Bacterial Types Research Articles

Survey the bacterial types in breast tissue and CA13-5 for women with breast disease in Babylon province

Breast tumor may be malignant and benign so these tumor and breast diseases are harboring bacteria ,but types of bacteria may differ among breast tumors so the current study was aimed to detect type of bacteria in breast tissue . Specimens( Breast tissue and blood) was collected from 50 female (aged ranged between 14 to 60 ) undergoing breast surgery at AL-Hilla Teaching Hospital and Al-Sadiq Hospital in Babylon Province during period from November 2021 - March 2022 and blood sample also collected from healthy women as control . tissue obtained was combined outside the marginal zone, approximately 5 cm away from the neoplasm. After enucleation, the new tissue was immediately placed in a sterile plane tube or urine cup it contain a normal saline solution and it was cut , homogenized by using a sterile surgical scalpel and wooden sticks within 30 min of collection.2 it was cultivated on blood agar &MacConkey media ,and then on selective media .and Identification for bacteria by microscopic ,biochemical test and confirmed by Vitec 2Compact System . The results appeared the percentage of malignant tumor (17.14%) and benign (82.85%) , The growth percentage (88.57%) while no growth was (11.42%).

Bacterial Enoyl-Reductases: The Ever-Growing List of Fabs, Their Mechanisms and Inhibition.

Enoyl-ACP reductases (ENRs) are enzymes that catalyze the last step of the elongation cycle during fatty acid synthesis. In recent years, new bacterial ENR types were discovered, some of them with structures and mechanisms that differ from the canonical bacterial FabI enzymes. Here, we briefly review the diversity of structural and catalytic properties of the canonical FabI and the new FabK, FabV, FabL, and novel ENRs identified in a soil metagenome study. We also highlight recent efforts to use the newly discovered Fabs as targets for drug development and consider the complex evolutionary history of this diverse set of bacterial ENRs.

Localized electrical stimulation triggers cell-type-specific proliferation in biofilms.

Biological systems ranging from bacteria to mammals utilize electrochemical signaling. Although artificial electrochemical signals have been utilized to characterize neural tissue responses, the effects of such stimuli on non-neural systems remain unclear. To pursue this question, we developed an experimental platform that combines a microfluidic chip with a multielectrode array (MiCMA) to enable localized electrochemical stimulation of bacterial biofilms. The device also allows for the simultaneous measurement of the physiological response within the biofilm with single-cell resolution. We find that the stimulation of an electrode locally changes the ratio of the two major cell types comprising Bacillus subtilis biofilms, namely motile and extracellular-matrix-producing cells. Specifically, stimulation promotes the proliferation of motile cells but not matrix cells, even though these two cell types are genetically identical and reside in the same microenvironment. Our work thus reveals that an electronic interface can selectively target bacterial cell types, enabling the control of biofilm composition and development.

Interactive relationship between Trp metabolites and gut microbiota: The impact on human pathology of disease.

Tryptophan (Trp), an α-amino acid, is the precursor of serotonin (5-hydroxytryptamine, 5-HT), which is involved in a variety of features of metabolic function and human nutrition. Evidence highlights the role of Trp metabolites (exclusively 5-HT) in the gastrointestinal (GI) tract; however, the mechanisms of action involved in the release of 5-HT in the GI tract are still unknown. Considering the fact that variations of 5-HT may facilitate the growth of certain GI disorders, gaining a better understanding of the function and release of 5-HT in the GI tract would be beneficial. Additionally, investigating Trp metabolism may clarify the relationship between Trp and gut microbiota. It is believed that other metabolites of Trp (mostly that of the kynurenine pathway) may play a significant role in controlling gut microbiota function. In this review, we have attempted to summarize the current research investigating the relationship of gut microbiota, Trp and 5-HT metabolism (with particular attention paid to their metabolite type, as well as a discussion of the research methods used in each study). Taking together, regarding the role that Trp/5-HT plays in a range of physical and mental diseases, the gut bacterial types, as well as the related disorders, have been exclusively considered.

E.coli biofilm formation and its susceptibility towards T4 bacteriophages studied in a continuously operating mixing - tubular bioreactor system.

