Basal Defense Research Articles

Reading Plato's Dialogues to Enhance Learning and Inquiry: Exploring Socrates' Use of Protreptic for Student Engagement by Mason Marshall

Reviewed by: Reading Plato's Dialogues to Enhance Learning and Inquiry: Exploring Socrates' Use of Protreptic for Student Engagement by Mason Marshall William Perrin MARSHALL, Mason. Reading Plato's Dialogues to Enhance Learning and Inquiry: Exploring Socrates' Use of Protreptic for Student Engagement. New York: Routledge, 2021. 223 pp. Cloth, $136.00; paper, $39.16 One doesn't need to search to find criticism of contemporary democratic citizens. We are told we are an ignorant, dogmatic, and generally vicious bunch. These traits, it's natural to think, make trouble for our democracy. One solution, Mason Marshall argues, is to read Plato to reform our vicious tendencies. This might be surprising, considering Plato is sometimes portrayed as a great enemy of democracy. But Marshall doesn't propose to read Plato to find out what Plato thought, or to mine his dialogues for promising arguments. Rather, Marshall proposes to read Plato to develop a theory of philosophical protreptic. "Protreptic," as used by Marshall, means to turn someone toward a new end. And thus, philosophical protreptic is turning someone "so that they want truth more than anything else." The idea, then, is to use Plato to protrepticize students, in order to cultivate democracy-required character traits in students. There are, broadly speaking, two parts to the book. Marshall, in one part, sketches two ways of carrying out his project, a top-down approach (chapter 1) and a bottom-up approach (chapter 2), before putting the project in action to illustrate its value (chapter 5). In the second part of the book, Marshall defends his project from the charge that it's an illegitimate approach to Plato scholarship (chapter 3) and from the charge that his approach would not be sufficiently valuable (chapter 4). Surprisingly, given the stated aim of the book, chapters 3 and 4 take up the bulk of the pages. I offer an explanation of this striking feature below. But first, I follow Marshall's structure in summarizing the two parts. Marshall begins with the top-down approach. The reader would suppose that above all else Socrates aims to foster self-examination in his interlocutors, before imagining various strategies Socrates could have pursued to satisfy this end. The reader evaluates the various imagined strategies, not for whether they are the ones Socrates uses in the text but for how well they would achieve the end. For example, one might think that Socrates, in order to protrepticize Euthyphro, must convince Euthyphro that he needs to be able to cogently answer philosophical questions, if only so that he does not look foolish when facing them. The reader would then sketch thought experiments varying along the axis of harshness—how abrasive Socrates' humiliation of Euthyphro must be—before evaluating which scenario would most likely lead to self-examination. The bottom-up approach proceeds in the opposite order: One starts by wondering what the intermediate ends motivating Socrates' actual behavior in the dialogues could be. For example, one would ask why Socrates muddies the waters in offering convoluted definitions of piety in the Euthyphro (11e7–12d4). It could be that Socrates wants to make Euthyphro look silly, that Socrates wants to overwhelm him, or that Socrates wants Euthyphro to agree to a view Socrates is anxious to refute. It could be all three. But the idea is to identify the strategies that make the [End Page 353] most sense of the supposition that Socrates aims, above all else, to foster self-examination, before evaluating them for their likelihood of turning someone to self-examination. Marshall, in the second part of the book, defends his approach from the objection that, since his project isn't aiming to articulate the content of Plato's mental states, or mining for arguments, he is co-opting Plato illegitimately. The basic defense is that we would have to know much more about Plato's intentions than we do for the charge to stick. In short, given that we are largely unable to articulate the assumptions we have regarding what goods an interpretation of a text will win us, we will be unable to arbitrate between radically different, yet internally coherent, interpretations of Plato. It is initially puzzling why Marshall spends...

Genetic requirements for infection-specific responses in conferring disease resistance in Arabidopsis.

