Basic Structural Units Research Articles

The Phlebovirus Ribonucleoprotein: An Overview.

In negative strand RNA viruses, ribonucleoproteins, not naked RNA, constitute the template used by the large protein endowed with polymerase activity for replicating and transcribing the viral genome. Here we give an overview of the structures and functions of the ribonucleoprotein from phleboviruses. The nucleocapsid monomer, which constitutes the basic structural unit, possesses a flexible arm allowing for a conformational switch between a closed monomeric state and the formation of a polymeric filamentous structure competent for viral RNA binding and encapsidation in the open state of N. The modes of N-N oligomerization as well as interactions with vRNA are described. Finally, recent advances in tomography open exciting perspectives for a more complete understanding of N-L interactions and the design of specific antiviral compounds.

Effect of hydrostatic pressure on the supramolecular assembly of surfactant-cyclodextrin inclusion complexes.

The supramolecular assembly of simple colloids into complex, hierarchical structures arises from a delicate interplay of short-range directional and isotropic long-range forces. These assemblies are highly sensitive to environmental changes, such as temperature variations and the presence of specific molecules, making them promising candidates for nanomachine design. In this study, we investigate the effect of hydrostatic pressure, up to 1800 bar, on the supramolecular assemblies of cyclodextrin/surfactant complexes. Using small-angle neutron scattering, we demonstrate that while the overall structure of the supramolecular aggregates remains largely stable under pressure, the stiffness of the planar lattice formed by the inclusion complexes, the basic structural unit of the supramolecular assemblies, shows a fourfold increase between 250 and 1000 bar. These findings suggest that high-pressure studies can be exploited to better understand the mechanisms of supramolecular assembly processes, thereby aiding in the design of more robust and functional systems.

Analysis on Sustainable Development of Communities in Guilin City—Taking H Community in Guilin City as An Example

As the basic structural unit of the city, As the basic structural unit of a city, communities have a direct impact on the sustainability and environmentally friendly development of the city. This article takes the H Community of Guilin City as an example to conduct an in-depth analysis of sustainable development at the community level. Through the interview survey method, the study found that Guilin H community has made some progress in sustainable development, but there are still some problems in the sustainable development of the community. The main problems involve imperfect systems related to sustainable development management of the community, property management The concept of sustainable development is not clear, and community residents do not agree with the sustainable development concept of property management. In order to achieve a more comprehensive and balanced sustainable development, H community needs to improve the relevant systems of community sustainable development management, clarify the sustainable development concept of property management, and actively improve community residents' understanding of the sustainable development concept of property management. Through the analysis of H community, this article aims to provide experience for other urban communities and jointly promote the sustainable development of urban communities.

Oscillatory large-scale circulation in liquid-metal thermal convection and its structural unit

In Rayleigh–Bénard convection, the size of a flow domain and its aspect ratio $\varGamma$ (a ratio between the spatial length and height of the domain) affect the shape of the large-scale circulation. For some aspect ratios, the flow dynamics includes a three-dimensional oscillatory mode known as a jump rope vortex (JRV); however, the effects of varying aspect ratios on this mode are not well investigated. In this paper, we study these aspect ratio effects in liquid metals, for a low Prandtl number ${{Pr}}=0.03$ . Direct numerical simulations and experiments are carried out for a Rayleigh number range $2.9 \times 10^4 \leq {{Ra}} \leq 1.6 \times 10^6$ and square cuboid domains with $\varGamma =2$ , $2.5$ , $3$ and $5$ . Our study demonstrates that a repeating pattern of a JRV encountered at aspect ratio $\varGamma \approx 2.5$ is the basic structural unit that builds up to a lattice of interlaced JRVs at the largest aspect ratio. The size of the domain determines how many structural units are self-organised within the domain; the number of the realised units is expected to scale as $\varGamma ^2$ with sufficiently large and growing $\varGamma$ . We find the oscillatory modes for all investigated $\varGamma$ ; however, they are more pronounced for $\varGamma =2.5$ and $5$ . Future studies for large-aspect-ratio domains of different shapes would enhance our understanding of how the JRVs adjust and reorganise at such scaled-up geometries, and answer the question of whether they are indeed the smallest superstructure units.

Microscale tissue engineering of liver lobule models: advancements and applications.

