Bauhinia Acuminata Research Articles

Corrosion Inhibition Potential of Aqueous Extract of Bauhinia acuminata Linn Leaves on Carbon Steel in Hydrochloric Acid Medium

The corrosion of carbon steel in 1 N HCl was analyzed using an aqueous Bauhinia acuminata Linn (BaL) leaf extract. Weight loss method was used to determine the inhibitor efficaciousness and corrosion rate. When dissolved in water, a linneus plant leaf extract exhibit an inhibitory effect which is proportionate to the amount of inhibitors present. It is possible to speed up the corrosion process by increasing the inhibitors’ concentration. Studies have shown that the BaL leaves extract has a 97.30% inhibitory efficiency. By increasing the concentration of inhibitor solutions, a surface layer forms on carbon steel, which inhibits the activity of the active site. The potentiodynamic polarization (PDP) and AC impedance studies (EIS) were employed to examine the mechanical properties of corrosion inhibition. The surface homogeneity and irregularity of carbon steel were examined using AFM and SEM under polished, corroded and inhibitor-treated surfaces. The surface irregularity of the carbon steel were analyzed using atomic force microscopy.

Analysis and Characterization of Four Selected Sri Lankan Seed Oils for the Potential Applications in Cosmetics and Dietary Supplements

As a tropical country, Sri Lanka has a wide variety of oil-bearing seeds explicitly used in Ayurvedic and indigenous medicine. Plant seed oils offer an exciting avenue for the use in cosmetic and dietary supplements due to their fatty acid composition and other non-polar constituents specially found in unsaponifiable fraction. This study aimed to characterize plant seed oils by determining Fatty Acids (FA) composition, constituents in unsaponifiable matter, and other physical properties. Mesua ferrea L. (Na), Crotalaria juncea L. (Hana), Ricinus communis L. (Thel edaru), and Bauhinia acuminata L. (Koboleela) seeds having some medicinal values were selected. The oil was extracted using soxhlet extraction method with Hexane. Moisture and ash content, Acid Value (AV), Iodine value (IV), and Smoke Point were also determined. Prepared Fatty Acid Methyl Esters (FAME) and unsaponifiable matter were analyzed using GC-MS. Thermogravimetric Analysis (TGA) was performed to access the thermal stability. M. ferrea obtained the highest oil yield of 63.87% while others lay within the range of 28.59-29.94%. Smoke point values of these oils varied from 179.0±10.5oC to 241.5±6.2o C. AVs lay between 4.62±1.18-49.64±2.92 mgKOH/g range. IVs ranged from 6.82±0.89 to 16.82±0.45 gI2/100g. Ash content values varied from 1.42±0.33% to 3.69±0.29% while the moisture content ranged from 0.69±0.09% to 4.46±0.51%. The yield of unsaponifable matter of these four seed oils ranged from 0.7024±0.0001% to 1.5864±0.0001%. All except M. ferrea oil exhibited higher levels of unsaturated fatty acids than saturated fatty acids. In contrary M. ferrea contained almost equal amounts of UFAs and SFAs. The most abundant FA constituents of all the oils were long-chain FAs like Oleic, Linoleic, and α-Linolenic Acid. γ- Sitosterol was the most dominant compound in unsaponifiable fraction in most of the seed oils which indicate their potential use in dietary supplements. The high level of Squalene found in R. communis shows its applicability in the cosmetic industry as a moisturizing agent. This analysis found that all 4 oils have the potential to use in dietary supplements and cosmetics. 
 Keywords: Seed oils, Unsaponifiable matter, Fatty acids, Cosmetics and dietary supplements

Phyto-Pharmacological Investigation of Ethanolic Extract of Flowers of Bauhinia acuminata

