Abstract
BackgroundRalstonia solanacearum causes bacterial wilt disease in tomato and other crops resulting in huge economic losses worldwide. Several measures have been explored for the control of R. solanacearum, but the desired control level of the disease through sustainable and ecofriendly way is still awaited.Main bodyIn this study, fungal metabolites produced by Trichoderma harzianum were investigated in the form of crude extract for the management of R. solanacearum both in vitro and in planta in tomato plants. In in vitro investigation, fungal metabolites were checked for their antibacterial potential at different concentrations (30, 60, 90, 120, 150, and 180 mg ml−1) and bacterial cell morphology was observed under scanning electron microscopy (SEM). In a greenhouse experiment, different application times (0, 3, and 6 days before transplantation DBT) and doses (0, 3, 6, and 9%) of the fungal metabolites were tested for their effects on soil bacterial population, disease severity and plant growth of tomato plants. The in vitro evaluation showed a strong antibacterial activity of fungal metabolites in concentration dependent manner. The highest concentration 180 mg ml−1 produced maximum inhibition zone (20.2 mm) having non-significant difference with the inhibition zone (20.5 mm) produced by the standard antibiotic streptomycin. The SEM analysis revealed severe morphological destructions of bacterial cells. In case of greenhouse experiment, the highest decrease in soil bacterial population, lowest disease severity, and maximum increase in plant growth parameters were obtained by highest dose (9%) and longest application time (6 DBT).ConclusionThe fungal metabolites produced by T. harzianum could be used as low-cost, environment-friendly, and sustainable management strategy for the control of R. solanacearum in tomato plants.
Highlights
Ralstonia solanacearum causes bacterial wilt disease in tomato and other crops resulting in huge economic losses worldwide
This study aimed to find the potential of fungal metabolites of T. harzianum for the management of bacterial wilt disease in tomato plants both in vitro and in planta
The inhibition zone produced by highest concentration of fungal extract was similar to the inhibition zone (20.5 mm) produced by the standard antibiotic streptomycin used as a positive control
Summary
Ralstonia solanacearum causes bacterial wilt disease in tomato and other crops resulting in huge economic losses worldwide. Different application times (0, 3, and 6 days before transplantation DBT) and doses (0, 3, 6, and 9%) of the fungal metabolites were tested for their effects on soil bacterial population, disease severity and plant growth of tomato plants. Ralstonia solanacearum is a G- bacterium, which is considered as one of the highly destructive pathogens It is distributed worldwide and has significant economic impact globally (Scherf et al 2010). It is a pathogen of many plants, causes wilting disease and especially more destructive to tomato plants, leading to substantial losses in tropical and other regions with warm temperature (Huet 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
