Barley Cultivars Research Articles

The effects of pre-harvest glyphosate rate and timing on yield and pre-malt quality of malting barley

The production of barley cultivars with malting and brewing quality characteristics is subject to strict grading and technical standards for the end-use market. Environmental and management factors can significantly alter grain quality, and the qualities required for malting. Crop and kernel uniformity are critical factors where variability can exceed the tolerance for meeting malt quality. A practice to address variations in crop maturity is the application of pre-harvest glyphosate. Pre-harvest glyphosate applications can, however, alter malting characteristics in barley, and, if mis-timed, can also reduce yield. A 4-year study at five locations in Alberta and Saskatchewan from 2013 to 2017 was conducted to determine the effects of pre-harvest glyphosate applications on malting barley characteristics. Glyphosate was applied at 900 and 1125 g ae ha−1 on malting barley cultivars ‘CDC Meredith’ and ‘AC Metcalfe’ malting barley at soft dough, hard dough, and physiological maturity growth stages. Yield reductions of up to 12% were observed from glyphosate applications at soft dough, and yield was maximized with applications at physiological maturity. Glyphosate application, at two rates, reduced percentage plump kernels, but did not affect kernel weight or protein concentration. The growth stage of barley plants did not provide an accurate indicator of seed moisture levels at the time of application, which motivated our conclusion that glyphosate applications can be mistimed by inaccurate indicators. The results motivate our questioning of the utility of pre-harvest glyphosate applications, given the adverse effects to barley yield and quality observed, even when applied according to the label instructions.

Field and Economic Evaluation of Barley Productivity as Affected by Seed Rates and Slow-release Nitrogen Fertilizer Levels under Rainfed Conditions

A field experiment was conducted at the north western coast of Egypt under rainfed conditions to study the effects of seed rates (70, 95 and 120 kg ha-1) and nitrogen fertilizer treatments (0, 35, 70 and 105 kg N ha-1) of Ensiabeen-40% N as slow-release fertilizer compared with 105 kg N ha-1 as Ammonium nitrate (33.5%N) on productivity of barley cultivar Giza134. This investigation was conducted during 2019/2020 and 2020/2021 winter growing seasons. The treatments were laid out in a split plot design, with three replications. Plant height, spike length, number of grins spike-1, number of spikes per m2, 1000 grain weight, biological and grain yield were recorded. In addition, total income and net return (LE)/hactare were calculated. The results indicated that mean squares due to seasons, seed rates, nitrogen levels and their interactions were significant for most studied traits. Mean performance of the studied traits as affected by seasons, seed rates and nitrogen levels showed that, the most desirable values were obtained by SR1 for spike length (6.13cm), number of grains spike-1 (48.78 grain) and 1000 grain weight (42.15g), from SR2 for Biological yield (6.02 ton ha-1) and grain yield (1.85 ton ha-1) and from SR3 for plant height (78.28cm) and number of spikes m-2 (180.11 spike). For nitrogen level effects, N4 recorded the most favorable values for all studied traits; plant height (81.48cm), spike length (6.33cm), number of grains spike-1 (49.99 grain), number of spikes m-2 (187.60 spike), Biological yield (6.88 ton ha-1), grain yield (2.12 ton ha-1) and 1000 grain weight (42.89g). Grain yield exhibited highly significant and positive correlation with each of plant height (r= 0.833**), spike length(r=0.621**), number of grains spike-1 (r=0.768**), number of spikes m-2(r=0.880**) and weight of 1000 grains (r= 0.661**). The most contributing variable in the total variation of grain yield were number of spikes m-2, number of grains spike-1 and spike length. These variables contributed by 92.9% in the grain yield variation. The results also indicated that, the highest values in the total income were 19149 and 24990 pound.hec-1 respectively, and net return were 9492 and 15498 pound.hec-1 obtained from the interaction between seed rate of 5kg ha-1 (SR2) and 105 unit of slow release fertilizer (N4) during the two growing seasons. It could recommend this treatment to maximize the total income for the farmers in such location.

Integrative transcript to proteome analysis of barley during Ramularia collo-cygni leaf spot development identified several proteins that are related to fungal recognition and infection responses.

