Bean Extract Research Articles

Identification of a β-Carboline Alkaloid from Chemoselectively Derived Vanilla Bean Extract and Its Prevention of Lipid Droplet Accumulation in Human Hepatocytes (HepG2).

Targeting bioactive compounds to prevent lipid droplet accumulation in the liver, we explored an antioxidative extract from vanilla bean (Vainilla planifolia) after chemo-selective derivatization through heating and acid modification. The chemical analysis of vanilla bean extract through chemoselective derivatization resulted in the identification of sixteen compounds (34-50) using LC-MS/MS analysis. A β-carboline alkaloid with a piperidine C-ring and a vanillin moiety at C-1 (34) was identified by molecular networking and diagnostic fragmentation filtering approaches. β-carboline alkaloid 34 exhibited significant inhibitory activity of lipid droplet accumulation (LDAI) in oleic acid-loaded hepatocellular carcinoma HepG2 cells. The LDAI activity was associated with both activation of lipolysis and suppression of lipogenesis in the cells. The study indicates that crude plant extracts, following chemoselective derivatization, may contain bioactive compounds that could be beneficial in preventing hepatosteatosis and could serve as a source of lead compounds for drug development. This approach may be useful to investigate other mixtures of natural products and food resources.

Formulation and evaluation of liquid soap preparation of robusta green coffee extract (Coffea canephora) with virgin coconut oil (VCO) base as an antibacterial Staphylococcus aureus

Staphylococcus aureus can be found on the surface of the skin. One way to prevent Staphylococcus aureus on the skin is to clean the skin with soap. Liquid soap effectively removes dirt from the skin surface and protects the skin from bacteria. Determining the robusta green coffee bean extracts (Coffea canephora) appropriate activity was the study’s major aim in a liquid soap that complies with SNI 06-4085-1996 for use as an antibacterial agent and meets the physical quality requirements for liquid soap preparations. The extraction is performed by a soxhlet extraction method with 96% ethanol. The results of the organoleptic test show a difference in color at each concentration, with the concentration of the extract, which is higher, affecting the color and odor, the results of the homogeneity show results that are homogeneous and free of coarse grains, the results of the pH test show results that meet the requirements, namely in the interval 8-11, the results of the viscosity show results that meet the SNI requirements of 400-4000Cps, the results of the foam height test show stable results and meet the SNI requirements of 13-220 mm. the results of the inhibition zone in the liquid bath soap preparation of robusta green coffee bean extract using the disk diffusion method show that F0 = 4.51mm (resistant), F1 = 11.11mm (resistant), F2 = 13.35mm (intermediate) and F3 = 20.57mm (suspicious).

The addition of Mucuna pruriens seed extract with different dosage on feed conversion ratio and feed efficiency of Rasbora argyrotaenia

The objectives of this research were: 1) determine the effect of feed supplementation using velvet beans (Mucuna pruriens) seed extract with different doses on the feed conversion ratio and feed efficiency of silver barb (R. argyrotaenia), 2) determine the optimal dose of feed supplementation using velvet beans seed extract in silver barb on the value of feed conversion ratio and feed efficiency. This research used an experimental method with a completely randomized design consisting of five treatments of commercial feed without any addition (P0), 0.1 ml/kg velvet beans seed extract addition (P1), 1 ml/kg velvet beans seed extract addition (P2), 10 ml/kg velvet beans seed extract addition (P3), 35 ml/kg velvet seed beans extract addition (P4) in four replication for each treatments. The results of the supplementation with a different concentration of velvet beans seed extract addition showed significant difference (P < 0.05) on the specific growth rate, feed conversion ratio, and feed efficiency of R. argyrotaenia. The highest efficiency and feed conversion ratio of the supplementation of R. argyrotaenia feed obtained from addition of 0.1 ml/kg velvet beans seed extract.

