Blood Basophils Research Articles

Co-variates associated with outcomes of tyrosine kinase-inhibitor therapy in persons with chronic myeloid leukaemia initially presenting in accelerated phase

We interrogated data from 278 consecutive subjects with chronic myeloid leukaemia (CML) presenting in accelerated phase diagnosed by European LeukemiaNet(ELN) criteria receiving initial imatinib (n = 187) or a 2nd-generation tyrosine kinase-inhibitor (2G-TKI; n = 91). In multi-variable analyses, blood and/or bone marrow blasts ≥15% (Hazard ratio [HR] = 3.7 [1.6, 8.5], p = 0.003) and blood basophils <3% (HR = 4.6 [2.0, 10.7], p < 0.001) were significantly-associated with worse transformation-free survival (TFS). Age ≥60 years (HR = 4.3 [1.7, 11.4], p = 0.003), platelet concentration <230 × 10E + 9/L (HR = 4.7 [2.0, 10.7], p < 0.001) and blood and/or bone marrow blasts ≥9% (HR = 3.9 [1.7, 8.7], p = 0.001) were significantly-associated with worse survival. Based on number of adverse prognostic co-variates of TFS and survival, respectively, subjects were classified into the low- (none), intermediate- (one) and high-risk (≥2) cohorts with significant difference in TFS and survival (all p < 0.001). In propensity-score matching analysis subjects initially receiving a 2G-TKI had higher cumulative incidences of cytogenetic and molecular responses but similar TFS and survival to those receiving imatinib. Our data should help inform physicians treating person with CML initially presenting in accelerated phase.

Hematogenesis Adaptation to Long-Term Hypoxia Acclimation in Zebrafish (Danio rerio)

When fish live in the wild or are cultured artificially, they will inevitably suffer from hypoxia. At the same time, blood physiological indexes represent the physiological state of fish. In order to study the effect of long-term hypoxia acclimation on fish hematogenesis, we cultured zebrafish embryos into adulthood in a hypoxia incubator (1.5 ± 0.2 mg/L). Then we compared the hematological parameters of zebrafish cultured in normoxia and hypoxia conditions. Transcriptome sequencing analysis of the main hematopoietic tissue, the head kidney, was also compared between the two groups. Results showed that the number of erythrocytes increased significantly in the long-term hypoxia acclimated group, while the size of several cell types, such as red blood cells, eosinophils, basophils, small lymphocytes and thrombocytes, decreased significantly. The transcriptomic comparisons revealed that there were 6475 differentially expressed genes (DEGs) between the two groups. A Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that hematopoiesis and cell proliferation signaling were the most significantly enriched pathways in the head kidney of hypoxia acclimated zebrafish. In addition, many genes involved in the hematopoietic process showed significantly higher levels of expression in the hypoxia acclimated zebrafish, when compared to the normoxia zebrafish. When considered together, these data allowed us to conclude that long-term hypoxia can promote the hematopoiesis process and cell proliferation signaling in the zebrafish head kidney, which resulted in higher red blood cell production. Higher numbers of red blood cells allow for better adaptation to the hypoxic environment. In conclusion, this study provides a basis for the in-depth understanding of the effects of hypoxia on hematogenesis in fish species.

Clinical and Laboratory Features in the Israeli Population with COVID-19 Infection after Pfizer-BioNTech mRNA Booster Vaccination.

Background: Immune protection following either vaccination or infection with SARS-CoV-2 decreases over time. Objective: We aim to describe clinical and sociodemographic characteristics associated with COVID-19 infection at least 14 days after booster vaccination in the Israeli population. Methods: We conducted a population-based study among adult members of Leumit Health Services (LHS) in Israel. Nasopharyngeal swabs were examined for SARS-CoV-2 by real-time RT-PCR. The hematological and biochemical parameters in the peripheral blood before booster vaccination were evaluated. Results: Between 1 February 2021 and 30 November 2021, 136,683 individuals in LHS were vaccinated with a booster (third dose) of the BNT162b2 vaccine. Of these, 1171 (0.9%) were diagnosed with COVID-19 by testing positive for SARS-CoV-2 RT-PCR at least >14 days after the booster vaccination. The COVID-19-positive group was characterized by higher rates of chronic kidney disease than the matched COVID-19-negative group (43 (3.7%) vs. 3646 (2.7%); p = 0.039). Anemia, lower peripheral blood lymphocytes, monocytes, basophils, C3 Complement, cholesterol, and prothrombin time were also associated with COVID-19 after booster vaccination. Conclusion: People with chronic kidney disease and anemia should be included in possible future annual SARS-CoV-2 vaccination recommendations.

