Bamboo Growth Research Articles

A clean and novel drying method for bamboo colorization and in-situ surface wax utilization

Round bamboo is considered an environmentally friendly product, with surface color crucial to its value. Surface wax is crucial for the growth and development of bamboo, but can alter the color of round bamboo and easily detach under the influence of heat and humidity, leading to uneven colorization. By controlling the drying temperature at 90 ℃, the surface wax can be uniformly levelled on the bamboo’s surface, enhancing its color to a consistent deep red. Chromaticity measurements show L* values between 29.58 and 34.68 and a* values from 5.58 to 6.04 at 90 ℃. DSC test shows that the wax completely melted and levelled evenly at 90 ℃, solidifying uniformly on the bamboo surface upon drying, thus realising in-situ utilization of the wax. FTIR, GC-MS and XPS analyses reveal that drying at 90 ℃ leads to an increase in chromogenic substances within the wax and bamboo green, along with heightened intensity of unsaturated functional groups, and deepening of surface color, ultimately achieving the colorization of the round bamboo. Furthermore, the prepared round bamboo exhibits excellent pencil hardness (5 H), adhesion, and glossiness (5.38 GU), while maintaining its mechanical properties. This innovative method integrates drying, colorization, and wax utilization in one step, offering a novel strategy for enhancing round bamboo’s quality.

Subgenome asymmetry of gibberellins-related genes plays important roles in regulating rapid growth of bamboos

Rapid growth is an innovative trait of woody bamboos that has been widely studied. However, the genetic basis and evolution of this trait are poorly understood. Taking advantage of genomic resources of 11 representative bamboos at different ploidal levels, we integrated morphological, physiological, and transcriptomic datasets to investigate rapid growth. In particular, these bamboos include two large-sized and a small-sized woody species, compared with a diploid herbaceous species. Our results showed that gibberellin A1 was important for the rapid shoot growth of the world's largest bamboo, Dendrocalamus sinicus, and indicated that two gibberellins (GAs)-related genes, KAO and SLRL1, were key to the rapid shoot growth and culm size in woody bamboos. The expression of GAs-related genes exhibited significant subgenome asymmetry with subgenomes A and C demonstrating expression dominance in the large-sized woody bamboos while the generally submissive subgenomes B and D dominating in the small-sized species. The subgenome asymmetry was found to be correlated with the subgenome-specific gene structure, particularly UTRs and core promoters. Our study provides novel insights into the molecular mechanism and evolution of rapid shoot growth following allopolyploidization in woody bamboos, particularly via subgenome asymmetry. These findings are helpful for understanding of how polyploidization in general and subgenome asymmetry in particular contributed to the origin of innovative traits in plants.

Expression and drought functional analysis of one circRNA PecircCDPK from moso bamboo (Phyllostachys edulis).

Drought stress can affect the growth of bamboo. Circle RNAs (CircRNAs) have been found to play a role in drought stress in plants, but their function in moso bamboo is not well understood. In previous studies, we observed that under drought stress, the expression of some circRNAs were altered and predicted to be involved in calcium-dependent protein kinase phosphorylation, as indicated by KEGG enrichment analysis. In this study, we cloned a circRNA called PecircCDPK in moso bamboo that is responsive to drought stress. To further investigate its function, we constructed an overexpression vector using flanking intron sequences supplemented by reverse complementary sequences. When this vector was transferred to Arabidopsis plants, we observed that the roots of the transgenic lines were more developed, the water loss rate decreased, the stomata became smaller, and the activity of antioxidant enzymes increased under drought stress. These findings suggest that overexpression of PecircCDPK can enhance the drought resistance of Arabidopsis thaliana, providing valuable insights for the breeding of moso bamboo with improved resistance to drought.