Optimization of microwave assisted extraction of bioactive compounds from black bean waste and evaluation of its antioxidant and antidiabetic potential in vitro

Legumes processing industries have led to an accumulation of waste as a byproduct which is often discarded. However, this study aimed to use a ‘green technology’ such as microwave-assisted extraction (MAE) to valorize the wastage and assess the functional attributes of black beans (Phaseolus vulgaris L.) hull powder (BBHP). The Box- Behnken design was employed to optimize the response variables of MAE and the process variables were as follows: microwave power (100–600 W), extraction time (2–6 min), and solvent: solid ratio (20:1–50:1 mL/g). For comparative purposes, conventional solvent extraction (CSE) has been considered for extracting bioactive compounds. The black bean extract was obtained by MAE under optimum conditions of microwave power of 334.4 W, solvent: solid ratios of 50:1 mL/g, and an extraction time of 6 min showed the highest yield of bioactive components, with total anthocyanin and phenolic content of 34.14 mg/g and 197.23 mg (GAE)/g, and their antioxidant activities are 91.37 % ABTS and 93.51 % DPPH compared to CSE. Further, an optimized sample of BBHP extracts exhibited the inhibitory effects on the activities of α-amylase 80.41% and α-glucosidase 86.14% respectively. Overall results show that MAE is better than CSE for extraction of functional attributes from black bean waste.

EFFECTS OF LIMA BEAN (Phaseolus lunatus) FLOUR ON COGNITIVE FUNCTION AND GROWTH RECOVERY MALNUTRITION RATS

Incorporating lima bean (Phaseolus lunatus) flour can replace and complement the content of proteins, macronutrients, and micronutrients, which may be related to curative effects for malnutrition. Therefore, an in vivo approach was undertaken to demonstrate the effect of lima bean flour on cognitive function and growth recovery in malnourished rats. Four groups of male Wistar rats were included in this study: The control group (N), MAL, MAL+25% LB group, and MAL+50% LB group. Morphometric analysis was used to assess growth recovery. Cognitive tests included Y-Maze, Novel Object Recognition Test (NORT), and Water Maze by Morris (MWM) test, and histopathological examination of the brain sections was conducted on the cerebral cortex and hippocampus by hematoxylin and eosin stains. The biological activity prediction of lima bean active compounds was analyzed by the PASS server using an in-silico study. This study found that lima bean flour increased body weight, length, tail increment, ear length, and leg length in malnourished rats at 50% lima bean concentration, similar to the control group. Lima bean flour showed neuroprotective capabilities by reversing the effects of malnutrition on the cerebral cortex and hippocampus by lowering the percentage of degenerated cells and increasing cognitive and behavioral. Eight bioactive compounds in lima bean extract have antioxidant, anti-inflammatory, and antibacterial properties. Lima bean flour, a source of complex nutrients, protects against malnutrition systemically in various organ systems, including the brain.

The Antioxidant and Antidepressant Properties of Dietary Proteins Derived from Egg and Bean Extracts and Their Acute Toxicity: A Journey from Nutrition to Pharmacognosy

This research reveals the previously unexplored pharmacognostic potential of antidepressants found in nutrients derived from both legume and animal sources. Through preclinical investigations involving mouse models, the study focused into antidepressant and antioxidant activities of non-denatured and denatured protein extracts from beans and eggs. Non-denatured protein extracts from beans and eggs, at saturation levels of 40% and 80%, were examined as macronutrients, while denatured protein extracts at equivalent saturation levels were considered micronutrients. The study employed the DPPH and hydrogen peroxide tests to assess antioxidant activity, and the forced swimming test and sucrose preference test to evaluate acute and chronic mild antidepressant effects, respectively. The acute toxicity study revealed that macronutrients from eggs at 40% and 80% saturation displayed non-toxic effects (LD50 &gt;5 g/kg), while those from beans, specifically at saturation of 80%, exhibited a relatively low level of toxicity (LD50 = 2.5 g/kg). Evaluation of antioxidant activity using the DPPH test yielded inconclusive results due to the influence of ethanol precipitation. In contrast, the H2O2 test demonstrated significant antioxidant potential in both macronutrients and micronutrients extracted from beans and eggs at all saturation levels. In investigating antidepressant properties, both macronutrients and micronutrients of bean and egg protein extracts at 40% and 80% saturation exhibited notable antidepressant effects, particularly the micronutrients at saturation of 80%. This antidepressant effect was characterized by a reduction in immobility time and an increase in sucrose preference.