Risk factors for central venous catheter-associated deep venous thrombosis in pediatric critical care settings identified by fusion model

BackgroundAn increase in the incidence of central venous catheter (CVC)-related thrombosis (CRT) has been reported in pediatric intensive care patients over the past decade. Risk factors for the development of CRT are not well understood, especially in children. The study objective was to identify potential clinical risk factors associated with CRT with novel fusion machine learning models.MethodsPatients aged 0–18 who were admitted to intensive care units from December 2015 to December 2018 and underwent at least one CVC placement were included. Two fusion model approaches (stacking and blending) were used to build a better performance model based on three widely used machine learning models (logistic regression, random forest and gradient boosting decision tree). High-impact risk factors were identified based on their contribution in both fusion artificial intelligence models.ResultsA total of 478 factors of 3871 patients and 3927 lines were used to build fusion models, one of which achieved quite satisfactory performance (AUC = 0.82, recall = 0.85, accuracy = 0.65) in 5-fold cross validation. A total of 11 risk factors were identified based on their independent contributions to the two fusion models. Some risk factors, such as D-dimer, thrombin time, blood acid-base balance-related factors, dehydrating agents, lymphocytes and basophils were identified or confirmed to play an important role in CRT in children.ConclusionsThe fusion model, which achieves better performance in CRT prediction, can better understand the risk factors for CRT and provide potential biomarkers and measures for thromboprophylaxis in pediatric intensive care settings.

PSV-2 Beneficial Impact of Black Soldier Fly (Hermetia Illucens) Larvae Oil on Growth Performance of Nursery Pigs

Abstract Black soldier fly larvae (BSFL) oil is a sustainably sourced insect-derived lipid that is notably high in lauric acid. The objective of this study was to determine the effects of BSFL oil on nursery pig growth performance, and serological and hematological indices. This study was conducted using newly weaned nursery pigs (n = 143; BW = 6.70±0.09 kg) that were randomly assigned within BW blocks to 22 mixed sex pens (5 to 7 pigs/pen; 10 to 12 replicated per treatment). Dietary treatments consisted of a control diet with 4% poultry fat or a diet with 4% BSFL oil replacing poultry fat. Treatment diets were fed in 3 phases of 14 d each, for a total study period of 42 d. Pigs fed BSFL oil had greater ADG for Phase 1 (P &amp;lt; 0.001), 2 (P &amp;lt; 0.001), 3 (P = 0.036), and overall (P &amp;lt; 0.001; 484 vs. 409 g/d), resulting in a greater BW at the end of the study (P = 0.005). Feed intake was greater for pigs fed BSFL oil during Phase 2 (P = 0.007) and overall (P = 0.022; 710 vs. 626 g/d), and gain:feed was improved only during Phase 1 (P=0.004). Supplemental BSFL oil did not affect (P &amp;gt; 0.06) serum concentrations of total protein, albumin, globulin, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transpeptidase, urea N, creatinine, glucose, Ca, P, Mg, K, Na, triglycerides, amylase, lipase, cholesterol, or creatine phosphokinase, but decreased serum alanine aminotransferase (P = 0.023) and increased serum Cl concentrations (P = 0.046). White blood cells, red blood cells, hemoglobin, hematocrit, neutrophils, lymphocytes, monocytes, eosinophils, basophils, and platelet count were not affected (P &amp;gt;0.07) by BSFL oil. Blood analytical results were within normal expected ranges. Results indicate that BSFL oil effectively improved growth performance of nursery pigs without impacting serological and hematological parameters.

Lead exposure-induced changes in hematology and biomarkers of hepatic injury: protective role of TrévoTM supplement.

