Growth Of Bean Research Articles

Intercropping improves faba bean photosynthesis and reduces disease caused by Fusarium commune and cinnamic acid-induced stress

Modern intensive cropping systems often contribute to the accumulation of phenolic acids in the soil, which promotes the development of soilborne diseases. This can be suppressed by intercropping. This study analyzed the effects of intercropping on Fusarium wilt based on its effect on photosynthesis under stress by the combination of Fusarium commune and cinnamic acid. The control was not inoculated with F. commune, while the faba bean plants (Vicia faba L.) were inoculated with this pathogen in the other treatments. The infected plants were also treated with cinnamic acid. This study examined the development of Fusarium wilt together with its effects on the leaves, absorption of nutrients, chlorophyll fluorescence parameters, contents of photosynthetic pigments, activities of photosynthetic enzymes, gas exchange parameters, and the photosynthetic assimilates of faba bean from monocropping and intercropping systems. Under monocropping conditions, the leaves of the plants inoculated with F. commune grew significantly less, and there was enhanced occurrence of the Fusarium wilt compared with the control. Compared with the plants solely inoculated with F. commune, the exogenous addition of cinnamic acid to the infected plants significantly further reduced the growth of faba bean leaves and increased the occurrence of Fusarium wilt. A comparison of the combination of F. commune and cinnamic acid in intercropped wheat and faba bean compared with monocropping showed that intercropping improved the absorption of nutrients, increased photosynthetic pigments and its contents, electron transport, photosynthetic enzymes, and photosynthetic assimilates. The combination of these factors reduced the occurrence of Fusarium wilt in faba bean and increased the growth of its leaves. These results showed that intercropping improved the photosynthesis, which promoted the growth of faba bean, thus, reducing the development of Fusarium wilt following the stress of infection by F. commune and cinnamic acid. This research should provide more information to enhance sustainable agriculture.

The rhizosphere of the pioneer plant Dicranopteris dichotoma (Thunb.) Bernh. contains powerful and robust rhizosphere growth-promoting bacteria

Rhizosphere microorganisms are important for maintaining soil and plant health and promoting plant growth and development. Screening rhizosphere growth-promoting bacteria with unique habitats, high activity and multiple functions is conducive to exploring the potential stress resistance of growth-promoting bacteria, and further improving the applicability of microbial fertilizers in agricultural production in response to the development concept of green agriculture. The aim of this study was to investigate the physiological activity of the culturable core growth-promoting bacteria of Dicranopteris dichotoma and verify their growth-promoting effect on mung bean (Vigna radiata) growth. A total of 41 bacterial strains were isolated in this study; 21 strains had more than one physiological activity, among which the highest siderophores units (SU) were 97.1%, and the highest IAA yield was 134.46 mg/L. According to morphology and 16S rRNA, three strains of Bacillus sp. and one strain of Pantoea sp. were screened, all of which were predicted to have the functions of phosphorous solubilization, cellulase production, IAA production and siderophores production. In a pot experiment, these four strains were inoculated in the rhizosphere soil every 7 days for a total of 4 times, and the aerial and underground biomass, plant height, root length and leaf number of V. radiata plants increased significantly 30 days after the last inoculation. The strains isolated in this study have excellent plant growth promoting (PGP) properties and were expected to be used as a new biological inoculant in practical production.

Field evaluation of biostimulants on growth, flowering, yield, and quality of snap beans in subtropical environment

The cultivation of snap beans (Phaseolus vulgaris L.) in subtropical regions faces environmental challenges leading to potential declines in yield. This study explores the efficacy of biostimulants as a solution, specifically investigating spraying treatments with 6-benzylaminopurine (6-BA), chitosan (Ch), triacontanol (TRIA), and potassium silicate (KSi) on the snap bean cv. Paulista. Over two growing seasons with late sowing and elevated summer temperatures, the research assesses growth, flowering, yield, and quality. Notably, 5 ppm TRIA demonstrates the most significant impact on plant growth and leaf nutrient content. Treatments with 40 ppm 6-BA, 5 ppm TRIA, or 200 ppm KSi exhibit notable effects on inflorescence flower count and flowers per plant. These treatments prove most effective for crucial green pod yield measures, including the number and weight of marketable pods. Moreover, 40 ppm 6-BA or 5 ppm TRIA significantly enhances pod characteristics, such as length, diameter, and weight, consistently improving over both seasons. Particularly, 5 ppm TRIA outperforms in enhancing the chemical quality of pods throughout the study. Overall, the findings suggest that the application of 5 ppm TRIA offers the most favorable enhancements for the growth, flowering, productivity, and quality of snap bean plants in subtropical field conditions.

