Tomato Bacterial Wilt Research Articles

Biochar from different sources against tomato bacterial wilt disease caused by Ralstonia solanacearum

Biochar from different sources against tomato bacterial wilt disease caused by Ralstonia solanacearum

Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling

ObjectivesEvaluation of Streptomyces isolated from Udhagamandalam, The Nilgiris, Tamilnadu, India for the biocontrol potential against the phytopathogen Ralstonia solanacearum and its metabolite profiling. MethodsStreptomyces strains were screened for plant growth promotion and antagonistic activity against R. solanacearum in in-vitro. The metabolite profiling of strain UT4A49 was studied through gas chromatography coupled with mass spectroscopy (GC–MS) analysis. Further, the identified lead compound 2,4-Di-tert-butylphenol (2,4-DTBP) was analyzed for its mechanism of action through in-silico docking study. The bioorganic fertilizer (BOF) amended potential Streptomyces strain UT4A49 was studied for biocontrol activity against R. solanacearum on tomato seedlings through pot culture experiments. ResultsThe Streptomyces strain UT4A49 showed the promising production of indole acetic acid (IAA) (76.0 ± 2.0 µg/ml), siderophore, ammonia, amylase, protease, cellulase as well as it solubilized the phosphate in-vitro. The strain UT4A49 showed strong in vitro antagonistic activity against R. solanacearum with the inhibition zone of 19.83 ± 0.44 mm in diameter. The isolated major compound, 2,4-Di-tert-butylphenol was showed strong inhibition against R. solanacearum. In silico docking study revealed that the 2,4-DTBP showed lower binding energy score with all targeted proteins which found better protein–ligand binding stability. In the pot experiment, biocontrol activity of BOF amended Streptomyces strain UT4A49 showed significant biocontrol efficacy was 78.5% against R. solanacearum in tomato seedlings. The strain UT4A49 was identified as Streptomyces sp. based on its morphological and molecular properties. ConclusionsThe detailed literature survey revealed that this is the first report on strong bioactive properties of 2,4-DTBP against R. solanacearum. This present study proved that bioorganic fertilizer amended with Streptomyces sp. UT4A49 is a promising biocontrol agent for tomato bacterial disease control.

Synergistic Effect between Trichoderma virens and Bacillus velezensis on the Control of Tomato Bacterial Wilt Disease

(1) Background: Ralstonia solanacearum causes tomato bacterial wilt disease, one of the most serious tomato diseases. As the combination of Trichoderma virens (Tvien6) and Bacillus velezensis (X5) was more effective at controlling tomato bacterial wilt disease than a single agent, we investigated the synergistic effect of Tvien6 and X5 in controlling this disease; (2) Methods: The disease incidence, plant heights and weights, relative chlorophyll content (SPAD values), defensive enzymes (PPO, POD, and SOD) activities, and metabolome were estimated among four treatment groups (BR treatment, X5 + R. solanacearum (RS-15); TR treatment, Tvien6+ RS-15; TBR treatment, Tvien6 + X5 + RS-15; and R treatment, RS-15); (3) Results: The R treatment group had the highest disease incidence and lowest plant heights, plant weights, SPAD values, defensive enzyme activities, and D-fructose and D-glucose contents; the TBR treatment group had the lowest disease incidence and highest plant heights, plant weights, SPAD values, defensive enzyme activities, and D-fructose and D-glucose contents; (4) Conclusions: The results revealed that Tvien6 and X5 can both individually promote tomato plant growth, increase leaf chlorophyll content, enhance defensive enzyme activities, and induce the accumulation of D-fructose and D-glucose; however, they were more effective when combined.

NB-LRR genes: characteristics in three Solanum species and transcriptional response to Ralstonia solanacearum in tomato.