Immunity in plants arises from defense regulatory circuits that can be conceptualized as modules. Both the types (and isolates) of pathogen and the repertoire of plant receptors may cause different modules to be activated and affect the magnitude of activation. Two major defense enzymes of Arabidopsis are ALD1 and ICS1/SID2. ALD1 is an aminotransferase needed for producing the metabolites pipecolic acid, hydroxy-pipecolic acid, and possibly other defense signals. ICS1/SID2 produces isochorismate, an intermediate in the synthesis of salicylic acid (SA) and SA-derivatives. Metabolites resulting from the activation of these enzymes are found in petiole exudates and may serve as priming signals for systemic disease resistance in Arabidopsis. Mutants lacking ALD1 are known to have reduced SA accumulation. To further investigate the role of ALD1 in relation to the SA-related module, immunity phenotypes of double mutants that disrupt ALD1 and ICS1/SID2 or SA perception by NPR1 were compared with each single mutant after infection by different Pseudomonas strains. Exudates collected from these mutants after infection were also evaluated for their ability to confer disease resistance when applied to wild-type plants. During infection with virulent or attenuated strains, the loss of ALD1 does not increase the susceptibility of npr1 or sid2 mutants, suggesting the main role of ALD1 in this context is in amplifying the SA-related module. In contrast, after an infection that leads to strong pathogen recognition via the cytoplasmic immune receptor RPS2, ALD1 acts additively with both NPR1 and ICS1/SID2 to suppress pathogen growth. The additive effects are observed in early basal defense responses as well as SA-related events. Thus, there are specific conditions that dictate whether the modules independently contribute to immunity to provide additive protection during infection. In the exudate experiments, intact NPR1 and ICS1/SID2, but not ALD1 in the donor plants were needed for conferring immunity. Mixing exudates showed that loss of SID2 yields exudates that suppress active exudates from wild-type or ald1 plants. This indicates that ICS1/SID2 may not only lead to positive defense signals, but also prevent a suppressive signal(s).

Defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress: A contradiction between attraction and resistance

Heavy metal pollution, as a common and serious environmental problem worldwide, has been regarded as an abiotic stimulus that can affect plant insect resistance and pest occurrence. This study evaluated the defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress, with consideration given to chemical defense, physical defense, and elemental defense. Our results showed that the H. cunea larvae had a strong preference for Cd-treated F. mandshurica seedlings, but there was a significant reduction in body weight and survival rate in larvae that fed on leaves of Cd-treated seedlings. Under Cd treatment, the increase in attractant metabolites (e.g., styrene, dibutyl phthalate, and d-limonene) and the decrease in repellent metabolites (e.g., aromadendrene, heptadecane, and camphene) in leaf volatiles were responsible for the high attractant activity to H. cunea larvae. Based on leaf physicochemical properties, tissue structure, and phenolic acid content, an overall reduction in physical defense, chemical defense and their combination in F. mandshurica seedlings exposed to Cd stress was identified by Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) analysis. Elemental defense occurred in Cd-treated F. mandshurica seedlings, as evidenced by the high concentration of Cd in leaves and H. cunea larvae under Cd treatment. Taken together, these findings demonstrate that under Cd stress, elemental defense replaces the dominant role of basic defense in F. mandshurica seedlings and accounts for the enhanced ability to defend against H. cunea larvae.

AtbZIP62 Acts as a Transcription Repressor to Positively Regulate ABA Responses in Arabidopsis.

