Banana Production Research Articles

A socioeconomic and cost benefit analysis of Tropical Race 4 (TR4) prevention methods among banana producers in Colombia.

The global banana industry faces a significant threat from Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4). While prior research has concentrated on TR4's dissemination, reproductive conditions, and resistant banana varieties, this study employs a socioeconomic and cost-benefit analysis to explore the vulnerability of banana producers to TR4 in Colombia. It assesses the financial viability of current monitoring strategies and estimates potential losses in the event of TR4 spreading within the study area. Interviews were conducted with producers and key stakeholders in Colombia's top two banana-producing departments, Antioquia and Magdalena. The findings reveal that farming systems are highly vulnerable to TR4, particularly due to the prevalent use of corms. Producers employ preventive measures such as cement paths, fences, disinfecting stations, and footbaths to counteract TR4's spread. A cost-benefit analysis indicates that the benefits of these prevention methods significantly outweigh the associated costs, with a net present value of implementing prevention strategies per hectare of $95,389 USD and $112,527 USD in Magdalena and Antioquia and a benefit-cost ratio of 3.1 and 4.2, respectively. Considering the substantial impact TR4 could have in Colombia if it becomes more widespread, we recommend widespread adoption of preventive measures, including the construction and utilization of cement paths and disinfectant methods on all banana farms. Additionally, to enhance awareness and early detection, we propose leveraging technology, such as mobile applications (apps) and chat groups, to empower farmers in identifying and preventing the spread of TR4.

The Effect of Some Biological Fungicides and Liquid Organic Fertilizers on Sigatoka Disease on ‘Mas’ Banana Plants (Musa acuminata Colla)

Banana (Musa acuminata Colla) is a tropical fruit with great economic potential and export prospects. According to the Directorate General of Horticulture, Ministry of Agriculture, banana production from 2009 to 2014 fluctuated due to attacks by Plant Destruction Organisms (PDO), particularly Sigatoka disease caused by the fungus Mycosphaerella musciola. This study investigated biological agents like Trichoderma sp. and Gliocladium sp., along with liquid organic fertilizers, to control the disease without synthetic fungicides.The research, conducted at the University of Muhammadiyah Malang, used a split-plot design with two factors: biological pesticides (Tricogreen, Natural Glio, Trico-G) and liquid organic fertilizers (rabbit urine POC, shrimp shell MOL, banana hump MOL). The experiment had nine treatment combinations with three plant samples each. Results showed that biological pesticides significantly reduced Sigatoka disease intensity, with the J3P3 treatment (Trico-G and Banana Hump MOL) achieving the lowest infection rate of 29.55%, reducing disease intensity by 70.45%. Liquid organic fertilizers also improved plant growth, increasing leaf number and leaf area. This indicates that biological agents and organic fertilizers effectively manage Sigatoka disease and promote healthy banana plant growth.

Calcium-Binding Protein and Polymorphism in Musa spp. Somaclones Resistant to Fusarium oxysporum

The fresh fruits of ‘Grande Naine’ (Cavendish AAA—Musa spp.) dominate the world market, especially in countries with a population in a situation of social vulnerability. However, Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense race 4 Subtropical (Foc ST4), emerges as a serious threat to banana production, requiring the development of resistant cultivars based on biotechnological strategies, such as the induction of mutation in tissue culture. This study aimed to identify and characterize genetic variation in somaclones resistant to Fusarium oxysporum f.sp. cubense subtropical race 4 (Foc ST4), derived from ‘Grand Naine’ bananas, by molecular markers based on retrotransposons IRAP (Inter-retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism). Nine combinations of IRAP and six combinations of REMAP primers were used. The low number of polymorphic bands did not allow for genetic diversity studies; however, ten polymorphic bands between the somaclones and control were sequenced. Of these, three presented good base calling and were aligned, namely, 1AF, 2AF, and 3AF bands. Only the 1AF band presented function related to stress response with homology to a calcium-binding protein. These proteins act early in plant infection as secondary messengers activated by pathogen-associated molecular patterns (PAMPs), initiating the cascade of plant defense signals. The fact that this band is present in all somaclones reinforces previous assessments of their resistance to Foc ST4. The use of markers IRAP and REMAP produced polymorphic bands that can, through future primer design and field validations, accelerate the identification of resistant banana genotypes for use in banana genetic breeding programs.

