Bambara Groundnut Research Articles

Effects of Cucumber Mosaic Virus on Yield Components and Yield of Bambara Groundnut

Bambara groundnut (Vigna subterranean L. Verdc) is a leguminous plant and its seeds are highly nutritious and can be considered as complete food. In addition to being a neglected crop; its production is limited by several constraints among which virus diseases are the most implicated. In order to contribute to the improvement of bambara groundnut in Nigeria, landraces of bambara groundnut were screened for resistance to Cucumber mosaic virus (CMV), one of the most damaging viruses in grain legumes. Treatments consisted of five landraces namely Black, Brown, Cream, Red nav, and Zebra coloured got from local farmers. These were arranged in Randomised Complete Block Design, with three replicates. Seedlings were infected with CMV isolate three weeks after sowing (WAS), during the 2019 and 2020 trials. The infected plants exhibited various symptoms of chlorosis, leaf deformation, growth retardation, and plant stunting. The sCMV caused significant reduction in plant height, the number of branches, leaves, pods, and seeds weight. In the 2019 and 2020 trials, 100 % incidence with the highest severity was observed in the Bambara groundnut landraces. Disease incidence varied between 41.6% in “Black” and 95.8 % in “Cream,” while disease severity was higher in “Cream” and “Red nav” with 5.0 respectively. “Black” and “Zebra” were the tallest with 16.8 cm among the infected as well as disease-free with 22.6 cm tall plants. “Black” landrace produced the highest number of pods in both CMV infections with 21 and disease-free with 33 pods respectively. Conversely, the number of days to flowering of 43 days was highest in infected plants of “Black”. The “Black” and “Zebra” landraces recorded uniform seed weights and the highest seed weight under CMV infection with 1.7 g per plant. The impact of the virus on yield loss was lowest in “Black” and “Zebra” landraces which indicated their possible use in the management of Cucumber mosaic virus disease in Bambara groundnut.

Process optimization of extruded noodles from Cocoyam (Colocasia Esculenta) and Bambara groundnut (Vigna Subterranea) flour

A noodle is a thin, long strip of spaghetti or a similar flour paste consumed with a sauce or taken with warm water with sugar and milk added as desired. In this regard, a modified noodle was produced from cocoyam with Bambara ground nut added to enhance the nutritional composition of the noodle. Central Composite Design (CCD) using Response Surface Methodology (RSM) was used in the optimization procedure. The aim was to develop the best mix of procedure variables that can give optimum formulation with a high protein. The process variables were feed composition (X1), barrel temperature (X2), and feed moisture composition (X3). The responses were moisture, protein, fat, and fiber. Regression models and residual plots were generated and adequacy was tested with regression coefficient (R2) and the lack of fit test. Results of the analysis of variance (ANOVA) indicated that the process variables had a significant effect on the protein (p < 0.05). Results for regression coefficients prove a fair fit of the model. The optimum noodle had 60g of Bambara ground nut, 120 oC barrel temperature, and 16% moisture content. Its responses were 10.66 mg/100g protein, 0.95 mg/100g fat, 1.60 mg/100g ash, and 1.75 mg/100g fiber. The optimized recipe shows improve nutrients.

English

Bambaranut (BN) (Vigna subterranea (L.)  Verdc.) Is a nutrient rich, drought tolerant food legume that can contribute to food security and community resilience. In Uganda however, production and utilization remain in relative obscurity. A study was conducted in Northern and Eastern Uganda to determine production and utilization practices and identify challenges and opportunities for BN improvement. Primary data on demographics, scale of production, utilization and challenges in BN production and utilization were collected from 371 farm households. Results show that more women (62%) than men (38%) are engaged in Bambaranut production for home consumption (100%), seed maintenance (50%) and sale of seeds/grains (44%). Production is low (averaging 0.2 tob 0.35 acre) with few farmers involved. The crop is predominantly utilised as food (100%), consumed as a fresh boiled snack (99%) and local dishes (79%). Major challenges that confront BN farmers include scarcity of seeds (73%), limited market (65%) and difficulty in harvesting (51%). Farmers overcome these challenges by saving own seeds, obtaining seeds from neighbours and producing on a relatively small scale. Improving production practices, fostering community-research linkages to support farmers in accessing seeds and harnessing the eager participation of the youth and men will stimulate increased production and better utilisation of BN in Uganda. Key words: Bambaranut, production, utilisation, practice, challenges.