Characteristic Analysis of the Soil Bacterial Community Structure of Dendrocalamus brandisii from Seven Geographical Provenances in Yunnan Province

Soil is the basis of bamboo growth and quality formation of bamboo shoots and has an important contribution to the sustainable development of agriculture. To this end, We studied the soil properties and microbial communities of Dendrocalamus brandisii by collecting twenty-one soil samples from its seven typical geographic provenances in Yunnan Province, China. Bacterial 16S rRNA gene amplicons were used to detect soil bacteria and predict bacterial functions using Tax4Fun. The results indicated that the soil bacterial diversity indices (ACE, Chao1, Simpson, and Shannon) were significantly different among different geographical provenances. The dominant bacterial groups at the phylum level in all seven regions were Proteobacteria (19.78~29.06%), Actinobacteria (13.53~30.01%), Chloroflexi (8.03~31.47%), and Acidobacteria (7.12~19.17%), with markedly different constitution proportions. Total phosphorus, available potassium, and pH were the main environmental factors affecting soil bacterial communities. There were significant differences in the secondary metabolic pathways and phenotypes of soil bacterial functions, exhibiting a diversity of functions. The geographical variables of the soil bacterial community in D. brandisii varied with spatial scales. Environmental factors such as available potassium (AK), pH, and total nitrogen (TN) have an impact on soil bacterial communities.

A PLATZ transcription factor PhePLATZ8 from Moso bamboo (Phyllostachys edulis) plays a positive role in regulating growth and abiotic stress tolerance

Moso bamboo is recognized as a rapid-growing plant with abundant lignocellulosic content, making it a crucial non-timber forest resource on a global scale. The growth of Moso bamboo is influenced by the rapid division and elongation of its internode meristem cells, but the molecular mechanism behind this process is still unclear. Here, by combining RNA-seq data from Moso bamboo internode samples with gene co-expression network and gene functional annotation enrichment analysis, a key candidate gene named PhePLATZ8, which regulates cell division in the internodes of Moso bamboo, has been screened out. PhePLATZ8 is localized to the nucleus and cytoplasm. The phenotype of transgenic Arabidopsis thaliana plants showed that PhePLATZ8 positively regulated the organ development of transgenic plants, including hypocotyl length, plant height, petiole length, blade shape, and seed size. Overexpression of PhePLATZ8 also significantly improved cell proliferation activities in the hypocotyl calluses of transgenic plants. qRT-PCR and luciferase assays showed that PhePLATZ8 was involved in the regulation of cell proliferation by activating the expression of several GRFs in A. thaliana and Moso bamboo. Futhermore, PhePLATZ8 was significantly induced by abiotic stress, and overexpression of PhePLATZ8 increased the tolerance of transgenic A. thaliana plants to drought and salt stress. Overall, our current study initially explored the role of PhePLATZ8 in the process of rapid growth and stress response of Moso bamboo, providing genetic resources for future molecular breeding of Moso bamboo.

Stoichiometric Homeostasis of N and P in the Leaves of Different-Aged Phyllostachys edulis after Bamboo Forest Expansion in Subtropical China

Stoichiometric homeostasis is an important mechanism in maintaining ecosystem structure, function, and stability. Phyllostachys edulis (moso bamboo) is a typical clone plant, forming pure bamboo forests or bamboo–wood mixed forests by expanding rhizomes around. Studying the stoichiometric homeostasis characteristics of moso bamboo at different ages after expansion contributes to a deeper understanding of the stability of bamboo forest ecosystems, and is of great significance for expanding the research scope of ecological stoichiometry. Based on the stoichiometric internal stability theory, the nitrogen (N) and phosphorus (P) elements in the soil and plants of typical moso bamboo forests in Tianbaoyan National Nature Reserve of Fujian Province were determined, and the internal stability index (H) of bamboo leaves of different ages (I-du, II-du, III-du, and IV-du bamboos) was calculated. The results showed that the dependence of moso bamboo on soil nutrients and the ability of moso bamboo to regulate nutrient elements were both significantly affected by the plant’s age. Under the condition of the same soil nutrients (N, P), the content of N and P in bamboo leaves decreased significantly with the increase in bamboo age. The limiting effect of phosphorus on the growth and development of moso bamboo was greater than that of nitrogen, and the limiting effect of phosphorus on aged bamboo was greater than that of young bamboo. The stoichiometric internal stability index of N and P in bamboo leaves is HN:P > HN > HP, which means that the internal stability of moso bamboo is closely related to the limiting elements. Therefore, the regulation ability of the internal stability of moso bamboo of different ages makes it grow well in the changeable environment, has stronger adaptability and competitiveness, and the leaf internal stability of I-du bamboo was higher than that of other ages, which may be one of the reasons for its successful expansion to form a stable bamboo stand structure.