SummaryA system consisting of a connected mixed and tubular bioreactor was designed to study bacterial biofilm formation and the effect of its exposure to bacteriophages under different experimental conditions. The bacterial biofilm inside silicone tubular bioreactor was formed during the continuous pumping of bacterial cells at a constant physiological state for 2 h and subsequent washing with a buffer for 24 h. Monitoring bacterial and bacteriophage concentration along the tubular bioreactor was performed via a piercing method. The presence of biofilm and planktonic cells was demonstrated by combining the piercing method, measurement of planktonic cell concentration at the tubular bioreactor outlet, and optical microscopy. The planktonic cell formation rate was found to be 8.95 × 10−3 h−1 and increased approximately four‐fold (4×) after biofilm exposure to an LB medium. Exposure of bacterial biofilm to bacteriophages in the LB medium resulted in a rapid decrease of biofilm and planktonic cell concentration, to below the detection limit within < 2 h. When bacteriophages were supplied in the buffer, only a moderate decrease in the concentration of both bacterial cell types was observed. After biofilm washing with buffer to remove unadsorbed bacteriophages, its exposure to the LB medium (without bacteriophages) resulted in a rapid decrease in bacterial concentration: again below the detection limit in < 2 h.

Structure Revision of Penipacids A–E Reveals a Putative New Cryptic Natural Product, N-aminoanthranilic Acid, with Potential as a Transcriptional Regulator of Silent Secondary Metabolism

Reconsideration of the spectroscopic data for penipacids A–E, first reported in 2013 as the acyclic amidines 1–5 from the South China deep sea sediment-derived fungus Penicillium paneum SD-44, prompted a total synthesis structure revision as the hydrazones 6–10. This revision strongly supported the proposition that penipacids A–B (6–7) were artifact Schiff base adducts of the cryptic (undetected) natural product N-aminoanthranilic acid (11) with diacetone alcohol, induced by excessive exposure to acetone and methanol under acidic handling conditions. Likewise, the revised structures for penipacids C–D (8–9) and E (10) raise the possibility that they may also be artifact Schiff base adducts of 11 and the media constituents pyruvic acid and furfural, respectively. A review of the natural products literature revealed other Schiff base (hydrazone) natural products that might also be viewed as Schiff base adduct artifacts of 11. Having raised the prospect that 11 is an undetected and reactive cryptic natural product, we went on to establish that 11 is not cytotoxic to a range of bacterial, fungal or mammalian (human) cell types. Instead, when added as a supplement to microbial cultivations, 11 can act as a chemical cue/transcriptional regulator, activating and/or enhancing the yield of biosynthetic gene clusters encoding for other natural product chemical defenses. This study demonstrates the value of challenging the structure and artifact status of natural products, as a window into the hidden world of cryptic and highly reactive natural products.

Dual-Approach Electrochemical Surface-Enhanced Raman Scattering Detection of Mycobacterium tuberculosis in Patient-Derived Biological Specimens: Proof of Concept for a Generalizable Method to Detect and Identify Bacterial Pathogens.

The recent surge in infectious disease-causing pathogens, resulting in global catastrophe, has merited a pivotal quest toward point-of-care (POC) diagnostics. Mycobacterium tuberculosis (MTB) is still the top bacterium-based infectious disease-causing pathogen worldwide. In a concerted effort toward simplifying and decentralizing the discriminatory screening of MTB causing pathogens, electrochemical surface-enhanced Raman scattering (EC-SERS) was adopted to create a customized screening tool. The development strategy combined five key factors, including (i) a simplified Tollens'-based chemical synthesis method for bulk supply of silver nanoparticles, (ii) the deliberate surface modification of nanoparticles with carefully selected polyelectrolytes to resemble the conditioning layer usually found on a natural substratum, (iii) uniform SERS-active films formed through simple unprogrammed assembly, (iv) the controlled manipulation of the local electric field through applied voltage using a technique that does not conform to the limitations of classical EC-SERS, and (v) the inherent specificity of the target-specific SERS vibrational signature. The EC-SERS platform was able to discriminatively detect and identify TB-derived mycobacteria, including three clinically relevant MTB strains, TB-H37Rv, TB-HN878, and TB-CDC1551. Moreover, a customized voltage stepping protocol, compatible with either the inclusion of a short preincubation step or with in situ EC-SERS is illustrated. From the obtained SERS vibrational signatures, a band indicating a mode unique to TB-derived/TB-affiliated mycobacteria and thus not observed for other bacterial types used in this study was illustrated. Furthermore, provisional investigation, done as prelude for assessing the potential for translational adaptability of the EC-SERS technique toward POC clinical settings for sputum and urine specimens, was carried out.