Determination and Quantification of Phytochemicals from the Leaf Extract of Parthenium hysterophorus L. and Their Physio-Biochemical Responses to Several Crop and Weed Species

This current investigation was undertaken both in laboratory and glasshouse for documentation and quantification of phytochemicals from different parts of the parthenium (Parthenium hysterophorus L.) plant through LC-MS and HPLC to study their effect on two crops namely, Bambara groundnut (Vigna subterranean L.) and maize (Zea mays L.), and six different types of weed e.g., Digitaria sanguinalis, Eleusine indica, Ageratum conyzoides, Cyperus iria, Euphorbia hirta, and Cyperus difformis. The parthenium methanolic leaf extracts at 25, 50, 75, and 100 g L-1 were sprayed in the test crops and weeds to assess their physiological and biochemical reactions after 6, 24, 48, and 72 h of spraying these compounds (HAS). The LC-MS analysis confirmed seven types of phytochemicals (caffeic acid, ferulic acid, vanillic acid, parthenin, chlorogenic acid, quinic acid, and p-anisic acid) in the parthenium leaf extract that were responsible for the inhibition of tested crops and weeds. From the HPLC analysis, higher amounts in leaf methanol extracts (40,752.52 ppm) than those of the stem (2664.09 ppm) and flower extracts (30,454.33 ppm) were recorded. Parthenium leaf extract at 100 g L-1 had observed higher phytotoxicity on all weed species except C. difformis. However, all crops were found safe under this dose of extraction. Although both crops were also affected to some extent, they could recover from the stress after a few days. The photosynthetic rate, transpiration rate, stomatal conductance, carotenoid and chlorophyll content were decreased due to the application of parthenium leaf extract. However, when parthenium leaf extract was applied at 100 g L-1 for 72 h, the malondialdehyde (MDA) and proline content were increased in all weeds. Enzymatic antioxidant activity (e.g., superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) contents) were also elevated as a result of the sprayed parthenium leaf extract. The negative impact of physiological and biochemical responses as a consequence of the parthenium leaf extract led the weed species to be stressed and finally killed. The current findings show the feasibility of developing bioherbicide from the methanolic extract of parthenium leaf for controlling weeds, which will be cost-effective, sustainable, and environment friendly for crop production during the future changing climate.

Are South African Wild Foods the Answer to Rising Rates of Cardiovascular Disease?

The rising burden of cardiovascular disease in South Africa gives impetus to managerial changes, particularly to the available foods in the market. Since there are many economically disadvantaged groups in urban societies who are at the forefront of the CVD burden, initiatives to make healthier foods available should focus on affordability in conjunction with improved phytochemical diversity to incentivize change. The modern obesogenic diet is deficient in phytochemicals that are protective against the metabolic products of sugar metabolism, i.e., inflammation, reactive oxygen species and mitochondrial fatigue, whereas traditional southern African food species have high phytochemical diversity and are also higher in soluble dietary fibres that modulate the release of sugars from starches, nurture the microbiome and produce digestive artefacts that are prophylactic against cardiovascular disease. The examples of indigenous southern African food species with high horticultural potential that can be harvested sustainably to feed a large market of consumers include: Aloe marlothii, Acanthosicyos horridus, Adansonia digitata, Aloe ferox, Amaranthus hybridus, Annesorhiza nuda, Aponogeton distachyos, Bulbine frutescens, Carpobrotus edulis, Citrullus lanatus, Dioscorea bulbifera, Dovyalis caffra, Eleusine coracana, Lagenaria siceraria, Mentha longifolia, Momordica balsamina, Pelargonium crispum, Pelargonium sidoides, Pennisetum glaucum, Plectranthus esculentus, Schinziophyton rautanenii, Sclerocarya birrea, Solenostemon rotundifolius, Talinum caffrum, Tylosema esculentum, Vigna unguiculata and Vigna subterranea. The current review explains the importance of phytochemical diversity in the human diet, it gives a lucid explanation of phytochemical groups and links the phytochemical profiles of these indigenous southern African foods to their protective effects against cardiovascular disease.