Bacterial consortia among Bradyrhizobium species, Azospirillum baldaniorum and Bacillus pumilus promote plant growth and efficient symbiotic nitrogen fixation in mung bean

Bacterial consortia among Bradyrhizobium species, Azospirillum baldaniorum and Bacillus pumilus promote plant growth and efficient symbiotic nitrogen fixation in mung bean

Growth, yield, quality and nutrient use efficiency of field bean as influence by planting geometry and nutrient levels

Growth, yield, quality and nutrient use efficiency of field bean as influence by planting geometry and nutrient levels

Effect of seaweed sap (Kappaphycus alvarezii) and micronutrients on growth and yield of moong bean (Vigna radiata L.)

Effect of seaweed sap (Kappaphycus alvarezii) and micronutrients on growth and yield of moong bean (Vigna radiata L.)

Effect of nano-multi micronutrients on growth, quality and economics of French bean

Effect of nano-multi micronutrients on growth, quality and economics of French bean

Effect of deficit irrigation and partial rootzone drying on the water consumptive use, growth and yield of faba bean (vicia faba L.) in a gypsiferous soil

Iraq’s farming sector is under increasing pressure to improve water use productivity as a result of the recent drought, environmental concerns regarding sustainable river ecosystems, and government policies on water metering and allocations. To cope with scarce supplies, deficit irrigation, and partial rootzone drying, defined as the application of water below full crop-water requirements, is an important tool to achieve the goal of reducing irrigation water use. It is necessary to develop new irrigation scheduling approaches, not necessarily based on full crop water requirement, but ones designed to ensure the optimal use of allocated water. These two methods of irrigation are one way of maximizing water use efficiency (WUE) for higher yields per unit of irrigation water applied: the crop is exposed to a certain level of water stress either during a particular period or throughout the whole growing season. The expectation is that any yield reduction will be insignificant compared with the benefits gained through diverting the saved water to irrigate other crops. This paper reviews the yield response of the faba bean (Vicia faba L.). The deficit of irrigation on gypsiferous soils is not known. A field experiment was carried out to study the effect of deficit irrigation and partial drying of the root system on the growth and yield of the bean Randomized Complete Blocks (RCBD) design in gypsiferous soil in the fields of the College of Agriculture- Tikrit University, which is located on Latitude 49° 40ʹ 34˝ North and Longitude 40° 38ʹ 43˝ East At an altitude of 129 m above sea level. The study included seven treatments of full irrigation, deficit irrigation, 25, 50 %, and fixed, alternating partial drying. The seeds of the Barcelona variety were sown for the season on 16/10/2021 and harvested on 2/4/2022. The results showed that the full irrigation treatment gave the highest water consumption use of 635 mm, while the alternative partial root drying decreasing 50% gave the lowest water consumption use of 245 mm and gave the highest crop water use efficiency of 2.0 kg m-3. Treatment full irrigation gave the highest seed yield of 5186.3 kg.ha-1, While the 50% deficit irrigation treatment gave the highest dry weight of the root system, which was 25.6 gm.plant-1.