Ramularia leaf spot (RLS) disease is a growing threat to barley cultivation, but with no substantial resistance identified to date. Similarly, the understanding of the lifestyle of Ramularia collo-cygni (Rcc) and the prediction of RLS outbreak severity remain challenging, with Rcc displaying a rather untypical long endophytic phase and a sudden change to a necrotrophic lifestyle. The aim of this study was to provide further insights into the defense dynamics during the different stages of colonization and infection in barley in order to identify potential targets for resistance breeding. Utilizing the strength of proteomics in understanding plant-pathogen interactions, we performed an integrative analysis of a published transcriptome dataset with a parallel generated proteome dataset. Therefore, we included two spring barley cultivars with contrasting susceptibilities to Rcc and two fungal isolates causing different levels of RLS symptoms. Interestingly, early responses in the pathogen recognition phase of the host were driven by strong responses differing between isolates. An important enzyme in this process is a xylanase inhibitor, which protected the plant from cell wall degradation by the fungal xylanase. At later time points, the differences were driven by cultivar-specific responses, affecting mostly features contributing to the pathogenesis- and senescence-related pathways or photosynthesis. This supports the hypothesis of a hemibiotrophic lifestyle of Rcc, with slight differences in trophism of the two analyzed isolates. The integration of these data modalities highlights a strength of protein-level analysis in understanding plant-pathogen interactions and reveals new features involved in fungal recognition and susceptibility in barley cultivars.

Fertilizer regime and cultivar affect barley growth and rhizobiome composition

To combat climate change and environmental pollution, agriculture must reduce mineral fertilizer use and adopt sustainable, low-input, and organic practices. In such systems, plants depend on the soil microbiome for nutrient acquisition and growth. Thus, enhancing plant-microbiome interactions is vital for maintaining or even increasing production sustainably. Modern plant breeding has led to barley cultivars that yield high under substantial mineral fertilizer application. However, this selective breeding may have diminished traits essential for plant-microbiome interactions, making these cultivars less suitable for sustainable agriculture. In contrast, older cultivars might have preserved traits from their common wild ancestor, enabling them to prosper under low nutrient conditions. This study evaluated four modern elite barley cultivars and three older (pre-1980). Grown under various fertilizer regimes, we assessed their root microbiome using 16S rRNA amplicon sequencing. Our objectives were: i) to determine the impact of nutrient availability on plant nutrient uptake and biomass production, and ii) to understand how the time since domestication (domestication age), individual cultivar, and fertilizer regime influence the root microbiome. We found that without fertilizer, old cultivars had superior biomass production and higher leaf concentrations of nitrogen, potassium, sulfur, iron, zinc, and copper than modern cultivars. This indicates that older barley cultivars may have retained their wild ancestors' capability to synergize with the soil microbiome, enhancing nutrient acquisition in low-input systems. Notably, the diversity of the rhizo-microbiome was not significantly affected by domestication age but varied with individual cultivar and fertilizer treatment.

Comparative analysis of wheat and barley yield performance across temperate environments

ContextA comparative analysis of grain yield performance of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) under different environments constitutes a way to identify restrictions in yield formation between both crop species. However, most of the previous studies on these field crops have been carried out in Mediterranean environments. ObjectiveThis study aims to compare the grain yield performance of wheat and barley across a wide range of temperate environmental conditions. MethodsWheat and barley yield databases from the national network of cultivar evaluation of Argentina were assembled. The database included 191 wheat cultivars (with early-, mid- and late-flowering length cycle), 140 barley cultivars, and 44 combinations of sites and years of the Argentine wheat and barley belt region. Average heading date variation among environments and crops was no more than 10 days. Crop grain yield comparisons were carried out using a regression analysis, benchmarking within each site-year the grain yield of each cultivar against the average grain yield of all crop cultivars (i.e., environmental index). ResultsThere were no differences in grain yield between wheat and barley for all data, but the behavior between crop species changed with the length of the wheat cycle (i.e., early-, mid-, or late-flowering cultivars). Barley portrayed a greater grain yield than early-flowering wheat under low-yielding environments (< 6060 kg ha−1), but this advantage vanished as the yield environment improved. Mid-flowering wheat showed a similar grain yield to barley across temperate environments. Lastly, late-flowering wheat outperformed barley mainly in high-yielding environments (> 6138 kg ha−1). Grain number m−2 was the main numerical component that explained variability in grain yield. The relative contribution of the yield numerical components differed between species, having barley lower grain number m−2 but a greater grain weight than wheat. ConclusionsThe comparison of the grain yield performance between species represents a strategy to adjust the rotation system, being a critical factor in considering the variability in the crop growth cycle.