MP49-20 UROTYPES IN YOUNG WOMEN WITH PELVIC PAIN: A PILOT ANALYSIS OF CLINICAL URINARY MICROBIOME CLASSIFICATION

You have accessJournal of UrologyCME1 May 2022MP49-20 UROTYPES IN YOUNG WOMEN WITH PELVIC PAIN: A PILOT ANALYSIS OF CLINICAL URINARY MICROBIOME CLASSIFICATION James M. Weinberger, L. Marcella Henao, Crystal Cisneros, James E. Ackerman, and A. Lenore Ackerman James M. WeinbergerJames M. Weinberger More articles by this author , L. Marcella HenaoL. Marcella Henao More articles by this author , Crystal CisnerosCrystal Cisneros More articles by this author , James E. AckermanJames E. Ackerman More articles by this author , and A. Lenore AckermanA. Lenore Ackerman More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002624.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: There are substantial differences in the urobiomes of women depending on age and menopausal status. Our previous work demonstrated that younger women (age <45) tended to have a more limited set of dominant microbes and could be classified by Next Generation Sequencing into one of 3 “urotypes” based on the composition of Lactobacilli and E. coli. We sought to determine if we could confirm that pattern in an unselected sampling of women seeking urogynecologic care and if these patterns correlated with presenting complaints. METHODS: Women (age 18-45) presenting with LUTS were prospectively enrolled if their clean-catch urine sample was deemed unnecessary for analysis by the provider. Samples were assessed via quantitative PCR (qPCR) for E. coli and Lactobacillus crispatus, gasseri, iners and jensenii and classified into urotypes using unsupervised clustering. The subset of women complaining of painful urogenital conditions were classified into the following categories using ICD-10 codes and primary provider-given diagnosis: bladder-specific pain syndrome (BPS), myofascial pelvic pain (MPP), and unspecified pelvic pain (PP). We performed correlations between bacterial types and pain diagnoses. RESULTS: Using Lactobacillus spp. and E. coli, premenopausal women could be classified into 3 dominant urotypes; the majority of asymptomatic subjects fell into Urotype 1(low Lactobacilli, low E. coli). Of the 186 premenopausal women enrolled, 56 were diagnosed with BPS, MPP, or PP. Examining across urotype, the MPP and BPS groups segregate; patients with MPP were Urotype 1, while BPS exhibited elevated L. iners (Urotype 2) (Figure 1). The majority of PP patients were Lactobacillus-poor, falling into the E.coli-positive urotype (Urotype 3). CONCLUSIONS: Pelvic pain is a common complaint in this age group. While previous studies of pelvic pain have noted differences in diversity and Lactobacilli abundance between controls and pain groups, our qPCR-based urotyping was able to classify young women into three subgroups, demonstrating differential associations with asymptomatic controls and pelvic pain phenotypes. These data suggest that urotype data may be a meaningful mechanism to subclassify pelvic pain patients. Future studies are needed to determine if these groups correlate to treatment responses. Source of Funding: N/a © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e862 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information James M. Weinberger More articles by this author L. Marcella Henao More articles by this author Crystal Cisneros More articles by this author James E. Ackerman More articles by this author A. Lenore Ackerman More articles by this author Expand All Advertisement PDF DownloadLoading ...

Structural Elucidation and genetic identification of the O-antigen from a novel serogroup of Escherichia coli strain 2017LL031

The O-antigen is an important virulence factor involved in the survival, virulence and invasion of bacteria. The bacterial serological types are highly dependent on these surface-exposed and structurally unique O-antigen structures. In this work, the structure of O-antigen from an Escherichia coli strain 2017LL031 was elucidated as a hexasaccharide repeating unit: →3)-[β-D-Glcp-(1 → 2)]-α-L-Rhap-(1 → 3)-[α-D-Quip-(1 → 3)-α-D-GlcpA-(1 → 2)]-β-L-Fucp-(1 → 3)-β-D-GlcpNAc-(1→, which is completely different from all known E. coli serogroups. The O-antigen gene cluster (O-AGC) of 2017LL031 was also analyzed and correlates well to its O–Ag. Moreover, the O-AGC of 2017LL031 was deleted and its role in O–Ag biosynthesis was confirmed experimentally. Taken together, our results present that a novel E.coli serotype 2017LL031 is identified.