The basic region/leucine zipper (bZIP) transcription factor AtbZIP62 is involved in the regulation of plant responses to abiotic stresses, including drought and salinity stresses, NO3 transport, and basal defense in Arabidopsis. It is unclear if it plays a role in regulating plant responses to abscisic acid (ABA), a phytohormone that can regulate plant abiotic stress responses via regulating downstream ABA-responsive genes. Using RT-PCR analysis, we found that the expression level of AtbZIP62 was increased in response to exogenously applied ABA. Protoplast transfection assays show that AtbZIP62 is predominantly localized in the nucleus and functions as a transcription repressor. To examine the roles of AtbZIP62 in regulating ABA responses, we generated transgenic Arabidopsis plants overexpressing AtbZIP62 and created gene-edited atbzip62 mutants using CRISPR/Cas9. We found that in both ABA-regulated seed germination and cotyledon greening assays, the 35S:AtbZIP62 transgenic plants were hypersensitive, whereas atbzip62 mutants were hyposensitive to ABA. To examine the functional mechanisms of AtbZIP62 in regulating ABA responses, we generated Arabidopsis transgenic plants overexpressing 35S:AtbZIP62-GR, and performed transcriptome analysis to identify differentially expressed genes (DEGs) in the presence and absence of DEX, and found that DEGs are highly enriched in processes including response to abiotic stresses and response to ABA. Quantitative RT-PCR results further show that AtbZIP62 may regulate the expression of several ABA-responsive genes, including USP, ABF2, and SnRK2.7. In summary, our results show that AtbZIP62 is an ABA-responsive gene, and AtbZIP62 acts as a transcription repressor to positively regulate ABA responses in Arabidopsis.

Biofilm Formation on Intrauterine Devices in Diabetic and Nondiabetic Patients with Vulvovaginal Candidiasis

Abstract Introduction/Objective Women with diabetes mellitus (DM) are more susceptible to vulvovaginal candidiasis (VVC) than non-diabetics. This could be due to higher glucose levels predisposing VVC by damaging basic host defense mechanisms and stimulating Candida adhesion to vaginal cells. Objectives Investigating the prevalence of Candida albicans and biofilm formation in diabetic and nondiabetic IUD users. Methods/Case Report A total of 325 vaginal swabs and IUDs were enrolled in this study from the Delta region in Egypt. Females with a median age of 17 to 45 years were divided into two groups; diabetic (29.8%) and nondiabetic (70.1%). They were all vaginitis having one of these symptoms; irritation on the surface of the genital tract, uterine cervix ulcer, vaginal bleeding, and acute vaginal exudates. C. albicans were isolated and identified using cultural characters. Biofilm formation by Candida on removed IUDs and swabs was examined by XTT-reduction assay, scanning electron microscope (SEM), and transmission electron microscope (TEM). Antifungal susceptibility to fluconazole and amphotericin B (AMB) to isolated strains and in vitro experimental biofilm formation was performed. Results (if a Case Study enter NA) In diabetic cases, the highest percentage (91.1%) of isolated C.albicans was found among patients with irritation on the surface of the genital tract, whereas the lowest one (83.3%) was in the symptoms of acute vaginal exudates. In nondiabetic cases, the highest percentage of isolated C.albicans (51.7%) was found among patients with symptoms of acute vaginal exudates. In contrast, the lowest one (32.5%) was in the symptoms of irritation on the surface of the genital tract. XTT method illustrated the biofilm formation; SEM revealed a heterogeneous thick biofilm. Biofilm MIC to strains with AMB >1μg\ml was considered resistant, while those for which AMB concentration were ≤1μg\ml were deemed sensitive. Strains with Fluconazol 64 μg\ml were considered resistant, while those for which Fluconazol concentration was ≤8 μg\ml were deemed sensitive. Conclusion There was a significant direct relationship between diabetic cases and C.albicans prevalence in the current study. Furthermore, the ability to form biofilm was found to be the same in diabetic and nondiabetic patients.

Chitosan triggers actin remodelling and activation of defence genes that is repressed by calcium influx in grapevine cells

Defence to pathogens must be specific. In the past, we have dissected early signalling deployed by bacterial elicitors in a grapevine cell system. In the current work, we asked, how defence of fungi differs. Fungal diseases of grapevine pose great challenges for global viticulture and require massive plant protection measures. Plant cells are able to sense chitin, a central component of fungal cell walls and respond by activation of basal defence. We, therefore mapped early defence responses evoked by chitosan, a chitin fragment able to bind to chitin receptors. We found an activation of calcium influx, monitored by extracellular alkalinisation due to a co-transport of protons, remodelling of actin (but not of microtubules), and the activation of transcripts for phytoalexin synthesis, jasmonate-signalling, salicylate signalling, and chitinase. Interestingly, Gadolinium, an inhibitor of calcium influx, can inhibit extracellular alkalinisation in response to chitosan, while the induction of the phytoalexin synthesis transcripts was specifically promoted. In contrast, both DMSO and benzyl alcohol, compounds known to modulate membrane fluidity, partially inhibited the transcript responses to chitosan. We discuss these data with a model, where chitosan deploys signalling culminating in activation of defence related transcripts, but at the same time activates calcium influx that negatively feeds back on the same signal chain, which might be a mechanism to achieve a temporal signature that is rapid, but transient.