Influence of Foliar Application of Microelements on Yield and Yield Components of Spring Malting Barley

Barley is an economically important plant cultivated primarily for animal feed and in the brewing industry for the production of barley malt. Climate changes and an increase in grain demand result in a constant need to improve the volume and stability of cereal species yields and better use the potential of cultivars. In cereal production, an important aspect is the use of microelements, especially by foliar spraying. Microelements, as components or enzyme activators, play a significant role in plant growth and metabolic processes occurring in the cell. As a consequence, their availability is a factor determining plant development. The aim of this study was to determine the effect of foliar fertilization with selected microelements on the yield of two-row malting barley cultivars. In 2019–2021, a two-factor field experiment with barley was conducted in south-eastern Poland. The experimental factors were three spring barley cultivars (Baryłka, KWS Irina, and RGT Planet) of the brewing type and four single-component micronutrient fertilizers containing copper (Cu), manganese (Mn), molybdenum (Mo), and zinc (Zn). The foliar application of microelements resulted in improvements in selected elements of the yield structure and an increase in grain yield, and the effect depended on the fertilization applied. The highest grain yield was obtained from plots where fertilizer with Mo or Zn was used. Barley plants sprayed with Mo fertilizer developed the longest spikes and were characterized by the highest number of productive tillers per plant. The foliar application of Zn resulted in the formation of the highest number of spikes per unit area and grain uniformity. The RGT Planet cultivar was characterized by higher values of the measured parameters compared to Baryłka and KWS Irina.

Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

BackgroundInterspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids can contribute to the transfer of agronomically useful traits by creating chromosome addition or translocation lines as well as full hybrids. Information on the karyotype of hybrid progenies possessing various combinations of wheat and barley chromosomes is thus essential for the subsequent breeding steps. Since the standard technique of chromosome in situ hybridisation is labour-intensive and requires specific skills. a routine, cost-efficient, and technically less demanding approach is beneficial both for research and breeding.ResultsWe developed a Multiplex Polymerase Chain Reaction (MPCR) method to identify individual wheat and barley chromosomes. Chromosome-specific primer pairs were designed based on the whole genome sequences of ‘Chinese Spring’ wheat and ‘Golden Promise’ barley as reference cultivars. A pool of potential primers was generated by applying a 20-nucleotide sliding window with consecutive one-nucleotide shifts on the reference genomes. After filtering for optimal primer properties and defined amplicon sizes to produce an ordered ladder-like pattern, the primer pool was manually curated and sorted into four MPCR primer sets for the wheat A, B, and D sub-genomes, and for the barley genome. The designed MPCR primer sets showed high chromosome specificity in silico for the genome sequences of all 18 wheat and barley cultivars tested. The MPCR primers proved experimentally also chromosome-specific for the reference cultivars as well as for 13 additional wheat and four barley genotypes. Analyses of 16 wheat × barley F1 hybrid plants demonstrated that the MPCR primer sets enable the fast and one-step detection of all wheat and barley chromosomes. Finally, the established genotyping system was fully corroborated with the standard genomic in situ hybridisation (GISH) technique.ConclusionsWheat and barley chromosome-specific MPCR offers a fast, labour-friendly, and versatile alternative to molecular cytogenetic detection of individual chromosomes. This method is also suitable for the high-throughput analysis of distinct (sub)genomes, and, in contrast to GISH, can be performed with any tissue type. The designed primer sets proved to be highly chromosome-specific over a wide range of wheat and barley genotypes as well as in wheat × barley hybrids. The described primer design strategy can be extended to many species with precise genome sequence information.