Bauhinia acuminata is a type of angiosperm in the Fabaceae family, native to South and Southeast Asia, South China, Burma, India, Nepal, Pakistan, and Sri Lanka. The common names include B. acuminata, mountain ebony, camel’s foot tree, kachnar, and butterfly ash. Angiosperm tree could be widely used as a medicinal plant common in tropical regions. Flowers, buds, stems, roots, bark, seeds, and leaves have been used to treat many ailments since ancient times. NSAIDs are one of the most important classes of drugs used today, and several clinical problems require long-term use. As a result of long-term use, side effects, especially stomach ulcers, can worsen the patient’s clinical symptoms. So there is a requirement to go for painkillers, but it is not related to problems even with chronic use. The literature says that a lot of analytical work has been done on this plant, but none of it has been evaluated for its analgesic and anti-inflammatory effects. An aqueous ethanolic extract was prepared and its analgesic, anti-inflammatory, and antipyretic effects were evaluated in animal models. The results showed that the flower extract at 200 mg/kg dose had significant analgesic, anti-inflammatory, and antipyretic effects compared to the medicinal active drugs.

Development, Evaluation and in vitro Release Kinetics Study of Immediate Release Tablets of Quercetin Isolated from Bauhinia acuminata Leaves Extract

Objective: To isolate quercetin, a flavonoid, from leaves extract of Bauhinia acuminata and to formulate and evaluate immediate release tablets of this quercetin employing direct compression method. Methods: Quercetin was isolated by column chromatography and characterized by FTIR, 1H and 13C NMR. Next different formulation blends were prepared using the isolated quercetin with microcrystalline cellulose, magnesium stearate and sodium starch glycolate. Pre-compression studies were performed and tablets were prepared by direct compression method. Tablets were evaluated for various post formulation parameters including disintegration and in vitro dissolution. Release kinetics and release mechanism were also studied from different kinetics plots. Finding : The pre-compression parameters were within the range of hardness (3.37  0.058 to 3.78  0.058 kg/cm2), weight variation (251.25  5.38 to 251.45  5.66 mg), friability (<1%), Drug content (98.17  0.86 to 98.20  0.99 %), disintegration (187.17  2.48 to 126.5  2.26 sec) and in vitro dissolution study (85.33  0.95 % and 86.47  0.70 %) within 30 minutes and 96.84 % and 98.19 % ) within 1 hr respectively for F2 and F3. Novelty: Only few scientific reports are present for Bauhinia acuminata and majority of them are related to pharmacological properties of the extract from the plant only. Here in this study quercetin was isolated for the very first time from Bauhinia acuminata to the best of our knowledge and not only this, the isolated quercetin was formulated to immediate release tablet which is again being reported from this plant for the first time. Keywords: Bauhinia acuminata; Immediate Release Tablet; Quercetin; Super Disintegrating Agents; Release Kinetics

Development of a validated high-performance thin-layer chromatography method for quantification of lupeol from different parts of Bauhinia acuminata.

The goal of the study was to determine how much lupeol was present in different Bauhinia acuminata samples. Lupeol was analyzed using a unique (verified) high-performance thin-layer chromatography (HPTLC) method. Separation of various compounds was achieved on silica gel (66 F 254) plates with toluene and methanol (99:1) as the mobile phase, followed by derivatization with Liebermann-Burchard reagent (r2 = 0.996). The proposed approach demonstrated a satisfactory linear relationship in the concentration range of 4-8 μg/mL. The limit of detection and limit of quantification were 63.84 ng/spot and 208.57 ng/spot, respectively. The proposed method was a simple and precise procedure that provided good resolution with other constituents of extracts. The validated HPTLC method described was simple, dependable, and practical and could be useful for standardizing and evaluating the quality of herbal material and formulations containing various Bauhinia species. The leaf, stem, unsaponified matter leaf, and stem petroleum ether extract had good amounts of lupeol (25.75, 38.25, 1.65, and 1.75, respectively). This research will be helpful for the quantitative determination of lupeol from herbal formulations as well as useful for further studies in this area.