Agroecological Aptitude of the Northeast of Formosa (Argentinean Subtropical Region) for Banana Production Assessed by Multiple Factor Analysis

Banana (Musa spp.) is an important crop in the economies of many developing countries. In the north of Argentina, a subtropical region, banana plants grow in a suboptimal environment that limits yield because only one harvest per year is achieved. The objective of this work was to characterize the agroecological aptitude of Formosa, Argentina, for banana production through the behavior of three varieties of international use: Williams, Jaffa and Grand Naine, evaluated over five consecutive years. The three-way data analysis technique called Multiple Factor Analysis (MFA) was used for evaluating the varieties’ performances across cycles of production. The results allowed for inferring the existence of a genotype x environment interaction (GEI), corroborated by two-way factorial ANOVA. In order to determine how this suboptimal environment affected the development of each genotype of this perennial crop, Dual Multiple Factor Analysis (DMFA) was applied to jointly analyze the correlation structure between the traits that contributed to the performance of each variety in each year. The correlation structures between variables were different in each population and varied between years. All traits showed great variation between the years and genotypes, with the fruit peel thickness being the most discrepant throughout the years. However, Formosa appeared as a promising subtropical agroecological environment to produce banana because the varieties’ performances were acceptable for large-scale production systems. In addition to evaluating the adequate aptitude for cultivating banana in Formosa considering the significant effect of the GEIs, this research made a methodological contribution by proposing the use of three-way data analysis in Agronomy Science via MFA and DMFA.

High removal of volatile organic compounds on hierarchical carbons prepared from agro-industrial waste of banana fruit production for air decontamination.

Activated carbons were prepared from residues from agro-industrial banana production (banana pseudostem) and evaluated in the capture of five different volatile organic compounds (VOCs): dichloromethane, chloroform, ethyl acetate, hexane, and cyclohexane. The biomass was first submitted to a hydrothermal treatment in the presence of KOH or ZnCl2 as activating agents, followed by a dry pyrolysis. This new advance in methodology contributes to producing activated carbons with hierarchical porosity and high surface areas (701-1312 m2g-1), promoting increased interest in managing waste from banana fruit agricultural production. VOC capture studies were performed by thermal analysis, and capture capacities were similar to or higher than those presented in the literature. Higher adsorption capacities were related to the amount of available micropores, and the capture capacity was enhanced by the contribution of small mesopores. As the highest adsorbed amounts of dichloromethane (933mgg-1 at 25°C) were obtained for the material activated with ZnCl2 (1:3), further studies were carried out for this system. The experimental data was fitted using a pseudo-first-order kinetic model. A study was carried out in different atmospheres (He, N2, air), showing that co-adsorption is occurring. Under simulated environmental conditions, the capture capacity decreased slightly at equilibrium, and the new adsorbent was used for up to ten cycles without significantly losing its efficiency, indicating good application in the field.

Socio-Economic Impacts of Fruit Crop Production in the Mungo Corridor, Littoral Region, Cameroon

Purpose: Agriculture, particularly fruit crop production, serves as a vital driver of economic growth worldwide. In Cameroon, the Mungo Corridor plays a substantial role in the agricultural sector, particularly in fruit crop production. This study aims to assess the socio-economic impacts of fruit crop production within the Mungo Corridor, a region known for cultivating bananas, pineapples, and mangoes. By evaluating the economic contributions and challenges, the study seeks to provide insights for policies that could enhance the sustainability and development of fruit production in the area. The study adopted a multi-stage sampling technique to select respondents and resource persons. Materials and Methods: Data collection involved the use of structured and pre-tested questionnaires along with an interview guide. Quantitative data was analyzed using the Statistical Package for Social Sciences (SPSS) version 16.0 and Microsoft Excel, while qualitative data from interviews was subjected to thematic analysis. Six subdivisions from the Mungo Division were purposely chosen to represent the agricultural activities within the region. Findings: The study confirms that fruit crop production has significant socio-economic benefits in the Mungo Corridor. Over a 10-year period, banana production reached 195,000 tons, while pineapple production rose from 6,543 tons in 2012 to 28,141 tons in 2022. Papaya production increased from 36 tons to 86 tons during the same period. The income from fruit sales enabled stakeholders to develop socio-economic activities, fostering regional development. Fruit crop production contributed to the construction of 12 schools (primary and secondary), 21 health centers, the rehabilitation of 8 fruit markets, 3 bus stations, and growth in the informal sector. However, the study also identified several challenges with Farmers face limited access to markets. Poor infrastructure affects production and distribution. The looming threat of climate change presents long-term risks to fruit farming. Implications to Theory, Practice and Policy: To address the challenges and ensure sustainable fruit crop production in the Mungo Corridor, the study recommends the implementation of policies that promote local products and support farmers through market access initiatives. Invest in transport, storage, and market infrastructure to improve efficiency. Develop interventions to mitigate the impact of climate change on fruit production and strengthen cooperation among farmers, government, and private actors to maximize the benefits of fruit crop production and foster sustainable development. By highlighting the essential role of fruit farming in the socio-economic development of the Mungo Corridor, the study emphasizes the need for renewed efforts to support the sector and enhance its contributions to regional growth.