Effect of Thermal Processing and Oxygen concentration on the Phenolic Composition and Inhibition of Advanced Glycation End Products of Chilean Beans (Phaseolus vulgaris) Extracts

Effect of Thermal Processing and Oxygen concentration on the Phenolic Composition and Inhibition of Advanced Glycation End Products of Chilean Beans (Phaseolus vulgaris) Extracts

Individual Growth And Gonad Development Of Female Tilapia (Oreochromis Sp.) With Addition Of Velvet Bean (Mucuna Pruriens) Seed Extract

Tilapia is one of the fish that is widely cultivated in Indonesia. The obstacle to tilapia cultivation is high reproduction, which inhibits growth. An alternative to increase growth is by inhibiting gonad growth, so that feed energy is focused on somatic development. The aim of this research was to analyse the effect of velvet bean extract concentration on individual growth and gonad development of female tilapia fish and to determine the optimum concentration of velvet bean extract to increase growth and reduce reproductive activity in female tilapia fish. This study used a Completely Randomized Design (CRD) with the addition of various velvet bean extracts and compared with the control (without velvet bean extract). The use of velvet bean extract had a significant effect (p&lt;0.05) on growth parameters such as length, weight, fecundity and egg diameter. The best treatment was found in treatment 3 (velvet bean extract 5 ml.kg-1 ) which resulted in growth in individual length of 16.1 cm, individual weight of 89.43 g, fecundity of 2206 and egg diameter of 1.11 mm. In addition, the dose in this treatment may reduce the reproductive activity of tilapia.

Enhancing Bioactivity and Conjugation in Green Coffee Bean (Coffea arabica) Extract through Cold Plasma Treatment: Insights into Antioxidant Activity and Phenolic-Protein Conjugates.

Cold plasma technology is gaining attention as a promising approach to enhancing the bioactivity of plant extracts. However, its impact on green coffee bean extracts (GCBEs) still needs to be explored. In this study, an innovative underwater plasma jet system was employed to investigate the effects of cold plasma on Coffea arabica GCBEs, focusing on the conjugation reflected by the change in composition and bioactivity. The DPPH radical scavenging antioxidant activity exhibited a gradual increase with plasma treatment up to 35 min, followed by a decline. Remarkably, at 35 min, the plasma treatment resulted in a significant 66% increase in the DPPH radical scavenging activity of the GCBE. The total phenolic compound content also displayed a similar increasing trend to the DPPH radical scavenging activity. However, the phenolic profile analysis indicated a significant decrease in chlorogenic acids and caffeine. Furthermore, the chemical composition analysis revealed a decrease in free amino acids, while sucrose remained unchanged. Additionally, the SDS-PAGE results suggested a slight increase in protein size. The observed enhancement in antioxidant activity, despite the reduction in the two major antioxidants in the GCBE, along with the increase in protein size, might suggest the occurrence of conjugation processes induced by plasma, particularly involving proteins and phenolic compounds. Notably, the plasma treatment exhibited no adverse effects on the extract's safety, as confirmed by the MTT assay. These findings indicate that cold plasma treatment holds significant promise in improving the functional properties of GCBE while ensuring its safety. Incorporating cold plasma technology into the processing of natural extracts may offer exciting opportunities for developing novel and potent antioxidant-rich products.