Gelation profile of laccase-crosslinked Bambara groundnut (Vigna subterranea) protein isolate

Enzymatic crosslinking has gained attention in improving plant protein heat-induced gels, which are composed of weak network structures. The aim of the study was to investigate the effect of laccase crosslinking on the rheological and microstructural properties of heat-induced Bambara groundnut protein gels. The rheological properties of laccase-modified Bambara groundnut protein isolate (BPI11BPI- Bambara groundnut protein isolate.) gel formed in situ were investigated. Changes in viscoelastic properties were monitored during heating and cooling ramps and gel structure fingerprints were analyzed by frequency sweep. Laccase addition induced an initial protein structure breakdown (G″>G′) at an enzyme dose-dependent (1–3 U/g) before gel formation and stabilization. Gel point temperatures were significantly decreased from 85°C to 29°C (∼3 folds) with increasing laccase activity (0 to 3 U/g protein, respectively). For laccase crosslinked gels, G′ was substantially greater than G” (>1 log) with no dependency on angular frequency, which suggests the formation of relatively well-structured gels. The highest gel strength (tan δ of 0.09, G* of 555.51 kPa & An of 468.04 kPa) was recorded at a laccase activity of 2 U/g protein and the gels formed at this activity appeared homogeneous with compact lath sheet-like structure. The crosslinking effects of laccase were corroborated by the decrease in thiol and phenolic contents as well as the crosslinking of amino acids in model reactions. Overall, the use of laccase improved gel properties and significantly altered the gelation profile of BPI. Laccase-modified Bambara groundnut protein gels have potential to be used in food texture improvement and development of new food products. For instance, they can be used in plant-based milk products such as yoghurt and cheese.

Carbon source utilization pattern of soil bacterial microbiome of bambara groundnut rhizosphere at the different growth stages determines soil fertility

The diversity of microbes in the soil of plants is important for sustainable agriculture as these microbes are important in carrying out different functional processes to improve the soil and invariably plant growth. Inversely the presence of the crop also affects the types of microbial communities in the soil. In this study, bambara groundnut was grown during the planting season in South Africa, from November to March 2014/2015 and 2015/2016 and soil samples were taken after every 4 weeks after planting. Soil samples were taken 15 cm deep from 2 different landraces named VBR and VL. Microbial diversity was determined by soil microbial Carbon Source Utilization Profiles (CSUP) using BIOLOG™ GN2 plates. The abundance and richness of the soil microbes was also determined using the Shannon-Weaver and Evenness diversity indices. The diversity of the soil microbial population changed over the stages of plant growth, according to cluster analysis. Bacterial abundance and diversity were higher at 4 and 8 weeks after planting (WAP). The microbial abundance (richness index) in this study ranged from 0.64 to 0.94 with cultivar VL2 at 8 WAP being the highest while bulk soil (control), R2 was the lowest. The Shannon-Weaver index varied between 2.19 and 4.00 with the lowest corresponding to control while the highest was VL2 at 8 WAP. Carbon sources utilized by bacterial communities spread across the 96 carbon sources. The highest utilization of carboxylic acids, ester, amino acids and polymers and carbohydrates was found in the bacterial communities of the different landraces across growth stages. The highest utilization of alcohols, amides, amines, aromatic chemicals, brominated chemicals and phosphorylated chemicals was found in the control landraces. This indicates that the soil samples between 4 WAP and 12 WAP were richer in diversity of microbial species and their abundance. This soil diversity and richness is an indicator of the quality of the soil in order to increase crop yields and agricultural production.