OsDWD1 E3 ligase-mediated OsNPR1 degradation suppresses basal defense in rice.

Many ubiquitin E3 ligases function in plant immunity. Here, we show that Oryza sativa (rice) DDB1 binding WD (OsDWD1) suppresses immune responses by targeting O. sativa non-expresser of pathogenesis-related gene 1 (OsNPR1) for degradation. Knock-down and overexpression experiments in rice plants showed that OsDWD1 is a negative regulator of the immune response and that OsNPR1 is a substrate of OsDWD1 and a substrate receptor of OsCRL4. After constructing the loss-of-function mutant OsDWD1R239A , we showed that the downregulation of OsNPR1 seen in rice lines overexpressing wild-type (WT) OsDWD1 (OsDWD1WT -ox) was compromised in OsDWD1R239A -ox lines, and that OsNPR1 upregulation enhanced resistance to pathogen infection, confirming that OsCRL4OsDWD1 regulates OsNPR1 protein levels. The enhanced disease resistance seen in OsDWD1 knock-down (OsDWD1-kd) lines contrasted with the reduced disease resistance in double knock-down (OsDWD1/OsNPR1-kd) lines, indicating that the enhanced disease resistance of OsDWD1-kd resulted from the accumulation of OsNPR1. Moreover, an in vivo heterologous protein degradation assay in Arabidopsis thaliana ddb1 mutants confirmed that the CUL4-based E3 ligase system can also influence OsNPR1 protein levels in Arabidopsis. Although OsNPR1 was degraded by the OsCRL4OsDWD1 -mediated ubiquitination system, the phosphodegron-motif-mutated NPR1 was partially degraded in the DWD1-ox protoplasts. This suggests that there might be another degradation process for OsNPR1. Taken together, these results indicate that OsDWD1 regulates OsNPR1 protein levels in rice to suppress the untimely activation of immune responses.

Evaluating the Role of Exogenously Applied Ascorbic Acid in Rescuing Soybean Plant Health in The Presence of Pathogen-Induced Oxidative Stress.

Charcoal rot, caused by the soilborne hemibiotrophic fungus Macrophomina phaseolina, is a prevalent and economically significant plant disease. It is hypothesized that M. phaseolina induces oxidative stress-mediated senescence in plants. Infection by M. phaseolina results in the host’s accumulation of reactive oxygen species (ROS) that contribute toward basal defense. However, the production of ROS could also lead to cellular damage and senescence in host tissue. This study aimed to determine if ascorbic acid, a ROS scavenging molecule, could quench M. phaseolina-induced hydrogen peroxide (H2O2) generation in a soybean-M. phaseolina pathosystem. In vitro sensitivity tests showed that M. phaseolina isolates were sensitive to L-ascorbic acid (LAA) at concentrations of 10.5 to 14.3 mM based on IC50 (half-maximal inhibitory concentration) data. In planta cut-stem assays demonstrated that pre-treatment with 10 mM of either LAA (reduced form) or DHAA (dehydroascorbic acid; oxidized form) significantly decreased lesion length compared to the non-pretreated control and post-treatments with both ascorbic acid forms after M. phaseolina inoculation. Further, H2O2 quantification from ascorbic acid-pretreated tissue followed by M. phaseolina inoculation showed significantly less accumulation of H2O2 than the inoculated control or the mock-inoculated control. This result demonstrated that M. phaseolina not only induced H2O2 after host infection but also increased ROS-mediated senescence. This study shows the potential of ascorbic acid, an effective ROS scavenger, to limit ROS-mediated senescence associated with M. phaseolina infection.