Alteration of aluminum tolerance mediated by insertion of same transposable element at different sites in the upstream region of HvAACT1 in Japanese barley accessions

ABSTRACT Aluminum (Al) toxicity is a major limiting factor for crop production in acid soils. Barley is highly sensitive to Al, so the genetic improvement of its Al tolerance is required. However, useful breeding materials and genetic factors for improving its tolerance are remain largely unclear. Here, we compared the Al tolerance of ‘Minorimugi’ and ‘Fibersnow,’ both six-rowed hulled barley cultivars grown mainly in northern Japan, using relative root length (RRL) as an index, and found that Minorimugi had higher tolerance. Quantitative trait locus (QTL) analysis using recombinant inbred lines (RILs) derived from the two cultivars detected a major QTL at the HvAACT1 (Al-ACTIVATED CITRATE TRANSPORTER 1) locus that explained approximately 36% of the variance. The PCR amplification analysis revealed a 1-kb transposable element (TE) insertion at −1.9 kb in the upstream region of HvAACT1 in Minorimugi that enhances HvAACT1 expression. The TE has the same nucleotide sequence as one previously found at −4.8 kb in the upstream region of HvAACT1 in ‘Murasakimochi,’ which is an Al tolerant cultivar. In the Japanese barley population, the Murasakimochi-type HvAACT1 upstream allele is shared mainly among barley accessions developed in the Shikoku area, while the Minorimugi-type allele is shared mainly among accessions developed in the Hokuriku and Nagano area. This geographic difference indicates that both alleles are shared among different subpopulations in Japanese barley, which may be advantageous for growth in acid soils. Our results provide information about a new allele of the HvAACT1 upstream region and potential breeding materials for improving the Al tolerance of barley.

The influence of mitochondria-targeted antioxidants, plastoquinone derivatives on the resistance of grain crops to water deficiency

The article presents the results of a study of the influence of plastoquinone derivatives: 10-(6'-plastoquinonyl)decyltriphenylphosphonium (SkQ1) and 10-(6′-methylplastoquinonyl) decyltriphenylphosphonium (SkQ3) on the resistance of grain crops to soil moisture deficiency. The object of the study were seedlings of spring barley (Hordeum vulgare L.) cultivar “Sokol” and winter wheat (Triticum aestivum L.) cultivar “Kalym”. The growth rates of plants were studied for 14 days under normal conditions and with a lack of moisture, pre-treated with 2.5 nm solutions of SkQ1 and SkQ3. It has been shown that treating seeds with solutions of SkQ1 and SkQ3 increases the growth rate of the studied crops under conditions of lack of moisture compared to the control. Also, in the roots and leaves of 14-day-old barley seedlings, an analysis of the transcriptional activity of the genes SodA1, SodB, GR, GST1, GST6, Cat1, Cat2, Apx1, encoding oxidative stress proteins, was carried out. It was shown that a decrease in soil moisture by 1.8 and 2.0 times compared to the control reduces to varying degrees the weight of roots and leaves in 14-day-old barley seedlings and increases the transcriptional activity of all studied genes in both leaves and roots. Treatment of seeds with solutions of SkQ1 and SkQ3 under conditions of moisture deficiency increases the dry weight of barley roots and leaves and reduces the transcriptional activity of the studied genes in the roots. Keywords: PLASTOQUINONE DERIVATIVES, SkQ1, SkQ3, MITOCHONDRIA-TARGETED ANTIOXIDANTS, ROS, SOIL MOISTURE DEFICIENCY, STABILITY, TRANSCRIPTIONAL GENE ACTIVITY

Virulence Spectra of Hungarian Pyrenophora teres f. teres Isolates Collected from Experimental Fields Show Continuous Variation without Specific Isolate × Barley Differential Interactions.