Aerial Spraying and Its Impacts on Human Health in Banana-Growing Areas of Ecuador.

The present work examines the relationship between aerial spraying and its health impacts on the population living in the banana production areas of Ecuador (the rural sectors of the cantons Milagro and Naranjito, Guayas Province). Objectives: the objectives of this study are to obtain information on sanitation, basic services, and environmental rationality and to interpret the low levels of cholinesterase and prevalent diseases among the population. Methods: the methodology involved a face-to-face questionnaire, the formal authorization of an informed consent document, and venipuncture for cholinesterase tests. The information was processed in the EPI-INFO system 7.2 (statistical software for professionals and researchers dedicated to public health), with the certification of protocols issued by the Bioethics Committee of the Kennedy Hospital Clinic of Ecuador. Results: the results showed that 89.5% of inhabitants do not have access to drinking water, 92.5% do not have a sewage disposal service, 97.50% experience aerial spraying at their homes or workplaces, and 57% have low cholinesterase levels. Additionally, several gastrointestinal, respiratory, neurological, dermatological, and reproductive disorders were detected among the inhabitants. Conclusions: we found that companies in the banana sector have not implemented corporate social responsibility measures. For example, no blood tests are conducted to monitor cholinesterase levels or to track hereditary disorders. Moreover, entities such as the Ministry of Public Health have not taken action to serve this at-risk population.

Using CRISPR-Cas9 genome editing to enhance disease resistance in banana

Abstract Bananas and plantains ( Musa spp.) are leading global fruit crops, cultivated in over 150 countries and several island nations and serving as fruit and staple food in tropical and subtropical regions. Notwithstanding their economic importance, banana production is hindered by pests, diseases, and climatic challenges, resulting in significant yield gaps and post-harvest losses. Conventional breeding methods are time-consuming and labor-intensive due to the 15–18 months long life cycle and seedless nature of the edible crop, while transgenic approaches face regulatory, public acceptance, and sociopolitical hurdles. The advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas9) technology offers a precise, targeted, and efficient tool for genetic mutation derived from the bacterial antiviral defense system. Researchers are exploring CRISPR-Cas9 to develop banana varieties resistant to diseases and pests, exhibit higher yields, and possess enhanced nutritional profiles. The well-annotated complete banana genome, combined with advanced regeneration and transformation systems, facilitates the application of CRISPR-Cas9 technology. This innovative approach promises to create superior banana cultivars that benefit consumers and farmers, addressing critical challenges in banana production and contributing to global food security. This review article discusses the challenges in banana production, including biotic and abiotic constraints, and explores recent advancements in genome editing.