A conserved type III effector RipB is recognized in tobacco and contributes to Ralstonia solanacearum virulence in susceptible host plants

Ralstonia solanacearum, the causal agent of bacterial wilt, causes devastating diseases in a wide range of plants including potato, tomato, pepper and tobacco. The pathogen delivers approximately 70 type III effectors (T3Es) into plant cells during infection. In this study, we confirmed that a T3E RipB is recognized in tobacco. We further demonstrated that RipB is conserved among R. solanacearum isolates and five different ripB alleles are all recognized in tobacco. The ripB from GMI1000 was transformed into susceptible host Arabidopsis, and a defect in root development was observed in ripB-transgenic plants. Pathogen inoculation assays showed that ripB expression promoted plant susceptibility to R. solanacearum infection, indicating that RipB contributes to pathogen virulence in Arabidopsis. Expression of ripB in roq1 mutant partially suppressed reactive oxygen species production, confirming that RipB interferes with plant basal defense. Interestingly, ripB expression promoted cytokinin-related gene expression in Arabidopsis, suggesting a role of cytokinin signaling pathway in plant-R. solanacearum interactions. Finally, RipB harbors potential 14-3-3 binding motifs, but the associations between RipB and 14-3-3 proteins were undetectable in yeast two-hybrid assay. Together, our results demonstrate that multiple ripB alleles are recognized in Nicotiana, and RipB suppresses basal defense in susceptible host to promote R. solanacearum infection.

Research progress in association between endothelial dysfunction and periodontitis

Vascular endothelium formulates the basic defense against cardiovascular diseases. Multiple factors such as inflammatory factors, oxidative stress and biological factors can cause endothelial dysfunction and be involved in the formation and development of cardiovascular diseases. In studies of recent years, accumulated evidences showed that periodontitis was an independent risk factor for cardiovascular events, and was related to vascular endothelial dysfunction. Periodontal therapy could improve the vascular endothelial function. In this paper, the epidemiological evidences of associations between periodontitis and vascular endothelial dysfunction in recent years were listed, and the possible mechanisms of periodontitis aggravating endothelial dysfunction were analyzed. The importance of periodontal intervention in improving endothelial function was emphasized. This will provide new ideas for further study about the relationship between periodontitis and cardiovascular diseases and for the prevention and treatment strategies.

Development of Media Training Videos Tutorials on Basic Techniques of Karate Martial Arts for Early Ages at Dojo Gaperta

The problem that occurs in the training process is the need for an interesting and fun media so that it can build student motivation and make it easier for students to introduce basic karate techniques. This study aims to produce a product in the form of a CD of video tutorial training media for basic martial arts techniques for early childhood. This research method is research and development that uses ten steps in development research. The development of learning media and video tutorial exercises on basic martial arts techniques were validated by one material expert, one media expert and 10 students for small group trials, 20 students for field trials. The subjects in this study were in the Dojo Gaperta data with a qualitative descriptive approach and a quantitative descriptive approach. Results of research and development: Overall, this karate tutorial video training media with the subject matter (blocking technique) is categorized as suitable for use in karate training for an early age. Based on the trial, the feasibility of the video tutorial training media for basic karate defense techniques for elementary school students grades 1-6 includes: 97.6% in terms of material, 88.25% in terms of media. Small group trial of 88.5% feasibility, field trial of 91.41% feasibility. Overall, the video tutorial training media for basic martial arts techniques for early childhood is appropriate to be used in the introduction and practice of basic karate techniques after going through several trial stages.

Transgenic Expression of Nrf2 Induces a Pro-Reductive Stress and Adaptive Cardiac Remodeling in the Mouse.