Detection of Toxicogenic Molds in Some Legumes Sold in Local Markets of Ho, Ghana

Background: Legumes are plants that contain edible seeds and belong to the family Leguminosae with varying nutritional benefits to humans and animals. This study aimed to detect and identify toxicogenic molds on some legumes purchased from two local markets in Ho Municipality, Ghana.
 Methods: A total of 36 samples, including cowpea (n=9), soybean (n=9), brown bean (n=9), and Bambara bean (n=9) were randomly obtained from 2 local markets in the Volta region of Ghana. Culturing of the legume seeds were done on mycological media using serial dilution technique. Fungal species occurrence was also determined. Statistical Package for Social Sciences (SPSS) version 26 was used to analyze the data.
 Results: Fungal counts on cowpea, soybean, brown beans, and Bambara beans ranged between 1.91 and 2.84 log Colony Forming Units (CFU)/g on both media. There were no statistically significant differences (p>0.05) in the samples from the different vendors. The Moisture Content (MC) ranged between 6.74 and 12.15%, pH ranged between 6.27±0.03-6.53±0.02. A total of 13 fungal species belonging to 7 genera were isolated on SDA and OGYEA media; Aspergillus species (A. niger, A. terreus, A. flavus, A. fumigatus, A. ochraceus, A. parasiticus), Fusarium species (F. oxysporum), Trichoderma harzianum, Rhizopus species (R. stolonifer), Penicillium species (P. digitatum, P. verucosum), Rhodotorula mucilaginosa, and Mucor racemosus were recorded on the legumes.
 Conclusion: The presence of some mycotoxigenic fungi in legumes examined in this study showed the potential health hazards in the local people of Ho, Ghana.

Potentials of Cassava, Bambara Groundnut and Tigernut in Biscuit Production: A Review

Due to varying changes in lifestyle, economic level and urbanization in the developing countries, the consumption of biscuit and the development of food products using composite flour have increased and are attracting much attention from researchers in bakery and pastry productions. This work reviewed the potentials of cassava, bambara groundnut and tigernut composite flours in biscuit production. This study also includes studies on the use of flours from other tubers, legumes, cereals, and fruit sources in place of wheat flour to make a variety of food products in varying amounts. It was revealed that flour made from cassava, bambara groundnut and tigernut used to produce biscuit products has the capacity of maintaining similar characteristics to products made from full-wheat flour. The finished result demonstrates the cumulative impact of this option in terms of its functional and physicochemical features, as well as the health advantages of raw mixed flour and percentage blending.
 In addition, composite flour is an excellent new strategy for employing unusual food products becaue its use produced goods with a range of qualities and features, depending on the types and proportions of wheat flour used in the formulation.
 Also this review stated some possible challenges likely to be encountered in case Nigeria changes from use of wheat flour to composite flours and blends of non-wheat flours.

Intermediate Pyrolysis of Bambara Groundnut Shell (BGS) in Various Inert Gases (N2, CO2, and N2/CO2)

Energy from biomass is increasingly gaining attention amidst the environmental challenges of coal and fossil fuels. This study investigated the effects of inert gases (N2, CO2, and N2/CO2) on intermediate pyrolysis and product properties from Bambara Groundnut Shells (BGS) (shells from an underutilized crop, which has high nutritional values). N2/CO2 atmosphere roughly represents flue gas. The results showed that the inert gases did not significantly affect the yields of bio-oil, biochar, and syngas. The pH of bio-oil ranged from 5.2–5.8, indicating the minimum presence of acids in bio-oil. The CHNS analysis showed that all bio-oil and biochar had their carbon content within 50.04–60.49 wt.%. The FESEM resulted in a wide range of pore sizes in biochar produced in an N2/CO2 atmosphere. The GC-MS (Gas Chromatography-Mass Spectrometry) analysis revealed the presence of compounds which can be categorized as alkene, acid, benzene derivatives, ketone, phenol derivatives, alcohol, aldehyde, alkyl, and ester. However, the presence of N2/CO2 gas favored alcohol and phenol production significantly.

Performance of Bambara groundnut (Vigna subterranea [L.] Verdcourt) Genotypes Cropped on Plinthite Soil in the Semi Arid-Zone, Burkina Faso

