Chemical fertilizers are known to improve plant yield but on the cost of environment, soil health as well as human health. Thus, alternatives of chemical fertilizers are the demand of time. Plant growth-promoting fungi (PGPF) are using nowadays as an alternative of chemical fertilizers to improve the growth, yield, and plant nutrient uptake. They are also involved in the improvement of rhizosphere fertility, without compromising environment and human health. A total of seven fungal isolates were obtained from rhizospheric soil of two crop plants viz., turnip and wheat. Fungal strains were examined for their In vitro plant growth promotional traits. A total of two strains found positive for 4 or more plant growth promoting traits. These potential fungal strains were characterized by amplification and sequencing of ITS region and were identified as Trichoderma reesei, and another was identified as Fusarium solani. Our current study is aimed to screen the effect of single inoculation as well as paired fungal inoculations and to provide evidence for the synergistic effects of paired isolates having high impact on growth and development of barley plant.