Bathyal Research Articles

CD169+ subcapsular sinus macrophage-derived microvesicles are associated with light zone follicular dendritic cells.

Follicular dendritic cells (FDCs) are a specialized type of stromal cells that exclusively reside in B-cell follicles. When inflammation occurs, the FDC network is reorganized to support germinal center (GC) polarization into the light zone (LZ) and dark zone (DZ). Despite the indispensable role of FDCs in supporting humoral responses, the FDC regulatory requirements remain incompletely defined. In this study, we unexpectedly observed an accumulation of CD169+ subcapsular sinus macrophage (SSM)-derived microvesicles (MVs) in the B-cell zone, which were tightly associated with the FDC network. Interestingly, a selective deposition of CD169+ MVs was detected in both GC LZ FDCs in secondary follicles and on predetermined LZ FDCs in primary follicles. The ablation of CD169+ MVs, resulting from SSM depletion, resulted in significantly decreased expression of LZ-related genes in FDCs. In addition, we found that CD169+ MVs could colocalize with fluorescently tagged antigen-containing immune complexes (ICs), supporting a possible role of CD169+ MVs in transporting antigens to the FDC network. Thus, our data reveal intimate crosstalk between FDCs and SSMs located outside B-cell follicles via SSM-released MVs, providing a novel perspective on the mechanisms underlying the regulation of FDC maturation and polarization.

Simple and efficient realization of transverse linear dark beams by azimuthal phase-shifted square zone plate

Herein, we are about to introduce a novel, reliable and straightforward method to create controllable dark lines employing an azimuthal square zone plate. As a matter of fact, this diffractive element is a square zone plate whose zones are phase-shifted azimuthally. As we illustrate, one way to construct it is combining a square zone plate and radial grating having period m. Considering its focusing behavior, we came to the result that a dark line surrounded by linear bright zones is generated for odd m, as well as a cross-like dark zone is produced when m is even. Furthermore, we illustrate that the length of the dark lines depends on the grating period. Finally, the simulation predictions are verified by experimental results.

Clonal germinal center B cells function as a niche for T-cell lymphoma

AbstractAngioimmunoblastic T-cell lymphoma (AITL) is proposed to be initiated by age-related clonal hematopoiesis (ACH) with TET2 mutations, whereas the G17V RHOA mutation in immature cells with TET2 mutations promotes the development of T follicular helper (TFH)-like tumor cells. Here, we investigated the mechanism by which TET2-mutant immune cells enable AITL development using mouse models and human samples. Among the 2 mouse models, mice lacking Tet2 in all the blood cells (Mx-Cre × Tet2flox/flox × G17V RHOA transgenic mice) spontaneously developed AITL for approximately up to a year, while mice lacking Tet2 only in the T cells (Cd4-Cre × Tet2flox/flox × G17V RHOA transgenic mice) did not. Therefore, Tet2-deficient immune cells function as a niche for AITL development. Single-cell RNA-sequencing (scRNA-seq) of >50 000 cells from mouse and human AITL samples revealed significant expansion of aberrant B cells, exhibiting properties of activating light zone (LZ)-like and proliferative dark zone (DZ)-like germinal center B (GCB) cells. The GCB cells in AITL clonally evolved with recurrent mutations in genes related to core histones. In silico network analysis using scRNA-seq data identified Cd40–Cd40lg as a possible mediator of GCB and tumor cell cluster interactions. Treatment of AITL model mice with anti-Cd40lg inhibitory antibody prolonged survival. The genes expressed in aberrantly expanded GCB cells in murine tumors were also broadly expressed in the B-lineage cells of TET2-mutant human AITL. Therefore, ACH-derived GCB cells could undergo independent clonal evolution and support the tumorigenesis in AITL via the CD40–CD40LG axis.