Lead exposure has been linked to health challenges involving multiple organ failure. More than fifty percent of lead present in the human body is accumulated in the liver causing hepatic injury. A major mechanism of lead toxicity is oxidative stress. Trévo™ is a nutritional supplement with numerous bioactive natural products with detoxifying and antioxidant properties. This study was designed to investigate the hepatoprotective effects of Trévo™ dietary supplements against lead-hepatotoxicity in male Wistar rats. Thirty-five healthy animals were divided into five groups of seven each as follows: Group I=control; II=15 mg/kg of lead acetate (PbA); III= 2 mL/kg of Trévo™ + PbA; IV= 5 mL/kg of Trévo™ + PbA;V=5 mL/kg of Trévo™. Animals were orally treated with Trévo™ for two days before co-administration with PbA intraperitoneally for 12 consecutive days. Animals were sacrificed 24 h after the last administration and blood were collected via cardiac puncture and processed for hematological parameters and assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (ALB). The liver was excised and processed for markers of oxidative stress and histopathological examination. Intraperitoneal administration of 15 mg/kg of PbA caused a significant increase in serum concentration of AST, ALT, while the concentration of ALB was significantly decreased (P<0.001). PbA caused a significant reduction in packed cell volume, hemoglobin while the total white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were increased. Oxidative stress was significantly pronounced in the liver of rats exposed to PbA as observed in the high concentration of malonedialdehyde, decreased concentration of glutathione, the activity of catalase, superoxide dismutase, and glutathione-S-transferase. Pretreatment with Trévo™ was able to significantly prevent the anemic, oxidative damage, and hepatic injury initiated by PbA. Histological examination also corroborated the biochemical results. In conclusion, the study reveals that Trévo™ is effective in attenuating PbA-induced hepatotoxicity in male Wistar rats.

Cord blood sphingolipids are associated with atopic dermatitis and wheeze in the first year of life.

Background:Allergen-sensitized pregnant mice have increased plasma levels of the lipids β-glucosylceramides (βGlcCers) that are transplacentally transferred to the fetus, increased subsets of proinflammatory dendritic cells in the fetal liver and pup lung, and increased allergen-induced offspring lung inflammation.Objective:Our aim was to determine whether these preclinical observations extend to a human association of βGlcCers with wheeze and allergic disease in the prospective Wisconsin Infant Study Cohort.Methods:We measured 74 lipids in cord blood plasma by using mass spectrometry detection of sphingolipids, eicosanoids, and docosinoids, as well as an ELISA for 13-hydroxyoctadecadienoic acid. Lipid profiles were determined by unbiased Uniform Manifold Approximation and Projection dimensional reduction machine learning. Lipid profiles and a proinflammatory lipid index were analyzed for association with maternal allergy and childhood outcomes of wheeze, atopic dermatitis, cord blood leukocytes, and total IgE level at age 1 year.Results:Uniform Manifold Approximation and Projection analysis of lipids defined 8 cluster-specific plasma lipid profiles. Cluster 6 had significantly lower levels of plasma βGlcCers and a higher frequency of cord blood plasmacytoid dendritic cells that mediate anti-inflammatory responses, which is consistent with an anti-inflammatory profile. For clusters and for each infant, a proinflammatory lipid index was calculated to reflect the sum of the proinflammatory lipids minus the anti-inflammatory lipids that were significantly different than in cluster 6. The cluster proinflammatory lipid index was associated with cord blood basophil frequency and with wheeze and atopic dermatitis in the first year of life. The infant inflammatory lipid index was associated with increased risk of wheeze in the first year of life.Conclusion:The cord blood proinflammatory lipid index is associated with early-life atopic dermatitis and wheezing.

Effects of anti-IL5 biological treatments on blood IgE levels in severe asthmatic patients: A real-life multicentre study (BIONIGE).

BackgroundMepolizumab and benralizumab are clinically effective biological treatments for severe eosinophilic asthmatic patients by hampering eosinophilic inflammation. The effects of these compound on the immunoglobulin (Ig)E T2 component are virtually unknown.ObjectivesTo evaluate the change in total IgE levels at 4 ± 2 months after initiation of the mepolizumab (primary outcome) or benralizumab. When available, the changes of blood inflammatory cell counts, lung function and asthma control test (ACT) were also assessed and correlated with changes in total IgE levels.MethodsObservational, retrospective, multicentre, cohort study. Severe eosinophilic atopic asthmatic patients treated with mepolizumab or benralizumab were included in the analysis.ResultsThree‐month treatment (on average) with mepolizumab (n = 104) or benralizumab (n = 82) resulted in significantly higher reduction of blood eosinophil and basophil levels in patients treated with benralizumab compared to mepolizumab. Mepolizumab did not significantly modified the levels of blood total IgE during the study period, whereas benralizumab significantly reduced (−35%, p < 0.001) total blood IgE levels. In patients treated with benralizumab the reduction of blood total Ig‐E levels correlated with the reduction of blood basophils (but not eosinophils) and weakly with the improvement of asthma control.ConclusionBenralizumab but not mepolizumab, treatment led to a significant reduction of circulating IgE level. The study provides different and specific mechanisms of action for anti‐IL5‐pathway treatments.

