Baung Fish Research Articles

Domestication of Asian Redtail Catfish (Hemibagrus sp.) as an Effort to Preserve Local Fish at the Loka Ngrajek in Magelang, Central Java

The Baung fish (Hemibagrus sp.) were used in this research were the first domesticated breed of native fish from Elo river, Magelang, Central Java. The growth pattern of Baung fish is very important to know for management and spesies conservation. The aim of this research were to analyze the size distribution, growth patterns and condition factors of Baung fish as a basis for managing fish resources. The research was conducted in September 2023 at Loka Ngrajek Magelang, Central Java. The fish samples used were the result of first generation domestication which were cultived in concrete tanks for six months. The body weight of Baung fish ranged from 66 g to 330 g. The survival rate of Baung fish from 5-7 cm to 20-35 cm were 55 ± 2.7%, while when enlargement it reached 93 ± 3.6%. Loka Ngrajek succeeded in domestication and produced the first generation with daily fish growth of 2.65 ± 0.11%. Cultivated fish have a length-weight relationship model W = 0.0104X2.9414, which showed negative allometric meaning that the increase in length was faster than the increase in weight.

Analisis Hasil Tangkapan Di Perairan Umum Daratan Krueng Aceh

Aceh has considerable potential for inland public aquatic resources. One of the important inland waters is Krueng Aceh, Krueng Aceh is a river located in Aceh Province in Cot Seukek, Aceh Besar Regency, passing through the city center (Peunayong) and empties into Lampulo Banda Aceh City. Analysis of catches in Krueng Aceh was carried out with the aim of determining the types of fish in the public waters of the Krueng Aceh mainland as basic data in the management of mainland public water resources. The study used the Purposive sampling technique at four stations and the results showed that there were 9 types of fish in the public waters of the Krueng Aceh mainland which were dominated by baung fish. The station with the most catches is station II located at Bakoy Village, Ingin Jaya District, Aceh Besar District. There are 4 types of fishing gear that are commonly used in the public waters of the Krueng Aceh mainland and gill net fishing gear is the most effective fishing gear used.

Keanekaragaman Ikan Invasif Di Perairan Umum Daratan Krueng Aceh

Aceh has a large potential for inland public water resources. One of the important inland waters is Krueng Aceh, Krueng Aceh is a river located in Aceh Province that originates at Cot Seukek, Aceh Besar District, passes through the city center (Peunayong) and empties into Lampulo, Banda Aceh City. The diversity of invasive fish in the inland waters of Krueng Aceh was carried out with the aim of identifying the types of invasive fish and to determine the diversity of invasive fish in the waters of Krueng Aceh as basic data in the management of inland common water resources. The research used a purposive sampling technique at sample points taken at 4 (four) points on the mainland of Krueng Aceh. Station 1 is located in Aneuk Galong Village, Station 2 is located in Bakoy Village, Station 3 is located in Pango Raya Village, Station 4 is located in Peunayong Village. The research results show that there are 9 types of fish in the public waters of the Krueng Aceh mainland which are dominated by baung fish. The station with the highest diversity results is station III located in Pango Raya which uses 4 types of fishing gear commonly used in the public waters of the Krueng Aceh mainland and gill net fishing gear is the most effective fishing gear used.

UJI PERENDAMAN KOMBINASI LARUTAN TEPUNG KUNYIT DAN CACING SUTERA (Tubifex sp) TERHADAP SINTASAN DAN PERTUMBUHAN BENIH IKAN BAUNG (Hemibagrus nemurus)

This study aims to determine the effect of the long soaking of silkworms (Tubifex sp) in turmeric flour solution on the dilution and growth of baung fish fry (Hemibagrus nemurus). The method used is a Complete Randomized Design (RAL) with 5 treatments of 3 tests, namely P0 treatment = no soaking, P1 = Soaking for 20 minutes, P2 = 25 minutes, P3 = 30 minutes and P4 35 minutes. The baung fish used are fry that are 8 days old with an average weight of 0.09 gr / tail with a length of 0.70 cm / tail. The seeds were obtained from Saprizal's UPR in Padang Mutung village, Kampar district. From the results of the study, the highest dilution was obtained in the P4 treatment by 100% and the lowest in P0 by 97.33%. The highest absolute weight growth of P4 treatment was 0.57 gr and the lowest was 0.37 gr. The highest absolute length growth in the P4 treatment was 3.46 cm and the lowest was 2.99 cm. The highest daily growth rate of P4 treatment was 0.08% and the lowest was 0.05%. The best feed conversion value is P4 treatment of 0.463%. Water quality parameters are temperature 28-30 0C, pH 6.13-7.69, dissolved oxygen 3.4-6.3 mg/L and Ammonia 0.88-4.22 mg/L.