NB-LRR genes in the three Solanum species showed specific constitution characteristics and evolved multiple clusters and duplicates. Some genes could respond to biotic stresses such as tomato bacterial wilt. Nucleotide-binding and leucine-rich repeat (NB-LRR, NLR) is a largest resistance gene family in plants, which plays a key role in response to biotic stresses. In this study, NB-LRR genes in cultivated tomato Solanum lycopersicum (Sl) and its wild relatives S. pennellii (Spe) and S. pimpinellifolium (Spi) were analyzed using bioinformatics approaches. In total, 238, 202 and 217 NB-LRR genes of 8 different types were found in Sl, Spe and Spi, respectively. The three species showed similar genomic characteristics. The NB-LRR genes were mainly distributed on chromosomes 4, 5 and 11 and located at the distal zones, forming multiple clusters and tandem duplicates. A large number of homologs appeared through gene expansion, with most Ka/Ks values being less than 1, indicating that purifying selection had occurred in evolution. These genes were mainly expressed in root and could respond to different biotic stresses. RT-qPCR analysis revealed that SlNLR genes could respond to tomato bacterial wilt, with SlNLR1 probably involved in the resistance response, whereas others being the opposite. The transcription factors (TFs) and interaction proteins that regulate target genes were mainly Dof, NAC and MYB families and kinases. The results provide a basis for the isolation and application of related genes in plant disease resistance breeding.

Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms

Plant bacterial and fungal diseases cause significant agricultural losses and need to be controlled. Beneficial bacteria are promising candidates for controlling these diseases. In this study, Streptomyces sp. JCK-6131 exhibited broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In vitro assays showed that the fermentation filtrate of JCK-6131 inhibited the growth of bacteria and fungi with minimum concentration inhibitory (MIC) values of 0.31–10% and 0.31–1.25%, respectively. In the in vivo experiments, treatment with JCK-6131 effectively suppressed the development of apple fire blight, tomato bacterial wilt, and cucumber Fusarium wilt in a dose-dependent manner. RP-HPLC and ESI-MS/MS analyses indicated that JCK-6131 can produce several antimicrobial compounds, three of which were identified as streptothricin E acid, streptothricin D, and 12-carbamoyl streptothricin D. In addition, the disease control efficacy of the foliar application of JCK-6131 against tomato bacterial wilt was similar to that of the soil drench application, indicating that JCK-6131 could enhance defense resistance in plants. Molecular studies on tomato plants showed that JCK-6131 treatment induced the expression of the pathogenesis-related (PR) genes PR1, PR3, PR5, and PR12, suggesting the simultaneous activation of the salicylate (SA) and jasmonate (JA) signaling pathways. The transcription levels of PR genes increased earlier and were higher in treated plants than in untreated plants following Ralstonia solanacearum infection. These results indicate that Streptomyces sp. JCK-6131 can effectively control various plant bacterial and fungal diseases via two distinct mechanisms of antibiosis and induced resistance.

The inhibitory effects of different types of Brassica seed meals on the virulence of Ralstonia solanacearum.

Understanding the specific inhibitory effects of different Brassica seed meals (BSMs) on soilborne pathogens is important for their application as biocontrol agents for controlling plant disease. In this study, the seed meals of Brassica napus L. (BnSM), Brassica campestris L. (BcSM), and Brassica juncea L. (BjSM), and the combined seed meal of BcSM and BjSM (CSM, 1:1), were selected for investigation. The inhibitory effects of these seed meals on the plant pathogen Ralstonia solanacearum (Smith) and tomato bacterial wilt, were assessed and compared. All the BSMs significantly inhibited the growth of R. solanacearum in vitro. Furthermore, the BSMs could effectively suppress R. solanacearum virulence traits, including motility, exopolysaccharide production, dehydrogenase activity, virulence-related gene expression, and colonization in the soil. Among them, BjSM showed the best inhibiting effects, and CSM displayed synergic toxicity against R. solanacearum. In addition, the predominant antibacterial compounds in BcSM and BjSM were identified as the volatile compounds, 3-butenyl isothiocyanate and allyl isothiocyanate, respectively. Finally, pot experiment verified that the control effects of BjSM and CSM on tomato wilt reached more than 90%. This is the first study to report on the ability of different kinds of BSMs to suppress the virulence of R. solanacearum and biocontrol efficiencies against bacterial wilt in tomato plants. Furtherly, the main antibacterial compounds in the BSMs were identified. The results demonstrated that CSM may possess potential for controlling bacterial wilt caused by R. solanacearum. The results provide a fresh perspective for comprehending the mechanism underlying BSM suppression of pathogens and plant disease.