Effects of climate warming on soil nitrogen cycles and bamboo growth in core giant panda habitat

Climate change can pose a significant threat to terrestrial ecosystems by disrupting the circulation of soil nitrogen. However, experimental analyses on the effect of climate change on soil nitrogen cycles and the implications for the conservation of key wildlife species (i.e., the giant panda, Ailuropoda melanoleuca) remain understudied. We investigated the effects of a 1.5 °C, 3 °C, and 4.5 °C temperature increase on nitrogen distribution in different soil layers of bamboo forest via an in-situ experiment and assessed the implications for the growth and survival of arrow bamboo (Bashania faberi), a critical food resource for giant pandas. Our results showed that warming treatments generally increased soil N content, while effects differed between surface soil and subsurface soil and at different warming treatments. Particularly an increase of 1.5 °C raised the subsurface soil NO3-N content, as well as the content of N in bamboo leaves. We found a significant positive correlation between the subsurface soil NO3-N content and the N content of arrow bamboo. An increase of 3–4.5 °C raised the content of total N and NO3-N in the surface soil and led to a reduction in the total aboveground biomass and survival rate of arrow bamboo. Limited warming (e.g., the increase of 0–1.5 °C) may promote the soil N cycle, raise the N-acetylglucosaminidase (NAG) enzyme activity, increase NO3-N in subsurface soil, increase the N content of bamboo, and boost the biomass of bamboo – all of which could be beneficial to giant panda survival. However, higher warming (e.g., an increase of 3–4.5 °C) resulted in mass death of bamboo and a large reduction in aboveground biomass. Our findings provide a cautiously optimistic scenario for bamboo forest ecosystems under low levels of warming over a short period of time, but risks from higher levels of warming may be serious, especially considering the unpredictability of global climatic change.

A bamboo 'PeSAPK4-PeMYB99-PeTIP4-3' regulatory model involved in water transport.

Water plays crucial roles in expeditious growth and osmotic stress of bamboo. Nevertheless, the molecular mechanism of water transport remains unclear. In this study, an aquaporin gene, PeTIP4-3, was identified through a joint analysis of root pressure and transcriptomic data in moso bamboo (Phyllostachys edulis). PeTIP4-3 was highly expressed in shoots, especially in the vascular bundle sheath cells. Overexpression of PeTIP4-3 could increase drought and salt tolerance in transgenic yeast and rice. A co-expression pattern of PeSAPK4, PeMYB99 and PeTIP4-3 was revealed by WGCNA. PeMYB99 exhibited an ability to independently bind to and activate PeTIP4-3, which augmented tolerance to drought and salt stress. PeSAPK4 could interact with and phosphorylate PeMYB99 in vivo and in vitro, wherein they synergistically accelerated PeTIP4-3 transcription. Overexpression of PeMYB99 and PeSAPK4 also conferred drought and salt tolerance in transgenic rice. Further ABA treatment analysis indicated that PeSAPK4 enhanced water transport in response to stress via ABA signaling. Collectively, an ABA-mediated cascade of PeSAPK4-PeMYB99-PeTIP4-3 is proposed, which governs water transport in moso bamboo.

Genome-wide identification, characterization, and expression pattern analysis of the JAZ gene family in Moso bamboo during rapid shoot development

Moso bamboo (Phyllostachys heterocycla (Carriere) Matsum.), a member of the bamboo subfamily within the Gramineae family, is a globally significant economic forest resource, notable for its swift growth. JAZ proteins, crucial in the jasmonic acid signaling pathway, play essential roles in plant growth, development, and responses to various biotic and abiotic stresses. This study identifies 22 JAZ genes in moso bamboo using bioinformatics. We conducted a comprehensive analysis of their physicochemical properties, gene structure, chromosomal localization, conserved structural domains and motifs, cis-acting regulatory elements, and evolutionary relationships. Furthermore, by mining transcriptome data, we delineated the expression patterns of the JAZ gene family during the rapid shoot development phase in moso bamboo. This analysis suggests a significant role for the JAZ genes in the growth and development of moso bamboo. Additionally, we predicted transcription factors that regulate JAZ and analyzed protein interactions to construct an initial JAZ-related regulatory network. This study provides a crucial foundation for understanding the functions of the JAZ gene family and elucidating the molecular mechanisms underpinning the rapid shoot development in moso bamboo.