3D Sparse ISAR Imaging With Multiple Plane Spiral OAM Electromagnetic Waves

Vortex electromagnetic (EM) waves carrying orbital angular momentum (OAM) have a helical phase front and a field strength with a singularity along the axial center, which is expected to obtain resolutions beyond the Rayleigh limit for imaging systems, and provide new instruments for accurate radar targets imaging. However, since the vortex EM beams feature a doughnut-like intensity profile with a central dark zone and the inconsistent divergence angles of the main lobe, it is difficult to simultaneously illuminate the target for vortex EM beams with different OAM mode, which results in limited echo energy. In addition, the restricted elevation resolution hinders the acquisition of target spatial information, which limits the development of the OAM-based radar detection technique. This contribution offers a new set of ideas and solutions for achieving the 3D ISAR imaging using the superposition of different plane spiral OAM (PSOAM) waves. First, eigenmode beam steering techniques based on radio PSOAM waves is introduced. Subsequently, the PSOAM-based ISAR imaging model for maneuvering targets is derived and the echo characteristics are analyzed. Third, the effective imaging method, based on the amplitude phase compensation and fast Tensor-SL0 reconstruction algorithm, is proposed to solve the 3D sparse imaging problem in a tensor way. Finally, simulations are carried out to verify that the proposed method not only significantly reduces the computational complexity of the image reconstruction, but also generates the 3D image with sidelobes and artifacts removed remarkably from sparse measurements.

Distributions and relationships of virio- and picoplankton in the epi-, meso- and bathypelagic zones of the Amundsen Sea, West Antarctica during the austral summer.

Virioplankton and picoplankton are the most abundant marine biological entities on earth and mediate biogeochemical cycles in the Southern Ocean. However, understanding of their distribution and relationships with environmental factors is lacking. Here, we report on their distribution and relationships with environmental factors at 48 stations from 112.5° to 150°W and 67° to 75.5°S in the Amundsen Sea of West Antarctica. The epipelagic stations were grouped into four clusters based on the virio- and picoplankton composition and abundance. Clusters three and four, which were associated with the ice-edge blooms in the coastal and Amundsen Sea Polynya (ASP) areas, had high abundances of autotrophic picoeukaryotes; this resulted in subsequent high abundances of heterotrophic prokaryotes and viruses. Cluster two stations were in open oceanic areas, where the abundances of autotrophic and heterotrophic picoplankton were low. Cluster one stations were located between the areas of blooms and the oceanic areas, which had a low abundance of heterotrophic prokaryotes and picoeukaryotes and a high abundance of virioplankton. The abundance of viruses was significantly correlated with the abundances of autotrophic picoeukaryotes and Chl-a concentration in oceanic areas, although this reflected a time-lag with autotrophic picoeukaryote and heterotrophic prokaryotes abundances in ice-edge bloom areas. The upwelling of Circumpolar Deep Water (CDW) might have induced the high abundance of autotrophic picoeukaryotes in the epipelagic zone, and the sinking particulate organic carbon (POC) might have induced the high abundance of heterotrophic prokaryotes and virioplankton in the meso- and bathypelagic zones. This study shows that the summer distribution of virio- and picoplankton in the Amundsen Sea of West Antarctica was mainly controlled by upwelling of the CDW and the timing of ice-edge blooms.

Application of Ultrasound Combined with Magnetic Resonance Imaging in the Diagnosis and Grading of Patients with Prenatal Placenta Accreta.

In order to study the clinical application value of placenta accreta (PIA) diagnosis and grading, the authors propose a method based on ultrasound combined with magnetic resonance imaging in the diagnosis and grading of prenatal placenta accreta patients. This method is adopted in materials and methods: a retrospective analysis of hospital patients with high suspicion of placenta accreta by clinical or ultrasonography between October 2019 and October 2021, the imaging and clinical data of 312 patients who underwent placental MRI examination. The MRI imaging data of all patients were jointly analyzed, and the main observation indicators are as follows: (1) dark zone in the placenta, (2) disruption of the border of the myometrium, (3) disruption of the myometrium, (4) abnormal blood vessels in the placenta, (5) enlargement of the lower part of the uterus, and (6) local bulge of the bladder/or invasion of the adjacent tissues of the uterus. The results show the following: in MRI combined with ultrasonography (P < 0.05), there was no statistical significance in the specificity and accuracy of MRI combined with ultrasound to diagnose PIA (P > 0.05). The comparison of graded diagnostic accuracy showed that in ultrasound alone < MRI alone < MRI combined with ultrasound, the differences were statistically significant (P < 0.05). Ultrasound combined with MRI in the diagnosis of placenta accreta is in good agreement with the clinical and surgical pathological results; MRI examination can be used as an important method for prenatal placenta accreta screening. MRI can classify placenta accreta to some extent.