Monitoring of Chemical Changes in Coffee Beans during the Roasting Process Using Different Roasting Technologies with Nuclear Magnetic Resonance Spectroscopy

The roasting process is an important step in coffee production, leading to important physical and chemical changes that are responsible for the sensory quality of a coffee beverage. Besides the commonly used drum roasters, a newly developed infrared roaster can be used to roast green coffee beans. In this study, 1H nuclear magnetic resonance (NMR) spectroscopy was used to analyze the fat and aqueous extracts of coffee beans roasted to different degrees of roasting using a professional drum roaster, a hot air fluidized bed sample roaster and an infrared roaster. Caffeine-containing and decaffeinated Coffea arabica coffee samples were used to monitor the roasting process in the different roasters. Compared with the drum-roasted coffee sample, the formation and degradation of NMR-detectable components in the coffee sample roasted with the infrared roaster and the hot air roaster were time-dependent. In the decaffeinated coffee sample, compounds such as kahweol, caffeoylquinic acid and trigonelline were found to occur at lower levels. The formation and degradation of the NMR-detectable compounds in the decaffeinated coffee sample also occurred with a time lag or to a lesser extent than in the caffeine-containing coffee sample.

Karakteristik Organoleptik Permen Jeli Sari Kacang Merah dengan Penambahan Gelatin Tulang Ikan Nila Merah

This study aimed to determine the organoleptic characteristics of red bean extract based on jelly candy with the addition of nile bones gelatin. This study used a completely randomized design with factorial experiments. The first factor is Nila-bones gelatin concentration which consists of concentration levels A1 (10 g) and A2 (13 g). The second factor was the addition of red bean extract to replace water which consisted of 3 levels, namely B1 (50%), B2 (70%), and B3 (100%). Data analysis of organoleptic used the Kruskal-Wallis test. When p &lt; 0.05, will be continued by the Mann Whitney test at the real level α 5%. The results showed that the addition of Nila-bones gelatin to jelly candy based on red bean extract had a significant effect (p &lt; 0.05) on the quality and level of overall panelist preference. A1B2 (10 g of gelatin; 70% of red bean extract) is the formula for the selected jelly candy red bean extract.

Performance comparison of anthocyanin-based smart indicator films

Anthocyanins are natural dyes from plants that change color according to environmental changes, allowing for their potential use in real-time freshness-indicator films. In this study, black rice extract (BRE), black soya bean extract (BSBE), black wolfberry extract (BWE), purple sweet potato extract (PSPE), purple cabbage extract (PCE) and roselle extract (RE) were added to soybean isolate protein/chitosan (SPI/CS) films, and their performance was assessed. Results showed the total anthocyanin content of these six extracts were 1.50, 1.13, 1.98, 0.47, 0.33, 2.67 mg/mL, respectively. The presence of the six major anthocyanins was confirmed by mass spectrometry. Spectroscopy revealed that anthocyanin addition did not induce interactions between the extracts and SPI/CS film. Excess of anthocyanins weakened interactions between macromolecules, damaged membrane structure and reduced mechanical properties. Membrane water barrier properties improved with an increase in anthocyanin content, as well as antioxidant properties, but the difference between the indicator films was not significant. Finally, SPI/CS/RE exhibited a significant color change and the sensitivity was up to 50.6%, indicating the best application performance.