STRUKTUR KOMUNITAS IKAN HASIL TANGKAPAN JARING INSANG DI SUNGAI BATANG TEBO DESA BUNGKAL KECAMATAN TEBO TENGAH KABUPATEN TEBO

One of the largest tributaries in the Batanghari area is the Batang Tebo River which passes through Bungkal Village. Currently, the Batang Tebo River is a place for waste disposal, garbage, oil spills, and even the biggest illegal gold mining (PETI). This resulted in a drastic decrease in fishermen in Batang Tebo waters using gill nets. The purpose of the study was to determine the structure of the fish community in Batang Tebo from the catch when using gill nets. This research was started on April 18, 2022 - May 31, 2022, and is located on the Batang Tebo River, Bungkal Village, Tebo Regency. The researcher thus causes the survey method and random sampling as the data collection. Collection of data from this research activity included the structure of the community caught by fishermen using gill nets. The catch data includes fish size, fish weight, total fish weight, and the fish species. The supporting data collect environmental parameters (temperature, pH, current speed, brightness). The number of fish catches produced during the research activity reached 292, with the composition of the catch consisting of 5 species, namely: Baung Fish (Hemibagrus Menerus), Sand Lambak Fish (Labiobarbus Festivus), Lais Fish (Cryptopterus Sp), Kapiat Fish (Barbonymus schwanenfeldi), Juaro Fish (Pangasius polyuranodon). The highest catch was Baung fish many as 89 fish lowe catch was Juaro fish as many as 17 dominant index was 0.23. Community structure of fish caught by Baung Fish, Flat Lambak Fish, Lais Fish, Kapiat Fish, and Juaro Fish. Community structure of fish caught by Baung Fish, Flat Lambak Fish, Lais Fish, Kapiat Fish, juror fish. With diversity with medium, uniformity with (high), on dominance with (low), there are fish that dominate in the catch and are also supported by environmental parameters in the form of temperature, pH, brightness, and speed of current.

Study of Genetic Diversity of Native Indonesian Catfish Based on Nucleotide Sequences of the ND1 Gene

One of the native catfish to Indonesia is the baung fish (Hemibagrus). Baung fish are found in rivers in Sumatra, Kalimantan and Java. The catfish population is declining, thus conservation is needed to prevent extinction. To conduct an effective conservation efforts, molecular studies are needed to confirm the fish species from the three islands. Mitochondrial DNA is one of the markers that is often used to see the lineage and kinship of animals for conservation purposes. The purpose of this study is to determine the genetic diversity of the NADH Dehydrogenase Subunit I (ND1) gene catfish from each of these locations, can be used as a genetic marker. Samples were obtained from their natural habitat, namely Magelang (5), Palembang (3), Riau (2), Samarinda (2), 2 from Sintang, and 3 from Banjarmasin. The DNA of the fish sample was then isolated and then used as a template for amplification of DNA fragments using PCR techniques. Amplikon (PCR product) was then purified by gel extraction and then sequenced to determine the DNA sequence. The potential of DNA sequences as catfish genetic markers was proven by analyzing genetic diversity between species using the MEGA version 7.0 program (Kumar et al., 2016). The sequencing results 972 nucleotides composing the ND gene, and found differences in 268 nucleotide sites and 47 amino acid sites. Based on the nucleotide sequence of the ND1 gene, catfish from the Progo river (Magelang, Central Java), the Musi river (Palembang, Sumatra), the Kampar river (Riau), the Kapuas river (Sintang, Kalimantan), the Martapura river (Banjarmasin), the Mahakam river (Kalimantan) is included in the Hemibagrus sp.; catfish from the Elo river (Magelang, Central Java) belongs to the genus Mystus sp.; catfish from the Bengawan Solo river (Bojonegoro, East Java) belongs to the Pangasius sp.