The liver, as the body's primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These liver lobules serve as the basic structural and functional units of the liver, comprised of central veins, hepatic plates, hepatic sinusoids, and minute bile ducts. Meanwhile, within liver lobules, distinct regions of hepatocytes carry out diverse functions. The in vitro construction of liver lobule models, faithfully replicating their structure and function, holds paramount significance for research in liver development and diseases. Presently, two primary technologies for constructing liver lobule models dominate the field: 3D bioprinting and microfluidic techniques. 3D bioprinting enables precise deposition of cells and biomaterials, while microfluidics facilitates targeted transport of cells or other culture materials to specified locations, effectively managing culture media input and output through micro-pump control, enabling dynamic simulations of liver lobules. In this comprehensive review, we provide an overview of the biomaterials, cells, and manufacturing methods employed by recent researchers in constructing liver lobule models. Our aim is to explore strategies and technologies that closely emulate the authentic structure and function of liver lobules, offering invaluable insights for research into liver diseases, drug screening, drug toxicity assessment, and cell replacement therapy.

Enhanced dechlorination of 2,4-dichlorophenol using nanorod palygorskite-loaded Fe/Ni nanoparticles: Performance evaluation, influencing factors and action mechanism of Fe and Ni

The basic structural unit of palygorskite (P) consists of rod-shaped crystals with a diameter ranging from 20 to 70 nm. However, due to strong hydrogen bonding and electrostatic forces, most palygorskite rod crystals tend to aggregate, limiting its nanomaterial properties in practical application. To address this limitation, the study focused on dissociating palygorskite aggregates and incorporating them into nanorod palygorskite-loaded Fe/Ni bimetallic nanoparticles (nP-nFe/Ni) synthesis. This approach facilitated the dissociation of palygorskite aggregates and enabled the dispersion of Fe/Ni nanoparticles by palygorskite nanorods. The results revealed that the BET specific surface area of nP-nFe/Ni was significantly higher at 86.17 m2/g compared to 33.62 m2/g for nFe/Ni. Consequently, the dechlorination efficiency of nP-nFe/Ni for 2,4-dichlorophenol (2,4-DCP) at 120 min was 22.4% higher than that of nFe/Ni, despite nP-nFe/Ni containing only 35.1% of the Fe content found in nFe/Ni at the same dosage. Moreover, it was observed that acidic conditions favor maximum dichlorination, while higher temperatures enhance the dechlorination rate. The action mechanism of Fe was identified as the generation of H2, which is catalyzed by Ni to form active hydrogen (H*) that attack the C-Cl bonds of 2,4-DCP for hydrodechlorination. Ni played a vital role in catalyzing H2 conversion to H* , accelerating H2 production by Fe, and inhibiting Fe passivation. These findings are crucial for the application of nano iron technology and nano-scale palygorskite in water and wastewater treatment.

Mechanistic analysis of chemical structure evolution for coals under igneous intrusion

Thermally altered coals (TACs) have different chemical properties compared to normally buried coals owing to the influence of igneous intrusion. This discrepancy in chemical components of TACs has not been fully revealed, which leads to their underutilization and potential risks in exploitation. Igneous intrusion causes the rearrangement of aromatic rings and the formation of graphite-like features in TACs. Several studies have examined the changes in coal rank and aromaticity during this process but have not explored the overall evolution. In particular, the lateral extension and vertical stacking of aromatic layers in TACs during graphitization are still unknown. To this aim, a range of TACs was collected, from bituminous to semi-graphite. Four experimental analyses were conducted to characterize the chemical structure evolution of TACs, including Raman spectroscopy, Fourier-transform infrared spectrometer (FTIR), X-ray photoelectron spectroscopy (XPS), and high-resolution transmission electron microscopy (HRTEM). The analysis of the measured parameters indicates that the chemical structure of TACs undergoes evolution as it approaches igneous intrusion. These changes involve an increase in the size of the aromatic structural units, a reduction in structural defects, and an increase in the degree of order. In addition, there is a gradual transition of sp2-sp3 hybridized aromatic rings to sp2 hybridized aromatic rings, resembling the process of graphite formation. The HRTEM micrographs also reveal an enlargement in the size and angular orientation of aromatic fringes. In summary, the evolution of TACs can be categorized into three stages: a) rapid growth of basic structural units (BSU) from bituminous coal to anthracite; b) lateral connections of BSUs from anthracite to meta-anthracite; and c) lateral straightening of BSUs from meta-anthracite to semi-graphite. Understanding this evolutionary mechanism is highly significant in analyzing the formation of pores and in enhancing the utilization and safety of TACs.