EDUKASI PEMBERIAN SARI KACANG HIJAU (VIGNA RADIATE) SEBAGAI UPAYA PENINGKATAN PRODUKSI ASI PADA IBU NIFAS

Exclusive breastfeeding refers to the practice of providing breast milk solely to infants aged 0-6 months without introducing any additional solid foods or liquids such as formula, fruit juice, honey, or tea. The objective of this study is to assess the impact of administering green bean juice (Vigna Radiate) on enhancing postpartum breast milk production among mothers receiving care at the Yeni Clinic in the Tinggi Raja District, Asahan Regency, in the year 2022. This research follows a quantitative approach and employs a quasi-experimental design known as the "slum experiment," involving both pre-test and One Group post-test designs. Data analysis involved the application of a paired t-test on a sample of 10 postpartum mothers facing challenges in providing exclusive breastfeeding, and the study was conducted in June 2022. The findings of the study indicated that prior to the introduction of green bean extract, the majority of mothers (90%) experienced low breast milk production, with only one mother (10%) achieving sufficient breast milk production. Following the reintroduction of green bean extract, two mothers (20%) observed an increase in breast milk production, while eight other mothers (80%) achieved adequate breast milk production. Data analysis results revealed a p-value of 0.000, which is less than 0.005, indicating a significant influence of administering green bean juice (Vigna Radiate) on breast milk production among postpartum mothers at the Yeni Clinic, Tinggi Raja District, Asahan Regency, in 2022.

Efficacy and safety of Vicia faba L. extract compared with levodopa in management of Parkinson’s disease and an in‐silico phytomedicine analysis

Background: Parkinson’s disease is a chronic degenerative disease of the central nervous system, presently lacks an effective therapy for its complex pathogenesis. Agents containing Levodopa can alleviate its symptoms. Hypothesis Vicia faba L. (Fava bean) extract may prove a useful antiparkinsonian agent similar to Levodopa. Methods: Thirty patients with Parkinson’s disease, entered into this cross over clinical study. In the first step, each participant received V. faba L. extract containing 106.5 mg of Levodopa. After a wash out period of 7 days, the patients entered the second step during which they received conventional treatment with Levodopa-C tablets. Blood Levodopa were measured 4 hours and 8 hours after each administration. The Movement Disorder Society-Unified Parkinson’s Disease Rating Scale was employed to measure the therapeutic effects in each step. Additionally, a docking analysis was performed to distinguish the chemical constituents of the plant and six key mediators actively involved in Parkinson disease. Results: The fava bean extract significantly alleviate all studied end points except for tremor at rest and freezing. Wilcoxon Signed Ranks Test showed that there was not a significant difference between rigidity, rising chair, gait and bradykinesia. Docking results of the herb components and standard ligands, indicating that the antiparkinsonian activity of V. faba could presumably be related to many phytochemicals. Conclusion: This study showed a beneficial effect of V. faba L. similar to Levodopa-C. and better compliance due to lower adverse events.

Expression of TNF-α and TGF-β after the Administration of Cacao Bean Extract on Alveolar Bone

Background: Teeth that are given orthodontic forces can relapse because of excessive resorption on the pressure side. Cocoa bean extract enhances bone apposition during bone remodeling by increasing osteoblast proliferation. This research aimed to ascertain molecular mechanisms of alveolar bone during orthodontic tooth movement applied with cacao bean extract (Theobroma cacao L.) through TNF-α and TGF-β, expressions. Methods: This study utilized an experimental laboratory posttest-only control group design. The NiTi closed coil spring was braced between the right upper first molar and the upper incisor, a strength of 10gf was estimated utilizing a tension gauge to mesially move the upper molars. The total 36 were divided into six groups, Wistar rats were beheaded on days 7 and 14 of treatment. Immunohistochemical staining was utilized to show TNF-α and TGF-β expression. Results were assessed using the one-way ANOVA analysis. Results: The immunohistochemistry findings in osteoclast cells that demonstrate positive results for TGF-β and TNF-α, expression by immunohistochemical staining calculated for each group (control, positive control, treatment). The treatment group (Q14) showed reduced expression (2±0.957) of TNF-α in osteoclast cells. TGF-β, on the other hand, was found with diminished expression in osteoblast cells C-7 and C-14 (3.75±0.816, 3.25±0.957) and increases in the treatment group (Q14) (11.25±0.957). significantly (p=0.000). Conclusion: The administration of cacao bean extract on days 7 and 14 could decrease the expression of TNF-α and increase TGF-β of the treatment group. Therefore, with an increased TNF-α apposition process, relapse after orthodontic treatment can be prevented to accelerate the orthodontic treatment.