Two Sound Field Control Methods Based on Particle Velocity

In recent years, a variety of sound field control methods have been proposed for the generation of separated sound regions. Different algorithms control the physical properties of the generated sound field to different degrees. The existing methods mainly focus on sound pressure restoration and its related improvement. When the loudspeaker array is non-uniformly placed, the reconstruction system is not stable enough. To solve this problem, this paper proposes two sound field control methods related to particle velocity. The first method regulates the reconstruction error of particle velocity in the bright zone and the square of particle velocity in the dark zone; the second method regulates the reconstruction error of sound pressure and particle velocity in the bright zone and the square of sound pressure and particle velocity in the dark zone. Five channel and twenty-two channel non-uniform loudspeaker systems were used for two-dimensional and three-dimensional computer simulation testing. Experimental results show that the two proposed methods have better tradeoffs in terms of acoustic contrast, reproduction error and array effort than traditional methods, especially the second proposed method. In the two-dimensional experiment, the maximum reductions of the average array efforts generated by the proposed methods were about 10 dB and 11 dB compared with the average array efforts generated by two traditional methods. In the three-dimensional experiment, the maximum reductions of the average array efforts generated by the proposed methods were about 8 dB and 2 dB compared with the average array efforts generated by two traditional methods. The smaller the array effort, the more stable the loudspeaker system. Therefore, the reconstruction systems produced by the proposed methods are more stable than those produced by the traditional methods.

Evaluation of Alfalfa and Grass Species in Binary and Complex Mixtures on Performance under Soil Salinity Conditions

The effect of synergies between saline-tolerant grass and legume species grown in saline soil, on yield, quality, composition, persistence, and weed suppression was studied in 2019–2021 in a moderately saline soil located in the dark brown soil zone near Saskatoon, Saskatchewan, Canada. ‘Halo’ alfalfa (ALF), a salt-tolerant cultivar of alfalfa, was seeded in binary mixtures with ‘Revenue’ slender wheatgrass (ALF-SWG), ‘Garrison’ creeping meadow foxtail (ALF-CMF), and ‘Radisson’ smooth bromegrass (ALF-SBG). A complex mixture (quaternary) with all three grasses was also included. Four replicated treatments (n = 4) were randomly allocated to 6.2 × 1.2 m plots in spring 2019. The binary mixtures had similar or slightly better stand establishment compared to the quaternary mixture over the two years (83.3 vs. 76.9%), the quaternary stand showed greater (p = 0.01) establishment in Yr 2 than in Yr 1 (85 vs. 68.8%). There was high weed infestation in all the stands, but ALF-SWG had less weed infestation (23.8 vs. 44.1%) than ALF-CMF, especially in Yr 2 (p = 0.05). There were no significant differences among mixtures in yield, nutritional composition, and nutrient uptake. The ALF-CMF and ALF-SWG binary mixtures had 9 to 23% greater mean total DMY than the other mixtures in the moderately saline soil. The cost of establishing forage mixtures on unproductive saline land can be up to 89% recovered after only two years. The results suggested that binary mixtures of ‘Halo’ alfalfa with ‘Revenue’ slender wheatgrass or ‘Garrison’ creeping meadow foxtail could be reasonable alternatives for adequate forage production and quality, high N-use efficiency, and ultimately livestock gain per hectare, as well as for controlling soil salinity and improving soil fertility in this saline area in the dark brown soil zone.

Soil Chemical Properties, Metabolome, and Metabarcoding Give the New Insights into the Soil Transforming Process of Fairy Ring Fungi Leucocalocybe mongolica.

