Survey the bacterial types in breast tissue and CA13-5 for women with breast disease in Babylon province

Abstract

Breast tumor may be malignant and benign so these tumor and breast diseases are harboring bacteria ,but types of bacteria may differ among breast tumors so the current study was aimed to detect type of bacteria in breast tissue . Specimens( Breast tissue and blood) was collected from 50 female (aged ranged between 14 to 60 ) undergoing breast surgery at AL-Hilla Teaching Hospital and Al-Sadiq Hospital in Babylon Province during period from November 2021 - March 2022 and blood sample also collected from healthy women as control . tissue obtained was combined outside the marginal zone, approximately 5 cm away from the neoplasm. After enucleation, the new tissue was immediately placed in a sterile plane tube or urine cup it contain a normal saline solution and it was cut , homogenized by using a sterile surgical scalpel and wooden sticks within 30 min of collection.2 it was cultivated on blood agar &MacConkey media ,and then on selective media .and Identification for bacteria by microscopic ,biochemical test and confirmed by Vitec 2Compact System . The results appeared the percentage of malignant tumor (17.14%) and benign (82.85%) , The growth percentage (88.57%) while no growth was (11.42%).