The economic importance of the bamboo cultivation in Asia is well known, but the recent rise of interest in Europe required more deep studies on growing techniques. Among the bamboo species, the Phyllostachys pubescens (Carrière) J. Houz. is appreciated for its multiple uses: landscaping, timber and shoots production. In order to identify the best and sustainable combination of substrates and fertilization regimes, a nursery experimental trial was performed in 2016. Eight treatments (four substrates and two fertilization regimes) with 256 young plantlets divided into four randomized blocks were evaluated. The substrates used were: 40% peat, 40% coconut fibers, 20% pumice (standard substrate, S1); 30% peat, 40% coconut fibers, 10% rice husk, 20% pumice (S2); 30% peat, 40% coconut fibers, 20% rice husk, 10% pumice (S3); 30% peat, 40% coconut fibers, 20% wood fibers, 10% pumice (S4). The two fertilization regimes were: 1.6 g l-1 (A) and 0.8 g l-1 (B) NPK (16-11-10) Osmocote Exact®. All substrates were supplemented with a fungal inoculum (2.5 g l-1) and corrected with 2.5 × 10-3 g l-1 of CaCO3. The number of culms and leaves and the SPAD values of six plants of each blocks were monthly measured. Fresh and dry weights, before and after cultivation was recorded. Data were statistically analyzed. Results showed that rice husk should be used only in low percentage, but wood fiber can be more suitable for bamboo cultivation. The combination of alternative materials and low fertilization regimes (S1_B and S4_B) should be the key for a more sustainable cultivation for potted Phyllostachys pubescens in Europe.