Association of Conjunctivitis through Microflora in Optical Lenses Users

Aim: To find the association of conjunctivitis through microflora in optical lenses users. Study design: Cross sectional study Place and duration of study: Department of Ophthalmology, Pakistan Railways Hospital, Islamic International Medical College, Rawalpindi from 1st January 2021 to 30th June 2021. Methodology: Forty patients were enrolled. Patient’s clinical symptoms, features were examined for conjunctivitis and slit lamp examination was also followed. Swab cultures from optical lenses were taken for conducting various agar v=based cultures for identifying microorganisms presence. Results: There were more males than females with mean age were 25±4.5 years. Clinical examination presented dryness in eyes as highest percentage. The anti-microbial sensitivity showed highest susceptibility pattern for resistance of ampicillin. Various bacterial types showed that highest frequency of Micrococci as 47.5%. Conclusion: A Robust association of conjunctivitis through microflora was found optical lenses users. Key words: Hygiene practices; Microflora; Optical lens users; Conjunctivitis

BMC Caller: a webtool to identify and analyze bacterial microcompartment types in sequence data

Bacterial microcompartments (BMCs) are protein-based organelles found across the bacterial tree of life. They consist of a shell, made of proteins that oligomerize into hexagonally and pentagonally shaped building blocks, that surrounds enzymes constituting a segment of a metabolic pathway. The proteins of the shell are unique to BMCs. They also provide selective permeability; this selectivity is dictated by the requirements of their cargo enzymes. We have recently surveyed the wealth of different BMC types and their occurrence in all available genome sequence data by analyzing and categorizing their components found in chromosomal loci using HMM (Hidden Markov Model) protein profiles. To make this a “do-it yourself” analysis for the public we have devised a webserver, BMC Caller (https://bmc-caller.prl.msu.edu), that compares user input sequences to our HMM profiles, creates a BMC locus visualization, and defines the functional type of BMC, if known. Shell proteins in the input sequence data are also classified according to our function-agnostic naming system and there are links to similar proteins in our database as well as an external link to a structure prediction website to easily generate structural models of the shell proteins, which facilitates understanding permeability properties of the shell. Additionally, the BMC Caller website contains a wealth of information on previously analyzed BMC loci with links to detailed data for each BMC protein and phylogenetic information on the BMC shell proteins. Our tools greatly facilitate BMC type identification to provide the user information about the associated organism’s metabolism and enable discovery of new BMC types by providing a reference database of all currently known examples.

Specific alterations in mucosa-associated bacterial composition in ulcerative colitis (UC) patients with different degrees of inflammation

The aim of this study is to evaluate the specific bacterial communities and alterations and to explore the method predicting the microbiological evolution tendency of ulcerative colitis (UC) patients. A total of 29 UC patients and 18 healthy subjects (HS) were enrolled. UC patients were grouped into mild, moderate and severe UC based on the Mayo Score. The bacterial types of the groups were distinctly different with each other on different classification levels. Interestingly, the bacterial composition of severe UC was distinctly different from others. The Chao and Ace indices of bacterial microbiota were significantly higher in the severe UC group. The main phyla in UC and HS were Firmicutes, Bacteroidetes and Proteobacteria. The percentage of the 3 main phyla in severe UC was lower than that in non-severe UC. Similar to the other groups, the main genera in severe UC were Bacteroides and Escherichia-Shigella. However, Parabacteroides and a type of Prevotella disappeared in the severe UC group. Some types, such as Faecalibacterium and Fusobacterium, decreased. On the contrary, the other types increased. The abundance of the putative pathogenic bacteria in mild UC, moderate UC and severe UC showed an increasing trend (9:11:115). Moreover, Bacillus, Lactococcus and Alloprevotella disappeared in UC. Parabacteroides and a type of Prevotella disappeared in severe UC. Serious dysbacteriosis and abundant putative pathogenic bacteriа are the two important features of severe UC. The percentage phyla and the abundance of putative pathogenic bacteria could be used to predict the evolution tendency.

Identification and quantitation of isomeric pneumococcal polysaccharides by partial chemical degradation followed by mass spectrometry

Quantitation of isomeric pneumococcal polysaccharides in vaccines is a challenging task due to mixture complexity, their low quantities, and identical monosaccharide compositions. Differentiation and quantitation of isomeric pneumococcal polysaccharides were investigated here based on a partial chemical degradation mass spectrometry approach to generate an oligosaccharide marker for one isomer, and not the other. Mild base conditions were successful at generating unique ions for the isomers with the weakest glycosidic bonds, while strong base and acid conditions were successful at generating unique ions for the more stable isomers. Linear relationships between the ion abundance of the oligosaccharide marker and the starting pneumococcal polysaccharides concentration were established for all isomers. Furthermore, precision measurements for each method were below 12% demonstrating good robustness. Therefore, partial chemical degradation followed by mass spectrometry was successful at differentiating and quantifying isomeric pneumococcal polysaccharides and may be adopted for other bacterial types.