Hemoglobin Level and Total Differential Blood Leucocytes of Broiler Chicken Given Different Probiotics

The use of adding probiotics to drinking water is an alternative to reduce the accumulation of additional chemical feed residues in meat, which functions to maintain the balance of the microflora ecosystem in the digestive tract and provide enzymes that can digest crude fiber, protein, fat and detoxify toxic substances or metabolites. This study aimed to determine the hemoglobin level and total differential leukocyte blood of broiler chickens given different probiotics using 100 broiler strains of MB 202 platinum with 20 cages with a size of 80X80 cm. The research design used a completely randomized design (CRD) with 4 treatments and 5 replications consisting of 5 chickens for each replication. The results showed that blood hemoglobin levels had a significant effect (P&gt;0.05) and the administration of different probiotics into drinking water had no significant effect (P&gt;0.05) on the differential value of leukocytes (monocytes, eosinophils, heterophils, and basophils) in broiler blood, but giving different probiotics had a significant effect (P&lt;0.05) on blood lymphocyte levels in broiler chickens.

Performance, Serum Biochemical and Haematological Response of Broiler Chicken Fed Mixture of Ginger (Zingiber Officinale) and Garlic (Allium Sativum) in Diets

Performance, serum biochemical and hematological response of broiler chicken fed mixture of ginger (Zingiber officinale) and garlic (Allium sativum) in diets was investigated in a feeding trials using completely randomised design that lasted for eight weeks. A total of 135 1-day broiler chicks of Abor acre strain were randomly allotted to three dietary treatments of 45 chicks per treatment and replicated 15 chicks per treatment Three isocaloric and isonitrogenous diets were formulated and fed. Control diet was without mixture of ginger and garlic. The two other diets were T1, 7.5g Ginger plus 7.5g Garlic mixture/25kgdiet and T2, 15g Ginger plus 15g Garlic mixture/25kgdiet. The experimental diets were offered to the respective birds with water ad libitum. The body weight gain (Kg) feed intake and feed conversion ratio (FCR) of birds control, T1 and T2 respectively were similar (p&gt; 0.05). Birds on diet. T2 recorded the highest live ability percentage (96 Mixture of ginger and garlic in ratio 1:1 up to 7.5g each in 25kg diets slightly but insignificantly (p&gt;0,05) increased performance body weight gain and live ability improved feed conversion ratio (FRC) but reduced feed intake. Variation in the experimental diets had no significant (p&gt;0.05) difference on serum biochemical and hematological indices except for Glucose and Alkaline Phosphate, and White Blood Cell count Lymphocytes and Basophil which showed significant (p&lt;0.05) difference with the level mixture of ginger and garlic in diets. Mixture of ginger and garlic in the diets of broiler chicken had no adverse effect on the serum biochemical and hematological indices.

Haematological Parameters and Oxidative stress Changes in Apparently Healthy Pregnant Women in Pakistan

Pregnancy is associated with alterations in haematological and oxidative stress parameters as physiological adjustments are made to accommodate the increasing demand from the fetus and the maintenance of maternal wellbeing. In this cross-sectional study, baseline values for haematological and oxidative stress changes were evaluated in pregnant women attending an ante-natal clinic at selected private clinics in Lahore. A total of 100 subjects (80 pregnant women and 20 non-pregnant women) were recruited for the study. Haematological and oxidative stress parameters were determined following standard protocols. When comparing pregnant and non-pregnant women's white blood cell, neutrophil, monocytes, and catalase mean values (p 0.05), it was observed that the pregnant women's eosinophil mean values were lower than those of the non-pregnant control (p 0.05). During the third trimester, neutrophils and catalase levels were significantly higher in the pregnant group than they were in the non-pregnant group (p 0.05) Compared to catalase, superoxide dismutase was shown to be adversely associated with both mean cell volume and haemoglobin concentration. Other variables studied were mean haemoglobin, mean haemoglobin concentration, total white blood cell and neutrophil counts, and mean cell volume.s There was a negative correlation between glutathione and neutrophils and monocytes whereas a positive correlation was found between malondialdehyde and the mean cell haemoglobin content, total white blood cell, monocyte, eosinophil, and basophil count. According to the findings of this study, pregnant women's haematological parameters may be affected by oxidative stress. Keywords: Haematology, pregnancy, oxidative stress, antioxidant enzymes

Relationship between allergic transfusion reactions and allergic predisposition among pediatric patients with hematological/oncological disease.