EFFECT OF ROBUSTA COFFEE BEANS AND ARABICA EXTRACT GEL AS INFLAMATION PULP MATERIAL

Background: Reversible pulpitis is a mild to moderate inflammatory pulp condition often treated with pulp capping treatments. Current study states that calcium hydroxide (CaOH2) has drawbacks for a long-time use. One of the alternative pulp capping materials is Robusta and Arabica coffee bean extract gel which contains flavonoid compounds and chlorogenic acid which have immunomodulatory properties that play role in the pulp inflammatory process by increasing the count of lymphocyte. Objective: The goal of this study is to determine and analyse the effect of Robusta coffee bean and Arabica coffee bean extract gel 95% to the of lymphocyte cells count on the 3rd and 5th day of pulp inflammation compared to placebo gel. Materials and Methods: This research is a true experimental with a post-test only control group design. This study used three treatment groups; Robusta coffee bean extract gel 95%, Arabica coffee bean extract gel 95%, and control group placebo gel. Results: Two Way Anova test results obtained p&lt;0.05, showed significant difference. Data analysis was continued with the Post Hoc Bonferroni test which showed that there was a significant difference between the 95% Robusta coffee bean extract gel group and the 95% Arabica coffee bean extract gel group with p&lt;0.05. Conclusion: The administration of Robusta coffee bean extract gel with a concentration of 95% can increase the of lymphocyte cells count in pulp inflammation on the 3rd and 5th days more effectively than the 95% Arabica coffee bean extract gel. Keywords: Arabica Coffee, Direct Pulp Capping, Inflammation, Lymphocytes, Pulpitis, Robusta Coffee

Antioxidant Activity of Fermented Red Bean Extract on Sperm Quality of Mice Exposed to Cigarette Smoke

Smoking has a negative effect on fertilization as it decreases sperm quality. The current research aimed to investigate the effect of fermented red bean (Phaseolus vulgaris L.) extract on sperm motility, viability, and plasma membrane integrity of white mice (Mus musculus) exposed to cigarette smoke. The red beans were subjected to a 36-hour fermentation process using Rhizopus spp. Then, methanol was extracted by maceration method for 24 hours until maceration was obtained. In this study, 25 male white mice aged 3 months were randomly divided into 5 groups of 5 mice. Group C (negative control) was given 0.5 mL of carboxymethyl cellulose natrium (CMC Na) 1% solution orally without unfiltered cigarette smoke exposure, and group C+ (positive control) was given 0.5 mL of CMC Na 1% solution orally and unfiltered cigarette smoke exposure. Treatment groups T1, T2, and T3 were orally given fermented red bean extract at doses of 26 mg/kg Body weight (BW), 52 mg/kg BW, and 104 mg/kg BW, respectively, and then were exposed to unfiltered cigarette smoke. For 36 days, treatment groups (except the negative control) were subjected to the inhalation of smoke from an unfiltered cigarette containing a nicotine dose of 2.2 mg. The exposure period lasted for 20 minutes each day. Each group was put into a cigarette smoke-exposing box. The sperm motility (observing the forward movement of spermatozoa), the sperm viability (examining the color of the sperm head), and the sperm plasma membrane integrity (observing the tail shape using the hypoosmotic swelling test) were then evaluated. The findings indicated significant differences in sperm motility, viability, and plasma membrane integrity of each group with positive control. A dose of 104 mg/kg BW of fermented red bean extract had the best potential to maintain sperm motility (70%), viability (82.13%), and plasma membrane integrity (61.93%) of mice exposed to unfiltered cigarette smoke.