Photoperiod Regulates Aerobic Methane Emissions by Altering Plant Growth and Physiological Processes

Previous studies have shown that light quality and quantity affect methane emissions from plants. However, the role of photoperiod in plant-derived methane has not been addressed. We studied the effects of two photoperiods—long-day (16 h light/8 h dark), and short-day (8 h light/16 h dark)—on growth and methane emissions of lettuce (a long-day plant), mung bean (a short-day plant), and tomato (a day-neutral plant) under a temperature regime of 22/18 °C. All species were grown under both light durations. First, seeds were germinated in Petri dishes for one week, then plants were transferred to pots and randomly assigned to one of the two experimental conditions. Under each condition, twelve plants were grown for 21 days; at that time, plant growth and physiological traits, including plant dry mass, growth index, photosynthesis, chlorophyll fluorescence, total chlorophyll, nitrogen balance index, flavonoids, and anthocyanin, were measured. Lettuce plants under the short-day photoperiod had the highest methane emissions. Long-day plants that were exposed to short-day conditions and short-day plants that were exposed to long-day conditions were stressed; day-neutral plants were also stressed under short days (p < 0.05). All three species had decreased total dry mass under short-day conditions, most likely because of decreased photosynthesis and increased transpiration and stomatal conductance. Methane emission was positively correlated with shoot/root mass ratio, nonphotochemical quenching and anthocyanin; but was negatively correlated with stem height, dry mass, photosynthesis, water-use efficiency, total chlorophyll, and flavonoids (p < 0.05). This study revealed that, besides light intensity and quality, light duration can also affect methane emissions from plants.

Novel herbicide from an optimized Bacillus altitudinis D30202 solvent extract

AbstractThe herbicidal activity of the fermentation broth, filtrate, and extracts of Bacillus altitudinis D30202 was evaluated against seed germination and seedling growth of wild oat (Avena fatua L.). The bacterial culture filtrate exhibited greater inhibition of the wild oat weed than the fermentation broth. The filtrate was also extracted with organic solvents. The herbicidal potency of the extracts on the growth of wild oat plants was as follows: chloroform (100% inhibition of germination) > n‐butanol (93.8%) > ethyl acetate (66.7%) > petroleum ether (6.3%) > aqueous phase (2.1%); so the chloroform extract was evaluated further. It inhibited wild oat radicle growth by 100%, and plumule growth by 97.9%, while spraying young plants with a 5 mg/mL extract caused severe desiccation of the leaves, stalk wilting, and plant death. IC50 values for inhibition of plumule and radicle growth and germination were 0.64–0.72 mg/mL. Scanning electron microscopy and transmission electron microscopy revealed changes in the microstructure of the leaves and root tips, and degradation of organelles following chloroform extract treatment. The 5 mg/mL extract had no adverse impact on the growth or health of highland barley (Hordeum vulgare L.), corn (Zea mays), and broad bean (Vicia faba L.), indicating that this novel bioherbicide is suitable for control of wild oat weeds in the production of these food crops (while having a minor impact on the health of pea plants and being phytotoxic to wheat plants).

Green synthesis of a dual-functional sulfur nanofertilizer to promote growth and enhance salt stress resilience in faba bean

BackgroundSalinity is a major abiotic stress, and the use of saline water in the agricultural sector will incur greater demand under the current and future climate changing scenarios. The objective of this study was to develop a dual-functional nanofertilizer capable of releasing a micronutrient that nourishes plant growth while enhancing salt stress resilience in faba bean (Vicia faba L.).ResultsMoringa oleifera leaf extract was used to synthesize sulfur nanoparticles (SNPs), which were applied as a foliar spray at different concentrations (0, 25, 50, and 100 mg/l) to mitigate the negative effects of salt stress (150 mM NaCl) on faba bean plants. The SNPs were characterized and found to be spherical in shape with an average size of 10.98 ± 2.91 nm. The results showed that salt stress had detrimental effects on the growth and photosynthetic performance (Fv/Fm) of faba bean compared with control, while foliar spraying with SNPs improved these parameters under salinity stress. SNPs application also increased the levels of osmolytes (soluble sugars, amino acids, proline, and glycine betaine) and nonenzymatic antioxidants, while reducing the levels of oxidative stress biomarkers (MDA and H2O2). Moreover, SNPs treatment under salinity stress stimulated the activity of antioxidant enzymes (ascorbate peroxidase (APX), and peroxidase (POD), polyphenol oxidase (PPO)) and upregulated the expression of stress-responsive genes: chlorophyll a-b binding protein of LHCII type 1-like (Lhcb1), ribulose bisphosphate carboxylase large chain-like (RbcL), cell wall invertase I (CWINV1), ornithine aminotransferase (OAT), and ethylene-responsive transcription factor 1 (ERF1), with the greatest upregulation observed at 50 mg/l SNPs.ConclusionOverall, foliar application of sulfur nanofertilizers in agriculture could improve productivity while minimizing the deleterious effects of salt stress on plants. Therefore, this study provides a strong foundation for future research focused on evaluating the replacement of conventional sulfur-containing fertilizers with their nanoforms to reduce the harmful effects of salinity stress and enhance the productivity of faba beans.

