trust, environmental concern, attitude, and buying behavior, while also considering demographic factors’ influence such as age, gender, education level, and residential setting. The research provides a comprehensive cross-country analysis within the EU, offering insights into bridging the attitude–behavior gap and enhancing the adoption of eco-friendly products. The results have significant implications for policymakers, marketers, and researchers in promoting sustainable consumer behavior, improving environmental label effectiveness, and developing targeted interventions to increase ecolabel adoption rates.

Learning to use landmarks for navigation amplifies their representation in retrosplenial cortex.

Visual landmarks provide powerful reference signals for efficient navigation by altering the activity of spatially tuned neurons, such as place cells, head direction cells, and grid cells. To understand the neural mechanism by which landmarks exert such strong influence, it is necessary to identify how these visual features gain spatial meaning. In this study, we characterized visual landmark representations in mouse retrosplenial cortex (RSC) using chronic two-photon imaging of the same neuronal ensembles over the course of spatial learning. We found a pronounced increase in landmark-referenced activity in RSC neurons that, once established, remained stable across days. Changing behavioral context by uncoupling treadmill motion from visual feedback systematically altered neuronal responses associated with the coherence between visual scene flow speed and self-motion. To explore potential underlying mechanisms, we modeled how burst firing, mediated by supralinear somatodendritic interactions, could efficiently mediate context- and coherence-dependent integration of landmark information. Our results show that visual encoding shifts to landmark-referenced and context-dependent codes as these cues take on spatial meaning during learning.

The Rpfor gene modulates the locomotory activity and host-seeking behaviour of Rhodnius prolixus.

The molecular bases of animal behaviour are intricate due to the pleiotropic nature of behaviour-modulating genes, which are often expressed across multiple tissues. The foraging gene (for) encodes a cGMP-dependent protein kinase (PKG), pivotal in regulating downstream target proteins through phosphorylation. In insects, for has been implicated in various behavioural contexts and physiological processes regarding searching for food. Rhodnius prolixus, a hematophagous bug that transmits Trypanosoma cruzi, the causative agent of Chagas disease, exhibits specific activity patterns associated with its hematophagous behaviour. Our previous work demonstrated a correlation between locomotor activity profiles and the expression of Rpfor, suggesting its involvement in modulating triatomine locomotion. In this study, we investigated the impact of Rpfor knockdown on locomotory activity, host-seeking behaviour, feeding performance and lipid metabolism in R. prolixus nymphs. Using RNA interference, we achieved a significant reduction of Rpfor expression in both the brain and fat body of R. prolixus nymphs. Knocked-down nymphs exhibited diminished non-oriented locomotory activity compared with controls, without altering the characteristic bimodal pattern of activity. Additionally, they displayed an increased tendency to approach a host, suggesting a role for Rpfor in modulating host-seeking behaviour. Feeding performance and lipid metabolism remained unaffected by Rpfor knockdown. Our findings underscore the multifaceted role of Rpfor in modulating locomotor activity and host-seeking behaviour in R. prolixus nymphs, shedding light on the molecular mechanisms underlying their hematophagous behaviour and potential implications for disease transmission. Further research is necessary to elucidate the intricate interplay between Rpfor expression, behaviour and physiological processes in triatomine bugs.