Expulsion of small molecule hydrocarbons and expansion of nanopores effect in tectonically deformed coal evolution

This work aimed to investigate the impact of the evolution of small molecular structures during the deformation of coal on the constraints imposed on nanoscale pores and adsorption behaviors. Both undeformed and tectonically deformed coal (TDC) from the Huaibei mining area in China were collected for analysis. The pyridine was employed for the extraction of small molecules from coal, which was further complemented by Fourier-transform infrared spectroscopy (FTIR) and low-pressure CO2 and N2 adsorption experiments (LP-CO2/N2AD). The study yielded the following results: (1) Intense tectonic stresses facilitate the removal and recombination of small molecular side chains within the basic structural units (BSU) of coal. As deformation occurs, hydroxyl groups, aliphatic hydrocarbons, C=O, C=C, aromatic hydrocarbons, and 'C' structures tend to accumulate, resulting in an enhanced extraction rate. (2) The deformation process of the coal demonstrates a significant pore-expansion effect, with the most substantial increase observed in micropores (surface area increased by over 70 %), followed by mesopores and macropores. This enlargement facilitates the aggregation of small molecules and gas storage. (3) Intense deformation stages involving shear slip, ductile deformation, and maceral fragmentation result in small molecular fractures and an increased number of micropores. The removal and recombination of I, DOC, and CH2/CH3 within BSUs lead to the formation of larger interconnected pores accompanied by the aggregation of hydroxyl groups (OH–), C=O, C=C, aliphatic hydrocarbons, and 'C' structures. (4) The adsorption capacity of residual coal is lower than that of the raw coal before extraction, indicating that small molecular structures contribute to increased adsorption. This likely plays a significant role in the higher gas content observed in structurally deformed coal. The removal of small molecular structures effectively diminishes the adsorption capacity, providing novel perspectives for coalbed methane development and gas extraction in TDC areas.

Неотерический взгляд на sp2 аморфный углерод

Presented is a concentrated synopsis of facilities of empirical and virtual analytics that, once applied, have provided a fully new vision of sp2 amorphous carbons. This study proved that the solids are multilevel structures, started with the first-level basic structural units (BSUs) and accomplished as macroscopic agglomerates of globular structures, consisting, in its turn, of stacked BSUs. BSUs present necklaced graphene molecules, size, and shape of which are governed by the relevant graphene domains while chemical composition in addition to basic carbon is controlled with heteroatoms of the necklaces. This study shows that BSUs and stacks of BSUs determine the short-range order of the solids and are the main subject of the applied analytics. The synopsis consists of two parts related to empirical and virtual analytics. The former is composed of sections related to structural determination, total and atomic chemical content evaluation and elicitation of the covalent bond composition. The second presents new analytic approaches based on the Digital Twins concept and virtual vibrational spectrometry. The synopsis is configured as an atlas composed of generalized pictures accompanied with necessary explanations to be discussed in detail in the extended references.

Epigenetic profiles of elevated cell free circulating H3.1 nucleosomes as potential biomarkers for non-Hodgkin lymphoma

During cell death, nucleosomes, the basic structural unit of chromatin, are released into the blood stream and elevated levels have been found in the plasma of patients with solid cancers. In this study, we demonstrate an increase in cell free circulating H3.1-nucleosomes levels in plasma samples from patients with hematological malignancy, non-Hodgkin lymphoma (NHL), relative to healthy donors. As histone post-translational modifications (PTMs) of circulating nucleosomes are described as potential biomarkers of various solid cancers, we investigated the epigenetic profile of nucleosomes from NHL patients following nucleosome enrichment (Nu.Q® capture) combined with mass spectrometry. Eight histones PTMs, including the acetylation of histone H3 at lysine 9, 14 and 18 as well as the methylation state of histone H3 at lysine 9, 27 and 36, were identified at a higher level in the plasma of NHL patients compared to healthy donors. These results were confirmed in a larger clinical cohort by immunoassay. Subsequently, the temporal profile of these histone PTMs in NHL patients undergoing treatment course highlighted the potential use of these new biomarkers to monitor treatment response and/or disease progression. Our results substantiate that levels of H3.1-nucleosomes are particularly elevated in NHL patients and may be a useful diagnostic tool. Moreover, our work emphasizes the crucial roles of the epigenetic marks present on circulating nucleosomes to detect and monitor tumor progression and/or treatment response of non-Hodgkin Lymphoma.