Nuclear factor, erythroid 2 like 2 (Nfe2l2 or Nrf2), is a transcription factor that protects cells by maintaining a homeostatic redox state during stress. The constitutive expression of Nrf2 (CaNrf2-TG) was previously shown to be pathological to the heart over time. We tested a hypothesis that the cardiac-specific expression of full length Nrf2 (mNrf2-TG) would moderately increase the basal antioxidant defense, triggering a pro-reductive environment leading to adaptive cardiac remodeling. Transgenic and non-transgenic (NTG) mice at 7–8 months of age were used to analyze the myocardial transcriptome, structure, and function. Next generation sequencing (NGS) for RNA profiling and qPCR-based validation of the NGS data, myocardial redox levels, and imaging (echocardiography) were performed. Transcriptomic analysis revealed that out of 14,665 identified mRNAs, 680 were differently expressed (DEG) in TG hearts. Of 680 DEGs, 429 were upregulated and 251 were downregulated significantly (FC > 2.0, p < 0.05). Gene set enrichment analysis revealed that the top altered pathways were (a) Nrf2 signaling, (b) glutathione metabolism and (c) ROS scavenging. A comparative analysis of the glutathione redox state in the hearts demonstrated significant differences between pro-reductive vs. hyper-reductive conditions (233 ± 36.7 and 380 ± 68.7 vs. 139 ± 8.6 µM/mg protein in mNrf2-TG and CaNrf2-TG vs. NTG). Genes involved in fetal development, hypertrophy, cytoskeletal rearrangement, histone deacetylases (HDACs), and GATA transcription factors were moderately increased in mNrf2-TG compared to CaNrf2-TG. Non-invasive echocardiography analysis revealed an increase in systolic function (ejection fraction) in mNrf2-TG, suggesting an adaptation, as opposed to pathological remodeling in CaNrf2-TG mice experiencing a hyper-reductive stress, leading to reduced survival (40% at 60 weeks). The effects of excess Nrf2-driven antioxidant transcriptome revealed a pro-reductive condition in the myocardium leading to an adaptive cardiac remodeling. While pre-conditioning the myocardial redox with excess antioxidants (i.e., pro-reductive state) could be beneficial against oxidative stress, a chronic pro-reductive environment in the myocardium might transition the adaptation to pathological remodeling.

Abstract P3091: Transgenic Expression Of Nrf2 Induces A Pro-reductive Stress And Adaptive Cardiac Remodeling In Mice

Background: NFE2L2, nuclear factor, erythroid 2 like 2 (Nrf2), is a transcription factor that protects cells through maintaining a homeostatic redox state during stress. Constitutive expression of Nrf2 (CaNrf2-TG) was previously shown to be pathological to the heart over time. We tested a hypothesis that the cardiac-specific expression of full length Nrf2 (mNrf2-TG) would moderately increase the basal antioxidant defense, triggering a pro-reductive environment leading to adaptive cardiac remodeling. Methods: Cardiac-specific full-length Nrf2 transgenic (mNrf2-TG) and non-transgenic (NTG) mice hearts on the C57/BL6 background at 7-8 months of age were used to analyze the transcriptome and cardiac structure/function. Next generation sequencing (NGS) for RNA profiling (n=3/group) and qPCR-based validation (n=5/group) of the NGS data were accomplished. Myocardial redox levels and imaging (echocardiography) were performed. Results: Transcriptomic analysis revealed that out of 14665 identified mRNAs, 679 were differently expressed (DEG) in TG hearts. Of 679 DEGs, 429 were upregulated and 251 were downregulated significantly (FC&gt;2, P&lt;0.05). Gene Set Enrichment Analysis revealed that the top altered pathways were (a) Nrf2 signaling, (b) glutathione metabolism and (c) ROS scavenging. A comparative analysis of glutathione redox state in the hearts demonstrated significant differences between pro-reductive vs. hyper-reductive conditions (233 ± 36.7 and 380 ± 68.7 vs. 139 ± 8.6 μM/mg protein in mNrf2-TG and CaNrf2-TG vs NTG). Expression of fetal genes and hypertrophic biomarkers, cytoskeletal genes, histone deacetylases (HDACs), and GATA transcription factors were moderately increased in mNrf2-TG compared to CaNrf2-TG hearts. Non-invasive echocardiography analysis revealed an increase in systolic function (Ejection Fraction) in mNrf2-TG, suggesting an adaption, as opposed to pathological remodeling in CaNrf2-TG mice experiencing a hyper-reductive stress, leading to reduced survival (40% at 60 weeks). Conclusion: The effects of excess Nrf2-driven antioxidant transcriptome revealed a pro-reductive condition in the myocardium leading to an adaptive cardiac remodeling. While pre-conditioning the myocardial redox with excess antioxidants (i.e. pro-reductive state) could be beneficial against oxidative stress, a chronic pro-reductive environment in the myocardium might transition the adaptation to pathological remodeling.