Species interactions and bacterial inoculation enhance plant growth and shape rhizosphere bacterial community structure in faba bean – wheat intercropping under water and P limitations

Grain legumes / cereals intercropping systems with microbial inoculants, hold promise for improving crop productivity under stressful conditions. However, the tripartite interaction involving intercropping system, associated microbiota and stress combining water deficit and low phosphorus (P) availability remains understudied. This study evaluated the impact of three bacterial consortia (C4, C6, and Cref containing Rhizobium and PSB strains) including single Rhizobium (Rhizobium laguerreae) on the agro-physiological performance of wheat (Triticum durum) and faba bean (Vicia faba) grown as intercrops or sole-crops under P and water deficient conditions. Inoculation, especially with C6, significantly improved shoot and root biomasses of both wheat (up to 66 and 81 %) and faba bean (up to 54 and 266 %) intercrops compared to single Rhizobium inoculation and control treatments. Intercropping generally outperformed sole-cropping in above-ground physiology, root morphological traits, shoot and root P content, with a notable effect in response to C6 exhibiting low microbial biomass P. Changes in bacterial community structure were primarily driven by cropping pattern and water regime rather than bacterial inoculation. Intercropping maintained bacterial diversity but shifted community structure, favoring Proteobacteria. Overall, inoculating intercropped wheat and faba bean with Rhizobium-containing consortia induced beneficial below-ground interspecies interactions under water and P-limiting conditions.

Pengaruh Kompos Ampas Tebu terhadap Pertumbuhan dan Hasil Kacang Panjang pada Tanah Alluvial

This study aims to determine the effect of bagasse compost on growth and yield of beans on alluvial soil. This study uses a randomized block designconsisting of 5 treatment, and repeated 5 times, each replication consisted of 5 samples. Those treatments include, k0 without bagasse compost, k1 = 10 tonnes/ha, equivalent to 4.2 kg/plot, k2 = 20 tonnes/ha, equivalent to 8.4 kg/plot, k3 30 tons/ha, equivalent to 12.6 kg/plot, k4 = 40 tonnes/ha, equivalent to 16.8 kg/plot. Variables observed include green level of leaf (SPAD unit), leaf area (cm2), dry weight of the upper part of the plant (g), root volume (cm3), root dry weight (cm3), The number of pods per plant (fruit), length of pods ( cm) and weight of pods per plant (g). Results showed significant effect on the variable observation pod length and pod weight plant however not real effect on the variable observation root volume, root dryweight, leaf area, leaf chlorophyll, and plant dry weightand number of pods.

Synthesize of bio-based encapsulated nano urea modified hydroxyapatite for controlling release of nitrogen and enhancing green bean yield

The massive rise in the world population requires increasing food production, and the world needs to decrease agricultural inputs like agrochemicals to preserve natural resources. The low nutrient use efficiency of conventional fertilizers has always been a concern because of their impact on the environment, and they are considered a waste of natural resources, which is against sustainability goals. Their low efficiency is attributed to their high solubility and fast release into the soil. Controlled-release fertilizers (CRFs) can reduce nutrient loss, which increases their efficiency and controls environmental pollution. In this study, single- and double-layers coating of biopolymers were applied to encapsulate nano urea-modified hydroxyapatite to control nitrogen release in soil. Hydroxyapatite was synthesized using the wet chemical precipitation method and two different rodlike and mesoporous hydroxyapatites were obtained. Nano-hydroxyapatite that had been synthesized was mixed with urea in two different amounts: 4:1 and 8:1. Biopolymers were then added on top. The current CRF synthesis strategy focuses on using low-cost, widespread biorefinery materials to decrease the manufacturing cost of CRFs. The nitrogen release rate of the synthesized CRFs and commercial urea in water and soil was studied. In field experiments, the impact of CRFs on green bean growth and yield was studied. The results showed that both single and double-coated CRFs reduced the N release rate in the soil and increased the fertilizer's longevity to 24 days, compared to 6 days for conventional urea. The total yield of green beans increased by 48%-120% by applying 75% of the recommended dose compared with that obtained with the full dose of conventional urea (control). Also, applying double-coated CRFs at N level of 25% of the recommended dose gives a green bean yield equal to the control. The recommended treatment is SC-CRF prepared with C-HA applied at N rate of 75% to match the future increase in the required amount of food.