Allergic transfusion reactions (ATRs) manifest frequently as transfusion reactions, and their onset may be related to a patient's allergic predisposition. Moreover, although pediatric patients with hematological/oncological disease are more susceptible to ATRs, the relationship between allergic predisposition and ATRs remains to be fully clarified. Patients who were diagnosed with pediatric hematological/oncological disease and received transfusion at the study institutions were included. We determined patient background information related to their allergy history, measured the levels of allergen-specific immunoglobulin E (IgE) using sera obtained on diagnosis, and analyzed their associations with ATR onset. Of the 363 patients analyzed, 144 developed ATRs. Multivariate analysis identified cases with high basophils in the peripheral blood, and Dermatophagoides pteronyssinus- and egg white-specific IgEs were involved in the development of ATR in all age groups. Meanwhile, a history of food allergies, and positivity for Japanese cypress- and D. pteronyssinus-specific IgEs were risk factors for developing ATRs in the <5 years age group. Moreover, patients aged 5-<10 years with a history of asthma, allergic rhinitis, pollinosis, or atopic dermatitis, and those aged ≥10 years with positivity for dog dander-specific IgE were at risk for developing ATRs. The allergic constitution of patients plays a role in ATR onset even in pediatric hematological/oncological diseases. Therefore, advance confirmation of a patient's allergic constitution may partly predict the onset of ATRs. However, since multiple allergic predispositions within complex mechanisms may be involved in the onset of ATRs, further verification is required.

Gut Microbiota-Related Inflammation Factors as a Potential Biomarker for Diagnosing Major Depressive Disorder.

ObjectiveAlthough many works have been done, the objectively measured diagnostic biomarkers are not available. Thus, we conducted this study to identify potential biomarkers for objectively diagnosing depression and explore the role of gut microbiota in the onset of depression.MethodsMajor depressive disorder (MDD) patients (n=56) and demographic data-matched healthy controls (HCs) (n=56) were included in this study. The gut microbiota in fecal samples and inflammation-related factors in serum were measured. Both univariate and multivariate statistical analyses were performed to identify the differential gut microbiota and inflammation-related factors.ResultsFinally, 46 differential operational taxonomic units (OTUs) (60.9% OTUs belonging to Firmicutes) and ten differential inflammation-related factors were identified. Correlation analysis showed that there were significant correlations between 14 differential OTUs (9 OTUs belonging to Firmicutes and 5 OTUs belonging to family Lachnospiraceae under Firmicutes) and seven differential inflammation-related factors. Meanwhile, 14 differential OTUs (9 OTUs belonging to Firmicutes and 5 OTUs belonging to family Lachnospiraceae under Firmicutes) and five differential inflammation-related factors (adiponectin, apolipoprotein A1, alpha 1-antitrypsin, neutrophilicgranulocyte count/white blood cell count and basophil count) were significantly correlated to depression severity. A panel consisting of these five differential inflammation-related factors could effectively diagnose MDD patients from HCs.ConclusionsOur results suggested that Firmicutes, especially family Lachnospiraceae, might play a role in the onset of depression via affecting the inflammation levels of host, and these five differential inflammation-related factors could be potential biomarkers for objectively diagnosing MDD.

Associations between solar and geomagnetic activity and peripheral white blood cells in the Normative Aging Study.