Enrichment of Coal-Hosted Graphite Deposits Caused by Magmatic Heat Transfer and Tectonic Stress at Feng County, Western Qinling Orogen, China

China has ranked first worldwide in graphite imports in recent years, facing a graphite supply risk. Coal-hosted graphite is the focus of future graphite deposit exploration. The current research on the enrichment and mineralization mechanism of coal-hosted graphite is superficial, and the identification standard of coal-hosted graphite is incomprehensive, restricting the exploration of coal-hosted graphite mineral resources and the development of coal metamorphic evolution theory. In this study, the Caotangou–Meigoucoal-hosted graphite deposit in western Qinling Mountain was taken as a case study for dissection. Based on the data from 1/50,000 and 1/200,000 regional geological mapping and the data of graphite mines in the study area, the samples were systematically collected and analyzed to explore the mechanism of coal graphitization through a 1:5000 geological profile survey, 1/10,000 geological mapping in key areas, and the investigation and cataloguing of abandoned coal-hosted graphite adit. The result was that there were two main coal-hosted graphite ore bodies, striking from nearly east to west. The Rmax values of the samples were 7.23–8.15%, the average values of Vdaf were around 5.0%, the d002 value of the II ore body was 0.3433–0.3389 nm, the d002 value of the I ore body was mainly 0.3418–0.3429 nm, the graphitization degree G value of the II ore body was 8.14–59.30%, the graphitization degree G value of the II ore body was 12.79–25.58%. The II ore body was coal-hosted graphite, while some samples of the I ore body were coal-hosted graphite, and some samples were coal. The magmatic heat controls the thermal metamorphism of coal seams to form graphite. The closer the distance to the magma body, the larger the crystals, and the higher the euhedral degree, indicating the higher degree of coal seam metamorphism. The nearly north–south compressive structures mainly provided effective tectonic stress for the evolution of coal graphitization during the Yanshan period; the basic structural units (BSUs) rotated and rearranged, eventually forming a straight graphite structure, and tectonic stress catalyzed the graphitization process. The coal-hosted graphite deposits formed under the dual effects of magmatic heat transfer and tectonic stress.

Contribution of soil aggregate particle size to organic carbon and the effect of land use on its distribution in a typical small watershed on Loess Plateau, China

Soil organic carbon (SOC) is an important pool in the global carbon cycle, playing a vital role in moderating atmospheric CO2 concentrations. Soil is the largest carbon pool in terrestrial ecosystems and, as the basic unit of soil structure, soil aggregates are key to protecting soil carbon pools. However, the influence of soil aggregate particle size, SOC distribution, and the contribution of different particle sizes to SOC is still unclear, particularly under different land use types. In this study, soil samples were collected from five land use types (slope farmland (SF), forest (FL), grassland (GL), shrubland (SL), and terraced field (TF)) in a typical small watershed in the Loess Plateau, China. We analyzed the soil aggregate particle size, composition, and stability after dry and wet sieving, SOC content of different particle sizes in aggregates, and the effects of different aggregate particle sizes and land use on the distribution of SOC. The results showed that, the surface soil (0 ∼ 20 cm) water-stable aggregates were relatively stable, particularly under FL, the mean weight diameter (MWD) value was 2.16 mm. Deep soil (40 ∼ 60 cm) non-water stable aggregates were more stable, and GL was optimal, the MWD value was 3.94 mm. SOC and total nitrogen (TN) were significantly correlated with water-stable aggregate stability indicators (p < 0.01). The SOC content in the small aggregates (0.25 ∼ 2 mm) was the highest and lowest in the microaggregates (<0.25 mm) under different land use. The carbon/nitrogen (C/N) ratio of microaggregates was significantly higher in SF (the C/N ratio was 23.17) and GL (the C/N ratio was 31.04) than in other land uses (p < 0.01) in the 20–40 cm soil layer. In surface soil, small aggregates contributed>50% to SOC in SF and TF. In deep soil, small aggregates under all five land uses made the largest contribution rate to SOC at 57%. These findings indicate that carbon sequestration in the study area can be improved by a combination of appropriate soil management with ecological construction to increase the content of small aggregates, strengthen the fixation and protection of SOC, and reduce carbon emissions in the soil.