Physical, functional and bioactive properties of microencapsulated powders from banana pseudostem and inflorescence extracts

Application of agricultural by-products in the functional food and beverage industry is currently gaining prominence. Banana (Musa spp) is a popular tropical fruit with global production of 124.97 million tonnes. The banana production industry contributes to large amount of solid waste/ banana by-products, such as, pseudostem and inflorescence. Palayankodan (Musa × paradisiaca Mysore AAB group), Nendran (Musa × paradisiaca AAB group) and Njalipoovan (Musa × paradisiaca AB group) are three popular and common cultivars in Kerala, a state in South India. The present study was aimed to extract the potential bioactive compounds from the pseudostem and inflorescence of the above-mentioned cultivars and to standardise the process of microencapsulation using spray drying. Ultrasonication assisted extraction using ethanol as solvent was carried out. The extract and wall material parameters were standardised for microencapsulation. The encapsulated powders were analysed for encapsulation yield, retention efficiency, physical properties, phytochemical composition, antioxidant potential and anti-diabetic activity. The encapsulation yields and retention efficiencies of the encapsulated powders were found to be approximately 75 and 70.51%, respectively. Results revealed that the powders exhibited lower bulk density, good solubility and reconstitutability. Scanning electron microscopy was also conducted to reveal the particle morphology. All the powders exhibited smooth, spherical shape, with no pores. The phenolic and flavonoid contents of the encapsulated pseudostem and inflorescence extract powders ranged from 2.75 to 3.13 mg GAE/ g of powder and 34.83 to 46.67 mg QE/g of powder, respectively. The present study also reported the in vitro bioactive properties, in terms of antioxidant and anti-diabetic activities of the encapsulated powders. The HPLC analysis of the microencapsulated powders revealed the presence of gallic acid, protocatechuic acid, trans cinnamic acid, trans ferulic acid, epicatechin and syringic acid. It is clear from the study that the encapsulated powders from banana by-products have a great potential to be utilised by the functional food industry.Graphical

Exploring Nonanol from Bacillus velezensis (YEBBR6) for Root-Knot Nematode Management in Bananas: Integrating Experimental and Computational Approaches

Nematode infestations pose a severe threat to global banana production, leading to substantial yield losses and economic damage. Among these, the root-knot nematode Meloidogyne incognita is particularly detrimental, causing root galls that severely impair water and nutrient uptake, leading to stunted growth, reduced fruit quality, and significant economic losses for farmers. In the face of increasing agricultural challenges, bacterial endophytes have emerged as essential tools for sustainable agriculture, offering ecofriendly solutions to enhance plant health and manage pests. The present study aims to identify and characterise biomolecules from bacterial endophyte Bacillus velezensis (YEBBR6) with potential nematicidal properties against root-knot nematode Meloidogyne incognita infecting banana. The analysis of volatile and non-volatile organic compounds (VOCs/NVOCs) from the inhibition zone during the ditrophic interaction between B. velezensis (YEBBR6) and Fusarium oxysporum f.sp. cubense (Foc) revealed the presence of several distinctive biomolecules with antifungal and antimicrobial properties. These compounds included linoelaedic acid, nonanol, acetylvaleryl, 5–hydroxyl methyl furfural, clindamycin, allobarbital, 3-thiazolidine carboxamide, azulene, aminomorpholine, procyclidine, campholic acid, 3 amino-4 hydroxy phenyl sulfone, 3–deoxy mannoic lactone, hexadecanoic acid, oleic acid, and dihydroacridine. The in-silico analysis revealed that nonanol, exhibited strong binding efficacy against M. incognita protein targets, including cytochrome C oxidase subunit 1, calreticulin, neuropeptide G-protein coupled receptor, chorismate mutase 1, venom allergen-like proteins, and β-1,4-endoglucanase. Notably, nonanol proved more effective than the commercially used nematicide carbofuran 3G. Molecular dynamics studies further supported the potential of nonanol, and its nematicidal properties were validated through in vitro and pot culture studies. Moreover, nonanol was found to induce the expression of defense-related genes, such as MAPK10, WRKY22, ascorbate peroxidase, catalase, and NADPH oxidase, in banana seedlings, thus enhancing the plant's immune response. These findings highlight the potential of nonanol, derived from B. velezensis (YEBBR6), as a potent and environmentally friendly alternative for controlling nematode infestations in banana cultivation.