Sustainability Status of Baung Fish in Public Waters, Kampar Regency, Riau Province

This study aims to assess the sustainability status of the utilization of baung fish (Mystus nemurus) in the public waters of Kampar Regency. The study was conducted in January 2022 in the public waters of Kampar Regency. The data used consists of primary data and secondary data, while the analysis was carried out by RAPFISH method. The dimensions used in this study consist of ecological, economic, social, institutional and technological. The results of the analysis show that the utilization of baung fish resources in the public waters of Kampar Regency is less sustainable with a multidimensional index value of 46.21 percent. The sustainability status in this less sustainable category requires the intervention of stakeholders in management can warrant the sustainability of baung fish in Kampar Regency.

Pusat Penelitian dan Pengembangan Budidaya Perikanan dengan Pendekatan Arsitektur Biophilic

Kampar Regency is one of the regencies with a quit spacious aquaculture area with pisciculture business activities as one of the livelihoods of most of the people. Freshwater fish farming in Kampar Regency includes the pisciculture of karamba or in ponds. The request for freshwater fish production in Riau province is also quite high, such as catfish, carp, tilapia, pomfret and baung fish for daily consumption or industrial needs. The high request for fish production in Kampar regency cannot always be fulfielld. This is due to the failure of aquaculture caused by various factors such as climate, environment, pests and diseases. So to avoid the occurrence of these failures required skilled human resources and adequate facilities. Research and Development Center for Aquaculture which functions as a center for development, research and public education in advancing the community's economy, which includes a research center, laboratory and also commercial functions in the fisheries sector. In designing the Center for Aquaculture Research and Development, the Biophilic architectural approach is used. Biophilic architecture is a good relationship between nature, humans and architecture. The concept of the building takes the concept of "River Water Flow" as an implementation of natural formations by considering the three aspects ofarchitecture Biophilic into a single unit.

Identifikasi Endoparasit Pada Ikan Hasil Tangkap Sebagai Kandidat Ikan Domestikasi di Danau Lais Kota Palangka Raya

The study aimed at determine the type of endoparasites in catch fish in Lake Lais Palangka Raya, Central Kalimantan which will later be used as candidate fish for domestication so that it can be used as a basis for determining attitudes and the next step in overcoming problems encountered in aquaculture activities examined at the Parasitology Laboratory Station. Fish Quarantine Quality Control and Safety of Fishery Products Palangka Raya. Examination of endoparasites is done by observing the internal organs by making a mucus smear which is examined under a microscope. From the results of the study, it was found that the dominant catches from the waters of Lake Lais were Toman fish (Channa micropeltes), Baung fish (Mystusnemurus), Gabus fish (Channa striata), Kerandang fish (Channa pleurophthalma) and 7 types of parasites were found in the caught fish. Dominating the waters of Lake Lais, namely Henneguya sp, Myxobolus sp, Senga sp, Philometra sp, Neoechinorhynchus sp, Cammalanus sp, Echinostoma sp. The highest prevalence value in captured fish from Lake Lais is found in Toman fish, which is 100%, followed by Kerandang fish 90%, Cork fish 70% and the lowest prevalence value is in Baung fish, namely 50%, as well as the intensity value in fish. Toman is taller than other caught fish.

Jenis-Jenis Ikan di Sungai Desa Murung Panti Hilir Kecamatan Babirik

This study aims to identify what fish are found in Murung Panti Hilir Village. The type of research carried out is field research, namely research carried out by collecting data in the field. The data collection technique in sampling is purposive sampling, which is used to determine the research location point. While the sampling technique used the Line Transect method in the swamp area of the village of Murung Panti Hilir. Sampling is done by catching fish found using fishing nets or lunta tools. Installation of tools is carried out in the morning and afternoon. Each sample that is brought one by one will be documented at the place where the sample was found. After that, each sample was made into clear plastic and labeled. From the results of the research conducted, there are 12 types of fish in Murung Panti Hilir Village, Babirik District, namely Tapah Fish (Wallago leeri), Tawes Fish (Puntius javanivus), Saluang Batu Fish (Rasbora aurotaenia), Timah-Timah Fish (Panchax-Panchax), Julung-Julung Fish (Zenarchopterus buffoni), Puyau Lamah Fish (Osteochilus vittatus), Papuyu Fish (Anabas tesrudineus), Lais Tembiring Fish (Crytopterus sp.), Kelabau Fish (Osteochilus kelabau), Baung Fish (Macrones nemurus), Sanggaccompani Fish (Macroness nigriceps) ), and Nila Fish (Oreochromis niloticus).