A unique ecological landscape distributed in the Mongolian Plateau, called fairy rings, caused by the growth of the fungus Leucocalocybe mongolica (LM) in the soil could promote plant growth without fertilization. Therefore, this landscape can alleviate fertilizer use and has excellent value for agricultural production. The previous studies only investigated several parameters of the fairy rings, such as soil microbial diversity and some soil chemical properties, thus conclusions based on the studies on fairy rings lack comprehension. Therefore, the present study systematically investigated the chemical properties, metabolome, and metabarcoding of LM-transformed soil. We analyzed fairy ring soils from DARK (FR) and OUT (CK) zone correlated growth promotion with ten soil chemical properties, including N, nitrate-N, inorganic-P, cellulose, available boron, available sulfur, Fe, Mn, Zn, and Cu, which were identified as important markers to screen fairy ring landscapes. Metabolomics showed that the accumulation of 17 carbohydrate-dominated metabolites was closely associated with plant growth promotion. Finally, metabarcoding detected fungi as the main components affecting soil conversion. Among the various fungi at the family level, Lasiosphaeriaceae, unidentified_Auriculariales_sp, and Herpotrichiellaceae were markers to screen fairy ring. Our study is novel and systematically reveals the fairy ring soil ecology and lists the key factors promoting plant growth. These findings lay a theoretical foundation for developing the fairy ring landscape in an agricultural system.

Aberrant expansion of spontaneous splenic germinal centers induced by hallmark genetic lesions of aggressive lymphoma

AbstractUnique molecular vulnerabilities have been identified in the aggressive MCD/C5 genetic subclass of diffuse large B-cell lymphoma (DLBCL). However, the premalignant cell-of-origin exhibiting MCD-like dependencies remains elusive. In this study, we examined animals carrying up to 4 hallmark genetic lesions found in MCD consisting of gain-of-function mutations in Myd88 and Cd79b, loss of Prdm1, and overexpression of BCL2. We discovered that expression of combinations of these alleles in vivo promoted a cell-intrinsic accumulation of B cells in spontaneous splenic germinal centers (GCs). As with MCD, these premalignant B cells were enriched for B-cell receptors (BCRs) with evidence of self-reactivity, displayed a de novo dependence on Tlr9, and were more sensitive to inhibition of Bruton's tyrosine kinase. Mutant spontaneous splenic GC B cells (GCB) showed increased proliferation and IRF4 expression. Mice carrying all 4 genetic lesions showed a >50-fold expansion of spontaneous splenic GCs exhibiting aberrant histologic features with a dark zone immunophenotype and went on to develop DLBCL in the spleen with age. Thus, by combining multiple hallmark genetic alterations associated with MCD, our study identifies aberrant spontaneous splenic GCBs as a likely cell-of-origin for this aggressive genetic subtype of lymphoma.

Классическая и неклассическая эпистемология пещер (часть 2)

In the second part of the article, the authors “collect” the non-classical model of epistemology of caves, determining its paradigmatic specificity and characterizing the analytical approach that is fundamental to it. It is argued that the origins of this type of epistemology of caves can be found in antiquity as well, namely, in book 13 of Homer’s Odyssey and in the exegesis On the Cave of the Nymphs by Porphyry of Tyre, dedicated to a fragment in this book. In contrast to the classical, the non-classical model is defined as a glance-into-the-Beyond. From the authors’ point of view, the sensual Homeric cave turns the “darkness of the mystery” into the basic symbol of the non-classical epistemology of caves. Mystery is understood as the unrevealed or the unresolved, that is, something that transforms darkness into the “charming darkness”, i.e. into the synergy of the visible and the intelligible. As a result of the cognizer’s transitions into the depths of the “dark zone” of the cave, the latter loses its status as the Beyond, since darkness ceases to be the limit of cognition. Thus, the apophatic approach inevitably becomes the fundamental approach of the non-classical model. It is associated with the cognitive sciences, such as glaciospeleology and astrobiology. The authors step-by-step reveal the anthropological and spelestological dimensions of the non-classical epistemology of caves, focusing on the dominant – speleological and (more broadly) geological – dimension, which expands to the cosmic dimension. Caves and other cavities inside the Earth’s crust, primarily under the ocean floor and glacial layers, are considered not just as keepers of the mystery of the origin of life on Earth, but as models of such keepers on other planets of the Solar System and analogues of molecular clouds. It is emphasized that, due to the fixation on the search for the first principle of the nature of all matter, the non-classical model is rather close to early Greek philosophy, e.g. to the theories of Heraclitus of Ephesus. A conclusion for the two parts of the article is formulated: the anthropological dimension, which is key to both the classical and non-classical epistemology of caves, indicates the dynamics of redefining the role of the cave in human life.