Rowth of Betung Bamboo (Dendrocalamus asper) and Food Crop Production Under Agroforestry Bamboo Systems

One of the main species in community forests is betung bamboo (Dendrocalamus asper). Optimizing land use under bamboo can be achieved by cultivating food crops. This research aimed to determine the growth of betung bamboo and the production of food crops. The experimental design used a split plot design with four cultivation patterns as the main plots: agroforestry bamboo pattern i.e. agroforestry bamboo + rice (P1), agroforestry bamboo + rice + cassava (P2), agroforestry bamboo + rice + taro (P3), and mixed food crops (rice + cassava + taro) (P4), while the subplots were rice varieties: rindang 2 (V1), protani (V2), and unsoed (V3). The observed parameters were bamboo growth, soil fertility, and food crop production. The results showed that seven-year-old betung bamboo had an average of 6.01 mature stems per clump, an average plant height of 7.23 m, and a stem diameter of 7.12 cm. The numbers of young stems and shoots per clump was 1.45 and 3.71. The highest betung bamboo growth was in the agroforestry pattern (length: 8,49 m, diameter: 7.17 cm, thickness: 1.95, total weight: 21.31 kg). The highest rice yield was observed in the P2V1 treatment (4.17 kg). The highest cassava tuber yield per plant was observed in P4 (2.12 kg). The taro tuber yield was relatively higher in the agroforestry pattern with a distance of >1 m from the bamboo clump (0.52 kg plant-1). The land equivalent ratio (LER) and area time equivalent ratio (ATER) of bamboo and food crop agroforestry have a value above 1, so it is more efficient in land.

Spatiotemporal dynamic changes in transpiration in the shoot sheath and its relation to water transportation during rapid growth of Moso bamboo

IntroductionTranspiration is the driving force of water transport, which plays a crucial role in the rapid growth of bamboo shoots. Nonetheless, the transpiration changes that occur in the shoot sheath of Moso bamboo during the leafless stage and the physiological processes involved in water transport from the mother bamboo to bamboo shoots are not completely understood.MethodsThis study investigated the temporal dynamics of the transpiration rate (Tr) and stomatal conductance (gs) of the sheaths from bamboo shoots to culms and diurnal variations in Tr and gs as well as the spatial dynamics of Tr and gs in various parts of the shoot sheaths. Water distribution patterns in bamboo shoots were analyzed using the isotope tracer method, and the water transportation path in bamboo shoots was determined by soaking the shoots in fuchsine dye solution.ResultsWe observed that the Tr was higher in bamboo shoots at heights of 4 and 7 m compared to that at 0.5, 1, 2, and 13 m, with rates ranging from 6.8 mmol/m2/s1 to 8.3 mmol/m2/s. Additionally, the Tr and gs of the shoot sheath were lower at noon, but higher in the morning and evening. The Tr in the lower parts of the culm sheath was higher than that that in the upper part (height: 0.5–4 m). However, when injected into the mother bamboo, D2O was not immediately transported to the shoots via the mother culms but was transported upward through the vascular bundle, with a larger vascular bundle near the pulp cavity transporting high volumes of water.DiscussionThese findings provide the foundation for further studies on the rapid growth of Moso bamboo and establish a theoretical basis for water management during its shoot developmental period.

The scaling relationships between leaf sheath size and leaf lamina size in six bamboo species

ABSTRACT The scaling relationship between functional traits of leaf sheath and leaf lamina in bamboo is of significant importance in regulating bamboo growth, yet related studies are lacking. In this study, we collected a total of 2006 leaves from six bamboo species (including Pleioblastus argenteostriatus, Pleioblastus chino var. hisauchii, Pleioblastus fortunei, Pleioblastus kongosanensis f. aureostriatus, Pleioblastus maculatus, and Pleioblastus viridistriatus) growing in Nanjing, China, and measured leaf sheath length (SL), leaf sheath fresh mass (SFM), leaf lamina length (LL), leaf lamina area (LA), and leaf lamina fresh mass (LFM). We used reduced major axis regression protocols to fit the scaling relationships between various measures of leaf lamina and leaf sheath. For each individual species and the pooled data of six species, allometric relationships were found in lamina (LFM vs. LA) and sheath (SFM vs. SL), respectively. We also found robust allometric relationships between leaf sheath and leaf lamina (LA vs. SL and LFM vs. SL). These results revealed the strong coupling among leaf traits in terms of morphology and mass, providing insights into the understanding of the physiological process of bamboo growth.