Integrating Metarhizium anisopliae entomopathogenic fungi with border cropping reduces black bean aphids (Aphis fabae) damage and enhances yield and quality of French bean

French bean growers, rely mainly on pesticides for pest management. The acceptable tolerance for pesticides residue in French beans is a major concern and has led to several tonnes of the crop continuously rejected and listed as unsafe for human consumption. There is growing demand for alternative approaches and products that are effective at managing pests without the side-effects associated with reliance on pesticides. A field study to determine the combined effects of Metarhizium anisopliae, (Metarril WP E9 and Biomagic) biopesticides and border crops (Sunflower and wheat) on aphid population, damage severity, growth, yield and quality of French bean. A two-factor experiment was conducted at the Egerton University, Kenya. First factor included two border crops (sunflower and wheat) and no border crop (control). Second factor included spraying Metarril WPE9 (2 × 108 cfu/g), Biomagic (2 × 108 cfu/ml) biopesticides, alpha-cypermethrin (synthetic insecticide) and water. Data on growth, yield and quality parameters were collected and analyzed using the SAS version 9.4M8. Results showed that M. anisopliae and border crop significantly (p < 0.0005) enhanced growth, yield and quality of French bean in both seasons. French bean grown with wheat or sunflower borders showed a significant reduction in aphid population (p < 0.0001) and damage severity (p < 0.0001) when sprayed with various treatments compared to the control. Plots with wheat border caused an increase in collar diameter of French bean. The plots (Metarril and wheat border) caused a 4 % and 5 % increase in marketable yield, a 2 % and 12 % reduction in non-marketable yield. To exploit the benefits of biopesticides, the study recommends their integration with and border crops. Thus, French bean growers could benefit more from fungal-based biopesticides in aphid-IPM approach, as it reduces pre-harvest intervals and residues compared to synthetic insecticides.

Offsetting pb induced oxidative stress in Vicia faba plants by foliar spray of chitosan through adjustment of morpho-biochemical and molecular indices

In the course of their life, plants face a multitude of environmental anomaly that affects their growth and production. In recent decades, lead (Pb) gained an increasing attention as it is among the most significant contaminants in the environment. Therefore, in this study the effects of Pb concentrations (0, 50 and 100 ppm) on Vicia faba plants and attempts to alleviate this stress using chitosan (Chs; 0 and 0.1%) were performed. The results validated that with increasing Pb concentrations, a decline in growth, pigments and protein contents was observed. In the same time, a significant upsurge in the stress markers, both malondialdehyde (MDA) and H2O2, was observed under Pb stress. Nonetheless, foliar spraying with Chs improves the faba bean growth, pigment fractions, protein, carbohydrates, reduces MDA and H2O2 contents and decreases Pb concentrations under Pb stress. Pb mitigation effects by Chs are probably related with the activity of antioxidant enzymes, phenylalanine ammonia lyase (PAL) and proline. The application of Chs enhanced the activities of peroxidase, catalase and PAL by 25.77, 17.71 and 20.07%, respectively at 100 ppm Pb compared to their control. Plant genomic material exhibits significant molecular polymorphism, with an average polymorphism of 91.66% across all primers. To assess the genetic distance created among treatments, the dendrogram was constructed and the results of the similarity index ranged from 0.75 to 0.95, indicating genetic divergence. Our research offers a thorough comprehension of the role of Chs in lessening the oxidative stress, which will encourage the use of Chs in agricultural plant protection.