Optimizing banana production in aroma Lumajang business using goal programming method and sensitivity analysis

Optimizing banana production in aroma Lumajang business using goal programming method and sensitivity analysis

Soil Fertility Changes and Crop Productivity of Banana by Continuous Adoption of Banana-Cowpea Intercropping with Nutrient Management Practices

A front line demonstration was conducted in farmer’s field in an area of 8 ha with Poovan Banana variety taken as test crop. Banana is being cultivated to an extent of 2000 ha in six major banana growing blocks in the Namakkal district, Tamil Nadu. Indiscriminate use of chemical fertilizers and mono cropping of banana leads to widespread deficiency in available nutrients (OC-62%, N -67%, Zn-98%, B-58%). This leads to tremendous yield loss. Hence this study was conducted in randomized block design. From this study, it can be concluded that growing of cowpea as intercrop in banana along with application of integrated nutrient management practice (demo plot) observed higher available nutrient status than farmers practice. Among the all soil parameters, a noticeable increase was 29 kg in available nitrogen and 0.30% in organic carbon observed in soil after incorporation of cowpea along with recommended dose of nutrient application at timely interval. Growth and yield attributing characteristics also was recorded higher in demo plot than farmers practice (check). Highest banana yield of 34.80 t ha-1 was recorded in the demo plot (KVK intervention from sowing to harvest) whereas farmers practice (check) recorded 30.2 t ha-1. The demo yield proved its superiority by recording 15.07 % yield increase over check and having higher net return of Rs.1,89,543 with BC ratio 2.30 than check.

Assessment of Dataset Scalability for Classification of Black Sigatoka in Banana Crops Using UAV-Based Multispectral Images and Deep Learning Techniques

This paper presents an evaluation of different convolutional neural network (CNN) architectures using false-colour images obtained by multispectral sensors on drones for the detection of Black Sigatoka in banana crops. The objective is to use drones to improve the accuracy and efficiency of Black Sigatoka detection to reduce its impact on banana production and improve the sustainable management of banana crops, one of the most produced, traded, and important fruits for food security consumed worldwide. This study aims to improve the precision and accuracy in analysing the images and detecting the presence of the disease using deep learning algorithms. Moreover, we are using drones, multispectral images, and different CNNs, supported by transfer learning, to enhance and scale up the current approach using RGB images obtained by conventional cameras and even smartphone cameras, available in open datasets. The innovation of this study, compared to existing technologies for disease detection in crops, lies in the advantages offered by using drones for image acquisition of crops, in this case, constructing and testing our own datasets, which allows us to save time and resources in the identification of crop diseases in a highly scalable manner. The CNNs used are a type of artificial neural network widely utilised for machine training; they contain several specialised layers interconnected with each other in which the initial layers can detect lines and curves, and gradually become specialised until reaching deeper layers that recognise complex shapes. We use multispectral sensors to create false-colour images around the red colour spectra to distinguish infected leaves. Relevant results of this study include the construction of a dataset with 505 original drone images. By subdividing and converting them into false-colour images using the UAV’s multispectral sensors, we obtained 2706 objects of diseased leaves, 3102 objects of healthy leaves, and an additional 1192 objects of non-leaves to train classification algorithms. Additionally, 3640 labels of Black Sigatoka were generated by phytopathology experts, ideal for training algorithms to detect this disease in banana crops. In classification, we achieved a performance of 86.5% using false-colour images with red, red edge, and near-infrared composition through MobileNetV2 for three classes (healthy leaves, diseased leaves, and non-leaf extras). We obtained better results in identifying Black Sigatoka disease in banana crops using the classification approach with MobileNetV2 as well as our own datasets.

Unveiling the Full Protein Effectorome of the Black Sigatoka Pathogen Pseudocercospora fijiensis—An In Silico Approach

Pseudocercospora (previously Mycosphaerella) fijiensis is a hemibiotroph fungus and the causal agent of black Sigatoka disease, one of the most significant threats to banana production worldwide. Only a few genomics reports have paid any attention to effector proteins, which are key players in pathogenicity. These reports focus on canonical effectors: small secreted proteins, rich in cysteines, containing a signal peptide and no transmembrane domain. Thus, bias in previous reports has resulted in the non-canonical effectors being, in effect, excluded from the discussion of effectors in P. fijiensis pathogenicity. Here, using WideEffHunter and EffHunter, bioinformatic tools which identify non-canonical and canonical effectors, respectively, we predict, for the first time, the full effectorome of P. fijiensis. This complete effectorome comprises 5179 proteins: 240 canonical and 4939 non-canonical effectors. Protein families related to key functions of the hemibiotrophic lifestyle, such as Salicylate hydroxylase and Isochorismatase, are widely represented families of effectors in the P. fijiensis genome. An analysis of the gene distribution in core and dispensable scaffolds of both classes of effectors revealed a novel genomic structure of the effectorome. The majority of the effectors (canonical and non-canonical) were found to be harbored in the core scaffolds, while dispensable scaffolds harbored less than 10% of the effectors, all of which were non-canonical. Additionally, we found the motifs RXLR, YFWxC, LysM, EAR, [Li]xAR, PDI, CRN, and ToxA in the effectors of P. fijiensis. This novel genomic structure of effectors (more enriched in the core than in the dispensable genome), as well as the occurrence of effector motifs which were also observed in four other fungi, evidences that these phenomena are not unique to P. fijiensis; rather, they are widely occurring characteristics of effectors in other fungi.