A nondestructive method using X-ray micro computed tomography for variation analysis of bamboo nodes’ vascular bundle among species

Bamboo node is a specialized structure that has great effect on both bamboo growth, physical-mechanical properties, and its utilization. Compact structure and particularly high hardness make bamboo node very difficult for slice specimens preparation. In this study, in order to explore the differences in anatomical characteristics of bamboo nodes' vascular bundles among species, 3D structures of bamboo nodes in four bamboo species (Bambusa emeiensis, Phyllostachys edulis, Pleioblastus amarus, and Bambusa rigida) were reconstructed by the micro-CT technique. The characteristics of vascular bundles were comparatively analyzed based on the CT images. The results showed that the vascular bundle morphology of Pleioblastus amarus and Bambusa rigida changed significantly from node to internode. The areas of fiber cells in the middle part along the radial direction of bamboo culm wall has the largest area (0.204 mm2), followed by the inner part (0.151 mm2) and the outer part (0.072 mm2). The area of transport tissue in the inner part along the radial direction of bamboo culm wall has the largest area (0.046 mm2), followed by the middle part (0.028 mm2) and the outer part (0.002 mm2). The bending vascular bundles in bamboo node can be divided into thick and thin types. The average thickness of the thin bending vascular bundles among the four bamboo species was 125 μm, with the thinnest value of 109 μm. While, the average thickness of thick bending vascular bundles was 265 μm, with the thickest value of 335 μm. The number proportion of thick vascular bundles was 60.8%, 42.5%, 32.9%, and 10.7% for Bambusa rigida, Bambusa emeiensis, Pleioblastus amarus, and Bambusa rigida. This study set up a nondestructive and efficient method for 3D anatomical structure characterization of bamboo node and the variation analysis in bamboo nodes’ vascular bundles.

Nitrogen fertilization practices alter microbial communities driven by clonal integration in Moso bamboo

Nitrogen (N) fertilization is crucial for maintaining plant productivity. Clonal plants can share resources between connected ramets through clonal integration influencing microbial communities and regulating soil biogeochemical cycling, especially in the rhizosphere. However, the effect of various N fertilization practices on microbial communities in the rhizosphere of clonal ramets remain unknown. In this study, clonal fragments of Moso bamboo (Phyllostachys edulis), consisting of a parent ramet, an offspring ramet, and an interconnecting rhizome, were established in the field. NH4NO3 solution was applied to the parent, offspring ramets or rhizomes to investigate the effect of fertilization practices on the structure and function of rhizosphere microbial communities. The differences in N availability, microbial biomass and community composition, and abundance of nitrifying genes among rhizosphere soils of ramets gradually decreased during the rapid growth of Moso bamboo, irrespective of fertilization practice. The soil N availability variation, particularly in NO3−, caused by fertilization practices altered the rhizosphere microbial community. Soil N availability and stable microbial biomass N in parent fertilization were the highest, being 9.0 % and 18.7 %, as well as 60.8 % and 90.4 % higher than rhizome and offspring fertilizations, respectively. The microbial network nodes and links in rhizome fertilization were 1.8 and 7.5 times higher than in parent and offspring fertilization, respectively. However, the diversity of bacterial community and abundance of nitrifying and denitrifying genes were the highest in offspring fertilization among three practices, which may be associated with increased N loss. Collectively, the rhizosphere microbial community characteristics depended on fertilization practices by altering the clonal integration of N in Moso bamboo. Parent and rhizome fertilization were favorable for N retention in plant-soil system and resulted in more stable microbial functions than offspring fertilization. Our findings provide new insights into precision fertilization for the sustainable Moso bamboo forest management.