Wheat straw biochar amendment suppresses tomato bacterial wilt caused by Ralstonia solanacearum: Potential effects of rhizosphere organic acids and amino acids

Complex interactions based on host plant, rhizosphere microorganisms and soil microenvironment are presumed to be responsible for the suppressive properties of biochar against soil-borne diseases, although the underlying mechanisms are not well understood. This study is designed to evaluate the efficacy of biochar amendment for controlling tomato bacterial wilt caused by Ralstonia solanacearum, and to explore the interactions between biochar-induced changes in rhizosphere compound composition, the pathogen and tomato growth. The results showed that biochar amendment decreased disease incidence by 61–78% and simultaneously improved plant growth. The positive ‘biochar effect’ could be associated with enhanced microbial activity and alterations in the rhizosphere organic acid and amino acid composition. Specifically, elevated rhizosphere citric acid and lysine, but reduced salicylic acid, were induced by biochar which improved microbial activity and rendered the rhizosphere unsuitable for the development of R. solanacearum. In addition, nutrients which were either made more available by the stimulated microbial activity or supplied by the biochar could improve plant vigor and potentially enhance tomato resistance to diseases. Our findings highlight that biochar's ability to control tomato bacterial wilt could be associated with the alteration of the rhizosphere organic acid and amino acid composition, however, further research is required to verify these ‘biochar effects’ in field conditions.

N-acetylcysteine, a biofilm disruptor, formulated as a layered nanomaterial for the control of bacterial wilt in tomato

ISHS VI International Symposium on Tomato Diseases: Managing Tomato Diseases in the Face of Globalization and Climate Change N-acetylcysteine, a biofilm disruptor, formulated as a layered nanomaterial for the control of bacterial wilt in tomato

English

Ralstonia solanacearum causes bacterial wilt of tomato and limits the crop production, and antagonistic microorganisms use to suppress the disease, of which Trichoderma and Pseudomonas species are the most effective agents to control bacterial wilt. In the present study, attempt was made to isolate these two microorganisms to evaluate their effectiveness to control R. solanacearum, the causal agent of bacterial wilt disease of tomato under greenhouse conditions. Thus R. solanacearum, Pseudomonas and Trichoderma spp. were isolated from wilted and healthy tomato plants grown from farmer's field in Ziway and Meki, Oromia Ethiopia. The virulence of the pathogen and the antagonistic effect of the bacteria and fungi were evaluated against R. solanacearum in vitro and in vivo condition. Based on the in vitro results the best two isolates were selected to show their antagonistic effect under greenhouse condition in single and combined designs. The result showed the pathogenicity test of the isolates were evaluated under greenhouse condition, and isolate AAURS1 showed highest virulence (75%) followed by isolate APPRCRS2 with pathogenicity of 50%. With regard to antagonism test, isolates AAURB20 and AAUTR23 showed the highest inhibition against R. solanacearum with inhibition zone of 16 and 15 mm, respectively. Among the treatments co-inoculation was more effective and reduced disease incidence by 13.33% and increased the bio-control efficacy by 72.22% when compared with individual treatment and negative control. The isolates significantly increased the plant height and dry weight by 72.33 cm and 12.18 g, respectively. Thus, the combined use of the biocontrol agents significantly reduced the incidence of tomato bacterial wilt disease. However, their performance should be evaluated using other yield parameters under field conditions to produce healthy tomato seedling to minimize the use of chemicals and reduce  environmental pollution.   Key words: Biocontrol, Pseudomonas, Ralstonia solanacearum, Trichoderma.

Control of tomato bacterial wilt using resistant rootstocks with soil disinfection treatments

Control of tomato bacterial wilt using resistant rootstocks with soil disinfection treatments

Unravelling physiological signatures of tomato bacterial wilt and xylem metabolites exploited by Ralstonia solanacearum.

The plant pathogen Ralstonia solanacearum uses plant resources to intensely proliferate in xylem vessels and provoke plant wilting. We combined automatic phenotyping and tissue/xylem quantitative metabolomics of infected tomato plants to decipher the dynamics of bacterial wilt. Daily acquisition of physiological parameters such as transpiration and growth were performed. Measurements allowed us to identify a tipping point in bacterial wilt dynamics. At this tipping point, the reached bacterial density brutally disrupts plant physiology and rapidly induces its death. We compared the metabolic and physiological signatures of the infection with drought stress, and found that similar changes occur. Quantitative dynamics of xylem content enabled us to identify glutamine (and asparagine) as primary resources R. solanacearum consumed during its colonization phase. An abundant production of putrescine was also observed during the infection process and was strongly correlated with in planta bacterial growth. Dynamic profiling of xylem metabolites confirmed that glutamine is the favoured substrate of R. solanacearum. On the other hand, a triple mutant strain unable to metabolize glucose, sucrose and fructose appears to be only weakly reduced for in planta growth and pathogenicity.