Tissue Culture in Banana Cultivation: A Review of its Impact on Disease Management, Yield Improvement, and Sustainable Production

In several tropical and subtropical areas, bananas (Musa spp.) are an important food source and commercial crop. Disease transmission and production uncertainty are common problems with traditional propagation techniques such as suckers. By supplying consistent, high-quality, and disease-free planting material, tissue culture, known as micro propagation, represents a potential alternative. The application of tissue culture has greatly enhanced disease management in banana cultivation. Reducing the incidence of ailments like Fusarium wilt, bacterial wilt, and Banana Bunchy Top Virus (BBTV), pathogen-free plantlets are grown in controlled conditions. This strategy lessens the demand for artificial fertilizers while also promoting healthier crops and lessening the impact on the environment. Micropropagated banana plants demonstrated consistent growth, early fruiting, and increased yield in contrast to conventionally propagated plants. Tissue-cultured plants exhibit strong development and genetic uniformity, which contribute to these advantages and more consistent and dependable harvests. By promoting efficient land use and reducing the need for chemical inputs, tissue culture enhances sustainability in the production of bananas. The growing demand for bananas may be met without requiring the increase of agricultural land thanks to tissue culture techniques, which enable the large-scale production of enhanced banana cultivars at a quick rate. Furthermore, using planting material free of disease lowers input costs and crop losses, supporting more environmentally friendly farming methods.

Modeling the transmission dynamics of banana bunch top disease in banana plants

Banana Bunch Top Disease (BBTD) is a severe viral infection that poses a significant threat to banana production in tropical regions. Caused by the Banana Bunchy Top Virus (BBTV) and transmitted primarily by the banana aphid (Pentalonia nigronervosa), BBTD results in stunted growth and poor fruit production, leading to substantial economic losses. This paper presents a mathematical model to analyze the transmission dynamics of BBTD in banana plants and evaluate various control strategies using the Next Generation Matrix approach to compute the basic reproduction number R0. The study reveals that effective control of aphid populations, timely removal of infected plants, and the use of virus-free planting material significantly reduce the spread of BBTV and improve plant health. Increasing the removal rate γ and managing the transmission rate β are crucial strategies for controlling the disease, while elevated aphid infection rates λ and progression rates σ exacerbate its spread. Numerical simulations highlight the effectiveness of targeted interventions, including the application of pesticides and the introduction of resistant banana varieties. The study recommends that, in addition to current control measures, more focus should be placed on comprehensive monitoring programs, early detection of symptoms, and community education on BBTD management strategies. Enhanced efforts in these areas are crucial to reducing the incidence of BBTD and improving the resilience of banana crops.

Geospatial analysis of Musa acuminata cultivation using multi-temporal remote sensing – Uthal Balochistan

Climate change has made researchers realize there is a need to focus on crops consumed staple, including fruits.Uthal is the districts headquarter of Lasbela and has long been known for its valuable banana production. Due to scarcity of rainfall, aquifers are not recharging significantly, causing slow decrease in the Musa acuminate (common name banana) crop. Banana is a significant source of earnings for local cultivators. The primary objective of the present study is the fluctuation assessment of banana cultivation of Uthal. The methodology has been designed in three steps. The first was ground-truthing with the help of GPS survey of banana cultivation areas, the second, classification of greenery and banana crops based on Landsat 8 images via EVI and NDVI remote sensing tools. The third and last step was combined both data along with government published data for the accuracy assessment of banana cultivation areas. As per extracted results from NDVI, the share of banana total cultivated land of the study area has declined concerning the overall cultivated land of the study area which reduced from 1115 to 879 ha between 2013 and 2022. Results identified that the EVI-NDVI-GPS model is more significant for monitoring agricultural activities. The present study is an advanced contribution to the scientific field of Banana cultivation in Uthal.