Bambara groundnut (Vigna subterranea [L.] Verdcourt) is grown mainly as a food crop in Burkina Faso. Despite its high nutritional value, it has been among the most neglected crops in the country. This study was undertaken with the objective to investigating the effects of genotypes on the physiological traits, yield and yield related traits of the crop and thereby identify the genotypes having better performance for yield and yield related traits. The field experiment was conducted at the Tenkodogo University Centre site, during the 2021 rainy season. The experiment was laid out in randomized complete block design with four replications and eight genotypes were obtained from INERA germplam bank. Data were collected on number of days to 50% field emergence, number of days from sowing to 50% flowering, number of leaves per plant, plant height, plant spread, number of pods per plant, number of one seed per pod, number of two seeds per pod, weight of seeds per plant seed length, seed width,100-seed weight and yield. The data were subjected to the analysis of variance, and means were separated through Duncan’s Multiple Range Test at 95% confidence. The Pearson’s correlation coefficients between pair of characters were computed using SPSS 2.0. Results showed that significant and highly significant variations were observed among genotypes for almost all studied characters, except plant spread and number of one seed per pod. Majority of the characters had positive correlations. Most of the negative correlation was observed between physiologic and agronomic traits. The result showed that genotypes KVS97-2 (33.75 days; 1578.12 kg.ha-1); KVS360 (34.75 days; 1181 kg.ha-1) and KVS235 (34.5 days; 1167.19 kg.ha-1) performed better than others genotypes in yield parameters and had shorter flowering cycle.

Genetic diversity and population structure analyses of South African Bambara groundnut (Vigna subterranea [L]. Verdc.) collections using SNP markers

• Bambara groundnut productivity in South Africa is low (0.62 t/ha) compared to the attainable yield of the crop reaching up to 3 t/ha. • The low productivity is attributable to a lack of improved and high yielding cultivars that are locally bred and adapted in the country. • This paper presents baseline information on Bambara groundnut genetic profile of South African collections for effective breeding and conservation. • The magnitude of the genetic diversity and population structure of Bambara groundnut collections of South Africa using high throughput single nucleotide polymorphisms (SNP) markers are highlighted to guide current and future Bambara groundnut research and development by selection genetically unique genotypes for breeding. Bambara groundnut ( Vigna subterranea [L.] Verdc) is a highly nutritious grain legume with a significant potential to develop various commercial food and feed products. It is a resilient crop able to grow under harsh climates and poor soil conditions. Bambara groundnut productivity in South Africa is low (0.62 t/ha) compared to the attainable yield of the crop reaching up to 3 t/ha. The low productivity is attributable to a lack of improved and high yielding cultivars that are locally bred and adapted in the country. Genetically diverse and locally adapted Bambara groundnut landrace varieties and exotic collections are present in South Africa for strategic breeding to improve yield and yield-related traits and nutritional quality. The objective of this study was to determine the magnitude of the genetic diversity and population structure of Bambara groundnut collections of South Africa using high throughput single nucleotide polymorphisms (SNP) markers. Ninety-three genotypes were assessed using genotyping with 2286 SNP markers and some unique complementary morpho-agronomic traits of the crop. The mean genetic diversity value was 0.32, revealing moderate genetic differences among the assessed genotypes. Cluster and structure analyses grouped the test genotypes into two distinct categories. Further, the analysis of molecular variance partitioned the total genetic variation into among genotypes (90%), within genotypes (8%) and among populations (2%). The results revealed two heterotic groups for hybridization and selection programs. The following unique genotypes were selected: ARC Bamb 37 (with spreading growth type), ARC Bamb 49 (bunch type), ARC Bamb 61 (semi-bunch) and ARC Bamb 83 (spreading) using the SNP markers and desirable agronomic traits. The study provided new insight on Bambara groundnut genetic profiles of South African collections which will assist in conservation strategy and management of the crop for effective breeding.

Induced chlorophyll and morphological mutations in Bambara Groundnut (Vigna subterranea (L.) Verdc.)

The present study was conducted at K-block, University of Agricultural Sciences, Bengaluru. Theexperimental material for the present investigation consists of two varieties of Bambara groundnut havinggood agronomic base belonging to different accessions viz., SB-42 (light brown, round shape and whitehilum) and S165A (dark brownish, dark spotted surface and oval shape). Five different concentrations ofEMS 0.1, 0.2, 0.3, 0.4 and 0.5 per cent were used to treat the seeds. After the treatment, the seeds were sownin the field along with control (untreated seeds) to raise M1 generation. The M2 generation was raisedduring 2014 summer season by pooling all the seeds of M1 plants on plant to row basis along with controlplants. In both the varieties of Bambara groundnut, the mutagenic effectiveness and efficiency of mutagenictreatments varied not only between the two varieties but also between the treatments. A concentration of0.3 per cent EMS was more effective than the other concentrations of EMS. Hence 0.3 per cent EMS has beenregarded as LD50 for inducing useful mutations.