Genome analysis provides insights into the biocontrol ability of Mitsuaria sp. strain TWR114.

Mitsuaria sp. TWR114 is a biocontrol agent against tomato bacterial wilt (TBW). We aimed to gain genomic insights relevant to the biocontrol mechanisms and colonization ability of this strain. The draft genome size was found to be 5,632,523bp, with a GC content of 69.5%, assembled into 1144 scaffolds. Genome annotation predicted a total of 4675 protein coding sequences (CDSs), 914 pseudogenes, 49 transfer RNAs, 3 noncoding RNAs, and 2 ribosomal RNAs. Genome analysis identified multiple CDSs associated with various pathways for the metabolism and transport of amino acids and carbohydrates, motility and chemotactic capacities, protection against stresses (oxidative, antibiotic, and phage), production of secondary metabolites, peptidases, quorum-quenching enzymes, and indole-3-acetic acid, as well as protein secretion systems and their related appendages. The genome resource will extend our understanding of the genomic features related to TWR114's biocontrol and colonization abilities and facilitate its development as a new biopesticide against TBW.

Characterization and diversity of Indian isolates of Ralstonia solanacearum inciting bacterial wilt of tomato

Tomato (Solanum lycopersicum L.) a native of South America is a source of vitamins, minerals and antioxidant lycopene, and considered as protective food. In India, the crop is grown under the area of about 0.458 M ha with production of 7.277 M mt and productivity of 15.9 mt/ha. Biotic factors play a role in low productivity of the crops in India. Among various diseases, bacterial wilt disease incited by Ralstonia solanacearum (Smith) Yabuuchi et al. (1996) is a devastating bacterium causing damage to the crop ranging from 2 to 95% depending on the seasons and cultivars. In India, majority of the isolates of R. solanacearum infecting tomato crop belonged to biovar 3, race 1 and phylotype I. However, less than 10% isolates of the bacteria belonged to biovar 4 particularly in the states of Jharkhand and Himachal Pradesh. The genetic diversity of R. solanacearum has been studied by using methods such as random amplified polymorphic DNA (RAPD), PCR—RFLP (PCR—Restriction Length Fragment Polymorphism) of hrp gene, Repetitive element palindromic-polymerase chain reaction (Rep-PCR) and multilocus sequence typing (MLST). The genetic diversity was recorded in Indian R. solanacearum isolates irrespective of their occurrence in different agro-climatic conditions.

Effects of Combined Application of Potassium Silicate and Salicylic Acid on the Defense Response of Hydroponically Grown Tomato Plants to Ralstonia solanacearum Infection

Bacterial wilt, caused by soilborne pathogenic bacterium Ralstonia solanacearum, is a serious and widespread disease that affects global tomato production. Both silicon (Si) and salicylic acid (SA) play important roles in enhancing tomato resistance against bacterial wilt, however, their combined effects on the defense responses of infected tomato plants remain unknown. Hence, the combined effects of Si and SA on physiological and biochemical parameters of R. solanacearum-infected tomato plants were investigated. The combination treatment of Si and SA significantly decreased disease incidences, lipoxygenase (LOX) activity and ethylene (ET) production. The combined treatments were more prominent in improving the morphological traits of root systems, such as root length, root surface area, average root diameter and root volume. The activities of polyphenol oxidase (PPO) and peroxidase (POD) and the concentrations of total soluble phenolics (TSPs) and lignin-thioglycolic acid (LTGA) derivatives were significantly increased in the plants with combined treatments. Si in combination with SA could significantly enhance neutral invertase (NI) and acid invertases (AI) activities in the leaves of tomato plants at 3 days post-infection (dpi) compared with application of Si alone. Three defense-related genes, PAL, POD and pathogenesis-related protein 1 (PR1), were significantly induced in Si+SA treatment at 7 dpi when compared with individual application of Si or SA. The expression level of salicylic acid-binding protein 2 (SABP2) was significantly higher for combination treatment when compared with treatment of Si or SA alone. The possible mechanisms involved in the synergistic effects of Si and SA on the control of tomato bacterial wilt were proposed. This study indicates that under hypertonic conditions, the combined application of 2.0 mM potassium silicate (K2SiO3) and 0.5 mM SA had a synergistic effect on the control of tomato bacterial wilt.