In-Depth Characterization of Sphingoid Bases via Radical-Directed Dissociation Tandem Mass Spectrometry.

Sphingoid base (SPH) is a basic structural unit of all classes of sphingolipids. A sphingoid base typically consists of an aliphatic chain that may be desaturated between C4 and C5, an amine group at C2, and a variable number of OH groups located at C1, C3, and C4. Variations in the chain length and the occurrence of chemical modifications, such as methyl branching, desaturation, and hydroxylation, lead to a large structural diversity and distinct functional properties of sphingoid bases. However, conventional tandem mass spectrometry (MS/MS) via collision-induced dissociation (CID) faces challenges in characterizing these modifications. Herein, we developed an MS/MS method based on CID-triggered radical-directed dissociation (RDD) for in-depth characterization of sphingoid bases. The method involves derivatizing the sphingoid amine with 3-(2,2,6,6-tetramethylpiperidin-1-yloxymethyl)-picolinic acid 2,5-dioxopyrrolidin-1-yl ester (TPN), followed by MS2 CID to unleash the pyridine methyl radical moiety for subsequent RDD. This MS/MS method was integrated on a reversed-phase liquid chromatography-mass spectrometry workflow and further applied for in-depth profiling of total sphingoid bases in bovine heart and Caenorhabditis elegans. Notably, we identified and relatively quantified a series of unusual sphingoid bases, including SPH id17:2 (4,13) and SPH it19:0 in C. elegans, revealing that the metabolic pathways of sphingolipids are more diverse than previously known.

Microstructure changes induced by adsorption/desorption of low rank coals and its desorption hysteresis mechanism

Adsorption/desorption behavior and its hysteresis phenomenon of coalbed methane (CBM) make a critical contribution to estimate CBM recovery. Hence, to investigate the adsorption/desorption mechanism of low rank coals, this study compared the adsorption/desorption characteristics of methane in different rank coals through isotherm adsorption/desorption experiments and then analyzed the changes in microstructure before and after adsorption/desorption. Thus, the coupling relationship among chemical structure, nanopores and gas adsorption/desorption and the desorption hysteresis mechanism for low rank coals were revealed. Results show that the isosteric adsorption heat and desorption hysteresis coefficient present a decreasing first and then increasing trend with coalification from isotherm adsorption/desorption curves and thermodynamic characteristics. Specially, for low rank coals, as the degree of thermal evolution increases, the desorption hysteresis coefficient increases from 0.27 to 0.39 during low metamorphism stage. Then, compared the nanopore structure distribution and microcrystalline structure characteristics before and after adsorption/desorption, methane adsorption/desorption behavior not only promotes the pore expansion effect of ultra-micropores, but also leads to a swelling effect of basic structure unit (BSU). Furthermore, it can be found that as the BSU increases, the specific surface area (SSA) and pore volume (PV) of nanopores in coal increase, which directly controls the adsorption space. Meanwhile, the adsorption/desorption behavior can also induce the BSU swelling to change nanopore structure distribution, especially micropores and ultra-micropores. Finally, concerning desorption hysteresis effect, its esssence can actually be explained by the energy difference and microstructure. All these findings would provide the theorical guidance for CBM development in low rank coals.

A method locating Cu-Ti-based glass formers using Ni-Zr binary clusters

Formation abilities and mechanical properties are essential for the application of amorphous alloys. In this work, the concept of similar elements has been expanded to similar clusters. Firstly, taking the topologically dense packed clusters Ni-Ni6Zr6 from the Ni-Zr system as the basic structural unit, a series of Cu-Ti-based alloy compositions with high formation ability are parsed and designed. The best composition is Cu46.15Ni7.69Ti32.70Zr13.46 with the cluster expression of Ni-Cu6Zr1.75Ti4.25. Then, the amorphous formation abilities are analyzed from the perspective of the packing degree of clusters and the mixing entropies. Secondly, the crystallization activation energies for characteristic temperatures deduced by Kissinger's approach are analyzed. Finally, the plasticity of Cu-Ti-based amorphous alloys is investigated and Cu46.15Ni7.69Ti32.70Zr13.46 exhibits outstanding fracture strength of 2168 MPa. The results in this paper lay the foundation for further exploration of amorphous alloys with high formation ability, good thermal stability, and high compressive plasticity.