The use of Green Bean Juice as an Effort to Improve MSCT Image in Abdomen MSCT Protocol (in Vitro Study)

Oral contrast media usually used in abdominal MSCT examinations are water and contrast media containing iodine. However, there are advantages and disadvantages, so an alternative replacement for natural ingredients is needed, namely green bean juice. This study aims to prove that using mung bean extract can improve MSCT images for oral contrast media in the MSCT Abdomen protocol based on in vitro studies. The research method used was a quasi-experimental study to compare the extent to which mung bean extract improved MSCT images compared to water and contrast media containing iodine as oral contrast media in the Abdomen MSCT protocol based on in vitro studies. Measurement of image enhancement is done by measuring ROI. Furthermore, the measurement results were analyzed using the difference test and a follow-up test, namely the Least Significance Difference (LSD) test. The research results show that green bean juice can improve abdominal MSCT images to be used as an oral contrast medium in the Abdominal MSCT protocol based on in vitro studies. Then, there was a difference in improving the MSCT image when using green bean juice, water, and contrast media containing iodine as oral contrast media in the abdominal MSCT protocol based on in vitro studies, and it was found that there was a difference in the concentration of green bean juice in getting optimal results in the MSCT protocol. Abdomen based on in vitro studies. Conclusion Mung bean extract can be used as an oral contrast medium instead of water because it can improve MSCT images.

The Role of Robusta Green Bean Extract in Decreasing Il-1β Levels on Dental Pulp Stem Cells (Dpsc) Induced by Lps Porphyromonas Gingivalis

Background: Stem cell-based tissue engineering is a potential strategy for periodontal tissue regeneration. Besides the advantages of stem cells as periodontal tissue regenerative therapy, this therapy must also be able to prevent and control the possibility of inflammation that occurs. Robusta green beans contain anti-inflammationsubstances that may decrease excessive inflammation in stem cell therapy. Purpose: To analyze IL-1β levels after giving Robusta green bean extract to DPSC induced by LPS P. gingivalis. Materials and Methods: This study used DPSC from the extraction of premolar teeth of orthodontic patients. The viability test was performed on the LPS P. gingivalis at 0.5; 1; 5; 10µg/ml and robusta green bean extract at 0.0625%; 0.125%; 0.25%; 0.5%to determine non toxic concentration. The treatment group was divided into 4 groups, the control group, the DPSC+LPS group, the DPSC + LPS + 0.125% Robusta green bean extract group, and the DPSC+LPS+0.0625%Robusta green bean extract group.Each group was observed at 24, 48, and 72 hours. ELISA test was performed on the supernatant to measure IL-1β levels. Results: The level of IL-1β in the Robusta green bean extract group was significantly lower (p &lt;0.05) than the group without Robusta green bean extract at all observation times. Conclusion: Robusta green bean extract concentrations of 0.125% and 0.0625% can significantly reduce IL-1β levels in DPSC induced by LPS P. gingivalis, which is the most effective at reducing IL-1β levels was 0.125%.

Optimization of Defective Coffee Beans Decaffeination Using Palm Oil

Defective coffee beans amount to 15-20% of the total produced coffee beans. The defective coffee bean contains caffeine, which can negatively affect the human body, such as increased heart rate, and thus sensitive to consumption by some people. This study aims to optimize the decaffeination process of defective coffee beans. The extraction of aroma and flavor compounds was done by maceration, and the decaffeination was carried out using palm oil as a solvent. The type of beans (green and roasted beans), the decaffeination contact time, and the ratio between coffee bean extract and solvent were varied in this study. The caffeine content was quantified, and the organoleptic and color tests were done on the concentrated coffee extracts. It was found that the higher the amount of solvent volume in decaffeination, the higher the caffeine decrease. In addition, the longer the green beans’ decaffeination time, the lower the caffeine decrease. Decaffeination using green coffee beans resulted in a greater reduction of caffeine (6.515-48.241%) than roasted coffee beans (8.495-24.272%). The optimum operating condition of green coffee bean decaffeination was the coffee bean extract and solvent ratio of 1:5.82 and the decaffeination time of 26.5 minutes. The organoleptic test result shows that decaffeinated coffee flavor had the same preferability as the commercial coffee flavor and was thus able to compete in the market.