Combining cyclic lipopeptides and cinnamon extract enhance antifungal activity against Fusarium oxysporum strains pathogenic to banana and delay Fusarium wilt under greenhouse conditions

AbstractFusarium wilt of banana (FWB) caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc) is a widely distributed disease that generates devastating losses in banana production. Foc belongs to the Fusarium oxysporum species complex (FOSC) which includes several evolutionary lineages. Nine of them are pathogenic to banana such as F. phialophorum, F. grosmichelli, F. duoseptatum and the most aggressive F. odoratissimum tropical race 4 (TR4). No control method has been successfully implemented to manage FWB, then enhancing the potential of management approaches can avoid or delay disease epidemics and reduce disease severity. Here we determined the antifungal effect of different plant-based extracts against Foc in vitro, and whether the combination of cinnamon (Cinnamomum zeylanicum) extract and Bacillus tequilensis EA-CB0015 cyclic lipopeptides had an additive effect against different Foc lineages in vitro and against FWB in banana plants in greenhouse. We found, from 17 plant-based natural extracts, that cinnamon was highly active against Foc strain IB (race 1). Furthermore, cinnamon and cyclic lipopeptides inhibited different strains of various evolutionary lineages of Foc belonging to race 1 and TR4, and their combination increased in 1.4-fold the effect of the single extracts in vitro. Our results showed that soil concentration of F. odoratissimum TR4-II5 decreased by 1000-fold when treated with the combination of 488 mg L−1 cinnamon and 128 mg L−1 lipopeptides in a soil microcosm system after 5 days of incubation, followed by a partial population recovery after 21 days. In greenhouse experiments, the combination reduced external but not internal FWB symptoms, and cinnamon extract had a significant impact on internal plant symptoms. Taken together, the effect of cyclic lipopeptides with cinnamon extract on Foc supports their function towards delaying the effect of disease progression and suggests that the combination enhances the effect of the single extracts.

A novel in vitro shoot excision technique for enhancing proliferation in banana cv. Chini Champa

Micropropagation technology has been commercially exploited for mass multiplication of banana. Several parameters such as nutrient media, explants, culture conditions etc have been standardized. However, a novel in vitro shoot excision technique designed to enhance the proliferation rate of banana cv. Chini Champa has been examined for the first time. We meticulously examined the impact of excision angles (45º and 90º) and excision site (Tip, middle and base) during shoot proliferation stage. Our findings unequivocally demonstrate that employing a 45º angle excision and tip excision yield the highest multiplication rates and biomass accumulation, surpassing other excision angles and methods. The substantial enhancement in shoot numbers, growth and biomass underscores the potential of this technique for improving banana propagation protocols, offering a valuable tool for sustainable banana production.

Potential Valorization of Banana Production Waste in Developing Countries: Bio-Engineering Aspects

Plant food production generates a lot of by-products (BPs). These BPs are majorly discarded into the environment, polluting it, or into landfills where they just decompose, providing no benefit and taking up storage space, causing financial costs. These plant BPs are biodegradable, but reusing them may provide a better outcome and profit. The vast majority of plant-based food BPs are polysaccharide polymers like gums, lignin, cellulose, and their derivatives. It is possible to utilize plant food production waste, like banana peels, leaves, pseudostems, and inflorescences, to produce bioethanol, single-cell protein, cellulase, citric acid, lactic acid, amylase, cosmetics, fodder additives, fertilizers, biodegradable fibers, sanitary pads, bio-films, pulp and paper, natural fiber-based composites, bio-sorbents, bio-plastic, and bio-electricity in the agro-industry, pharmaceutical, bio-medical, and bio-engineering fields. Moreover, the use of banana BPs seems to be a way of dealing with many issues in underdeveloped countries, providing a clean and ecological solution. The suggested idea might not only reduce the use of plastic but also mitigate waste pollution.