Farklı Nitrifikasyon İnhibitörlerinin Domateste Bakteriyel Kanser ve Solgunluk (Clavibacter Michiganensis Subsp. Michiganensis) Hastalığına Etkisi

Domateste bakteriyel kanser ve solgunluk hastalığı ekonomik boyutta bitki gelişimini ve verimini sınırlandıran önemli bir hastalıktır. Hastalığa dayanıklı ticari çeşit bulunmaması ve kimyasal mücadelenin yetersiz olması nedeniyle mücadelesi oldukça zordur. Bu çalışmanın amaçları (1) domates bitkilerinden farklı yıllarda elde edilen Cmm izolatlarının klasik ve moleküler yöntemlerle tanısını yapmak, (2) domates bakteriyel kanser ve solgunluk hastalığına karşı farklı nitrifikasyon inhibitörlü gübrelerin hastalık gelişimi üzerine etkisini incelemektir.Elde edilen sekiz adet izolat Mirabilis jalapa yapraklarında aşırı duyarlılık, domates fidelerinde patojenite özelliği ve agaroz jelde 614 bp bant oluşturarak Cmm olarak tanılanmışlardır. Saksı denemeleri şeklinde yürütülen bu çalışmada karşılaştırma olarak azot inhibitörsüz normal amonyum sülfat ile yavaş salınım özelliğine sahip disiyandiamid (DCD) ve 3,5 dimetilpirazolyum gliseroborat (DMPB) azot inhibitörlü amonyum sülfat gübreleri kullanılmıştır. Denemede gübrelerin 100, 250 ve 500 ppm dozları bitkilere sulama suyu şeklinde birer hafta arayla üç kez uygulanmış ve uygulanan gübrelerin etkinliği, pozitif kontrol grubundaki bitkilerle kıyaslanarak belirlenmiştir. İki kez tekrarlanan çalışmada, birinci denemede gübreler hastalık gelişimini %42-78 oranları arasında, ikinci denemede ise %44-82 oranları arasında engellemiştir. Her iki denemede inhibitörlü ve inhibitörsüz gübrelerin 500 ppm dozları hastalığı baskılamada en etkili uygulamalar olmuştur. Bu çalışma azot inhibitörlü gübrelerin bakteriyel kanser ve solgunluk hastalığı üzerine etkisini ortaya koyan ilk çalışmadır. Sonuç olarak, domates bakteriyel kanser ve solgunluk hastalığı ile entegre mücadele programlarına inhibitörlü gübrelerin de eklenmesi faydalı olacaktır.

Effect of compost amendments from Tridax procumbens L. and cow dung on the microbial activity and agronomic traits of tomato plants infested with Ralstonia solanacearum

Incorporation of compost amendments into the soil is a promising strategy for the control of some soil-borne plant diseases. The aim of this study was to evaluate the effect of compost amendments from Tridax procumbens L. and cow dung on the microbial activity and agronomic traits of tomato plants infested with Ralstonia solanacearum. Tridax procumbens L. and cow dung were composted for 20 days using five different proportions. Physicochemical properties and microbial load of the composts were determined using the standard methods. Isolates were identified based on morphological, colonial and biochemical characterisations. Different compost concentrations were added to sterile soil with and without Ralstonia solanacearum after which tomato nurseries were transplanted. Agronomic parameters such as plant height, number of leaves, stem girth and leave area were determined after two and four weeks of transplanting. Cow dung only (CD) had the highest Total Bacterial Count (TBC) and Total Fungal Count (TFC) of 265.67 x 104 CFU/g and 92.3 x 103 CFU/g respectively while T. procumbens only (TP) had the least TBC (46.3 x 104 CFU/g) and TFC (13.33 x 103 CFU/g) . Bacillus species, Pseudomonas species, Aspergillus species and Penicillium sp. were predominant throughout the duration of composting. Highest pH (7.3) was recorded by Cow dung + T. procumbens (C:T)(1:1) while TP had the least pH (6.2). Highest temperature (41.9 °C) was recorded by C:T(1:2) while TP recorded the least 29.8 °C. After four weeks, the compost amendments improved the plant height, number of leaves, stem girth, and leaf area in the un-infested and diseased tomato plants. This study showed that compost from T. procumbens and cow dung can be used to improve the growth of tomato and also suppress bacterial wilt of tomato.

