The aim of the work was to analyse biosynthetic gene clusters (BGC) of Bacillus velezensis ONU 553 based on bioinformatics approach. Methods. Identification of species was processed with tools of TYGS server; EzBioCloud was used to calculate ANI. Analysis of biosynthetic gene, bacteriocin, and antibiotic resistance gene clusters using antiSMASH, Bagel4, respectively. The results. It is shown that the results of identification, phylogenetic analysis and DNA-DNA hybridization (DDH) carried out in silico proved that the strain Bacillus velezensis ONU 553 belongs to the operational group B. amyloliquefaciens (OGBa). Sequences identified as possible phages and CpG-islands were found in the genome of our strain. 12 biosynthetic gene clusters (BGC) were identified using antiSMASH. One new cluster capable of synthesizing a new metabolite was identified (region 11). The presence of two clusters of bacteriocins in the genome of Bacillus velezensis ONU 553, which are assigned to uberolysin/carnocyclin and the antimicrobial peptide LCI based on the identification of the core gene, is shown. Conclusions. The preliminary identification of the Bacillus velezensis ONU 553 strain as a representative of the Bacillus velezensis strain of the B. amyloliquefaciens group (OGBa) was confirmed. The presence of gene clusters of secondary metabolites responsible for the synthesis of surfactins, polyene antibiotics, antimicrobial peptides, macrolide antibiotics and bacteriocins was shown. The obtained results indicate that the Bacillus velezensis ONU 553 strain is promising for use in the field of "Blue Biotechnology" for the development of new drugs with antimicrobial and antifungal activity.