ROCK1 is a multifunctional factor maintaining the primordial follicle reserve and follicular development in mice.

The follicle is the basic structural and functional unit of the ovary in female mammals. The excessive depletion of follicles will lead to diminished ovarian reserve or even premature ovarian failure, resulting in diminished ovarian oogenesis and endocrine function. Excessive follicular depletion is mainly due to loss of primordial follicles. Our analysis of published human ovarian single-cell sequencing results by others revealed a significant increase in rho-associated protein kinase 1 (ROCK1) expression during primordial follicle development. However, the role of ROCK1 in primordial follicle development and maintenance is not clear. This study revealed a gradual increase in ROCK1 expression during primordial follicle activation. Inhibition of ROCK1 resulted in reduced primordial follicle activation, decreased follicular reserve, and delayed development of growing follicles. This effect may be achieved through the HIPPO pathway. The present study indicates that ROCK1 is a key molecule for primordial follicular reserve and follicular development.NEW & NOTEWORTHY ROCK1, one of the Rho GTPases, plays an important role in primordial follicle reserve and follicular development. ROCK1 was primarily expressed in the cytoplasm of oocytes and granulosa cell in mice. Inhibition of ROCK1 significantly reduced the primordial follicle reserve and delayed growing follicle development. ROCK1 regulates primordial follicular reserve and follicle development through the HIPPO signaling pathway. These findings shed new lights on the physiology of sustaining female reproduction.

Effect of supercritical CO2 transient high-pressure fracturing on bituminous coal microstructure

Carbon dioxide phase change fracturing is an effective permeability enhancement technology, and its influence mechanism on the coal microstructure remains to be further investigated. To explore the impact of CO2 fracturing on the coal chemical structure, the self-developed transient high-pressure fracturing of CO2 test platform was independently developed. Based on X-ray photoelectron spectrometer (XPS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD), the elemental morphology, functional group and crystal structure of the two bituminous coal samples before and after fracturing were analyzed. The results showed that the fracturing process resulted in the disruption of hydrogen bonds within coal, leading to a reduction in the cross-linking density of the coal structure. Moreover, it facilitated the breaking of fatty alkyl side chains and oxygen-containing functional groups, causing the detachment of side chains and shortening of the fatty chains. The interlayer spacing of aromatic layers (d200) of fractured coal decreased, and the average transverse dimension (La), the average stack height (Lc) and the aromaticity (fa) increased. With the improvement of aromaticity, the arrangement between aromatic layers was looser. The volume and basic unit structure of coal crystal nucleus became larger, and the flattening degree increased. The research results contribute to the understanding of the change of coal microstructure under supercritical CO2 transient high-pressure fracturing and provide theoretical support for exploring the methane adsorption capacity of fractured coal.

Effects of freeze-thaw cycles on the size distribution and stability of soil aggregate in the permafrost regions of the Qinghai-Tibetan Plateau

As the basic units of soil structure, soil aggregate is essential for maintaining soil stability. Intensified freeze–thaw cycles have deeply affected the size distribution and stability of aggregate under global warming. To date, it is still lacking about the effects of freeze–thaw cycles on aggregate in the permafrost regions of the Qinghai-Tibetan Plateau (QTP). Therefore, we investigated the effects of diurnal and seasonal freeze–thaw processes on soil aggregate. Our results showed that the durations of thawing and freezing periods in the 0–10 cm layer were longer than in the 10–20 cm layer, while the opposite results were observed during completely thawed and frozen periods. Freeze–thaw strength was greater in the 0–10 cm layer than that in the 10–20 cm layer. The diurnal freeze–thaw cycles have no significant effect on the size distribution and stability of aggregate. However, < 0.25 mm fraction dominated wet sieving aggregate with the highest proportion during thawing period, while the < 1 mm fraction reached the highest during completely frozen period in the 10–20 cm layer (P < 0.05). Likewise, the mean weight diameter and water-stable aggregate were decreased during thawing period compared with the other periods, which were influenced by soil microbial biomass carbon and belowground biomass. Hence, the seasonal freeze–thaw processes destroyed macro-aggregate (> 0.25 mm) and reduced aggregate stability. Our study has scientific guidance for evaluating the effects of freeze–thaw cycles on soil steucture and provides a theoretical basis for further exploration on soil and water conservation in the permafrost regions of the QTP.