Restoring Forest With High Density Plantation Method in Medchal District in Telangana State

A study was conducted to find the performance of tree species under Miyawaki method of planting in Chengicherla Range Forest and Rachakonda Commssionarate Range Forest in Medchal District of Telangana State. The plantation was taken up during 2016. Thirty fast growing trees namely Tectona grandis, Pterocarpus santalinus, Bambusa vulgaris, Bombax ceiba, Peltophorum pterocar, Delonix regiapum and medium canopy species are Grevillea robusta, Phyllanthus emblica, Pongamia pinnata, Delonix regia, Tecoma stans, Bauhinia acuminata, Punica granatum, Limonia acidissima and Low canopy Species. Ocimum tenuiflorum, Acalypha hispida, Lawsonia inermi, Caesalpinia pulcherrima, Bauhinia variegata, Murraya koenigii, Bougainvillea glabra, Gliricidia sepium respectively are found highly suitable for afforestation in wastelands and also for urban forest. The unexpected natural disasters such as flood, drought, rise in sea level, could affect not only the community but ecology of the region also. The change in climate has adverse effects on food chains, plant, and animal habitats. For addressing climate variations, restoring forest ecosystems and recreating forests could be better solution. Because historical deforestation and degradation of natural resources resulted in a high density of wild plant species and the construction of forests might take many years, the concept of creating green cover in a shorter period of time is gaining traction across continents.

MOLECULAR DOCKING STRATEGY FOR MULTI-TARGET INHIBITOR DISCOVERY OF SELECTED PLANT CONSTITUENTS IN BAUHINIA ACUMINATA

Objective: Traditional medicine is often considered to be a kind of complementary or alternative medicine (CAM) nowadays. Therefore, documenting and identifying the herbs that are effective in treating various diseases is vital for future disease control programs. This study aims to perform a molecular docking analysis of the thirteen plant components in Bauhinia acuminata against the target proteins in lung cancer (PDB IDs: 2ITY), breast cancer (1A52), diabetes (3L4U), obesity (IT02), inflammation (5COX) and corona viral infections (6VYO). Material and Method: All the plant components used for the present study were retrieved from the plant Bauhinia acuminata and were evaluated for their biological activity results using molinspiration. Further in-silico docking analysis was performed using AutoDock Vina software and the binding interactions were visualized using Discovery studio program. Result and Discussion: The docking scores and analysis of the interactions of the plant components with targets suggest that all the selected plant components showed excellent binding to the chosen targets when compared to that of the standard drugs. As a result of the docking process on 6 different targets, the selected plant components like Quercetin, Beta-sitosterol, and Rheagenine were observed to show good binding energy values against all the 5 targets except 6VYO as shown in (Table 9). These results can further pave the way for getting better insights in identifying and designing potential lead candidates.

Study of Phenotypic Variability of Kachnar (Bauhinia spp.)

An investigation to Study of phenotypic variability of Kachnar (Bauhinia spp.) was conducted in the Department of Floriculture and Landscape Architecture, IGKV Raipur, Chhattisgarh, during the Rabi season of 2021–2022. Five species for the study were Bauhinia purpurea, Bauhinia acuminata, Bauhinia blakeana, Bauhinia tomentosa and Bauhinia variegata. Different growth characteristics including plant, leaf, flower, and fruit traits, were recorded. According to the findings, Bauhinia purpurea had the tallest plants followed by Bauhinia blakeana, Bauhinia variegata, Bauhinia acuminata, and Bauhinia tomentosa. The longest and widest leaf was measured in Bauhinia purpurea 14.91and 12.04 respectively. Smallest and narrowest leaf was recorded in Bauhinia tomentosa 4.71 and 4.20 respectively. Among all the five species of Bauhinias highly pubescent leaf was discovered in Bauhinia tomentosa only. The Bauhinia acuminata's 3.96 long petiole had the record for being the longest while the Bauhinia tomentosa's 2.10 petiole had the smallest. The Bauhinia blakeana flower measuring 13.160.07 cm had the largest flowerdiameter while Bauhinia tomentosa 3.91 0.41 cm was the smallest. The only Bauhinia species with a pubescent flower bud was Bauhinia tomentosa. Longest petal, stamen and pistil length recorded in Bauhinia blakeana measuring 7.09, 4.8 and 6.91 respectively. Maximum pod length measuring 29.33 and maximum number of seeds per pod 11.90 was recorded in Bauhinia purpurea and minimum pod length measuring 9.25 in Bauhinia acuminata. Minimum number of seeds per pod recorded in Bauhinia acuminata. Bauhinia blakeana did not produce any pods as these species were infertile. The maximum seed weight was recorded in Bauhinia purpurea 30±0.87g and minimum in Bauhinia tomentosa 3.35±0.44g.