Provably curb man-in-the-middle attack-based ARP spoofing in a local network

Even today, internet users’ data security remains a significant concern. One problem is ARP poisoning, otherwise referred to as ARP spoofing. Such attacks are intended to exploit the identified ARP protocol vulnerability. Despite no straightforward remedy for ARP spoofing being apparent, certain actions may be taken to maintain one’s safety. The most basic and common defence against a poisoning attack is manually adding MAC and IP addresses to the static ARP cache table. However, this solution is ineffective for large networks where static entries require considerable time and effort to maintain, whether by human input or via special tools and settings for the static entries of network devices. Accordingly, this paper aimed to monitor network packet information and detect the behaviour of ARP poison attacks on operating systems, for instance Windows and Linux. The discovery and defence policy systematically and periodically check the MAC addresses in the ARP table, enabling alerts to be issued if a duplicate entry is detected. This enables the poison-IP address to be blocked before a reply is sent. Finally, the results showed that the superiority was successfully achieved in the detection, prevention and reporting mechanisms in the real-world environment.

Correction to: The Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses.

Correction to: The Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses.

Tuberculosis, BCG Vaccination, and COVID-19: Are They Connected?

Evidence from multiple scientific studies suggests that the Bacillus Calmette-Guérin (BCG) vaccine, widely used worldwide as a preventive measure against tuberculosis, also offers crossprotection against other pathogens. This review aimed to gather data from research that studied the mechanisms involved in the immunological protection induced by the BCG vaccine, which may be important in the control of viral infections, such as COVID-19. Through a literature review, we compiled information about the different BCG strains used worldwide, as well as the responses and protection elicited by them. We commented on the mechanisms of immune response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), and we discussed the possibility of cross-protection of different BCG strains on the control of COVID-19. Due to the immunomodulatory properties of BCG, some BCG strains were able to induce an effective cellular immune response and, through epigenetic modifications, activate cells of the innate immune system, such as monocytes, macrophages and natural killer cells, which are crucial for the control of viral infections. Although several vaccines have already been developed and used in an attempt to control the COVID-19 pandemic, some BCG vaccine strains may help stimulate the basal defences against these pathogens and can be used as additional defences in this and future pandemics.

The E3 ubiquitin ligase RING1 interacts with COP9 Signalosome Subunit 4 to positively regulate resistance to root-knot nematodes in Solanum lycopersicum L.

Globally, root-knot nematodes (RKNs) cause massive production losses in all major crops. E3 ubiquitin ligases are involved in plant growth, development and immune response. But their roles in plant defense against RKNs are largely unclear. Here, we show that tomato E3 ubiquitin ligase RING1 interacts with COP9 Signalosome Subunit 4 (CSN4) which is essential for jasmonic acid (JA)-dependent basal defense against RKNs. Tissue-specific expression analysis showed that RING1 expression was the highest in tomato roots and the expression was significantly increased with RKN (Meloidogyne incognita) infection. Compared with the wild-type plants, the number of egg masses in roots significantly increased in the ring1 mutants, while RING1 overexpression conferred resistance against RKNs. Furthermore, RKN infection increased the accumulation of CSN4 protein in the roots of wild-type plants, which was largely compromised in the ring1 mutants but was enhanced in the RING1 overexpressing plants. The RKN-induced transcripts of JA biosynthetic and signaling genes as well as the accumulation of JA and JA-isoleucine were compromised in ring1 mutants but were increased in RING1 overexpressing plants. These results suggest that RING1 positively regulates JA-dependent basal defense against RKNs by interacting with CSN4 proteins.