The Growth Zones of Test Plates in Four Spatangoid Echinoid Species: Irregular Pattern.

Whether the growth zone found in the coronal plate of echinoids can be used as a chronometer to estimate the age of individuals is controversial. In spatangoid echinoids, it is not known whether the growth zone formation occurs annually. In this study, the growth zone formation process was examined in four spatangoid species from natural conditions. In Nacospatangus alta and Brissus agassizii, the formation of a dark zone indicates the maturity of the gonad and does not necessarily correspond to the age of the individual. In Lovenia elongata, dark growth zones were formed several times per year. Unlike in the other studied species, in Metalia spatagus, the light and dark growth zones appeared to be formed radially, rather than in a ring-like manner. These results may indicate that the frequency and mode of growth zone formation differ across species. As such, it is not appropriate to use the growth zone as a chronometer in spatangoid echinoids. If the growth zone is to be used as an indicator of age, more research is needed about the phase of forming the growth zone in each echinoid species.

Evaluation of New Fall Rye Cultivar ‘Bono’ in Single and Double Cropping Systems

A new fall rye (FR, Secale cereale L.) cv. Bono was investigated as a novel cropping option in Saskatchewan, Canada. In this study, the performance of Bono was compared to Hazlet FR, and both cultivars were compared to winter triticale (WT, Triticosecale Wittm.) cv. Pika in single cropping (SC) or in double cropping (DC) systems with spring barley (Hordeum vulgare L.) was evaluated in the Dark Brown soil zone, 2019–2021. Five replicated (n = 4) treatments were: (i) BonoFR; (ii) HazletFR; (iii) PikaWT; (iv) Barley–BonoFR; and (v) Barley–HazletFR. The first crop of barley was harvested at soft dough stage, followed by the second crop of FR seeded in the same year and harvested between flag leaf to heading emergence the following summer for greenfeed hay. Bono did not differ (p &gt; 0.05) in DMY (1.2 Mg ha−1) or nutritive value from Hazlet, however, both FRs differed (p = 0.01) from WT by higher nitrogen use efficiency (NUE, 41.0 vs. 33.7) and NDF (541.8 vs. 479.3 g kg–1), but lower CP (155.3 vs. 187.1 g kg–1). Double cropping barley with fall ryes increased total DMY, nutrients yield per ha, and minerals uptake by up to 83% and NUE by 35.3%. In conclusion, Bono fall rye could be an equal quality alternative to Hazlet, although the current higher seed price may delay its adoption.

Toxicological assessment of cadmium-containing quantum dots in developing zebrafish: Physiological performance and neurobehavioral responses.

The present research investigated the effects of exposure to sublethal concentrations of cadmium selenide/zinc sulfide (CdSecore/ZnSshell)-containing quantum dots (QDs; 0 - 100µg/L QDs) on the neurophysiological performance of developing zebrafish (Danio rerio). The results suggested that exposure to CdSe QDs for 5 days increased the whole-body content of Cd without affecting the general physiological conditions of larvae. Interestingly, CdSe QD exposure reduced swimming distance but increased swimming velocity of larvae, suggesting that the exposure may lead to burst/episodic swimming. The findings also suggested that CdSe QD exposure reduced the wall-hugging behavior of larvae during a sudden light-to-dark transition test, and that the exposure significantly decreased the locomotor activity of fish during the dark period. On the other hand, control larvae displayed a dark avoidance behavior, whereas CdSe QD-exposed larvae exhibited an increase in the time spent in the dark zone, providing further support that CdSe QDs inhibited anxiety-related responses in larvae. Additional analysis with droplet digital PCR revealed that CdSe QD exposure altered the mRNA levels of genes that are associated with dopamine signaling and oxidative stress response. Collectively, our findings suggested that CdSe QD exposure may induce neurobehavioural toxicity and alters the mRNA abundance of dopamine- and oxidative stress-related genes in developing animals.