Biological control of bacterial wilt in tomato through the metabolites produced by the biocontrol fungus, Trichoderma harzianum

BackgroundRalstonia solanacearum causes bacterial wilt disease in tomato and other crops resulting in huge economic losses worldwide. Several measures have been explored for the control of R. solanacearum, but the desired control level of the disease through sustainable and ecofriendly way is still awaited.Main bodyIn this study, fungal metabolites produced by Trichoderma harzianum were investigated in the form of crude extract for the management of R. solanacearum both in vitro and in planta in tomato plants. In in vitro investigation, fungal metabolites were checked for their antibacterial potential at different concentrations (30, 60, 90, 120, 150, and 180 mg ml−1) and bacterial cell morphology was observed under scanning electron microscopy (SEM). In a greenhouse experiment, different application times (0, 3, and 6 days before transplantation DBT) and doses (0, 3, 6, and 9%) of the fungal metabolites were tested for their effects on soil bacterial population, disease severity and plant growth of tomato plants. The in vitro evaluation showed a strong antibacterial activity of fungal metabolites in concentration dependent manner. The highest concentration 180 mg ml−1 produced maximum inhibition zone (20.2 mm) having non-significant difference with the inhibition zone (20.5 mm) produced by the standard antibiotic streptomycin. The SEM analysis revealed severe morphological destructions of bacterial cells. In case of greenhouse experiment, the highest decrease in soil bacterial population, lowest disease severity, and maximum increase in plant growth parameters were obtained by highest dose (9%) and longest application time (6 DBT).ConclusionThe fungal metabolites produced by T. harzianum could be used as low-cost, environment-friendly, and sustainable management strategy for the control of R. solanacearum in tomato plants.

Evaluation of the Efficacy of Trichoderma and Pseudomonas Species against Bacterial Wilt (Ralstonia Isolates) of Tomato (Lycopersicum Spp.)

Ralstonia solanacearum causes bacterial wilt of tomato and limits the crop production, and antagonistic microorganisms such as fungi and bacteria are used to suppress the disease, of which Trichoderma spp. and Pseudomonas spp. are the most effective agents to control bacterial wilt of various horticultural and other crops. In the present study, attempt was made to isolate these two microorganisms to evaluate their effectiveness to control R. solanacearum the causal agent of bacterial wilt disease of tomato under greenhouse conditions. Thus R. solanacearum, Pseudomonas and Trichoderma spp. were isolated from wilted and healthy tomato plants grown from farmer's field in Ziway and Meki. The virulence of the pathogen and the antagonistic effect of the bacteria and fungi were evaluated against R. solanacearum in vitro and in vivo condition. Based on the in vitro results the best two isolates were selected to show their antagonistic effect under greenhouse condition in single and combined designs. The result showed the pathogenicity test of the isolates were evaluated under greenhouse condition, and isolate AAURS1 showed highest virulence (75%) followed by isolate APPRCRS2 with pathogenicity of 50%. With regard to antagonism test, isolates AAURB20 and AAUTR23 showed the highest inhibition against R. solanacearum with inhibition zone of 16 mm and 15 mm, respectively. Among the treatments co-inoculation (AAURB20+AAUTR23) was more effective and reduced disease incidence by 13.33% and increased the bio-control efficacy by 72.22% when compared with individual treatment and negative control (Un inoculated treatment). The isolates significantly increased the plant height and dry weight by 72.33 cm, and 12.18 g, respectively. Thus, the combined use of the biocontrol agents significantly reduced the incidence of tomato bacterial wilt disease. However, their performance should be evaluated using other yield parameters under field conditions to produce healthy tomato seedling to minimize the use of chemicals and reduce environmental pollution.

Nitrogen-doped carbon dots alleviate the damage from tomato bacterial wilt syndrome: systemic acquired resistance activation and reactive oxygen species scavenging

Nitrogen doped carbon dots suppressed bacterial wilt in tomato through photosynthesis modulation, ROS scavenging, and resistance activation.

Mixtures of Suppressive Bacteria Enhance Biological Control of Tomato Bacterial Wilt

Mixtures of Suppressive Bacteria Enhance Biological Control of Tomato Bacterial Wilt