Pyrenophora teres f. teres (Ptt), the causal agent of net form net blotch (NFNB) disease, is an important and widespread pathogen of barley. This study aimed to quantify and characterize the virulence of Ptt isolates collected from experimental fields of barley in Hungary. Infection responses across 20 barley differentials were obtained from seedling assays of 34 Ptt isolates collected from three Hungarian breeding stations between 2008 and 2018. Twenty-eight Ptt pathotypes were identified. Correspondence analysis followed by hierarchical clustering on the principal components and host-by-pathogen GGE biplots suggested a continuous range of virulence and an absence of specific isolate × barley differential interactions. The isolates were classified into four isolate groups (IG) using agglomerative hierarchical clustering. One IG could be distinguished from other IGs based on avirulence/virulence on one to five barley differentials. Several barley differentials expressed strong resistance against multiple Ptt isolates and may be useful in the development of NFNB-resistant barley cultivars in Hungary. Our results emphasize that the previously developed international barley differential set needs to be improved and adapted to the Hungarian Ptt population. This is the first report on the pathogenic variations of Ptt in Hungary.

Genome-Wide Comprehensive Identification and In Silico Characterization of Lectin Receptor-Like Kinase Gene Family in Barley (Hordeum vulgare L.).

Lectin receptor-like kinases (LecRLKs) are a significant subgroup of the receptor-like kinases (RLKs) protein family. They play crucial roles in plant growth, development, immune responses, signal transduction, and stress tolerance. However, the genome-wide identification and characterization of LecRLK genes and their regulatory elements have not been explored in a major cereal crop, barley (Hordeum vulgare L.). Therefore, in this study, integrated bioinformatics tools were used to identify and characterize the LecRLK gene family in barley. Based on the phylogenetic tree and domain organization, a total of 113 LecRLK genes were identified in the barley genome (referred to as HvlecRLK) corresponding to the LecRLK genes of Arabidopsis thaliana. These putative HvlecRLK genes were classified into three groups: 62 G-type LecRLKs, 1 C-type LecRLK, and 50 L-type LecRLKs. They were unevenly distributed across eight chromosomes, including one unknown chromosome, and were predominantly located in the plasma membrane (G-type HvlecRLK (96.8%), C-type HvlecRLK (100%), and L-type HvlecRLK (98%)). An analysis of motif composition and exon-intron configuration revealed remarkable homogeneity with the members of AtlecRLK. Notably, most of the HvlecRLKs (27 G-type, 43 L-type) have no intron, suggesting their rapid functionality. The Ka/Ks and syntenic analysis demonstrated that HvlecRLK gene pairs evolved through purifying selection and gene duplication was the major factor for the expansion of the HvlecRLK gene family. Exploration of gene ontology (GO) enrichment indicated that the identified HvlecRLK genes are associated with various cellular processes, metabolic pathways, defense mechanisms, kinase activity, catalytic activity, ion binding, and other essential pathways. The regulatory network analysis identified 29 transcription factor families (TFFs), with seven major TFFs including bZIP, C2H2, ERF, MIKC_MADS, MYB, NAC, and WRKY participating in the regulation of HvlecRLK gene functions. Most notably, eight TFFs were found to be linked to the promoter region of both L-type HvleckRLK64 and HvleckRLK86. The promoter cis-acting regulatory element (CARE) analysis of barley identified a total of 75 CARE motifs responsive to light responsiveness (LR), tissue-specific (TS), hormone responsiveness (HR), and stress responsiveness (SR). The maximum number of CAREs was identified in HvleckRLK11 (25 for LR), HvleckRLK69 (17 for TS), and HvleckRLK80 (12 for HR). Additionally, HvleckRLK14, HvleckRLK16, HvleckRLK33, HvleckRLK50, HvleckRLK52, HvleckRLK56, and HvleckRLK110 were predicted to exhibit higher responses in stress conditions. In addition, 46 putative miRNAs were predicted to target 81 HvlecRLK genes and HvlecRLK13 was the most targeted gene by 8 different miRNAs. Protein-protein interaction analysis demonstrated higher functional similarities of 63 HvlecRLKs with 7 Arabidopsis STRING proteins. Our overall findings provide valuable information on the LecRLK gene family which might pave the way to advanced research on the functional mechanism of the candidate genes as well as to develop new barley cultivars in breeding programs.

Molecular Identification and Characterization of Hevein Antimicrobial Peptide Genes in Two-Row and Six-Row Cultivars of Barley (Hordeum vulgare L.).