Experimental assessment of biostimulants on mung bean growth on a soilless culture system using superabsorbent pectin based hydrogel

Sustainable agriculture initiatives are needed to ensure the food security of the people all over the world. Soilless cultivation methods using hydrogels may give a revolutionary response as well as a more ecological and productive alternative to conventional farming. This study attempted extraction of pectin from the rind of albedo yellow passion fruit (Passiflora edulis var. flavicarpa Degener)and hydrogels from pectin and activated carbon was compared with pure pectin hydrogel; Pectin- Activated Carbon hydrogels (PAC) showed a microporous structure with excellent hydrophilicity and showed superior water holding capacity. Then the prepared hydrogels were examined with various instrumental techniques like FTIR, SEM, XRD, Raman, BET and rheological properties. In the BET analysis, PAC3 shows the highest surface area of 28.771 m2/g when compared to PAC0 at 15.063 m2/g. The germination experiments were performed using mung beans. This study provides an opportunity for the application of pectin hydrogels in agriculture field specifically for home garden or rooftop cultivation.

Screening and functional verification of drought resistance-related genes in castor bean seeds

Drought is one of the natural stresses that greatly impact plants. Castor bean (Ricinus communis L.) is an oil crop with high economic value. Drought is one of the factors limiting castor bean growth. The drought resistance mechanisms of castor bean have become a research focus. In this study, we used castor germinating embryos as experimental materials, and screened genes related to drought resistance through physiological measurements, proteomics and metabolomics joint analysis; castor drought-related genes were subjected to transient silencing expression analysis in castor leaves to validate their drought-resistant functions, and heterologous overexpression and backward complementary expression in Arabidopsis thaliana, and analysed the mechanism of the genes' response to the participation of Arabidopsis thaliana in drought-resistance.Three drought tolerance-related genes, RcECP 63, RcDDX 31 and RcA/HD1, were obtained by screening and analysis, and transient silencing of expression in castor leaves further verified that these three genes corresponded to drought stress, and heterologous overexpression and back-complementary expression of the three genes in Arabidopsis thaliana revealed that the function of these three genes in drought stress response.In this study, three drought tolerance related genes, RcECP 63, RcDDX 31 and RcA/HD1, were screened and analysed for gene function, which were found to be responsive to drought stress and to function in drought stress, laying the foundation for the study of drought tolerance mechanism in castor bean.

Effect of some nanocomposites on some plant growth indicators. Mung beans (Vigna radiata L.)

An experiment was carried out in the Grain Technology Laboratory, Department of Crops, Faculty of Agriculture - Omar Al-Mukhtar University, during the 2023 AD season, with a completely random design, to study the effect of soaking seeds and spraying seedlings with some Nanoparticles) NPs( (iron, manganese, and copper Nano oxides), Nano-Phosphorus, and Biota agro, and their reflection in some plant growth indicators. Mung beans (Vigna radiata L.) at a concentration of 1 cm/L of one M solution for 10 minutes, then planted in pots with a capacity of 3 kg, with 10 seeds per pot through 3 observations of each treatment. The data showed: • A significant response of mung bean plants to the application of nanoparticles (iron, manganese and copper oxides), nano phosphorus and beta agro was observed in some growth indicators (plant weight, plant height, leaf area, crop growth rate and leaf specific gravity). • Nano phosphorus outperformed by giving the plant the highest weight and leaf area. The crop growth rate reached (3.780 g, 19.2 cm2, 0.506 g/day, respectively) compared to the control treatment. Also, both iron oxide and nano phosphorus outperformed the rest of the treatments under study by giving the highest leaf specific weight. (0.1493, (0.0993) respectively. • Biota Agro excelled in giving the highest plant height, reaching 23 cm, followed by manganese oxide, which reached 21 cm, compared to the control treatment, which decreased to 8.50 cm Keywords: soaking seeds, mung bean growth, Nano-fine fertilizers, Biota agro, Nanoparticles(NPs)

Allelopathy Test of Reeds (Imperata cylindrica) on Germination and Early Growth of Green Beans (Vigna radiata L)

Allelopathy Test of Reeds (Imperata cylindrica) on Germination and Early Growth of Green Beans (Vigna radiata L)