The clinical value of procalcitonin cut-off levels for pediatric patients in the hospital setting.

Utilization of procalcitonin (PCT) is challenging for hospital pediatricians because of uncertainty in clinical interpretation. We used a PCT decision cut-off value (<0.15 ng/mL) to identify if PCT can differentiate bacterial infections from viral and other conditions in pediatric patients who presented for hospital-based care. This retrospective study included PCT tested patients who presented to our children's hospital from 2017 to 2020. We analyzed relevant demographic, laboratory, treatment, and clinical data, including discharge diagnoses consolidated into bacterial infections, viral syndromes, and other conditions by the highest PCT defined as ≤0.15 ng/mL (Group A) or >0.15 ng/mL (Group B). We used regression models to identify factors associated with PCT above decision limits and the role of PCT levels in the duration of antibiotic therapy. Of 238 patients, 32.8% constituted Group A. Bacterial infections represent 25.6% of diagnoses for patients in Group A and 55% for Group B (P<0.001), however, the distribution of bacterial infection types, including bacteremia, was comparable. Number of PCT tests performed and C-reactive protein (CRP) ≥5 mg/L, but no other factors were significantly associated with PCT >0.15 ng/mL. PCT levels did not predict the length of antibiotic therapy, which depended on duration of hospitalization and increased CRP. PCT as a single measurement above or below a decision cut-off value of 0.15 ng/mL does not specify bacterial infections or predict the duration of antibiotic therapy in hospitalized pediatric patients.

Keanekaragaman dan Prevalensi Bakteri Pada Karapas Cuora amboinensis (Riche in Daudin 1801) Asal Habitat Alam

The aim of this research is to identify the diversity of bacterial types and determine the prevalence value of these bacteria on the carapace of C. amboniensis from natural habitats to support the provision of data that can later be used to support captive management activities and conservation actions C. amboniensis was collected manually and 20 individuals were obtained, samples were caught in December 2019. Data from bacterial identification was then used to calculate the prevalence of bacterial diversity. The identification results showed that there were 7 types of bacteria, most of which were Gram negative bacteria. The prevalence values were then calculated to obtain the results of A. caviae (5%), C. jejuni (30%), C. freundii (15%). ), F. breve (25%), L. monocytogenes (15%), P. haemolytica type A (5%), and P. alcaligenes (5%).

Bioinspired Topographic Surface Modification of Biomaterials.

Physical surface modification is an approach that has been investigated over the last decade to reduce bacterial adhesion and improve cell attachment to biomaterials. Many techniques have been reported to modify surfaces, including the use of natural sources as inspiration to fabricate topographies on artificial surfaces. Biomimetics is a tool to take advantage of nature to solve human problems. Physical surface modification using animal and vegetal topographies as inspiration to reduce bacterial adhesion and improve cell attachment has been investigated in the last years, and the results have been very promising. However, just a few animal and plant surfaces have been used to modify the surface of biomaterials with these objectives, and only a small number of bacterial species and cell types have been tested. The purpose of this review is to present the most current results on topographic surface modification using animal and plant surfaces as inspiration to modify the surface of biomedical materials with the objective of reducing bacterial adhesion and improving cell behavior.

Decline in the number of patients with meningitis in German hospitals during the COVID-19 pandemic