FLOWER PHENOLOGY AND FLORAL MORPHOLOGY OF BAUHINIA ACUMINATA L. GROWING AT GORAKHPUR DISTRICT OF EASTERN UTTAR PRADESH

FLOWER PHENOLOGY AND FLORAL MORPHOLOGY OF BAUHINIA ACUMINATA L. GROWING AT GORAKHPUR DISTRICT OF EASTERN UTTAR PRADESH

Bauhinia acuminata L. attenuates lung cancer cell proliferation: in vitro, in vivo and in silico approaches

BackgroundThe present study aims to explore the efficacy and suitability of Bauhinia acuminata L. to attenuate lung cancer on in vitro lung cancer cell lines, in vivo benzo(a)pyrene (BaP) induced mice model and in silico molecular docking. MethodsBoth in vitro and in vivo models were used to assess the efficacy of chloroform crude extracts of B. acuminata (CEBA) leaves fractions. For in vitro cytotoxicity analysis, A549 and Vero cancer cell lines were used. The BaP treated and untreated C57BL/6 mice were grouped and the in vivo acute toxicity levels were analyzed. Ethyl acetate (EA) fraction of CEBA was orally administrated in BaP treated mice and various parameters were measured in serum, liver, lung and kidney tissues. In addition, the histological, enzymatic, immunohistochemical, p53 expression and in silico molecular docking was carried out. ResultsCEBA was found to be cytotoxic against lung cancer cell lines. The oral administration of CEBA revealed good anti-lung cancer potential in BaP induced lung cancer mice. The enzymatic and non-enzymatic antioxidants level significantly increased after the treatment of CEBA. In addition, the biochemical and histological parameters were normal in CEBA treated animals. The molecular docking showed good binding between ligands of ethyl acetate fraction and the receptors of lung. ConclusionThis study confirmed the anti-proliferative capability of ethyl acetate fraction of chloroform extract of B. acuminata on lung cancer models. These findings may represent a promising candidate for cancer therapy and used for further investigations in the clinical studies.

MOLECULAR PROPERTIES, BIOACTIVITY SCORES, AND TOXICITY PREDICTIONS OF THE PHYTOCONSTITUENTS PRESENT IN BAUHINIA ACUMINATA

ABSTRACT:&#x0D; To develop the herbal drug with the least side effects, there are superior opportunities to discover the medicinal and other biological properties. Natural products serve as sources of beneficial chemical molecules. For this study, Bauhinia acuminata an important medicinal plant of the Indian subcontinent that belongs to the family Fabaceae was chosen. The plant is well known for its precautionary action in tuberculosis. It has been established to possess some pharmacological activities such as membranes Stabilizing activity1, antibacterial2, anti-nociceptive3, thrombolytic activity4, antioxidant5, anthelmintic6, anti-diarrheal7, Hepato-protective 8.&#x0D; Phytoconstituents present in Bauhinia acuminata obey Lipinski's rule (MiLog P &lt;5) except Kaempferol-3-glucoside indicated their drug-likeness property. Rhoeagenine, 9, 12, 15-octadecatrienoic acid, and 9, 12-octadecadienoic acid are the phytoconstituents showing all types of binding with all types of receptors binding except Kinase inhibitor activity.&#x0D; Rhoeagenine, Alpha humulene, 9, 12, 15-octadecatrienoic acid, 9, 12-octadecadienoic acid, Alpha muurolol, Beta-sitosterol, Kaempferol-3-glucoside are the phytoconstituents that are free from any type of toxicity. The accurate prediction scores can be used as monographs by researchers and scientists for the development of potential Semisynthetic and synthetic drugs for multifarious usage.