Study on Shear Behavior and Mechanism Based on Shear Functional Unit of Loess Microstructure

The structural specificity and hydrological sensitivity of loess have a strong impact on its long-term stability and safety. This topic is being actively researched and focuses on the macromechanical behavior of the shear strength of loess disturbed and its micromechanisms from the perspective of the dry–wet cycle (especially involving soluble salt erosion). In this paper, the correlation between micro-structural shear functional units and macroscopic degradation behavior was established by combining the changes in physicochemical properties of mass loss, surface cracking, strength deterioration, and structural disturbance of the loess with scanning electron microscopy (SEM) microscopic images in different dry–wet cycles and different salt contents. Results revealed that with the increase in dry–wet cycles and salt content, the mass loss of soil deteriorated and the surface crack rate increased. The cohesion of soil showed an overall decreasing trend, which decreased more obviously in the early stage of the dry–wet cycle, followed by a slow decrease, and tended to be constant after nine dry–wet cycles. However, the internal friction angle increased and then decreased during the whole cycle, and its value generally changed little. According to the deterioration and decay of shear strength, it can be concluded that the structural disturbance of loess increased with the increase in dry–wet cycles and salt content. At the same time, further linear quantization fitting of the structural disturbance parameters showed that the structural parameters had a positive correlation with salt content and a power function with dry–wet cycles, where dry–wet cycles seemed to play a dominant role in the loess structural deterioration rather than salt content. The microscopic study demonstrates that the dry–wet cycles and salt content do not directly affect the cohesion and internal friction angle of soil but change the basic shear structural unit of aggregate and then cause an essential impact on c and φ, which in turn have an essential impact on soil strength attenuation. This paper not only helps to elucidate the essence of water–soil–salt structural interactions but also provides theoretical references for sustainable development research in environmental engineering, geological engineering, and other related fields.

Response of Soil Aggregate Stability to Phosphorus, Nitrogen, and Organic Fertilizer Addition: A Meta-Analysis

Soil is a highly significant resource for human survival, and agglomerates, as the basic unit of the soil structure, not only enhance the soil fertility and control the biological validity of nutrients but also strengthen the soil’s erosion resistance. The mass application of fertilizers may significantly affect crop growth and the soil structure, and the rational application and dispensing of fertilizers will be an urgent issue to be addressed. Therefore, the effect of fertilizer application on the stability of water-stable soil aggregates needs to be studied under different meteorological and soil conditions to draw more general and feasible conclusions. Our meta-analysis of data from 220 independent observations from 56 published studies found that fertilizer application increased the mean weight diameter (MWD) by an average of 18% compared to the no-fertilizer treatment. Among the nitrogen (N), phosphate (P), and organic (OM) fertilizer treatments, the organic fertilizer treatment had a more significant stimulatory effect on the MWD (26%). Among the different fertilizer levels, a low level of phosphorus (&lt;40 kg·ha−1 yr−1), a high level of N (&gt;120 kg·ha−1·yr−1), and a low level of organic fertilizer (&lt;5000 kg·ha−1·yr−1) increased the MWD by 19%, 14%, and 41%, respectively. Across the soil types and land use types, the response to the MWD was positive for red soils and paddy fields, and the organic fertilizer’s stimulatory effect was more significant than that of the chemical fertilizer. The correlation analysis showed that the response rate of the MWD was negatively correlated with the response rate of the soil pH and bulk density (BD) and positively correlated with the response rate of the soil organic carbon (SOC) and microbial mass carbon (MBC). Meanwhile, the partial least square structural equation model (PLS-SEM) showed that the meteorological factors were the main factors affecting the stability of the soil aggregates, while the secondary factors were the soil’s physical and chemical properties. Therefore, this study found that the long-term use of organic fertilizer instead of partial fertilizer is better than the use of chemical fertilizer alone, while more attention should be paid to the influence of temperature and rainfall on the stability of fertilizer in aggregate soil in the future.