Heveins are one of the most important groups of plant antimicrobial peptides. So far, various roles in plant growth and development and in response to biotic and abiotic stresses have reported for heveins. The present study aimed to identify and characterize the hevein genes in two-row and six-row cultivars of barley. In total, thirteen hevein genes were identified in the genome of two-row and six-row cultivars of barley. The identified heveins were identical in two-row and six-row cultivars of barley and showed a high similarity with heveins from other plant species. The hevein coding sequences produced open reading frames (ORFs) ranged from 342 to 1002bp. Most of the identified hevein genes were intronless, and the others had only one intron. The hevein ORFs produced proteins ranged from 113 to 333 amino acids. Search for conserved functional domains showed CBD and LYZ domains in barley heveins. All barley heveins comprised extracellular signal peptides ranged from 19 to 35 amino acids. The phylogenetic analysis divided barley heveins into two groups. The promoter analysis showed regulatory elements with different frequencies between two-row and six-row cultivars. These cis-acting elements included elements related to growth and development, hormone response, and environmental stresses. The expression analysis showed high expression level of heveins in root and reproductive organs of both two-row and six-row cultivars. The expression analysis also showed that barley heveins is induced by both biotic and abiotic stresses. The results of antimicrobial activity prediction showed the highest antimicrobial activity in CBD domain of barley heveins. The findings of the current study can improve our knowledge about the role of hevein genes in plant and can be used for future studies.

Managing nitrogen to promote quality and profitability of North Dakota two‐row malting barley

AbstractAs the demand and cultivation of two‐row malting barley (Hordeum vulgare L.) increases in the Northern Great Plains, updated nitrogen (N) recommendations are increasingly necessary. Not only does N play a role in grain yield, but it also impacts grain malting characteristics, including protein and kernel plump. To determine the impacts N rate and availability have on two‐row malting barley, two experimental sites were established in eastern North Dakota during the 2020 and 2021 growing seasons. Treatments consisted of five fertilizer rates from 0 to 180 kg N ha−1 and two malting barley cultivars. Soil samples to be analyzed for nitrate‐N were taken prior to planting and N credit estimates from the previous crop were considered to determine the total known available nitrogen (TKAN) in the soil. It was determined there was a strong relationship between N rate and grain yield along with a strong positive correlation between N rate and grain protein. No significant interactions between N rate and kernel plump were noted. When the relationship between relative grain yield and TKAN was modeled using a best‐fit regression, maximum yield was attained at 210 kg TKAN ha−1 with a grain protein of 128 g kg−1, meeting malting quality requirements. When factoring in grain value and cost of urea fertilizer, the TKAN range needed to produce the crop at the highest profitability was lower than TKAN of maximum yield, ranging from 89 to 190 kg TKAN ha−1.

Genetic evaluation of hulless barley (Hordeum vulgare) genotypes for morpho-physiological traits and stripe rust resistance

Nowadays, hulless barley (Hordeum vulgare L.) is gaining importance as a raw material for food industry due to its highly nutritive value. Non-availability of high yield and disease resistant varieties has restricted its production in marginal areas over the last two decades. Therefore, an experiment was conducted during 2017–18 and 2018–19 at the Seed and Research Farm of ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana to characterized 11 exotic hulless barley genotypes and 4 Indian barley cultivars for morpho-physiological characters and stripe rust resistance. Seedling resistant test for stripe rust was done at Regional Station (ICAR-Indian Institute of Wheat and Barley Research), Flowerdale, Shimla, Himachal Pradesh. Highly significant genotypic differences were found for morpho-physiological traits during analysis of variance. Genotype DWRNB28 showed a good combination of desirable traits, viz. early maturity (118 days), dwarf plant height (64.4 cm) and high 1000-grain weight (55.2 g). Maximum number of tillers per meter (149) was observed in DWRNB17. Genotype DWRNB25 was found resistant to maximum pathotypes (6S0, 7S0, and Q) at the seedling stage. DWRNB23 and DWRNB25 with pooled ACI=0 and ACI=0.04 respectively, were found immune to stripe rust in field conditions. These promising genotypes can be utilized as donor parents for the development of high-yielding and stripe-rust-resistant hulless barley varieties.