POLIMORFISME GEN MITOKONDRIA 16S IKAN BAUNG (Hemibagrus nemurus Valenciennes, 1840) DARI SUNGAI PROGO, MAGELANG, JAWA-TENGAH

Abstract: Asian redtail catfish (Hemibagrus nemurus Valenciennes, 1840) is one of Indonesian native fish species with high economic potency. However research on genetic variation of Asian redtail catfish in Indonesia is very limited. Therefore, this study purposed to identify and analyze the mitochondrial gene polymorphism 16S of Baung fish (H. nemurus) from the Progo River, Magelang, Central Java. The method used in this study was the PCR method with universal primers 16Sar and 16Sbr. Intraspecific genetic variation was conducted by combining mitochondrial gene 16S sequence data of five Asian redtail fish (H. nemurus) samples from Progo River (BGM 02-06) and five Asian redtail fish samples from GenBank. BLAST, DnaSP, DNASTAR, GeneStudio,, MEGA, Mesquite, and NETWORK programs were used to analyze the data obtained. The results showed there were no genetic differences among the Asian redtail fish from Progo River indicating no intrapopulation genetic variation of the fish species. Conversely, the intraspecific genetic divergence was between 0.20% and 2.21% with an average of 0.92%. Analysis of intraspecific genetic variations detected 5 different haplotypes and 15 variable sites with 5 parsimony informative sites. Haplotype diversity and nucleotide diversity values were 0.756 ± 0.130 and 0.00812 ± 0.00294 respectively. Analysis of phylogenetic and haplotype network revealed BGM 02-06 formed a specific haplotype that was separated from other haplotypes. Molecular markers for Asian redtail fish (H.nemurus) from the Progo River, Magelang, Central Java based on the 16S mitochondrial gene can use this specific haplotype.

PENGARUH RENTANG WAKTU PENINGKATAN SALINITAS TERHADAP KELULUSHIDUPAN DAN PERTUMBUHAN LARVA IKAN BAUNG (H. nemurus)

This research is expected to be useful especially for fish farmers as additional information in raising baung fish. The method used was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 3 replications namely: P1 = 5 ‰ per 3 days 7 ‰ per 3 days, 9 ‰ per 3 days, P2 = 5 ‰ per 5 days 7 ‰ per 5 days 9 ‰ per 5 days, P3 = 5 ‰ per 7 days 7 ‰ per 7 days 9 ‰ per 7 days, P4 = 5 ‰ per 9 days 7 ‰ per 9 days 9 ‰ per 9 days. The baung fish used for the study was obtained from spawning at the Fish Seed Center (BBI) of the Faculty of Agriculture, Riau Islamic University with a weight of 0.14 gr of baung fish/tail and 1.6 cm in length/tail. The container used is a jar a size of 10 liters. From the results of the study obtained the best absolute weight growth and absolute length of the treatment (P4) with a weight of 4.35 gr and a length of 1.84 cm, followed by treatment (P2) with a weight of 3.96 gr and P3 a length of 1.82 cm, (P3 ) weighing 3, 75 gr and P2, 1.73 cm long, and the lowest in treatment (P1) with a weight of 2.64 grams and a length of 1.28 cm. The daily weight growth rate in the treatment group (P2) was 17.31%, (P2) 15.17%, (P4) 13.18%, and the lowest (P3) was 12.60%. The best survival rate for treatment (P4) was 72.00%, followed by treatment (P3) at 54.67%, and the lowest for treatment (P1) was 6, 67%. The results of water quality measurements in this study were temperature 28-300C, pH 6-7, dissolved oxygen 0.4 ppm, ammonia 0.05 ppm. Based on the observations of water quality during the study was within the range of tolerance for the growth and life of baung fish.