Background and objectivesIn 2020, a wide range of hygiene measures was implemented to mitigate infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In consequence, pulmonary infections due to other respiratory pathogens also decreased. Here, we evaluated the number of bacterial and viral meningitis and encephalitis cases during the coronavirus disease 2019 (COVID-19) pandemic.MethodsIn a multicentre retrospective analysis of data from January 2016 until December 2020, numbers of patients diagnosed with bacterial meningitis and other types of CNS infections (such as viral meningitis and encephalitis) at 26 German hospitals were studied. Furthermore, the number of common meningitis-preceding ear-nose-throat infections (sinusitis, mastoiditis and otitis media) was evaluated.ResultsCompared to the previous years, the total number of patients diagnosed with pneumococcal meningitis was reduced (n = 64 patients/year in 2020 vs. n = 87 to 120 patients/year between 2016 and 2019, all p < 0.05). Additionally, the total number of patients diagnosed with otolaryngological infections was significantly lower (n = 1181 patients/year in 2020 vs. n = 1525 to 1754 patients/year between 2016 and 2019, all p < 0.001). We also observed a decline in viral meningitis and especially enterovirus meningitis (n = 25 patients/year in 2020 vs. n = 97 to 181 patients/year between 2016 and 2019, all p < 0.001).DiscussionThis multicentre retrospective analysis demonstrates a decline in the number of patients treated for viral and pneumococcal meningitis as well as otolaryngological infections in 2020 compared to previous years. Since the latter often precedes pneumococcal meningitis, this may point to the significance of the direct spread of pneumococci from an otolaryngological focus such as mastoiditis to the brain as one important pathophysiological route in the development of pneumococcal meningitis.

Methods to Evaluate Bacterial Motility and Its Role in Bacterial–Host Interactions

Bacterial motility is a widespread characteristic that can provide several advantages for the cell, allowing it to move towards more favorable conditions and enabling host-associated processes such as colonization. There are different bacterial motility types, and their expression is highly regulated by the environmental conditions. Because of this, methods for studying motility under realistic experimental conditions are required. A wide variety of approaches have been developed to study bacterial motility. Here, we present the most common techniques and recent advances and discuss their strengths as well as their limitations. We classify them as macroscopic or microscopic and highlight the advantages of three-dimensional imaging in microscopic approaches. Lastly, we discuss methods suited for studying motility in bacterial–host interactions, including the use of the zebrafish model.

Point-of-care urine Gram stain led to narrower-spectrum antimicrobial selection for febrile urinary tract infection in adolescents and adults

BackgroundFebrile urinary tract infections (fUTIs), which include pyelonephritis, prostatitis, and urosepsis, are the most common cause of sepsis. However, the treatment has become more complex because of the worldwide increase in antimicrobial resistance (AMR). The objective of this study was to clarify whether point-of-care Gram stain (PCGS) of urine contributed to fUTI diagnosis and treatment in adults.MethodsThis hospital-based observational study was undertaken between January 2013 and March 2015 in Okinawa, Japan. All enrolled patients were adults who had been admitted to the Division of Infectious Diseases with suspected fUTI. The usefulness of PCGS results were compared for urinalysis (U/A) and urine cultures (U/Cs). The targeted therapy type and its susceptibility based on PCGS were analyzed, and each was investigated in two groups: the uncomplicated pyelonephritis group and the complicated pyelonephritis/prostatitis group.ResultsTwo hundred and sixty-six patients were enrolled. The results of PCGS were closely correlated with those of U/A for pyuria and bacteriuria, and moderately correlated with the results of U/C for bacterial types. In the uncomplicated group, narrow-spectrum antimicrobials such as cefotiam were initially selected in 97.9% (47/48) of patients, and their susceptibility was 97.9% (47/48). In the complicated group, the susceptibility was 84.2% (186/221) (p = 0.009) despite frequent AMRs (14.7%; 32/218) and low use of broad-spectrum antimicrobials such as carbapenems (7.7%; 17/221).ConclusionUrine PCGS led to a more precise fUTI diagnosis and prompted clinicians to select narrower-spectrum antibiotics with high susceptibility.

Nutrients Removal from Aquaculture Wastewater by Biofilter/Antibiotic-Resistant Bacteria Systems

The presence of nutrients in aquaculture water bodies causes serious problems for aquatic ecosystems, requiring the development of technical solutions to reduce their amounts. This work identifies some bacterial strains that are able to reduce nutrient quantities and studies the effect of antibiotics on bacterial growth, on the ability to form biofilms on different solid supports, and on the ability to reduce nutrients in aquaculture wastewater. Bacterial biofilm adhesion on different substrates depends on the surface properties of the substrate, on the bacterial types that form the biofilm, but also on biofilm–substrate interactions. The obtained results revealed the high capacity of biofilm-forming bacterial strains of Pseudomonas aeruginosa and Chromobacterium violaceum to eliminate up to 87% of nitrate and nitrite ions and up to 62% of phosphate ions in the presence of concentrations of 0.1–0.4 µg/mL of oxytetracycline antibiotic from aquaculture wastewater.