Abstract
Escherichia coli strains isolated from case of colibacillosis in Russian poultry farms in the region of Perm Krai were analyzed for their sensitivity to main antibiotics and bacteriocins. Sensitivity profiles for 9 antibiotics and 20 bacteriocins were determined with the disc diffusion method and the overlay test, respectively. Further, with the PCR the presence of several bla and integron 1 genes was revealed and the phylogenetic group for each strain determined. Among the 28 studied E. coli strains 85.7% were resistant to at least three antibiotics, 53.6% to five or more drugs, and 10.7% to eight antibiotics. PCR revealed that the blaTEM gene was harbored by 71.4% of strains and the blaCTX-M gene by 53.6% of strains. The class 1 integrons were found in 28.6% of strains. All of the studied strains were insensitive to ten or more bacteriocins. More than 90% of the studied strains were insensitive to pore-forming colicins of group A and B colicins, while 60.7% were insensitive to colicins with DNase and RNase activity. All of the analyzed strains were insensitive to at least two of the tested microcins. Neither the antibiotic resistance profile nor the bacteriocin resistance profile correlated with phylogenetic group of the strains. Thus, the studied strains were shown to possess high levels of multiple resistance to antibiotics and insensitivity to bacteriocins.
Highlights
Escherichia coli are part of the mammalian and human intestinal microbiota
Escherichia coli strains isolated from case of colibacillosis in Russian poultry farms in the region of Perm Krai were analyzed for their sensitivity to main antibiotics and bacteriocins
This work has shown that the E. coli strains circulating in studied Russian poultry farms with cases of colibacillosis are resistant to a large number of antibiotics tested and that more than half of the studied strains are multidrug-resistant
Summary
Escherichia coli are part of the mammalian and human intestinal microbiota. They are widely distributed in the environment. Among the E. coli strains, not just commensal, and pathogenic, intestinal pathogenic E. coli strains (IPEC) and extraintestinal pathogenic E. coli (ExPEC), can be found. The E. coli has “two faces”; it is found in the intestinal microbiota of healthy poultry, and associated with extraintestinal diseases [2,3]. The avian pathogenic E. coli (APEC) is the causative agent of colibacillosis and septicemia in birds that leads to localized inflammation, most commonly presenting as perihepatitis, airsacculitis, and/or pericarditis and lymphocytic depletion of the bursa and thymus. APEC is the main cause of mortality in poultry farms [4].
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