Training on Baung Fish (Mytus Nemurus) Hatchery Techniques for Fish Cultivators in Palembang City, South Sumatra Province

Catfish (Mytus nemurus) is a local fish with high commercial value and used as a food raw material for the traditional food in South Sumatra called pindang. The training aimed to enhance the Fish Farmer Group (Pokdakan) skills in Palembang City, which is part of community service at the Muhammadiyah University of Palembang. The method is divided into two, i.e., presented material and demonstrations, followed by a training program on Mytus nemurus breeding. The training was to produce catfish larvae, increase Pokdakan skills to produce catfish fingerling that improves the economic welfare of aquaculture actors, and increase Pokdakan's awareness of sustainable local fish production.

Optimization of Probiotic Addition in Feed on Growth of Baung Fish (Hemibagrus nemurus)

Baung fish is one type of local fish that has a slow growth. One of the efforts to increase the growth of baung fish can be done by using probiotics. This study aimed to determine the optimum dose of probiotic addition for the baung fish rearing. The experiment used a completely randomized design (CRD) with four treatments, three replications, and one control. The probiotic doses: 2 ml/kg of feed (A), 4 ml/kg of feed (B), 6 ml/kg of feed (C), 8 ml/kg of feed (D) and 0 ml/kg of feed (control) were used as treatment. The commercial feed was given ad satiation with a frequency of feeding three times a day. Observations were made for two months. The results showed that the D treatment of 8 ml/kg of feed gave the best absolute weight growth and feed conversion ratio. While, the baung fish survival rate was not significantly different on all treatments.Keywords: Baung fish, growth, probiotics

Optimization of Probiotic Addition in Feed on Growth of Baung Fish (Hemibagrus nemurus)

Baung fish is one type of local fish that has a slow growth. One of the efforts to increase the growth of baung fish can be done by using probiotics. This study aimed to determine the optimum dose of probiotic addition for the baung fish rearing. The experiment used a completely randomized design (CRD) with four treatments, three replications, and one control. The probiotic doses: 2 ml/kg of feed (A), 4 ml/kg of feed (B), 6 ml/kg of feed (C), 8 ml/kg of feed (D) and 0 ml/kg of feed (control) were used as treatment. The commercial feed was given ad satiation with a frequency of feeding three times a day. Observations were made for two months. The results showed that the D treatment of 8 ml/kg of feed gave the best absolute weight growth and feed conversion ratio. While, the baung fish survival rate was not significantly different on all treatments.Keywords: Baung fish, growth, probiotics

The Effect of Differences of Live Feed on The Growth And Survival of Redtail Catfish (Hemibagrus Nemurus) Larvae.

Baung fish is one of the local fish in West Kalimantan which has high economic value. One of the problems that arise in the maintenance of baung fish larvae is the low survival and growth rate, the most strategic solution is to determine the right type of feed according to the feeding habits of the baung fish larvae. The purpose of this study was to obtain the type of natural food that was most effective in rearing the larvae of fish. The research design used a completely randomized design (CRD) with 4 treatments and 3 repetitions. Treatment types of natural feed used in this study were Artemia (A), Water Fleas (B), Silkworms (C) and Pellet Flour (D), and arranged randomly. The results of the research through analysis of variance (Anova) show that all treatments can have a significant effect on the absolute length growth rate variable and the survival rate variable. Based on Tukey's further test, the treatment that had the best effect on the growth rate of absolute length was treatment C, namely 9.20 ± cm, while the treatment with the highest effect on survival rate was treatment A which was 98.8Keywords: Natural Feed, Baung Fish, Growth, Survival

The Effect of Differences of Live Feed on The Growth And Survival of Redtail Catfish (Hemibagrus Nemurus) Larvae.