Bauhinia acuminata: A brief review of its phytochemistry and pharmacology

Bauhinia acuminata: A brief review of its phytochemistry and pharmacology

ASOSIASI TALI KUPU-KUPU (Bauhinia acuminata Blume.) DENGAN POHON INANG PADA PLOT MONITORING TAMAN WISATA ALAM GUNUNG MEJA MANOKWARI

Gunung Meja Nature tourist Park is one of the low land tropical forest ecosystems in Manokwari which had various potention of its flora and fauna. The flora potention had relation or complex population, one of them is association between tali kupu-kupu (Bauhinia acuminata Blume.) with its host tree. The purpose of the study was to describe the asociation of B. acuminata with its host tree and its habitat at the low land at Gunung Meja Nature Tourist Park. The method used description with survey technique. The resluts showed that the total population of the trees associating with B. acuminate. There were 10 species i.e. Pometia coreacea, Pometia pinnata, Alstonia scholaris, Artocarpus incissus, Pimelodendron amboinicum, Pterocymbium beccarii, Antiaris toxicaria, Streblus elongate, Medusanthera laxiflora, and Pterygota horsfieldii which dominated by Pometia pinnata 11 trees. The association relation level of B. acuminata with the host tree P. pinnata was very close (2.506 %) while association relation level of B. acuminata was quite close with the host tree of A. scholaris (0.911 %), P. amboinicum (0.911 %), and the association level B. acuminata was not close with the host tree P. coreacea (0.683 %), P. beccarii (0.228 %), A. toxicaria (0.228 %), A. incisus (0.683 %), P. horsfieldii (0.683 %), S. elongata (0.456 %), and M. laxiflora (0.456 %).

Exploration of the Mechanism of Action of the Anti-Inflammatory Potential of the Stem Bark of Bauhinia Acuminata LINN. Throguh In-Vivo Experiments and Lc-Ms/Ms.

Context and objective: Bauhinia acuminata Linn.is used in inflammatory diseases by the local traditional healers of Purulia district of West Bengal, India. In the present study, an attempt has been made to dissect out the probable mechanism of action of the anti-inflammatory profile of Bauhinia acuminata stem bark by using LC-MS/MS along with various in vivo experimental models. Material and method: The hydro-alcoholic extract of the stem-bark of Bauhinia acuminata Linn.(HEBA) was subjected to preliminary phytochemical analysis and evaluated for acute toxicity study, carrageenan induced rat paw edema (analysis of PGE2 was done by LC-MS/MS) and acetic acid-induced peritoneal inflammation. Evaluation of analgesic activity was performed in models like radiant heat induced algesia (tail flick method) and acetic acid induced writhing. Results and conclusions: The extract showed significant inhibition in increase in paw volume of the right hind paw as compared to control group in carrageenan-induced rat paw edema. In acetic acid-induced peritoneal inflammation, pre-treatment with the extract demonstrated significant inhibition of acetic acid induced peritoneal protein exudation and also reduction in peritoneal leukocytes count. In the tail flick test, HEBA hardly produced any significant effect but it has shown significant inhibition against acetic acid induced writhing in mice. The effect might be attributed to the combined effect of phytoconstituents like flavonoids and glycosides and others present in the extract. In conclusion, an optimized, highly sensitive and specific LC-MS/MS technique for the detection of main arachidonic acid metabolites formed in the rat paw tissue in the late phase of the carrageenan induced inflammation model is presented here along with the other in-vivo models. Overall, these results undoubtedly confirm the anti-inflammatory activity of the hydro-alcoholic extract of the stem bark of the Bauhinia acuminata Linn., along with an indication of its mechanism of action.

MICROSPORE ANALYSIS FOR GENOTOXICITY OF POLLUTED ATMOSPHERIC ENVIRONMENT (THE CASE STUDY IN THE MATARAM CITY)