It has been hypothesized that solar and geomagnetic activity can affect the function of the autonomic nervous system (ANS) and melatonin secretion, both of which may influence immune response. We investigated the association between solar geomagnetic activity and white blood cell counts in the Normative Aging Study (NAS) Cohort between 2000 and 2013. Linear mixed effects models with moving day averages ranging from 0 to 28 days were used to evaluate the effects of solar activity measures, interplanetary magnetic field (IMF), and sunspot number (SSN), and a measure of geomagnetic activity, K Index (K), on total white blood cell (WBC), neutrophil, monocytes, lymphocyte, eosinophil, and basophil concentrations. After adjusting for demographic and health-related factors, there were consistently significant associations between IMF, SSN, and Kp index, with reductions in total WBC, neutrophils, and basophil counts. These associations were stronger with longer moving averages. The associations were similar after adjusting for ambient air particulate pollution and particle radioactivity. Our findings suggest that periods of increased solar and geomagnetic activity result in lower WBC, neutrophil, and basophil counts that may contribute to mil mild immune suppression.

A predictive scoring system for therapy-failure in persons with chronic myeloid leukemia receiving initial imatinib therapy.

Data from 1,364 consecutive subjects with chronic-phase chronic myeloid leukemia (CML) receiving initial imatinib-therapy were interrogated to identify co-variates predicting therapy failure. Subjects were randomly divided into training (n = 908) and validation datasets (n = 456). In the training dataset, WBC count ≥120 × 10E + 9/L, haemoglobin concentration <115 g/L, blood basophils ≥12% and European Treatment and Outcome Study for CML Long-Term Survival (ELTS) risk score were significantly-associated with failure-free survival (FFS). Each co-variate was assigned 1 point to develop the imatinib-therapy failure (IMTF) model except ELTS high-risk category which was assigned 2 points based on multi-variable regression coefficients. Area under receiver-operator characteristic curve values in the IMTF model for 1-, 3- and 5-year FFS were 0.79-0.84 in the training dataset and 0.78-0.85 in the validation dataset. Calibration plots showed high agreement between predicted and observed outcomes. Decision curve analyses indicated subjects benefited from clinical use of this model. Cumulative incidences of imatinib-therapy failure and probabilities of FFS among the 5 risk cohorts (very low-, low-, intermediate-, high- and very high-risk) using the IMTF model were significantly different (all p values < 0.001). The IMTF model also correlated with probabilities of progression-free survival and survival (all p values < 0.001). These data should help physicians optimize TKI-therapy strategy at diagnosis in persons with chronic phase CML.

IgE and high-affinity IgE receptor in chronic inducible urticaria, pathogenic, and management relevance.

BackgroundIgE and high‐affinity IgE receptor (FcεRI) expression on basophils have been scarcely explored in patients with chronic inducible urticaria (CIndU).ObjectivesTo investigate baseline serum IgE and FcεRI expression on blood basophils in a large cohort of CIndU patients and its relationship to treatment response.MethodsBaseline total serum IgE and basophil FcεRI expression measured by flow cytometry in 165 patients with CIndU was studied. The relationship of both parameters with the response to antihistamine and anti‐IgE (omalizumab) treatment was considered in a subsample of CIndU patients. FcεRI expression in basophils was assessed by mean fluorescence intensity (MFI) and basophil FcεRI standardized density (receptors/cell).ResultsThe median FcεRI expression standardized per density in blood basophils was found significantly higher in patients with CIndU compared to HCs. A positive correlation was found between IgE serum levels and basophil FcεRI expression. Basal FcεRI expression was not related to antihistamine treatment response. However, it was related to omalizumab, and patients responding to omalizumab showed higher basal basophil expression of FcεRI levels. Non‐responders to the antihistamine showed significantly higher IgE serum levels.ConclusionsFcεRI receptor overexpression in patients with CIndU shows almost the same pattern than chronic spontaneous urticaria. It seems to be independent of CIndU subtypes. Although additional studies would be welcome, our work highlights the relevance of FcεRI receptor regulation in CIndU supporting autoimmune basophil and mast cell activation and may be a biomarker for response to anti‐IgE therapy.