Sustained Anxiety-Like Behavior in Crayfish Exposed to Thermal Burn

Both invertebrates and vertebrates make immediate simple reactions to noxious stimuli; a tail flip to escape is a typical example in crayfish. More complex sustained emotional responses, such as anxiety or fear, can be demonstrated in many vertebrates but until recently have not been seen in crustaceans. Several recent studies have found sustained anxiety-like behavior (ALB) in crayfish in response to electric shock and social bullying, and this behavior can be suppressed by anti-anxiety medications. In this study, crayfish were subjected to a thermal burn stress, and then observed for ALB in an established model (light-dark maze). Stressed animals spent almost all of their time in the dark zone, in contrast to unstressed crayfish, who demonstrated normal crayfish exploratory behavior. In this model, crayfish clearly demonstrated ALB in response to stressful burn. This research has implications for humane treatment of crustaceans.&#x0D;

Mapping the habitats of a complex circalittoral rocky shelf in the Cantabrian Sea (south Bay of Biscay)

This work focuses on the study of habitats and communities of a high structural complexity area at different levels and scales. This gives us a better understanding of an area from an ecological point of view and at the same time provides us with tools that will facilitate management measures. It was developed in a complex circalittoral rocky platform of the central Cantabrian Sea (Bay of Biscay). The sampling was carried out using a towed photogrammetric vehicle and a rock dredge, which was used for the identification of the species. The first level of the study was the abiotic characterization of the area and the analysis of the communities. These analysis were developed using the unsupervised k-means classification method. For abiotic characterization we used the variables directly associated with the composition and morphology of the ground, such as backscatter, BPI (Bathymetric Position Index), roughness and slope. Depth was also included to discriminate between the circalittoral and bathyal zones. We obtained 5 different classes, which we related to the ground types observed by photogrammetry. In the analysis of the communities, the cluster was based on the sampling units extracted from the images (∼10 m), from which 5 assemblages were obtained, providing information on the most abundant species of each class supplied by the abiotic study. The second level was carried out considering a management approach and was based on the modeling of the area at lower resolution, more suitable for the analysis of the habitat-fisheries interactions. Thus, the main habitat-forming species (HFS) of the entire circalittoral area were used to perform delta models based on GAMs (Generalize Additive Models). Obtaining the predictions of presence/absence and combining it with the predictions of densities, we got the zero inflated values density-based model. As all the identified habitats have vulnerable benthic species of a certain size settled on rocky bottoms, they can all be considered to belong to the designation 1170 reefs of the Habitats Directive.

Diversity and distribution of bivalve molluscs in the Central Cantabrian Sea and the Avilés Canyons System (Bay of Biscay)

Bivalve molluscs is one of the dominant benthic groups in soft-sediment communities off the Atlantic Ocean, including the European margin and the Bay of Biscay. The current knowledge of deep-sea Euro-Atlantic bivalves and their distribution is still far from comprehensive and has been the scope of several recent publications. We examined the collected bivalve samples from two oceanographic campaigns (COCACE and BIOCANT) carried out in the central Bay of Biscay, including the Avilés Canyons System (ACS), a Site of Community Importance within the Natura 2000 Network. Bivalve specimens were collected from the continental shelf, slope and bathyal zones, ranging in deep from 25 to 4700 m. This is the first study focused on bivalve diversity from the Cantabrian Sea that covers such a wide bathymetric range. Eighty-four live taxa in 36 families were identified; Cetomya neaeroides is reported for the first time in the area. We provide photographs showing the shell diagnostic features of all recorded species. The conducted Multivariate Analysis showed three species assemblages: i) samples from shallow waters (continental shelf); ii) intermediate depths (shelf and upper slope) and iii) deep water (slope and abyssal plain). Depth was the main structuring factor that influenced the distribution of the recorded species and the higher species richness was found on the continental shelf. The feeding strategy and the substrate preference appeared as not significant factors in the zonation patterns of the studied bivalves.