Variations of Nutrient and Antinutrient Components of Bambara Groundnut (Vigna subterranea (L.) Verdc.) Seeds

Bambara groundnut (BGN) fits the bill when it comes to an acceptable level of nutrient and mineral composition. BGN is a balanced food that can help eradicate food and nutritional insecurity if it is incorporated into the major food system. However, there is a large degree of variation in nutrient composition and antinutritional factors among BGN accessions. Here, we show the degree of variability of nutrient and antinutrient components such as percentage ash, moisture, protein, fat, tryptophan, tannin, and phytate contents in seeds of 95 accessions of BGN. Data were subjected to analysis of variance (ANOVA), followed by correlation and principal component analysis. Clustering was done to show the relatedness between the accessions in response to the various traits. A high level of heterogeneity was observed among the accessions for the various traits studied. PC1 and PC2 show 41.2% of the total observed variations. Cluster analysis grouped accessions into four main clusters. This study was able to confirm the high level of diversity in the components of nutrients and antinutrients previously reported in BGN. The results of this study are expected to aid in identifying parent lines for improved breeding programs.

Plant and Soil Core Mycobiomes in a Two-Year Sorghum-Legume Intercropping System of Underutilized Crops in South Africa.

Fungal communities form close beneficial (mutualists) or detrimental (pathogens) associations with their plant hosts. Their diversity and abundance can be affected by agricultural practices which include cropping systems such as rotations and intercropping. Despite the importance of cropping systems in increasing productivity, knowledge of the fungal mycobiome and the core inhabitants for under-utilised cereal and legume crops, particularly over a period, is still limited. The core mycobiomes in plant tissues and bulk soils of a cereal–legume intercrop were characterized over two years using high-throughput sequencing. The intercropping trial consisted of sorghum, Bambara groundnut, cowpea, dry bean, and soybean. A greater number of molecular operational taxonomic units (MOTUs) were found in plant tissues compared to those from the soils and between year one and year two. Principal coordinate analyses revealed that fungal communities for each year were relatively distinct, particularly for the soils. The core mycobiome was dominated by a Davidiellaceae sp. (Cladosporium), Didymellaceae sp. 1 (Phoma), Didymellaceae sp. 2 (Epicoccum), Fusarium sp. 2, Unidentified (Ascomycota), and Cryptococcus MOTUs that were present in all plant tissues and soils of year one and two. Other key MOTUs were only specific to a year, substrate, or crop. Although the mycobiome of sorghum were more distinct than the cores of the legumes, there were still MOTUs dominant across all of the crops. Characterization of this baseline core across two years provides insight into those fungi that are always present in these crops, and that could be utilized in improving crop performance and productivity.

Variation in protein content, starch components, selected minerals and their bioavailability in bambara groundnut accessions

IntroductionBambara groundnut is a highly nutrient dense underutilized orphan crop, and has the potential to address malnutrition and food security. The study objectives were to estimate the variation in the concentration of protein, starch, amylose, iron (Fe), zinc (Zn), phytic acid (PA) and mineral bioavailability, to identify superior bambara accessions and to determine the interrelationship between measured traits. Materialsand methods: Grain samples were collected from 59 accessions that were planted in two different locations over two seasons and used to measure the nutritional quality traits. Results: Significant variability among accessions in diverse locations and seasons for protein, Fe, Zn, and PA/Fe and PA/Zn ratios was observed. The average values of measured characteristics were protein (22.59 %), starch (36.52 %), amylose (21.15 %), Fe (38.83 mg kg−1), Zn (27.10 mg kg−1), PA (6239.24 mg kg−1), PA/Fe (17.74) and PA/Zn (22.87) content. Significant positive correlations between protein, Fe and Zn were observed and these traits were negatively correlated with starch, amylose, PA, PA/Fe and PA/Zn ratio. Accessions SB 4–4 A and M 3 were superior for more than one nutritional trait and had good mineral bioavailability. ConclusionSuperior accessions can be used as potential parents for the development of nutritionally improved bambara cultivars.