Morphological Characteristics and Growth Performance of Bambusa vulgaris Schrad. Ex J. C. Wendl in Selected Agro-Ecologies of Uganda

Bamboo is a versatile and valuable resource that has been shown to have social, ecological, and economic benefits in many countries worldwide. Even though Uganda has a suitable environment for bamboo growth, it remains underutilized. One of the key challenges in promoting bamboo farming is the need for more information about the growth performance of different bamboo species in various site conditions. Therefore, this study determined the morphological characteristics and growth performance of Bambusa vulgaris in Uganda. A total of 24 bamboo farms were selected from a list of bamboo farmers provided by key informants using stratified sampling. These farms had relatively uniform B. vulgaris stands and were drawn from Uganda's three agroecological zones (AEZs): the Southwestern, West Nile, and Lake Victoria crescent agro-ecological zones. Eight temporary sample plots were established in each zone, and various growth parameters were measured. One-way Analysis of Variance (ANOVA) followed by Sidak post-hoc test was used to compare the performance of Bambusa vulgaris across the different agro-ecological zones based on growth parameters. The results of the study showed that the total above-ground biomass was highest in the Lake Victoria crescent (24.773 kg/ha), followed by the West Nile (17.141 kg/ha) and the Southwestern (15.125 kg/ha) agro-ecological zones. The growth rate of B. vulgaris after three years was found to be similar to the outstanding growth rate mentioned in the literature. The growth performance of B. vulgaris was notably better in the L. Victoria crescent compared to the West Nile and Southwestern highlands – suggesting that efforts to promote the growing of B. vulgaris should prioritize the L. Victoria crescent agro-ecological zone. Overall, this study provides valuable information on the growth performance of B. vulgaris in different agro-ecological zones in Uganda. This information can be used to guide the promotion and development of bamboo farming in the country, which has the potential to improve livelihoods and contribute to sustainable development

Deciphering the regulatory role of PheSnRK genes in Moso bamboo: insights into hormonal, energy, and stress responses

The SnRK (sucrose non-fermentation-related protein kinase) plays an important role in regulating various signals in plants. However, as an important bamboo shoot and wood species, the response mechanism of PheSnRK in Phyllostachys edulis to hormones, low energy and stress remains unclear. In this paper, we focused on the structure, expression, and response of SnRK to hormones and sugars. In this study, we identified 75 PheSnRK genes from the Moso bamboo genome, which can be divided into three groups according to the evolutionary relationship. Cis-element analysis has shown that the PheSnRK gene can respond to various hormones, light, and stress. The PheSnRK2.9 proteins were localized in the nucleus and cytoplasm. Transgenic experiments showed that overexpression of PheSnRK2.9 inhibited root development, the plants were salt-tolerant and exhibited slowed starch consumption in Arabidopsis in the dark. The results of yeast one-hybrid and dual luciferase assay showed that PheIAAs and PheNACs can regulate PheSnRK2.9 gene expression by binding to the promoter of PheSnRK2.9. This study provided a comprehensive understanding of PheSnRK genes of Moso bamboo, which provides valuable information for further research on energy regulation mechanism and stress response during the growth and development of Moso bamboo.

Dynamics of rhizosphere microbial structure and function associated with the biennial bearing of moso bamboo

Moso bamboo (Phyllostachys edulis) is a valuable nontimber forestry product with a biennial cycle, producing abundant bamboo shoots within one year (on-year) and few shoots within the following year (off-year). Moso bamboo plants undergo clonal reproduction, resulting in similar genetic backgrounds. However, the number of moso bamboo shoots produced each year varies. Despite this variation, the impact of soil nutrients and the root microbiome on the biennial bearing of moso bamboo is poorly understood. We collected 139 soil samples and determined 14 major physicochemical properties of the rhizosphere, rhizoplane, and bulk soil in different seasons (i.e., the growing and deciduous seasons) and different years (i.e., on- and off-years). Based on 16S rRNA and metagenomic sequencing, major variations were found in the rhizospheric microbial composition during different seasons and years in the moso bamboo forest. Environmental driver analysis revealed that essential nutrients (i.e., SOC, TOC, TN, P, and NH4+) were the main drivers of the soil microbial community composition and were correlated with the on- and off-year cycles. Moreover, 19 MAGs were identified as important biomarkers that could distinguish on- and off-years. We found that both season and year influenced both the microbial community structure and functional pathways through the biosynthesis of nutrients that potentially interact with the moso bamboo growth rhythm, especially the on-year root-associated microbiome, which had a greater abundance of specific nutrients such as gibberellins and vitamin B6. This work provides a dynamic perspective of the differential responses of various on- and off-year microbial communities and enhances our understanding of bamboo soil microbiome biodiversity and stability.

