Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties and canopy structure of wheat change across different growth stages, leading to variations in spectral responses that significantly impact the estimation of wheat LNC. This study aims to investigate the construction of feature combination indices (FCIs) sensitive to LNC across multiple wheat growth stages, using remote sensing data to develop an LNC estimation model that is suitable for multiple growth stages. The research employs UAV multispectral remote sensing technology to acquire canopy imagery of wheat during the early (Jointing stage and Booting stage) and late (Early filling and Late filling stages) in 2021 and 2022, extracting spectral band reflectance and texture metrics. Initially, twelve sensitive spectral feature combination indices (SFCIs) were constructed using spectral band information. Subsequently, sensitive texture feature combination indices (TFCIs) were created using texture metrics as an alternative to spectral bands. Machine learning algorithms, including partial least squares regression (PLSR), random forest regression (RFR), support vector regression (SVR), and Gaussian process regression (GPR), were used to integrate spectral and texture information, enhancing the estimation performance of wheat LNC across growth stages. Results show that the combination of Red, Red edge, and Near-infrared bands, along with texture metrics such as Mean, Correlation, Contrast, and Dissimilarity, has significant potential for LNC estimation. The constructed SFCIs and TFCIs both enhanced the responsiveness to LNC across multiple growth stages. Additionally, a sensitive index, the Modified Vegetation Index (MVI), demonstrated significant improvement over NDVI, correcting the over-saturation concerns of NDVI in time-series analysis and displaying outstanding potential for LNC estimation. Spectral information outperforms texture information in estimation capability, and their integration, particularly with SVR, achieves the highest precision (coefficient of determination (R2) = 0.786, root mean square error (RMSE) = 0.589%, and relative prediction deviation (RPD) = 2.162). In conclusion, the sensitive FCIs developed in this study improve LNC estimation performance across multiple growth stages, enabling precise monitoring of wheat LNC. This research provides insights and technical support for the construction of sensitive indices and the precise management of nitrogen nutrition status in field crops.