Structure, in vitro starch digestibility and physicochemical properties of starch isolated from germinated Bambara groundnut.

This study investigated the effect of germination time (0, 24, 48 and 72h) on the physicochemical characteristics, in vitro starch digestibility and microstructural changes in Bambara groundnut starch. The starch yield, lightness (L*) value, amylose content and resistant starch contents of isolated starches decreased significantly (p ≤ 0.05) with increasing germination time. Scanning electron microscopy revealed that starch from raw and germinated Bambara grains were smooth with no evidence of starch degradation and were mainly oval shaped, with some granule's irregular and kidney shaped. Water absorption capacity (1.33-1.90g/g), swelling power (2.12-16.53g/g), solubility index (1.14-13.04g/g), and dispersibility (75.92-86.47%) greatly increased as germination timed increased. Germination did not alter the X-ray diffraction pattern (Type-A) but increased the relative crystallinity of the starches. The peak gelatinization temperatures (73.23-73.91°C) of starch from germinated Bambara were significantly higher than native starch (72.81°C). Native starch and starch from germinated Bambara grains had substantially high proportion of resistant starch (approx. 73%) and high pasting temperatures (approx. 88°C). Conclusively, germination significantly changed starch structure at molecular level and impacted functionality.

The influence of Bambara groundnut (Vigna subterranean) flour on the nutritional, physical and antioxidant properties of steamed bread

ABSTRACT This study aimed to evaluate the influence of fortifying steamed breads with Bambara groundnut (BGN) flour (5%, 10%, 15% and 20%) on their physicochemical and antioxidant characteristics. The water absorption capacity of the flour increased with increasing BGN flour fortification levels, while oil absorption capacity decreased. The pH and leavening capacity of the BGN flour fortified dough decreased during incubation, whilst the titratable acidity for dough fortified with 15% and 20% BGN flour increased. The inclusion of BGN flour improved the protein, ash, and crude fibre contents of flour and steamed breads. The HunterLab colour values show that BGN flour increased the yellowness (b* value) of flour and steamed bread samples, while the lightness, redness and Chroma decreased. The antioxidant properties of flours and steamed breads increased significantly (p < 0.05) with the BGN flour fortification levels. Moreover, the weight and spread ratio of the breads increased, whilst the texture became soft. The BGN flour can be utilised as a functional ingredient to enhance the nutritional and antioxidant properties of cereal-based foods such as steamed bread.

CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects.

Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like “genome editing” are vital for modernizing crop breeding to provide game-changing solutions to some of the “must needed” traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.

Optimal production areas of underutilized indigenous crops and their role under climate change: Focus on Bambara groundnut

Food demand in Africa continues to outstrip local supply, and the continent currently spends over US$35 billion annually on food imports to supplement local deficits. With the advances in agronomy and breeding, commercial crops like maize (Zea mays) and soybean (Glycine max) in the region are under threat from climate change, decreasing rainfall and degraded lands. Unlike commercial crops that are generally adapted from other regions, underutilized indigenous crops are uniquely suited to local environments and are more resilient to climatic variations and tolerant to local pests and diseases. This study, done in Limpopo Province, South Africa, identifies optimal areas for cultivating Bambara groundnuts (Vigna subterannea), an indigenous crop suitable for arid and semi-arid regions. The aim is to promote the production of underutilized indigenous crops at a large scale with fewer resources, while still meeting local demand and reducing the food import budget. Suitability maps are delineated using a multicriteria decision method in a Geographic Information System (GIS). The procedure is important for diversifying farming systems, making them more resilient (to biotic and abiotic stresses and climate change) and more successful at enhancing water, food and nutritional security. With the province's limited water and land resources for agriculture expansion, promoting indigenous underutilized crops is a pathway to reduce water allocated to agriculture, thereby enhancing drought resilience and ensuring water, food and nutritional security. Large tracts of degraded agricultural land deemed unsuitable for adapted crops, and which may require costly land reclamation practices, can be used to cultivate underutilized crops that are adapted to extreme local conditions.