Evaluating the Impact of Plant Growth on Different Varieties of Wheat and Barley Treated With Biofertilizers

Purpose: Biofertilizers have gained recognition as a crucial element in integrated plant nutrient management, contributing to sustainable agriculture. Biofertilizers are natural products that contain living microorganisms capable of enhancing plant growth and improving soil fertility. This study aims to assess the impact of different biofertilizers on the growth of various varieties of wheat and barley. The primary objective of this research is to evaluate the efficacy of biofertilizers as an alternative to chemical fertilizers for promoting the growth of wheat and barley crops.&#x0D; Methodology: The experiment involves treating multiple cultivars of wheat and barley with biofertilizers, while a control group is administered traditional chemical fertilizers for comparison. The growth parameters, including plant height, leaf area, root development, and yield, are carefully monitored and recorded throughout the experiment. The study is conducted under controlled environmental conditions to ensure consistency across all plant samples. Collected data is subjected to statistical analysis to determine any significant disparities in growth between the biofertilizer-treated group and the control group.&#x0D; Findings: The utilization of biofertilizers resulted in remarkable growth improvements in the plants as opposed to conventional fertilizers. The plants treated with biofertilizers exhibited noticeable enhancements in various parameters, including increased plant height, expanded leaf area, elongated root length, and greater biomass accumulation. Among the different crop varieties, certain ones showcased a more pronounced response to biofertilizer treatments. Specifically, varieties W1 and W2 of wheat displayed the highest levels of growth enhancement when treated with biofertilizers, while barley varieties B1 and B2 showed significant improvements as well. Furthermore, the application of biofertilizers led to higher grain weight and increased yield per unit area in the crops compared to traditional fertilizers. Notably, the biofertilizer-treated crops also exhibited notable enhancements in quality attributes, such as improved nutritional content and reduced pesticide residues.&#x0D; Unique contributor to theory, policy and practice: The utilization of biofertilizers demonstrated significant advantages over traditional fertilizers, resulting in improved plant growth, increased yield, and enhanced quality attributes in various crop varieties.

Genome-Wide Identification of Ascorbate Peroxidase Gene Family in Two Contrasting Barley (Hordeum vulgare L.) Cultivars in Response to Abiotic Stresses

Genome-Wide Identification of Ascorbate Peroxidase Gene Family in Two Contrasting Barley (Hordeum vulgare L.) Cultivars in Response to Abiotic Stresses

Toxic effect of elements on the germination and initial development of barley seeds (Hordeum vulgare L.)

Barley (Hordeum vulgare) is an agricultural vegetable from the Poaceae family used in food and beer production. The study aimed to evaluate the toxic effect of Aluminum (Al) and Copper (Cu) on germination and initial development in barley seeds cultivar KWS Irina. Different concentrations (0, 35, 85 and 125 mg L-1) of aqueous solution of Al and Cu were produced from their chlorides. The toxicity experiment was carried out in a germination box maintained in a germination chamber with a 12-h photoperiod. After 15 days of germination, the seedlings were measured using a millimetric ruler (cm) where they were evaluated for plant length, root length, and fresh and dry mass of plant and root determined on a digital analytical scale (g). Barley seedlings cultivar KWS Irina demonstrated to be intolerant to concentrations of the toxic elements Al and Cu in all plant parameters analyzed, except for plant dry mass. Future studies should be carried out comparing the initial and reproductive development of this barley cultivar in terms of the presence and absence of toxic elements.

Hordeum vulgareCYP76M57 catalyzes C2 shortening of tryptophan side chain by C-N bond rearrangement in gramine biosynthesis.