Abstract : The need for environmental protection continues to increase, so exploratory studies of agents of polluted environmental indicators are becoming increasingly important. The advantages of using flowering plants for environmental bio-indicator studies include the availability of sufficient test material with their variations and the availability of different periods for sampling. Seven plant species were used to examine their suitability as bio-indicator for genotoxicity of polluted atmospheric environment in Mataram City by microspore analysis. This study was conducted with the aim of, 1) knowing the level of difference in the frequency of failure (abortiveness) of microspores between similar plants that grow in areas at risk of being polluted in the city and those growing in areas not at risk of being polluted outside the city of Mataram, and 2) find out what types of flowering plants, especially those in city parks that are suitable as bio-indicators of air pollution for Mataram City. The percentage of aborted pollens was calculated out of 50 to 300 observed pollen grains of each selected individual flowering plant. Sample of each plant species from every study area consist of ten plant individuals. Data were analyzed by use the Wilcoxon one way-test at a 0.05 to determine significance of difference in the percentage of aborted pollen grains from same plant species between in the risky polluted area and plant in the control area. The results showed that Lantana camara L. is suitable as bio-indicator for atmospheric pollution in Mataram City or other cities that have a density of traffic flow of motorized vehicles around 100 vehicles per hour. Its percentage of aborted pollen grains is 1,62 ± 0,08 % in the risky polluted area, and  1,21 ± 0,13 % in the control area. Others six observed plant spesies, i.e. Acacia auriculiformis A. Conn. Ex Bth., Bauhinia acuminata L., Baugenvillea spectabilis Willd. Duranta erecta L., Ixora coccinea L. and Rosa hybryda Hort., each of them is not suitable as bioindicator for it. Their percentage of pollen abortiveness in polluted and not-polluted areas were not different, significantly.Keywords: Genotoxicity, Microspores, Polluted air environment

Total phenolic content and antioxidant activity of methanolic extract of selected wild leafy vegetables grown in Bangladesh: A cheapest source of antioxidants.

Nowadays, more attention has been paid on wild plants as new source of natural antioxidants. Therefore, methanolic extracts of 10 traditionally consumed wild leafy vegetables of Bangladesh were analyzed for their total phenolic content (TPC) and free radical scavenging activity. Folin-Ciocalteu method followed by spectrophotometric measurement was used to quantify the TPC of the selected wild leafy vegetables. Free radical scavenging activity was examined utilizing 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. Different concentrations of the plant extract were applied to ascertain the dose response relationship in inhibiting DPPH free radical. The results revealed that the TPC ranged from 102.20 to 710.42 mg GAE/100g dry weight (DW). The highest TPC was observed in Bauhinia acuminata (Shetokanchan) while Leucas aspera (Shetodhron) exhibited the lowest TPC among the undertaken vegetables. The studied samples proportionately inhibited DPPH with increasing concentrations. At high concentration (500 µg.mL-1), the percentage inhibition of DPPH radical by plant extract ranged from 68.1 ±2.65 % to 93.1 ±1.23 %. The highest DPPH radical inhibition was observed in Bauhinia acuminata (Shetokanchan) (93.10 ±1.23 %), followed by Commelina benghalensis (Bat baittashak) (91.97 ±1.31 %), Hydrocotyle sibthorpiodes L. (Sakumubakla) (91.83 ±2.13 %). The lowest DPPH radical inhibition among the studied samples was observed in Leucas aspera (Shetodhron) (68.1 ±2.65 %). IC50 values measured by DPPH assay in this study ranged from 11.64 to 313.79 µg.mL-1. The study findings indicated that the samples under study possesses strong activity against DPPH, and thus could be used as natural antioxidants in the food and/or pharmaceutical industry.

Nanosilver crystals capped with Bauhinia acuminata phytochemicals as new antimicrobials and mosquito larvicides