GLRaV-2 protein p24 suppresses host defenses by interaction with a RAV transcription factor from grapevine.

Grapevine leafroll-associated virus 2 (GLRaV-2) is a prevalent virus associated with grapevine leafroll disease, but the molecular mechanism underlying GLRaV-2 infection is largely unclear. Here, we report that 24-kDa protein (p24), an RNA-silencing suppressor (RSS) encoded by GLRaV-2, promotes GLRaV-2 accumulation via interaction with the B3 DNA-binding domain of grapevine (Vitis vinifera) RELATED TO ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 (VvRAV1), a transcription factor belonging to the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) superfamily. Salicylic acid-inducible VvRAV1 positively regulates the grapevine pathogenesis-related protein 1 (VvPR1) gene by directly binding its promoter, indicating that VvRAV1 may function in the regulation of host basal defense responses. p24 hijacks VvRAV1 to the cytoplasm and employs the protein to sequester 21-nt double-stranded siRNA together, thereby enhancing its own RSS activity. Moreover, p24 enters the nucleus via interaction with VvRAV1 and weakens the latter's binding affinity to the VvPR1 promoter, leading to decreased expression of VvPR1. Our results provide a mechanism by which a viral RSS interferes with both the antiviral RNA silencing and the AP2/ERF-mediated defense responses via the targeting of one specific host factor.

Overexpression of Rice BSR2 Confers Disease Resistance and Induces Enlarged Flowers in Torenia fournieri Lind

Plant pathogens evade basal defense systems and attack different organs and tissues of plants. Genetic engineering of plants with genes that confer resistance against pathogens is very effective in pathogen control. Conventional breeding for disease resistance in ornamental crops is difficult and lagging relative to that in non-ornamental crops due to an inadequate number of disease-resistant genes. Therefore, genetic engineering of these plants with defense-conferring genes is a practical approach. We used rice BSR2 encoding CYP78A15 for developing transgenic Torenia fournieri Lind. lines. The overexpression of BSR2 conferred resistance against two devastating fungal pathogens, Rhizoctonia solani and Botrytis cinerea. In addition, BSR2 overexpression resulted in enlarged flowers with enlarged floral organs. Histological observation of the petal cells suggested that the enlargement in the floral organs could be due to the elongation and expansion of the cells. Therefore, the overexpression of BSR2 confers broad-spectrum disease resistance and induces the production of enlarged flowers simultaneously. Therefore, this could be an effective strategy for developing ornamental crops that are disease-resistant and economically more valuable.

Rice diterpenoid phytoalexins are involved in defence against parasitic nematodes and shape rhizosphere nematode communities.

Rice diterpenoid phytoalexins (DPs) are secondary metabolites with a well known role in resistance to foliar pathogens. As DPs are also known to be produced and exuded by rice roots, we hypothesised that they might play an important role in plant-nematode interactions, and particularly in defence against phytoparasitic nematodes. We used transcriptome analysis on rice roots to analyse the effect of infection by the root-knot nematode Meloidogyne graminicola or treatment with resistance-inducing chemical stimuli on DP biosynthesis genes, and assessed the susceptibility of mutant rice lines impaired in DP biosynthesis to M. graminicola. Moreover, we grew these mutants and their wild-type in field soil and used metabarcoding to assess the effect of impairment in DP biosynthesis on rhizosphere and root nematode communities. We show that M. graminicola suppresses DP biosynthesis genes early in its invasion process and, conversely, that resistance-inducing stimuli transiently induce the biosynthesis of DPs. Moreover, we show that loss of DPs increases susceptibility to M. graminicola. Metabarcoding on wild-type and DP-deficient plants grown in field soil reveals that DPs significantly alter the composition of rhizosphere and root nematode communities. Diterpenoid phytoalexins are important players in basal and inducible defence against nematode pathogens of rice and help shape rice-associated nematode communities.