Rehabilitation of two deep-sea holothurian species in genus Pannychia from the northwest Pacific Ocean

Genus Pannychia Théel, 1882 is one of the dominant sea cucumber genus in the bathyal zone of the North Pacific Ocean. Previously seven species/subspecies were described in Pannychia and another closest genus Laetmophasma Ludwig, 1894 from all over the Pacific Ocean, but all of them were synonymized into Pannychia moseleyi Théel, 1882, which is currently single valid species in the Pacific Ocean. In this study, we rehabilitate Pannychia henrici Ludwig, 1894 and Pannychia virgulifera Ohshima, 1915 as separate species from the northwest Pacific Ocean, which were originally established as geographical variation/subspecies of P. moseleyi. This study includes molecular phylogenetic analysis based on specimens collected from broad part of the northwest Pacific Ocean, and morphological comparison including re-examination of both subspecies type specimens. Genetically these two northwest Pacific taxa and P. moseleyi are clearly distinguished from each other, which corresponded to different characteristics of dorsal papillae arrangement, the body coloration, and the ossicle density in dorsal body wall. As a result of rehabilitation of P. henrici and P. virgulifera, the genus Pannychia was reorganized to 4 valid species, but P. moseleyi still include large morphological variations, and it is necessary to a further taxonomic revision. Additionally, P. henrici and P. virgulifera particularly represent an example of importance of body coloration in taxonomy of the deep-sea Holothuroidea.

Microanalysis of the stomach of southern white-breasted hedgehog (Erinaceus concolor): Histological, histochemical, immunohistochemical, and scanning electron microscopic studies.

This study was designed to provide more detailed knowledge on the stomach histochemistry and immunohistochemistry in the southern white-breasted hedgehog (Erinaceus concolor). Two animals were used in the present work. Periodic acid Schiff's (PAS) and Alcian blue were used for histochemical purposes. SOX9, gastrin, serotonin, and glucagon markers were traced immunohistochemically. The mucosa was extremely folded in the fundus with numerous opening of glands. The body and pylorus mucosa were almost smooth and equipped with gastric gland openings. A simple columnar epithelium covered the stomach entirely. Cardiac glands region was mucus secreting with both positive and negative reactions to PAS. Fundic mucosa was contained cardiac glands near to the cardia, and toward the body it was divided into the light and dark zones. These zones and body contained proper gastric gland, which constituted of parietal, chief, and mucous neck cells. These glands contained PAS-positive cells on their basal portions. The pyloric glands were mucus secreting but negative for PAS. All gastric glands were Alcian blue-negative, but epithelium showed moderate reaction especially in the pylorus. SOX and gastrin were express highly in the body and fundus. The expression of serotonin and glucagon was rare. Comparatively, some similarities between the stomach of hedgehog and dog can be assumed. The present findings provide additional information concerning the histochemical characteristics and endocrine cells distribution in the stomach of the southern white-breasted hedgehog (Erinaceus concolor). Further detailed studies are required to enhance the current knowledge on histophysiology of the digestive system in this species as a pet and exotic animal. HIGHLIGHTS: The stomach was simple glandular type. The fundus was divided into light and dark zones similar to the dog. The proper gastric glands were periodic acid Schiff's positive at their basal parts.

IL-4 Signaling Regulates the Fate of B Cell Differentiation and limits BCR repertoire

Abstract Il-4 has a critical role in organizing the peripheral lymph nodes in homeostasis and after vaccination. To further understand the molecular mechanisms and their impact in the immune response, we used single cell RNA-Seq of CD45+ cells in 4get and 4getIL4R−/− mice following tetanus/diphtheria immunization. We found that the lack of IL-4 signaling restrains the expression of key co-stimulatory and differentiation molecules CD83, CD86, and xbp1, while upregulating the jun/junb/fos pathways. These alterations result in reduced B cell proliferation and differentiation into plasma cells. Surprisingly, we found that in the absence of IL-4 signaling the ratio of dark to light zone residing germinal center shifts from the normal ratio to one dominated by the dark zone. The increase in Dark Zone germinal centers lead to an increase in the diversity of the repertoire in vaccinated 4getIL4R−/− mice as confirmed by VDJ-seq. We also observed that the upregulation of the transcription factor T-bet has the capacity of inducing a similar phenotype. Finally, even though the amount of memory B cells was increased in the absence of IL-4 signaling, we observed that these cells are not able to respond to a secondary challenge. These data suggest a pivotal role for IL-4 in governing B-cell differentiation and the development of optimal cellular and humoral immunity to vaccination. supported by grants from NIH R01 AI135045