The indole alkaloid gramine, 3-(dimethylaminomethyl)indole, is a defensive specialized metabolite found in some barley cultivars. In its biosynthetic process, the tryptophan (Trp) side chain is shortened by two carbon atoms to produce 3-(aminomethyl)indole (AMI), which is then methylated by N-methyltransferase (HvNMT) to produce gramine. Although side chain shortening is one of the crucial scaffold formation steps of alkaloids originating from aromatic amino acids, the gene and enzyme involved in the Trp-AMI conversion reactions are unknown. In this study, through RNA-seq analysis, 35 transcripts were shown to correlate with gramine production; among them, an uncharacterized cytochrome P450 (CYP) gene, CYP76M57, and HvNMT were identified as candidate genes for gramine production. Transgenic Arabidopsis thaliana and rice overexpressing CYP and HvNMT accumulate AMI, N-methyl-AMI, and gramine. CYP76M57, heterologously expressed in Pichia pastoris, was able to act on Trp to produce AMI. Furthermore, the amino group nitrogen of Trp was retained during the CYP76M57-catalyzed reaction, indicating that the C2 shortening of Trp proceeds with an unprecedented biosynthetic process, the removal of the carboxyl group and Cα and the rearrangement of the nitrogen atom to Cβ. In some gramine-non-accumulating barley cultivars, arginine 104 in CYP76M57 is replaced by threonine, which abolished the catalytic activity of CYP76M57 to convert Trp into AMI. These results uncovered the missing committed enzyme of gramine biosynthesis in barley and contribute to the elucidation of the potential functions of CYPs in plants and undiscovered specialized pathways.

Heat Pre-Treatment Modified Host and Non-Host Interactions of Powdery Mildew with Barley Brassinosteroid Mutants and Wild Types.

High temperatures associated with climate change may increase the severity of plant diseases. This study investigated the effect of heat shock treatment on host and non-host barley powdery mildew interactions using brassinosteroid (BR) mutants of barley. Brassinosteroids are plant steroid hormones, but so far little is known about their role in plant-fungal interactions. Wild type barley cultivar Bowman and its near-isogenic lines with disturbances in BR biosynthesis or signalling showed high compatibility to barley powdery mildew race A6, while cultivar Delisa and its BR-deficient mutants 522DK and 527DK were fully incompatible with this pathogen (host plant-pathogen interactions). On the other hand, Bowman and its mutants were highly resistant to wheat powdery mildew, representing non-host plant-pathogen interactions. Heat pre-treatment induced shifts in these plant-pathogen interactions towards higher susceptibility. In agreement with the more severe disease symptoms, light microscopy showed a decrease in papillae formation and hypersensitive response, characteristic of incompatible interactions, when heat pre-treatment was applied. Mutant 527DK, but not 522DK, maintained high resistance to barley powdery mildew race A6 despite heat pre-treatment. By 10 days after heat treatment and infection, a noticeable shift became apparent in the chlorophyll a fluorescence and in various leaf reflectance parameters at all genotypes.

Agrobacterium-mediated transfer of the Fusarium graminearum Tri6 gene into barley using mature seed-derived shoot tips as explants

Key messageWe transferred the Tri6 gene into the elite barley GemCraft via new transformation method through shoot organogenesis and identified the rearrangements of transgenes and phenotypic variations in the transgenic plants.Despite its agronomic and economic importance, barley transformation is still very challenging for many elite varieties. In this study, we used direct shoot organogenesis to transform the elite barley cultivar GemCraft with the RNAi constructs containing Tri6 gene of Fusarium graminearum, which causes fusarium head blight (FHB). We isolated 4432 shoot tips and co-cultured these explants with Agrobacterium tumefaciens. A total of 25 independent T0 transgenic plants were generated including 15 events for which transgene-specific PCR amplicons were observed. To further determine the presence of transgenes, the T1 progenies of all 15 T0 plants were analyzed, and the expected PCR products were obtained in 10 T1 lines. Droplet digital (dd) PCR analysis revealed various copy numbers of transgenes in the transgenic plants. We determined the insertion site of transgenes using long-read sequencing data and observed the rearrangements of transgenes. We found phenotypic variations in both T1 and T2 generation plants. FHB disease was evaluated under growth chamber conditions, but no significant differences in disease severity or deoxynivalenol accumulation were observed between two Tri6 transgenic lines and the wildtype. Our results demonstrate the feasibility of the shoot tip transformation and may open the door for applying this system for genetic improvement and gene function research in other barley genotypes.