Baung fish is one of the local fish in West Kalimantan which has high economic value. One of the problems that arise in the maintenance of baung fish larvae is the low survival and growth rate, the most strategic solution is to determine the right type of feed according to the feeding habits of the baung fish larvae. The purpose of this study was to obtain the type of natural food that was most effective in rearing the larvae of fish. The research design used a completely randomized design (CRD) with 4 treatments and 3 repetitions. Treatment types of natural feed used in this study were Artemia (A), Water Fleas (B), Silkworms (C) and Pellet Flour (D), and arranged randomly. The results of the research through analysis of variance (Anova) show that all treatments can have a significant effect on the absolute length growth rate variable and the survival rate variable. Based on Tukey's further test, the treatment that had the best effect on the growth rate of absolute length was treatment C, namely 9.20 ± cm, while the treatment with the highest effect on survival rate was treatment A which was 98.8Keywords: Natural Feed, Baung Fish, Growth, Survival

Characteristics of Chemical Quality Reduction in Fresh Baung Fish (Mystus nemurus) with Different Methods of Death at Room Temperature

Baung fish is a fishery commodity that contains high water content so it is easy to decompose (high perisable product). The purpose of this study was (1) to examine the freshness level of the baung fish, (2) pH level, Total Volatile Base (TVB), and glycogen of the baung fish by different mortality death. This research was conducted using an experimental method, consisting of two steps: (1) preparation and observation of freshness level of fresh baungfish, (2) analysis of chemical reduction of fresh baungfish including pH, TVB, and glycogen content. The treatments of the research were (1) the baungfish died by itself (A1), (2) the fish were stabbed out the medulla oblongata (A2). Chemical reduction of fresh baung fish at room temperature was measured every 4 hours for 12 hours with parameters such as pH, TVB, and glycogen content. The data were analysed descriptively. The results of this research showed that the characteristics of fresh baung fish were move actively, healthy, difficult to catch, the opening and closing movements of the operculum were normal, and normal skin clour (not pale). The results of the chemical reduction test revealed that pH were 6.5 ± 0.05 at 0 hours to 7.0 ± 0.20 at 12 hours for treatment A1, while for treatment A2 was 6.3 ± 0.1 at 0 hours to 6, 6 ± 0.1 at 12 hours. The TVB test results showed 0.14 ± 0.04 at 0 hours to 31.32 ± 0.09 at 12 hours for A1 treatment. Whereas A2 treatment was 0.10 ± 0.03 at 0 hours to 26.30 ± 1.87 at 12 hours. Furthermore, glycogen levels showed at 6.67% ± 0.03 at 0 hours to 0.35% ± 0.13 at 12 hours for A1 treatment. While the A2 treatment was 7.69% ± 0.12 at 0 hours to 1.15% ± 0.26 at 12 hours.

Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene.

Background and Aim:Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene.Materials and Methods:In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences were analyzed using MEGA X to determine genetic variation and phylogenetic relationships.Results:In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08.Conclusion:Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS, BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.

Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene

Abstract. Budiariati V, Susmiati T, Waroh S, Putri RCA, Widayanti R. 2021. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas 22: 593-600. Catfish is one of the most demanding fish in Indonesia and served in a variety of traditional culinary. Due to their identical morphology and close relation between species in the order of Siluriformes, it is quite tricky to distinguish the species. This can be a threat to develop catfish production in Indonesia since there is a wide variety of catfish species in this mega biodiversity country. The study aimed to analyze the genetic diversity of Indonesian indigenous catfish especially those known as Baung fish by local people based on COII gene. The study also aimed to determine the phylogenetic relationship between the samples and compare them with the GenBank data. A total of 24 samples used in this study from 8 different rivers from 3 different islands and two samples were collected from coastal areas. The study results showed that there is genetic diversity of the Indonesian indigenous catfish based on COII gene. The sequences among 24 samples showed that from 691 nucleotides of COII gene, there were very subtle nucleotides differences of samples that originated from Bojonegoro, Magelang, and samples collected from Baru Beach, Yogyakarta. Based on COII amino acid sequences, 6 polymorphic amino acid sites were on-site number 64, 115, 123, 129, 144, and 165. The samples encoded LLB1 and LPB1 from Baru Beach, Yogyakarta, showed highest different amino acids in the six sites. Samples from the river of Central Java, Sumatra, and Kalimantan belonged to Bagridae family and consist of two different species Hemibagrus sp. and Mystus sp while samples from East Java belonged to Pangasiidae family. The Samples from coastal belonged to Ariidae family.