Complete Blood Count and Blood Chemistry of Samae Dam Chickens (Gallus gallus) in Small-Holder Farmers, Thailand

Complete blood count and blood chemistry of Samae Dam chickens (Gallus gallus) in smallholder farmers, Thailand as part of the study on production performance of native samae Dam chickens (Gallus gallus) of breeders in small-holder farmers. Complete blood count and blood chemistry were studied in thirty-six healthy small-holder farmers-Samae Dam chickens (Gallus gallus) (male, n = 18; female, n = 18), at the age of 8 months, which had been small-holder farmers at Uthai Thani Province in Thailand. The results found that red blood cells, hemoglobin, hematocrit, MCV, MCH, and MCHC were in the reference range, while male and female Samae Dam chickens (Gallus gallus) of small-holder farmers in Thailand had similar red blood cell and blood chemistry at age 0-3 months the difference was not statistically significant (P>0.05), and at the age of 3-6 months and 6-8 months, it was found that male hemoglobin values were higher than female hemoglobin values were significantly different (P<0.05). The average proportion at 8 months of age is as follows: Lymphocytes (43.26±0.44%), neutrophils (42.63±3.65%), white blood cells (35.63±3.65%), monocytes (1.26±0.44%), eosinophils (0.0%) and basophils (11.50±0.36%).

Relationship between Pre-Existing Conditions in Covid-19 Patients and Inflammation

COVID-19 is characterized by a strong inflammatory response leading to specific changes in the immune system cell counts. In this study, the following biomarkers from the laboratory data of COVID-19 patients have been analyzed: white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, C-reactive protein, and glycemia. Various comparisons, correlations, and ratios between these inflammatory biomarkers have been performed to assess the link between pre-existing conditio

Complete Blood Count Alterations of COVID‐19 Patients in Riyadh, Kingdom of Saudi Arabia

This study is aimed at evaluating the association between Coronavirus disease 19 (COVID‐19) and the primary complete blood count (CBC) parameters in confirmed positive patients. In a retrospective cross‐sectional study, 384 files of patients with confirmed COVID‐19 diagnosis hospitalized at King Saud Medical City, Riyadh, were chosen randomly as a study group for hematological parameters, with another 384 non‐COVID‐19 files of patients without history of any disease which could influence the hematological profile were selected as a control group. The gender, age, and nationality of the control group were matched with those of the study group. Anemia and thrombocytopenia prevalence was significantly higher in COVID‐19 positive patients compared with negative. However, the positive cases were 3.4 times more likely to be anemic and approximately 5.3 times as likely to be thrombocytopenic, while the prevalence of leukopenia showed no statistical significance between the two groups. However, the Mean Cell Volume (MCV), Total White Blood Cell (WBC) count, lymphocyte count, and basophil count median values showed a nonsignificant difference between the two groups. Anemia and thrombocytopenia may be highly developed in severe positive cases, and therefore, further studies are recommended to validate these findings.

Effect of gold nanoparticles shape and dose on immunological, hematological, inflammatory, and antioxidants parameters in male rabbit.

Background and Aim:Gold nanorods (AuNRs) have gained much attention recent years due to their promising optical and chemical properties and are hence used in applied research and industrial nanotechnology. This study was designed to investigate the effect of gold nanoparticle shape (Gold nanorods vs. gold nanosphere) on immune response in rabbit.Materials and Methods:Thirty New Zealand white rabbits were divided into six groups (n=5 rabbits). The first group is the control negative received an intravenous (IV) injection of normal saline 0.9%; the second group (vaccinated) is the control positive, and the other four groups were vaccinated and received a single-dose or repeated five consecutive IV doses of 300 mg/kg body weight 50 nm AuNRs or 50 nm gold nanosphere (50 nm AuNSs) dissolved in ultrapure water. Blood and serum were collected for the hematological and biochemical analysis.Results:White blood cells (WBCs) count, lymphocytes, monocytes, eosinophils, and basophils showed significantly (p<0.05) higher values with the repeated-dose AuNRs. g-globulin levels showed a significant difference after 15 days in the single-dose AuNSs. Single-dose AuNSs significantly (p<0.05) increased the immunoglobulin G (IgG) and significantly (p<0.05) decreased the tumor necrosis factor-alpha. In addition, it elicited a significant (p<0.05) decrease in the malondialdehyde levels and a significant (p<0.05) increase of the superoxide dismutase, glutathione peroxidase, and catalase levels. Moreover, evoked red blood cells count, mean corpuscular volume, and mean corpuscular hemoglobin were significantly (p<0.05) lower than the control group. The platelet count, lysozymes, and nitric oxide were significantly (p<0.05) higher in repeated-dose AuNRs.Conclusion:The effect of AuNPs is shape and dose-dependent. The repeated 5 days IV 50 nm AuNRs doses over 15 days showed a significant antioxidant effect, with no considerable toxicity or vascular reactions.