To develop novel nanoformulated insecticides and antimicrobials, herein we produced Ag nanoparticles (AgNPs) using the Bauhinia acuminata leaf extract. This unexpensive aqueous extract acted as a capping and reducing agent for the formation of AgNPs. We characterized B. acuminata-synthesized AgNPs by UV–vis and FTIR spectroscopy, XRD and TEM analyses. UV–vis spectroscopy analysis of B. acuminata-synthesized AgNPs showed a peak at 441.5 nm. FTIR shed light on functional groups from the phytoconstituents involved in nanosynthesis. XRD of B. acuminata-synthesized AgNPs suggested a face-centered cubic structure, with a highly crystalline nature. TEM of B. acuminata-synthesized AgNPs revealed mean size of 25 nm, with round shape. AgNPs tested at 60 μg/mL inhibited the growth of 5 bacteria and 3 fungal pathogens. In the insecticidal assays on important mosquito species, LC50 of the aqueous extract of B. acuminata leaves on the larvae of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus were 204.07, 226.02, and 249.24 μg/mL, respectively. The B. acuminata-synthesized AgNPs exhibited higher larvicidal efficacy, with LC50 values of 24.59, 27.19, and 30.19 μg/mL, respectively. Therefore, herein we developed a single-step, reliable, inexpensive, and environmentally non-toxic synthesis process to obtain AgNPs with high bioactivity against pathogens and vectors. Given the effective antimicrobial and larvicidal activity, nanoparticles fabricated using plant extracts and extremely low concentrations of trace elements, such as silver, can be exploited for multipurpose activities. Our results pointed out that B. acuminata-synthesized AgNPs have a promising potential in antimicrobial food packaging, as well as a foliar spray to control plant pathogens in the field, and to synergize the efficacy of fungicidal and larvicidal formulations.

Acute toxicity study and anti-nociceptive activity of Bauhinia acuminata Linn. leaf extracts in experimental animal models

Bauhinia acuminata commonly known as dwarf white orchid tree is traditionally used to treat acute and chronic pain, skin ailments, cancer, diabetes, throat infections and asthma. As there were no scientific reports on use of Bauhinia acuminata for anti-nociceptive activity, the present study was designed to evaluate possible effects of aqueous and alcoholic extracts in experimentally induced pain in animals. Acute toxicity was carried out as per OECD guideline 423. The anti-nociceptive activity was evaluated in Swiss albino mice by hot plate, acetic acid induced writhing and tail immersion tests at three different dose levels (250, 500 and 1000mg/kg) of aqueous and alcoholic extracts. Formalin induced nociception test was performed in Sprague Dawley rats at three dose levels. Both aqueous and alcoholic extracts were found safe at dose of 5000mg/kg. In hot plate test, both extracts showed significant (p<0.001) anti-nociceptive activity. In acetic acid writhing test, both aqueous and alcoholic extracts significantly reduced number of writhes (p<0.001). In Tail immersion test, both the extracts showed significant increase in tail withdrawal response (p<0.001). Treatment with aqueous and alcoholic extracts significantly reduced nociception in formalin induced nociception model (p<0.001). From the results it can be concluded that aqueous and alcoholic extracts possesses potent anti-nociceptive activity.

Antidiabetic Potential of Methanolic Extract of Leave and Bark of Bangladeshi Medicinal Plant Bauhinia acuminata L on Mice

Diabetes mellitus the one of four priority non-communicable diseases is an important public health issue across the world. The disease is associated with abnormalities in Carbohydrate, Protein and Fat metabolism affecting 422 million adults globally (WHO). Though many antidiabetic agents have been introduced in the modern drug arsenal in the recent past, still diabetes and the related complications continue to be alarming health problem in developed as well as in developing countries. Hopefully, there are many plants has been reported to have potent antidiabetic activity and have been used for a long time in traditional medicine. However, the screening for potent antidiabetic molecule from natural sources is still going on. The antidiabetic activity was evaluated in normal and Streptozotocin (STZ) induced diabetic mice. Remarkable lowering of blood glucose levels (90.19% by leaf extract and 76.86% by bark extract compared to positive control)of the test animals depicts that the methanolic leaf and bark extract exhibited significant property (P<0.05) compared to diabetic control group. Dose dependent effect was also reflected in the test results. Control animals were found to be stable in their body weight while diabetic mice showed significant reduction in body weight during 7 days. The antidiabetic activity produced by a single mechanism or multiple glucose lowering mechanism may be involved.

A Comparative Study of Plant Mediated Synthesis of Silver Nanoparticles from Fresh Leaf Extracts of Gracina Gummi-Gutta L., Cynodon Dactylon L. and Bauhinia Acuminata and Their Antimicrobial Activity Studies

A Comparative Study of Plant Mediated Synthesis of Silver Nanoparticles from Fresh Leaf Extracts of Gracina Gummi-Gutta L., Cynodon Dactylon L. and Bauhinia Acuminata and Their Antimicrobial Activity Studies