Advancing circular economy for the growth, root development and elemental characteristics of bamboo (Bambusa vulgaris) on galamsey-degraded soil

Landscape restoration of degraded mining sites, crucial for agroecology and carbon farming in Ghana, remains poorly understood and underutilized. Bamboo has historically played a role in restoring such sites, but there is a need to integrate circular economy principles fully into harnessing its ecosystem services potential. In this study, we evaluated the impact of biochar and poultry manure amendments on galamsey-degraded mining sites to operationalize circular economy principles in ecosystem restoration, using Bambusa vulgaris as the test crop.Conducted in the Asikasu mining area of the Amansie Central District of Ashanti, a randomized complete block design (RCBD) experiment was used comprising five treatments: 1) 10 tonnes/ha biochar, 2) 10 tonnes/ha poultry manure, 3) Combined biochar and poultry manure at 10 tonnes/ha each, 4) Galamsey-degraded soil control, and 5) Forest topsoil control. Each treatment and control type was replicated three times. The experiment spanned from February 2020 to March 2021.The combined application of biochar and poultry manure resulted in the highest root density and average root diameter, demonstrating the potential for restoring galamsey-degraded sites to support bamboo-based agroecology. The elemental profiles of the bamboo varied in response to different treatments, indicating differing availabilities of micro- and macro-nutrients in the soil solution following soil amendments.This study underscores the viability of circular economy principles in ecosystem restoration, offering a pathway to sustainable land use and agroecological practices in Ghana's mining-impacted landscapes.

Community Structure and Diversity of Rhizosphere Soil and Endophytic Bacteria during the Period of Growth of Moso Bamboo Shoots.

The purpose of this study was to elucidate the community structure of rhizosphere soil bacteria and endophytic bacteria during the growth of moso bamboo (Phyllostachys edulis) shoots. This study collected the rhizospheric soil samples, tissue samples of rhizome roots, shoot buds, winter bamboo shoots, spring bamboo shoots, and samples of forest soil. Their metagenomic DNA was extracted, and the bacterial community structure and diversity characteristics were compared and analyzed using high-throughput sequencing technology. These samples enabled the identification of 32 phyla, 52 classes, 121 orders, 251 families, and 593 genera of bacteria. The phyla primarily included Proteobacteria, Acidobacteria, and Cyanobacteria among others. Proteobacteria was the dominant phylum in the samples of bamboo shoots and rhizome roots, whereas Acidobacteria was dominant in the rhizosphere and forest soil samples. The predominant genera of the rhizome root samples were Acidothermus, Bradyrhizobium and Acidobacterium, and the predominant genera of the soil samples were Acidothermus and Acidobacterium. This study preliminarily revealed the regularity between the growth and development of bamboo shoots and the changes in the community structure of rhizosphere soil and endophytic bacteria, which provides insights into the relationship between growth and the bacterial community structure in different stages of bamboo shoots.

REGENERASI ALAMIAH BAMBU DI AREAL GARAPAN KELOMPOK TANI HUTAN KARYA MAKMUR II DALAM TAMAN HUTAN RAYA WAN ABDUL RACHMAN

The existence of bamboo in forest areas is very important because the strong root structure of rhizomes allows bamboo to bind soil and water properly, and this is no exception in Wan Abdul Rachman Grand Forest Park. Bamboo growth is closely related to the influence of environmental conditions where bamboo grows. Fertile soil affects the growth of bamboo because the nutrients for bamboo growth are met. The objectives of this research are to determine the regeneration rate of bamboo and the ecological conditions of each species of bamboo. This research was conducted in the area at the cultivated area of Karya Makmur II Forest Farmers Group in Wan Abdul Rachman Grand Forest Park on January 2023. The method used was the exploration method which is carried out by exploring areas that are often overgrown with bamboo. The results of the research were observed that 5 species of bamboo each had a youth percentage of 17.4% for betung bamboo, 24.3% for andong bamboo, 23.9% for mayan bamboo, 12.7% for tali bamboo, and 15.8% for black bamboo. The bamboo grows at an altitude of 150--304 m above sea level, slope of 25--60%, soil acidity of 5.0--6.5, air temperature of 27.6--29.7oC, and humidity of 60--85% and has a soil texture in the form of loamy loam, clay and loam. The conclusion of this reaserch is, although bamboo can grow in a humid or dry environment, however bamboo's regeneration rate is higher in a humid environment.